Significant progress has been made toward understanding the tumorigenic role and clinical significance of the T1799A B-type Raf kinase(BRAF)mutation in papillary thyroid cancer(PTC). With these advances, it has become clearer that BRAF mutation will likely have significant impact on the clinical management.

Many researches proved that inflammation plays a decisive role in occurrence, development and prognosis of acute coronary syndrome (ACS).Atorvastatin possesses many other effects besides lipid regulation, and anti-inflammatory effect is the most significant one.Some studies indicated that atorvastatin can reduce levels of high sensitive C reactive protein (hsCRP), interleukin (IL)-6 and tumor necrosis factor (TNF)-α in ACS patients, then inhibit postoperative inflammation, improve postoperative blood flow perfusion and reduce occurrence of acute coronary events.The present article made a review on influence of atorvastatin on levels of hsCRP, IL-6 and TNF-α in ACS patients.

Objective To investigate the effects and mechanism of Shufeng Decoction resisting type I hypersensitivity. Methods Type I hypersensitivity was induced by OVA. The content of IL-4, IFN-? and IgE in serum of the rats treated by Shufeng Decoction were determined by ELISA. Results Shufeng Decoction can increase the content of IFN-? in model rats (P0.05). IFN-?/IL-4 of Shufeng Decoction was significant higher than model group (P0.05). Conclusion The effects and mechanism of Shufeng Decoction on prophylaxis and treatment of type I hypersensitivity of skin (acute urticaria) may be related with regulating cell factors and influencing the balance of Th1/Th2.

BACKGROUND:The thickness of ceramic materials plays a crucial role in the reappearance of veneer restorations. Due to the limited space for the construction of porcelain veneer, it is difficult to cover the abutment tooth color, and the final color after restorations is achieved through the overlapping of prosthetic restorations color and abutment tooth color. In recent years, there is little evidence on the effect of veneer materials on the color. OBJECTIVE:To evaluate the color matching of porcelain veneer restorations with different ceramic materials to shade tab. METHODS:Fifteen veneer restorations were fabricated by three ceramic materials (VITA Mark II, Ivoclar E.max LTCAD and Ivoclar E.max CAD Multi, A2 shade). Veneer restorations were controled at the thickness of 0.6 mm. The color parameters of veneer restorations were measured by Olympus Crystaleye against the natural color shade resin material substrate. The color differences were calculated between veneer restoration and shade tab (A2 shade) at the cervical, central and incisal sections, respectively. The color differences (?E values) were statisticaly analyzed using a one-way analysis of variance. RESULTS AND CONCLUSION:The color value of E.max CAD LT block was the closest one among the three blocks to the standard shade guide. The results of one-way analysis of variance for the ?E values showed that, there were significant differences between the cervical sections of the veneer restorations and the shade guide (P< 0.05). At the cervical sections, the ?E values between three kinds of blocks with the standard shade guide showed significant differences (P < 0.05); the differences were also significant in L values at the central and incisal sections, a values at the incisal sections and b value at the shoulder sections (P < 0.05). Our findings indicate that, there are significant differences in the color of porcelain veneer restorations with different ceramic materials to shade tab. The difference is also found among the three sections of the veneer restorations. In the clinical application, the final prosthesis color effects should be noted, which can change by surface staining and bonding technology.

Objective To investigate the effects and mechanism of Xiaomin granules on type Ⅰ hypersensitivity.Methods The experimental animal model was made for type Ⅰ hypersensitivity.The mice were divided into five groups named vacant control group,Xiaomin granules(low-,middle-and high-dose) group,chlorpheniramine group randomly and were given homologous medication respectively.Results Xiaomin granules can inhibit capillary penetrance caused by histamine and 5-HT in mice(P 0.05).Xiaomin granules could inhibit the mice hind paw edema degree caused by histamine.The further study found that Xiaomin granules could decrease anaphylactic shock of the mice and decrease the death rate.Conclusions Xiaomin granules could restrain type Ⅰ hypersensitivity by inhibiting inflammatory mediators.

