ObjectiveTo explore the feasibility and safety of the spleen-preserving surgery in the treatment of traumatic rupture.Methods47 patients with traumatic rupture were treated with spleen-preserving surgery:the biological glue which local physical bleeding had 7 cases,suture repair had 13 cases,splenic artery ligation had 10 cases,splenic artery ligation and splenic repair had 9 cases,partial splenectomy had 8 cases.Results47 patients had four cases of infection,and cured after dressing,1 case had postoperative bleeding symptoms and used splenectomy surgery.46 patients with spleen-preserving operation is successful,the average length of stay was 15 days,there is no deaths.After 6 months,all patients used abdominal B-utrasoundor CT scans to confirm the survival of spleen is good and indicator of the examination is normal.Follow-up of these patients had no dangerous infections and complications.ConclusionSpleen-preserving surgery in the treatment of traumatic rupture was safe,feasible,could retain the greatest degree of function,and could be used in clinical practice.

China ; Humans ; Occupational Health ; Occupational Medicine ; Research

Humans ; Silicosis ; prevention & control

Apoptosis ; drug effects ; Asbestos ; toxicity ; Humans ; Mineral Fibers ; toxicity

Objective:To explore the behavior and eye movement characteristics when processing different emotional faces in college students with different levels of social anxiety.Methods:According to the scores of Liebowitz social anxiety scale, 26 and 27 subjects with high and low social anxiety were selected.The response time and accuracy of the subjects under consistent and inconsistent conditions were measured by the dot probe task.Eye movement data of the subjects were recorded when they viewed angry, disgusting and neutral faces.SPSS 19.0 software was used for repeated measurement analysis of variance.Results:The accuracy rates of the high social anxiety group were (0.97±0.10) and (0.93±0.15) under consistent and inconsistent conditions, and the response time of the high social anxiety group were (660.97±125.38) ms and (687.81±150.90) ms under consistent and inconsistent conditions.High social anxiety subjects showed significant differences in the accuracy and response time under different consistency conditions ( F(1, 51)=15.25, P<0.01 for accuracy, F(1, 51)=7.85, P<0.01 for reaction time), while low social anxiety subjects showed no significant difference(both P>0.05). There were significant differences between the first fixation latency for emotional faces and neutral faces( F(2, 102)=15.01, P<0.01). The total fixation time of emotional face and neutral face was significantly different ( F(2, 102)=4.38, P<0.05). Conclusion:College students with different levels of social anxiety all showed early attentional alertness and attentional disengagement difficulty to emotional faces, but only those with high social anxiety showed attentional bias to emotional faces at the behavioral level.

Objective: To study the chemical constituents from the pericarps of Aquilaria sinensis and their cytotoxic and antibacterial activities. Methods: The compounds were isolated by various chromatographic methods (silica gel, reverse silica gel, Sephadex LH-20, preparative TLC, and so on) and recrystallization. Their structures were identified by extensive analysis of the spectroscopic data. The cytotoxic effects of these compounds on neuroglioma SF-268, breast cancer MCF-7, large cell lung cancer NCI-H460, and hepatoma carcinoma HepG-2 cell lines were observed in vitro by SRB staining, and the antibacterial effects of these compounds on Staphyloccocus aureus and Escherichia coli were observed in vitro by Resazurin staining method. Results: Ten compounds were isolated from the pericarps of A. sinensis and identified as isorhamnetin 3-O-(6″-O-(Z)-p-coumaroyl)-β-D-glucopyranoside (1), buddlenoid A (2), 7-methoxy-4'-hydroxyisoflavone (3), luteolin (4), trans-p-coumaric acid ethyl eater (5), costunolide (6), epifriedelanol (7), stigmasterol (8), p-hydroxybenzoate (9), and pyrocatechol (10). Conclusion: Compounds 1-3, 5, 6, 9, and 10 are first isolated from the plant and compounds 1-3, 5, and 6 are isolated from the plants of genus Aquilaria Lam. for the first time. Compound 6 exhibits the significant cytotoxic activities against the above four cell lines and compounds 1 and 4 show some antibacterial activities against S. aureus and E. coli.

The view of "Harmony between Man and Nature" in Song-Ming period,taking "the same rule of man and nature" as mode,"clearing the rules according examining the things" as means,made the doctors of vital gate school in traditional Chinese medicine set up a life pattern of "taiji(vital gate)-yin and yang-five elements(zang-fu viscera)",so as to explore the origin of life and mystery.The study on the characteristics of view of "Unity of Heaven and Man" in different historical stages is conducive to grasp the track and skeleton of the formation of traditional Chinese medical theories,lay the foundation for the further development of traditional Chinese medicine.

Objective:To study the clinical efficacy and safety of BCG-polysaccharide nucleic acid combined with isotretinoin soft capsules in the treatment of plane warts. Methods:Seventy patients with plane warts were randomly divided into the observation group and the control group(n=35). The control group were treated with isotretinoin soft capsules, limp 10mg, bid, 4 weeks to 10mg, qd. The observation group were given BCG-polysaccharide nucleic acid 1ml, im, every otherday, on the basis of the contrl group. After 8 weeks of treatment, T cell populations and immunoglobulin levels were detected, and the efficacy and adverse reactions were recorded. Results:After the treatment, T cell population level, IgG and IgA of the two groups were significantly improved(P<0. 01), and the improvement in the observation group was significantly better than that in the control group (P<0. 05). The onset time of the observa-tion group was shorter than that of the control group ( P<0. 01). The total effective rate of the observation group was higher than, that of the control group(P<0. 05). The occurrence of adverse reactions in the two groups were no significant(P>0. 05). Conclusion:The clinical efficacy of BCG-polysaccharide nucleic acid combined with isotretinoin soft capsules in the treatment of plane warts is sig-nificant with promising security.

Objective To study the biological effects of hirudin on the cell circle of fibroblasts in hypertrophic scar.Methods Fibroblasts were taken from normal skin and hypertrophic scar,and cultured in vitro with various concentrations of hirudin.Then,flow cytometry was used for observing cellcycles,and Western blot for some proteins (p27 and cyclin E) related with cell circle.Results With the increasing of the concentrations of hirudin,fibroblasts on G1 phase increased and on S phase decreased,and the expression of protein p27 increased,but that of protein cyclin E decreased.Conclusion Hirudin can influence the cell circle of fibroblasts derived from normal skin and hypertrophic scar by means of the expression of some proteins related with celI circle.Hirudin causes G1 phase arrest and inhibits the production of fibroblasts.Therefore,it seems that hypertrophic scar could be prevented and treated with hirudin.

OBJECTIVE： To investigate the possible role of the alteration of mitochondrial transmembrane potentials(Δ Ψ m) in arsenic trioxide(As2O3)-induced apoptosis of human gastric adenocarcinoma cells(SGC7901).METHODS： Cell apoptosis was assessed through morphology， cell viability， the percentage of sub-G1 cells and phosphatidylserine(PS) externalization.the Δ Ψ m was detected on flow cytometry through double staining of Rhodamine 123 (Rh123) and propidium iodide(PI).RESULTS： The As2O3-induced apoptosis was associated closely with the disruption of the Δ Ψ m.CONCLUSION： The disruption of the Δ Ψ m is one of the mechanisms of As2O3-induced apoptosis.