Objective To determine the pathogen profile and antibiotic resistance in aerobic isolates from blood cultures of neonates. Methods All blood culture reports (n=28120) from newborns admitted to the Department of Neonatology during 2002-2012 were analyzed, and the sensitivity patterns were recorded. Results A total of 1665 bacteria were isolated from 1606 blood culture-positive samples and the positive rate of blood cultures was 5.7%(1606/28120). Gram-positive bacteria were iso-lated in 1336 cases, with Staphylococcus epidermidis (902 cases) and Staphylococcus haemolyticus (206 cases) being the com-mon bacteria. Klebsiella pneumoniae (108 cases), followed by Escherichia coli (73 cases), were the major Gram-negative bacte-ria (235 cases). The determination of the antibiotic resistance of aerobic isolates was performed in 2012. Most Gram-positive iso-lates were sensitive to vancomycin and moxifloxacin, and more than 90%were resistant to penicillin while most of Gram-nega-tive isolates were sensitive to amikacin and imipenem. Conclusions Staphylococcus epidermidis, Staphylococcus haemolyticus, Klebsiella pneumoniae and Escherichia coli remain to be the principal organisms responsible for neonatal sepsis.

OBJECTIVE: To facilitate hospital service reform as well as the implementation of Prescription Management Method.METHODS: The advantages and disadvantages of pricing of prescriptions by financial staff versus dispensing staff were compared.RESULTS & CONCLUSIONS: The financial staff lay stress on economical income but the dispensing staff on rationality of prescriptions,and the latter plays an indispensible in preventing medical risk and ensuring patients' medication safety.It is advisable to establish network for the contents of drug prices and financial fee-collecting and to facilitate a cooperative office system of financial pricing staff and pharmacists.

Object A method was developed for the determination of fatty acid in Fructus Bruceae oil by precolumn derivation HPLC Methods Fatty acids were derivatized with p bromophenacylbromide as derivative and 18 crown 6 as catalyst The method used C 8 Kromasil C 8 (250 mm?4 mm, 5 ?m) column and isocratic acetonitrile water eluent and the internal standard was heptadecanoic acid The detection wavelenghth was 254 nm Column temperature was fixed at room temperature, and the flow rate was 1 3 mL/min Results The standard curves of linoleic, palmitic, oleic, and steraric acid are linear within the range of 0 022—0 330, 0 014—0 213, 0 028—0 416, and 0 012—0 177 ?g, respectively The four fatty acid recoveries are 99 2%, 97 2%, 101 8%, 97 8%, and the RSD are 1 2%, 1 5%, 0 4%, 2 3%, individuslly Conclusion Five fatty acids are separated within 30 minutes during a single run The present method is reliable and relatively simple for the determination of fatty acid in Fruetus Bruceae oil

Child ; Humans ; Neoplasms ; Quality of Life

Acute pancreatitis(AP) is mainly caused by gallstone disease and excessive alcohol consumption.Overall, about one fifth of patients develop into severe acute pancreatitis( SAP), which is still associated with a mortality rate exceeding 30%. This type of AP is usually accompanied by necrosis of the pancreas and/or organ failure. Treatment of SAP in several aspects, such as the application of antibiotics, nutritional support, surgical time, the method of operation, are still in dispute. In recent years, minimally invasive technique is increasingly used in the treatment of SAP, and some patients may benefit from the adoption of minimally invasive surgery.This article aims to review the current progress on the treatment of SAP.

AIM: To delevop new drug delivery system for volatile oil of lignum dalbergia odoriferae and to improve its effect. METHODS: Solid-lipid-nanopaticals were prepared by hot-dispersiom technique and treated further by sonication; the contents of dispersion and entrapment efficiency of nanoparticles were determined by HPLC. RESULTS: Solid lipid nanoparticles containing volatile oil of lignum dalbergia odoriferae was spherelike; The partical size was 40.0nm; the content of dispersion was 72.1%; entrapment efficiency of nanoparticles was 91.27%. CONCLUSION: Solid-lipid-nanopatical may be a suitable drug delivery system for volatile oil of lignum dalbergia odoriferae.

Objective To investigate the in vivo pharmacokinetics of ligustilide in Ligusticum chuan-xiong essential oil after ig administration in rats. Methods The concentration of ligustilide in plasma of rats after ig administration of ?-cyclodextrin compound in L. chuanxiong enssential oil was determined by RP-HPLC method with the osthole as the internal standard. The plasma concentration-time curve was plotted. The main pharmacokinetic parameters of ligustilide were obtained by 3P97 software. Results Ligustilide in rats fits the two compartments model and the main pharmcokinetic parameters were as follows: tl/2(?), (1.429?1.161) h; tl/2(?), (6.877?2.275) h; t(peak), (3.401?1.951) h; and AUC were (70.87?25.92) ?g/mL?h, respectively. Conclusion RP-HPLC Method with osthole as the internal standard is accrurate, sensitive, and rapid for the study on pharmacokinetics of ligustiliden in rats in vivo.

OBJECTIVE:To study the relationship between various composition factors and the dissolution rates of the soft gelatin capsule shell.METHOD:The gelatin disk and gelatin shaped mass method were used by the current CP rotating-basket dissolution apparatus.The effects of shell compositions on the capsule shell dissolution rate were illustrated and discussed.RESULTS:With the increase in the ratio of gelatin glycerol in soft gelatin shell,the dissolution rate of the shell changed irregularly.After storage of 21 days at 40℃，the dissolution rate of the shell decreased at different extent.The dissolution rate of the shaped gelatin mass increased slightly by adding some polymers (pvp,starch).CONCLUSION:Am investigation of these factors and their influence on dissolution may be of assistance in the formulation of soft gelatin capsule shells for various purposes,THE SOFT gelatin capsules should be stored at relatively low temperature.

Objective To explore the clinical application of improved thoracic closed drainage tube vacuum aspiration in treatment of elderly patients with trachea foreign body.Methods 32 elderly patients with trachea foreign body were divided evenly into group A and group B.Group A received treatment of improved thoracic closed drainage tube aspiration,while group B received treatment of bronchoscopy.One-time success rate of removing foreign body,operation duration,length of stay and occurrence of complications were compared between group A and group B.Results One-time success rate of removing foreign body in group A was significantly higher than group B (100.00％ vs 81.25 ％,x2 =6.720,P ＜ 0.05) ;operation time of group A was (1.79 ± 1.25)min,which was significantly shorter than (13.18 ± 4.37) min of group B (t =4.31,P ＜ 0.05).Conclusion The clinical effect of improved thoracic closed drainage tube vacuum aapiration in treatment of elderly patients with trachea foreign body was positive and better than treatment with bronchoscopy,it is worthy of clinical application.