Attention Deficit Disorder with Hyperactivity ; diagnosis ; Humans

Objective:To explore the effects of antisense oligonucleotide(ASODN)of connective tissue growth factor (CTGF)on the apoptosis of human hypertrophic scar fibroblasts(HSF)and the related mechanisms.Methods:The hypertrophic scar fibroblasts were cultured in vitro and were divided into the following 3 groups:CTGF-ASODN-treated group, liposome control group and blank control group.The distribution of CTGF-ASODN in HSF of different phases was observed under fluorescent microscope in 3 groups.Flow cytometer was employed to detect the apoptotic rate of HSF and the expression of Fas.The expression of CTGF,Fas and bcl-2 mRNA in HSF was assessed by RT-PCR.Results:24 h after transfection,large quantity of yellow-green fluorescence was seen in the HSF cytoplasm of CTGF-ASDON group in a scattered or doted manner, but not found in that of the other 2 groups.The apoptosis rate of HSF in CTGF-ASDON group was significantly higher than those in the other 2 groups(P

The doctor-patient relationship is always the focus in the society. The relationship seems more complex and sensitive in the pediatric surgical ward because its patients are kids. However, the pediatric surgical residents' ability of doctor-patient communication is lack of systemic and norma-tive training, which is not good for the mutual understanding between doctors and patients. This study explores how to enhance the residents' doctor-patient communication skills from the perspective of awareness, professional knowledge and some specific skills. In the meantime, it discusses the need to train the teachers and set up a regular examination-assessment system.

Child ; Child Behavior ; Child Behavior Disorders ; diagnosis ; Child Development ; Developmental Disabilities ; diagnosis ; Humans ; Infant ; Intellectual Disability ; diagnosis ; Pediatrics ; Severity of Illness Index ; Terminology as Topic

Behavioral Medicine ; Child ; Child Behavior ; Child Behavior Disorders ; diagnosis ; prevention & control ; psychology ; Child Development ; Child Health Services ; trends ; China ; Developmental Disabilities ; diagnosis ; prevention & control ; History, 20th Century ; History, 21st Century ; Humans ; Pediatrics ; history ; trends ; Prospective Studies ; Psychology, Child

The development in Chinese child health care demands the communication with the society of education.Bio-education is a new promising subject integrating bio-science with education.It is the evidence-based science that combines biology,neuro-physiology,neuro-psychology,brain science with education to study the educational phenomenon of mankind and its principles.It is a bridge through which bio-medicine communicates with education.This new subject will be helpful to build up the new model of integrating medicine with education.It will accelerate both of the basic and applicable research in children's growth and development,learning ability,social adaptive ability and health improvement.Hence,either the field of bio-medicine or education will thrive on the newly founded subject.

Attention Deficit Disorder with Hyperactivity ; Child ; Child Behavior Disorders ; Child Development Disorders, Pervasive ; Developmental Disabilities ; Humans ; Pediatrics ; education ; methods ; Societies, Medical

Animals ; Computational Biology ; Digestive System Neoplasms ; metabolism ; Electrophoresis, Gel, Two-Dimensional ; Humans ; Mass Spectrometry ; Proteomics ; methods

Animals ; Animals, Newborn ; Antioxidants ; administration & dosage ; pharmacology ; Apoptosis ; drug effects ; Brain ; drug effects ; pathology ; Disease Models, Animal ; Female ; Hypoxia-Ischemia, Brain ; drug therapy ; metabolism ; pathology ; Immunohistochemistry ; Male ; Melatonin ; administration & dosage ; pharmacology ; Neuroprotective Agents ; administration & dosage ; pharmacology ; Rats ; Rats, Sprague-Dawley

Objective To evaluate the anti-tumor activity of interferon ? with different structures including pegylated interferon ?-1b (PEG IFN ?-1b), pegylated interferon ?-2b (PEG IFN ?-2b) and natural interferon ? (IFN ?) in male BALB/C nude mice with human renal adencarcinoma (ACHN) tumor. Methods Athymic BALB/C nude mice were received a subcutaneous injection of ACHN cells (2?106/0.2ml) on the rear flank. After four weeks, the size of tumor reached a mean volume of 50-100 mm3, and the interferon was administrated. The mice with tumor were divided into four groups, and were administrated with placebo (0.2ml/mouse thrice weekly), IFN ?-1b (50?g/mouse thrice weekly), PEG IFN ?-1b (150?g/mouse once weekly) and PEG IFN?-2b (150?g/mouse once every week), respectively, for five weeks. The tumor volumes were measured weekly prior to treatments until the end of the fifth week. All mice in each group were sacrificed to measure the weight of the residual tumor. The anti-tumor activity of IFN ? in vivo was evaluated by studying their ability to reduce the volume and weight of tumor. Results IFN ? may significantly reduce the volume and weight of ACHN tumor in mice. The average tumor weight was 0.422g in placebo group, while 0.263g, 0.235g and 0.219g in the groups treated with PEG IFN ?-1b, IFN ?-1b and PEG IFN ?-2b, respectively. No significant difference was found among the three groups treated with IFN ?. Moreover, the volume of tumor in three groups treated with IFN ? was significant reduced than that in placebo group. Conclusion IFN ? with different structures have showed the similar ability of ACHN tumor suppression at the dosage of 150?g per week.