1.Effect of inactivated SARS coronavirus vaccine on mouse organs
Bin DU ; Xueyun ZHONG ; Sheng XIONG ; Chuanhai ZHANG ; Xinjian LIU ; Shisheng LIU ; Meiying ZHANG ; Jiuxiang LI ; Yifei WANG ; Jiahai LU ; Zhuoyue WAN ; Xinge YAN ; Huanying ZHENG ; Jianglin FAN
Chinese Journal of Pathophysiology 2000;0(07):-
AIM: To study the pathological change in mouse organs immunitied by inactivated SARS-CoV vaccine. METHODS: Inactivated SARS-CoV vaccine was injected into BALB/c and C57BL/6 mice. Anti-SARS antibody was analyzed by ELISA. After 8 weeks, the immunitied mice were killed and those organs were analyzed by pathological methods. RESULTS: Anti-SARS antibody in mice was positive after 8 days. Only minimal injury was observed in a few lungs and livers, but the other organs were not. CONCLUSIONS: Inactivated SARS-CoV vaccine induced mice to create antibody, whereas they did not cause severe injury. This result will be valuable for vaccine into clinical research. [
2.Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA.
Renli ZHANG ; Zhongmin GUO ; Jiahai LU ; Jinxiu MENG ; Canquan ZHOU ; Ximei ZHAN ; Bing HUANG ; Xinbing YU ; Min HUANG ; Xinghua PAN ; Wenhua LING ; Xigu CHEN ; Zhuoyue WAN ; Huanying ZHENG ; Xinge YAN ; Yifei WANG ; Yanchao RAN ; Xinjian LIU ; Junxin MA ; Chengyu WANG ; Biliang ZHANG
Chinese Medical Journal 2003;116(8):1262-1264
OBJECTIVETo evaluate the effectiveness of small interfering RNA (siRNA) on inhibiting severe acute respiratory syndrome (SARS)-associated coronavirus replication, and to lay bases for the future clinical application of siRNA for the treatment of viral infectious diseases.
METHODSVero-E6 cells was transfected with siRNA before SARS virus infection, and the effectiveness of siRNA interference was evaluated by observing the cytopathic effect (CPE) on Vero-E6 cells.
RESULTSFive pairs of siRNA showed ability to reduce CPE dose dependently, and two of them had the best effect.
CONCLUSIONsiRNA may be effective in inhibiting SARS-associated coronavirus replication.
Animals ; Cercopithecus aethiops ; RNA, Small Interfering ; pharmacology ; SARS Virus ; drug effects ; Transfection ; Vero Cells ; Virus Replication ; drug effects
3.Laboratory detection on severe acute respiratory syndrome
Jicheng HUANG ; Zhuoyue WAN ; Qiuxia CHEN ; Hui LI ; Kui ZHENG ; Huanying ZHENG ; Xinge YAN ; Xin ZHANG ; Ling FAN ; Jie LI ; Xiaoling DENG ; Huiqiong ZHOU ; Ping HUANG ; Limei DIAO ; Haojie ZHONG ; Wanli ZHANG ; Shaoying XIE ; Jingdiao CHENG ; Jian WANG ; Jinyan LIN ; Feng DENG
Chinese Journal of Laboratory Medicine 2003;0(10):-
Objective To provide scientific evidence to identify and confirm severe acute respiratory syndrome (SARS) by laboratory detection.Methods Multiple clinical specimens were collected serially and systematically from the 4 suspected SARS patients, which occurred between Dec.2003 to Jan.2004 in Guangdong Province. The samples were tested by serologic and molecular methods.Results IgM or IgG antibodies against SARS-CoV were detectable after 6—8 days of the onset in four patients. The four-fold or greater rising in antibodies was clearly detected in three of the four patients, while the fourth patient’s seroconversion was from negative to positive. The results analysed by enzyme-linked immunosorbent assay( ELISA), immunoflourescence assay (IFA), and neutralization test were highly correlated. SARS-CoV RNA was just detected in 3 throat swab specimens from case 1 by real-time PCR. M, N and S genes were amplified by reverse transcriptase polymerase chain reaction (RT-PCR) from the positive samples. Sequencing results showed that they were SARS-CoV gene segments, and most closely matched SARS-CoV gene sequences were isolated from civet cats in Guangdong Province. Nevertheless, SARS-CoV was not isolated from any samples of the 4 patients.Conclusion Based on these results, the 4 reported cases were laboratorily confirmed as SARS cases.