Object A method was developed for the determination of fatty acid in Fructus Bruceae oil by precolumn derivation HPLC Methods Fatty acids were derivatized with p bromophenacylbromide as derivative and 18 crown 6 as catalyst The method used C 8 Kromasil C 8 (250 mm?4 mm, 5 ?m) column and isocratic acetonitrile water eluent and the internal standard was heptadecanoic acid The detection wavelenghth was 254 nm Column temperature was fixed at room temperature, and the flow rate was 1 3 mL/min Results The standard curves of linoleic, palmitic, oleic, and steraric acid are linear within the range of 0 022—0 330, 0 014—0 213, 0 028—0 416, and 0 012—0 177 ?g, respectively The four fatty acid recoveries are 99 2%, 97 2%, 101 8%, 97 8%, and the RSD are 1 2%, 1 5%, 0 4%, 2 3%, individuslly Conclusion Five fatty acids are separated within 30 minutes during a single run The present method is reliable and relatively simple for the determination of fatty acid in Fruetus Bruceae oil

Objective To determine the pathogen profile and antibiotic resistance in aerobic isolates from blood cultures of neonates. Methods All blood culture reports (n=28120) from newborns admitted to the Department of Neonatology during 2002-2012 were analyzed, and the sensitivity patterns were recorded. Results A total of 1665 bacteria were isolated from 1606 blood culture-positive samples and the positive rate of blood cultures was 5.7%(1606/28120). Gram-positive bacteria were iso-lated in 1336 cases, with Staphylococcus epidermidis (902 cases) and Staphylococcus haemolyticus (206 cases) being the com-mon bacteria. Klebsiella pneumoniae (108 cases), followed by Escherichia coli (73 cases), were the major Gram-negative bacte-ria (235 cases). The determination of the antibiotic resistance of aerobic isolates was performed in 2012. Most Gram-positive iso-lates were sensitive to vancomycin and moxifloxacin, and more than 90%were resistant to penicillin while most of Gram-nega-tive isolates were sensitive to amikacin and imipenem. Conclusions Staphylococcus epidermidis, Staphylococcus haemolyticus, Klebsiella pneumoniae and Escherichia coli remain to be the principal organisms responsible for neonatal sepsis.

OBJECTIVE: To facilitate hospital service reform as well as the implementation of Prescription Management Method.METHODS: The advantages and disadvantages of pricing of prescriptions by financial staff versus dispensing staff were compared.RESULTS & CONCLUSIONS: The financial staff lay stress on economical income but the dispensing staff on rationality of prescriptions,and the latter plays an indispensible in preventing medical risk and ensuring patients' medication safety.It is advisable to establish network for the contents of drug prices and financial fee-collecting and to facilitate a cooperative office system of financial pricing staff and pharmacists.

Child ; Humans ; Neoplasms ; Quality of Life

Cellulase system contains a series of complex components.There are still some problems remained unclear in cellulase and its mechanism of hydrolyzing lignocellulosic materials and its hydrolysis kinetics,so profound study is needed.The rapid development of many kinds of new interdiscipline such as biochemistry,molecular biology and gene engineering,has further clarified the structure and function of cellulase,and the relationshi Pof its gene expression and regulation,and furthermore resulted in derivative study methods about cellulase in more aspects.Cellulase system components according to synergistic catalytic mode and the sequence of the homology amino acids similarity,summarizes traditional detection methods of enzyme components,and emphasizes on research progress of various biosensors applied in detection of cellulase activity and gene expression was introduced.

Objective To investigate clinical characteristics of 48 cases verified to be infected with S.suis type 2.Methods 1.All data of 48 cases suffered from S.suis type2 infection were col- lected and analyzed.2.Pathogenic gene of S.suis type 2,Such as cps 2A,mrp,and sly et al.,were verified by PCR.Results 1.Pathogenic gene of S.suis type 2 were same from those patients and swine.Drug sensitivity test were carried on and showed resistance to tetracycline and streptomycin. 2.All 48 cases had history to butchering and/or direct contacting blood plasma composition of suffer- ing from or dead pigs.People with wound in the skin had higher risk to be infected.3.Four clinical types were classified as general,meningitis.Shock and both shock combined meningitis.Mortality rate was 14.58%.Conclusion 1.S.suis type 2 was the pathogen leading to the infections of 48 cases in this study.The swine of suffering from the disease or dead were the origins of the transmis- sion.2.Main route of infection was butchering or/and direct contacting the plasma composition of be- ing ill or dead pigs.No second generation of patients were found.3.The cases with shock should be treated as early as possible.4.Taking antibiotic were rational used seriously in human being and ani- mals.

Acute pancreatitis(AP) is mainly caused by gallstone disease and excessive alcohol consumption.Overall, about one fifth of patients develop into severe acute pancreatitis( SAP), which is still associated with a mortality rate exceeding 30%. This type of AP is usually accompanied by necrosis of the pancreas and/or organ failure. Treatment of SAP in several aspects, such as the application of antibiotics, nutritional support, surgical time, the method of operation, are still in dispute. In recent years, minimally invasive technique is increasingly used in the treatment of SAP, and some patients may benefit from the adoption of minimally invasive surgery.This article aims to review the current progress on the treatment of SAP.

AIM: To delevop new drug delivery system for volatile oil of lignum dalbergia odoriferae and to improve its effect. METHODS: Solid-lipid-nanopaticals were prepared by hot-dispersiom technique and treated further by sonication; the contents of dispersion and entrapment efficiency of nanoparticles were determined by HPLC. RESULTS: Solid lipid nanoparticles containing volatile oil of lignum dalbergia odoriferae was spherelike; The partical size was 40.0nm; the content of dispersion was 72.1%; entrapment efficiency of nanoparticles was 91.27%. CONCLUSION: Solid-lipid-nanopatical may be a suitable drug delivery system for volatile oil of lignum dalbergia odoriferae.

Objective To prepare Tongshuan Jiuxin pH-Dependent Delayed-release Capsula and study its pharmacokinetics and pharmacodynamics in dogs in vivo. Methods Borneol and the extracts of Cortex Moutan, Rhizoma Chuanxiong, and Rhizoma Cyperi prepared by CO2 supercritical fluid extraction method were solidified or included with ?-cyclodextrin. Pellets were prepared by means of powder layering with the solidified extracts and borneol. The pH-dependent delayed-release pellet system was prepared by coating with HPMC, Eudragit L-30D-55, and Eudragit L100-S100 (1∶5) combinations, respectively. Tongshuan Jiuxin pH-Dependent Delayed-release Capsula was prepared by mixing the three kind of pellets at the ratio of 1∶1∶1. The concentration of paeonol in plasma was determined by HPLC and the pharmacodynamic parameters were evaluated by serum pharmacology method. Results The results from the paeonal concentration in plasma showed that the mean residence time (MRT) in vivo were 8.04 h for Tongshuan Jiuxin pH-Dependent Delayed-release Capsula and 2.89 h for Tongshuan Jiuxin Tablet, and the relative bioavailability was 126.3%. Meanwhile, the results from the pharmcodynamic parameters evaluated by serum pharmacology method showed that the MRT were 11.27 h for Tongshuan Jiuxin pH-Dependent Delayed-release Capsula and 5.94 h for Tongshuan Jiuxin Tablet, and the relative bioavailability was 129.1%. Conclusion Tongshuan Jiuxin Tablet has the characteristics of rapid absorption, qucik elimination, and shorter action-maintaining time. Whereas Tongshuan Jiuxin pH-Dependent Delayed-release Capsula has the characteristics of rapid absorption, prolonged and relaxed effective action compared with Tongshuan Jiuxin Tablet.