1.Level and clinical significance of RNA oxidative damage in human gastric cancer and para-carcinoma tissues
Xianhui LIN ; Junhua YU ; Haihong LIN ; Yangyang TU ; Shunbin LUO ; Tao XU ; Tong CHEN ; Xingcheng LIN ; Sunzhi YE ; Zhiqiang ZHENG ; Jianping CAI
Chinese Journal of Pathophysiology 2015;(1):172-176
AIM:To investigate the RNA oxidative damage in human gastric cancer tissue and para-carcinoma tissue for exploring the role of RNA oxidation in the occurrence of gastric cancer .METHODS:Immunohistochemical ob-servation and LC-MS/MS analysis were performed in 61 cases of gastric carcinoma .The position and concentration of 8-oxoguanosine ( 8-oxoGsn ) were detected , respectively . RESULTS: The results of immunohistochemical observation showed that 8-oxoGsn was obviously up-regulated in the gastric cancer .The positive staining mainly accumulated in the cy-toplasm of the tumor cells .The results of mass spectrometry showed that the level of 8-oxoGsn in the gastric cancer tissues was higher than that in the para-carcinoma tissues (P<0.05).CONCLUSION:8-oxoGsn is up-regulated in gastric canc-er.RNA oxidative damage may play important roles in the occurrence of gastric cancer .
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