BACKGROUND:Extracorporeal shock wave (ESW) therapy is using principles of liquid-electric conversion and transfer to produce energy gradient difference and torsional tension in tissues with varied density.It is a matured technique in treating urinary and digestive system calculosis.However,the applications of ESW in treating nonunion or delayed osseous union are few.OBJECTIVE:To explore the therapeutic effects of ESW in treating nonunion or delayed osseous union by animal experiment and clinical verification.METHODS:The effect of ESW on periosteal metabolism was explored by focusing extracorporeal wave on bone tissues,and the autoradiography of H~(3-)TdR labeling rate was observed by animal experiment.ESW was used to treat nonunion patients (n=16) or delayed osseous union patients (n=16),and the follow-uP results were evaluated.RESULTS AND CONCLUSION:By autoradiography,the H~(3-)TdR labeling rate was greater in the experimental sides than that of the control sides at 1 and 2 weeks after operation (P＜0.05-0.01),which confirmed that the mitotic activity of periosteal osteoblasts was increased,namely,endosteal osteogenesis was enhanced.Clinical verification results demonstrated that,at 16 weeks after operation,16 patients with delayed union were all cured,13 were cured among the 16 cases of nonunion,and the remained 3 cases had notably callus formation.No adverse events occurred in all cases.The results suggested that it is reliable to treat nonunion or delayed osseous union using ESW therapy.

Objective To investigate the therapeutic effect of Xiaoxuan Kangfu capsule in treating psoriasis vulgaris. Methods All patients were divided into two groups randomly (60 patients in a control group and 60 patients in a treatment group). In the control group complex Qingdai capsule was given to the patients, with 4 pills for each time and 3 times daily. While in treatment group Xiaoxuan Kangfu capsule was given to the patients with 6 pills for each time and twice daily. 30 days consisted one treatment period for both groups. The patients undertook one to three treatment period according to the patients' pathogenetic condition. The rates of recovery and improvement and were compared. Results In the treatment group, 34 patients were cured (42.5%), and the total effective rate was 87.5%; while in the control group 14 patients were cured (7.5%), and the total effective rate was 72.5%. There was statistic difference between the two groups (P＜0.05). The average heal time of the treatment group was shorter than that of the control group, showing statistic difference (P＜0.05).Conclusion Xiaoxuan Kangfu capsule showed higher recovery rate, shorter healing time and lower recurrence rate in treating psoriasis vulgaris than complex Qingdai capsule. The treatment is deserving clinical spread.

ObjectiveTo identify the chemical composition of stem, leaf and peel ofCynanchumchinese R.Br..MethodsFourier IR spectra, the second derivative spectrum and two-dimensional correlation spectroscopy were adopted to identify IR of different parts ofCynanchumchinese R.Br..ResultsIt was found that the IR spectra of the leaf was similar to that of the peel but was different from the stem. What’s more, in the second derivative spectra of the leaf, it showed that the absorption peak was strong at the position of 1 543 cm-1, 1 515 cm-1, 1 499 cm-1 and 1 467 cm-1, respectively and there existed carbonyl absorption peak at 1 738 cm-1 and 1 659 cm-1 in addition. Therefore, it was inferred that flavonoids were the major components while less in the stem and peel.ConclusionsThe differences of the three parts aboutCynanchumchinese R.Br. were studied through IR spectrum macroscopically, which provided reference for exploring the constituents and clinical medication.

China ; Humans ; Oral Health ; Oral Hygiene ; Periodontal Diseases ; complications ; Practice Guidelines as Topic

China ; Humans ; Occupational Health ; Occupational Medicine ; Research

This paper reported that the nitrite concentration, pH value, total living bacteria and the percentage of nitrate-reducing bacterium strains in the gastric juice of the residents in Cangshan county who usually take garlic were significantly less than those in Qixia county who rarely take garlic. It was also shown that garlic homogenate, ethyl ethanethiosulfonate (an active principle of garlic) all could inhibit the growth of nitrate-reducing bacteria and their production of nitrite in vitro. This may be considered as a protective factor against gastric cancer.

Objective To construct a recombinant AAV reporter virus expressing GFP and observe its expression in NIH 3T3 cell. Methods The resulting gene of GFP was subcloned into the site EcoRⅠand BamHⅠof vector pssHGCMV, and a recombinant adeno-associated virus (rAAV) vector pssHGCMV-GFP expressing GFP was constructed by using DNA recombinant technique. The 143 cells were transfected with the vector and the rAAV stock expressing GFP was packaged, produced and purified. The titre of the rAAV stock was measured by quantitative dot blot hybridization. NIH 3T3 cells were infected with the rAAV and the fluorescence was detected in alive cells to confirm successful infection and the expression of exogenous gene in NIH 3T3 cell line. Results A GFP-expressing rAAV vector VssCMV-GFP was successfully constructed. The titer of the VssCMV-GFP was 3.16?10~(12) particles/mL. GFP was initially expressed in the NIH 3T3 cells 48h following infected by VssCMV-GFP and subsequently it gradually increased. The green fluorescence in the cells was the most apparent 120h after infected. Conclusion VssCMV-GFP can infect the eukaryotic cells and GFP can be expressed in the alive cells.It is suggested that rAAV-mediated transfer of the gene to the eukaryotic cells can serve as a delivery system of gene therapy.

Objective To understand the effects of Erigeron Breviscapus(EB) on suture osteogenesis during maxillary protraction in animal models.Methods The experiment was carried in 16 pubertal rabbits using self-made distraction devices during maxillary protraction,through which a forward elastic force(about 350 g) was exerted.During maxillary protraction,the experimental group underwent Erigeron Breviscapus,injected about(0.4mL) at either side of premaxilla-maxillary suture every day,and the control group were injected physiological saline.Undecalcified tissue slices labeled by serial tetracycline fluorochrome for 28 days were prepared for examination of the newly formed bone by computer image analysis.The results were compared between the two groups.Results The quantity of newly formed bone was significantly different between the two groups.The experimental group showed more newly formed bones than the control group.Conclusion EB can promote suture osteogenesis during maxillary protraction in the model.

Four test methods of HEPA filter such as D.O.P test, sodium flame test, oil mist test and particle counting test,are introduced briefly.Furthermore the numerical relations of the above four methods are analyzed. The differences between efficiency of test and practical efficiency and the aspects needed to be considered when testing filter for bio-protection are discussed at the end.

By introducing the job contents classification of hospital, the paper analyzes each sub-system including its information contents, structure features and construction factors, discusses the plan for establishing electronic medical records with combination of the component of medical records, from sequencing of the system, management mode, noted issues, moreover, suggests that each hospital should accord to own situation establishing own system.