The adverse effects on the ocular surface caused by long-term antiglaucomatous medications or filtering surgery have been documented by many researchers.However,in our clinical work emphasis on this issue is far from enough.Both antiglaucomatous drugs and filtering surgery can cause tear film dysfunction and conjunctival or corneal epithelium damage,which is the main cause of glaucomatous patients'ocular uncomfortable complaints.Early diagnosis and prompt management of the ocular surface dysfunction is of great importance for us to improve patients' local tolerance and decrease their ocular uncomfortable symptoms.

Objective:To evaluate the results of treating Perthes disease by pelvic osteotomy and internal fixation with self-reinforced absorbable (SR-PLLA) rods. Methods:50 cases of Perthes disease(53 hips) were involved. According to Herring classification, there were 16 hips of type B ?5 hips of type B/C and 32 hips of type C. All patients were treated by pelvic osteotomy(Chiari)and internal fixation with self-reinforced absorbable (SR-PLLA) rods. Results:All cases were followed up for 2 months to 18 months, with an average of 6 months. All cutting bone was healed. There was no bacterial wound infection or sterile inflammatory foreign-body reaction. Failure of fixation was only occurred in one case. Bone union was seen 6 weeks to 12 weeks, with an average of 8 weeks. All patients restored normal hip joint function and had no pain. Conclusion:This operative technique is simple and can achieve reliable fixation. It can improve bone healing with a reduced healing time and avoid secondary a removal operation. This surgical procedure is an effective way to treat cutting pelvis in Perthes disease.

Objective:To compare characters of urethral healing after different surgical procedures to provide theoretical support for preventing and curing urethral scaring stricture after hypospadias repair.Methods:Hypospadiac animal model was established and three surgical procedures were adopted to repair urethra.The dynamic changes of urethral healing and scar were observed,the expressions of ?-SMactin and TGF-?1 were examined.Results:Mathieu and Onlay island flap(Onlay) had notable collagenous proliferation,which was significantly stronger than that of Tubularized Incised Plate urethroplasty(TIPU)(P

The hemodynamics of ocular artery (OA) and central retinal artery(CRA)in normal persons and patients (102 eyes, respectively)with primary open-angle glaucoma (POAG) were studied with color Doppler image technique. The results showed that average values of peak systolic flow velocity (Vmax), the end-diastolic flow velocity (Vmin) and the mean flow velocity (Vmeau)for the OA and CRA in glau-comatous patients we^e much lower than that in normal persons. But the mean resistance index (RI) was higher than that in normal persons. The linear regression analysis presented that there was a positive correlation of Vmin between the OA and CRA (r = 0.3278, p

The doctor-patient relationship is always the focus in the society. The relationship seems more complex and sensitive in the pediatric surgical ward because its patients are kids. However, the pediatric surgical residents' ability of doctor-patient communication is lack of systemic and norma-tive training, which is not good for the mutual understanding between doctors and patients. This study explores how to enhance the residents' doctor-patient communication skills from the perspective of awareness, professional knowledge and some specific skills. In the meantime, it discusses the need to train the teachers and set up a regular examination-assessment system.

Quantum dots( QDs) as a new type of nanostructured luminescent materials have been widely used in biology, materials science, and physical optics. QD-based biosensors can rapidly and accurately detect biological macromolecules or inorganic molecules both in vivo and in vitro. The surface of QDs, prior to their biological detection applications, needed to be modified to enhance their fluorescence properties and lower their biological toxicity. Currently, the surface modification technologies mainly include polymer modification, thiol compounds modification, mercapto propionic acid compounds modification and organic groups modification. Various biosensors can be developed by adopting different modification techniques, which have been adopted to locate and track a variety of biological molecules in vivo. Although a large number of literatures have reported the biological applications of QD-based biosensors, rare systematic reviews of surface modification technologies on QDs have been witnessed. In this paper, we reviewed the surface modification technologies of QDs in biosensors and their medical applications.

BACKGROUND:The hedgehog pathway has paid an important role in the progress of chondrogenesis from bone marrow mesenchymal stem cells. However, the definite signal transduction pathway and cross-talking relationship with other common signal pathways are stil poorly understood and the researches related to this field is to continue as a hotspot in the future study. OBJECTIVE:To investigate the research progress of hedgehog signal pathway on the regulation of the chondrogenesis from bone marrow mesenchymal stem cells and the relationship between hedgehog and other signal pathways in the process. METHODS:A computer-based online search in CNKI, PubMed and Google Scholar databases was performed using key words of“Hedgehog, IHH, SHH, bone marrow mesenchymal stem cells, cartilage, chondrogenesis”in English and Chinese, respectively. Literatures related to the process of chondrogenesis from bone marrow mesenchymal stem cells were included and 36 articles were extensively summarized for review. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cells are currently accepted optimal cellseeds for the cartilage tissue engineering, and hedgehog is a critical signal molecule in the development of skeletal system. The IHH and SHH in hedgehog signal closely participate in control ing the processes of bone marrow mesenchymal stem cellproliferation and chondrogenesis, chondrocyte phenotype maintenance and cooperation with other common single pathways. However, the specific signal transduction mechanism and cross-talking contact with other signal pathways stil need to be further studied, and it stands for the future research directions.

The view of "Harmony between Man and Nature" in Song-Ming period,taking "the same rule of man and nature" as mode,"clearing the rules according examining the things" as means,made the doctors of vital gate school in traditional Chinese medicine set up a life pattern of "taiji(vital gate)-yin and yang-five elements(zang-fu viscera)",so as to explore the origin of life and mystery.The study on the characteristics of view of "Unity of Heaven and Man" in different historical stages is conducive to grasp the track and skeleton of the formation of traditional Chinese medical theories,lay the foundation for the further development of traditional Chinese medicine.

Objective To review the examination techniques and the current research progress of the magnetic resonance diffusion weighted-imaging (DWI) used in liver. Methods The recent and relevant literatures about the principles and the current study situation of liver DWI were scrutinized and analyzed retrospectively. In addition, the existing problems of liver DWI were discussed. Results DWI could demonstrate the normal and abnormal structure and function through measuring the diffusion motions of water molecule in the liver. With the improving technology and better understanding of diffusion dynamics, DWI has been used for the diagnosis and differential diagnosis for hepatic diseases. Conclusion DWI as a non-invasive examine method, may provide valuable functional information for clinical diagnosis and treatment.

Background and purpose: NF-?B is activated by tumor necrosis factor,some chemotherapeutic agents,and ionizing radiation.The activation of NF-?B could result in inhibition of apoptosis.NF-?B,regulated by PDTC(pyrrolidine dithiocarbamate),is a specific inhibitor of NF-?B.The purpose of our study was to explore the impact of inhibiting expression of NF-?B on radiation-induced apoptosis of uterine cervix cancer HeLa cells.Methods:Inhibition of NF-?B in HeLa cells was performed by treatment with 100 ?mol/L PDTC for 2 hours.Cells were irradiated at 0,2,4 and 6 Gy with or without PDTC.The expression of NF-?B in nuclear was determined by western blot,and apoptosis was evaluated by using the TUNEL assay.Cell proliferation was evaluated by using MTT assay.Results:NF-?B activation was induced by radiation and inhibited by PDTC.Inhibition of radiation-induced NF-?B activation resulted in inhibiting cell proliferation(P