Objective To systematically assess the relationship between oral administration of aspirin and survival benefit for patients with colorec?tal cancer(CRC)by meta?analysis. Methods Relevant studies were identified through searching PubMed and EMBASE. Random?effects model was derived to composite the pooled hazard ratio for overall mortality and CRC?specific mortality. The subgroup analysis was conducted for included data,and the bias analysis was reported. Results Thirteen studies on aspirin therapy were finally included in this meta?analysis. The overall surviv?al benefit associated with oral administration of aspirin represented an HR of 0.83(95%CI:0.71?0.96). Oral administration of aspirin was also asso?ciated with CRC?specific survival(HR=0.77,95%CI:0.64?0.93). No evidence was observed of an association between prediagnostic aspirin use and CRC overall survival(HR=1.00,95%CI:0.85?1.18)or CRC?specific survival(HR=0.97,95%CI:0.83?1.13). Conclusion These findings provide further indication that post?diagnostic aspirin therapy can improve CRC prognosis.

Adult ; Aged ; Cardiovascular Agents ; therapeutic use ; Diastole ; drug effects ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Heart Failure ; drug therapy ; physiopathology ; Humans ; Injections ; Male ; Middle Aged ; Phytotherapy ; Systole ; drug effects ; Ventricular Dysfunction, Left ; complications ; drug therapy

Objective To investigate the effect of liver phosphatidylinosital 3-kinase/protein kinase B (PI3-K/AKT) pathway on the decrease of insulin sensitivity in fetal growth restriction (FGR) rats.Methods Twenty pregnant female rats were randomly divided into two groups one day after conception:normal-protein group and low protein group (n=10,respectively).Rats in low-protein group was given low protein diet (8.00％ protein) during pregnancy to build FGR model,while normal-protein group was given normal protein diet (20.00％ protein).On day 3,7,14,30,60 and 90 after birth,fasting blood samples of 8 male FGR offsprings from low-protein group and 8 normal offsprings from normal-protein group were collected to measure fasting plasma glucose and insulin level.Then insulin resistance index and insulin sensitivity index were calculated to determine insulin sensitivity.On day 7,14,30,60 and 90 after birth,liver tissue of 8 male FGR and normal offsprings were collected,insulin receptor substrate 1,2 (IRS1/IRS2)and glucose transporter 4 (GLUT4) mRNA expression were measured by real-time fluorescence polymerase chain reaction and the protein expressions of IRS1,PI3-K (subunit p110β),and AKT and phosphorylated AKT (pAKT) were measured by Western blot.The relationships between the expression changes of key molecules of PI3-K/AKT pathway and insulin sensitivity were analyzed by correlation and multiple linear regression method.Results (1) Mean birth weight of baby rats in low-protein group was significantly lower than that of normal-protein group [(4.92±0.36) g vs (6.43±0.59) g,t=14.73,P＜0.05].The incidence of FGR in low-protein group was 88.2％ (97/110); and for male offsprings,it was 94.1 ％ (48/51).(2) Compared to normal offsprings,fasting plasma glucose levels of male FGR offsprings were significantly higher from the age of 60 days to 90 days.Insulin levels and insulin resistance index were significantly higher and insulin sensitivity index was lower from the age of 30 days to 90 days,P＜0.05 respectively.(3) Compared to normal offsprings,IRS1 (0.45 ± 0.02 vs 0.68± 0.03,t=16.633,P＜0.05) and IRS2 mRNA (0.34±0.10 vs 0.70±0.19,t=4.864,P＜0.05) expressions in FGR offsprings were lower from day 7 after birth to day 90 (0.48±0.03 vs 0.59±0.05,t=5.237,P＜0.05; 0.49±0.20 vs 0.70±0.11,t=2.253,P＜0.05).There were no differences in expressions of GIUT4 mRNA and AKT protein between two groups (P＞ 0.05).IRS1,PI3-K and pAKT protein expressions of FGR offsprings decreased significantly from day 14 (0.22±0.05 vs 0.52±0.11,t=7.024,P＜0.05; 0.46±0.03 vs 0.97±0.08,t=17.508,P＜0.05; 0.62±0.10 vs 0.89±0.08,t=6.100,P＜0.05) to day 90 (1.11±0.08 vs 1.32±0.14,t=3.714,P＜0.05; 0.63±0.07 vs 1.00±0.19,t=5.206,P＜0.05;0.28±0.03 vs 0.45±0.10,t=4.880,P＜0.05).(4) The pAKT protein expression level of FGR rats was positively correlated with insulin sensitivity index (r=0.704,P＜0.05) ; while negatively correlated to the level of fasting plasma glucose (r=-0.609,P＜0.05),fasting insulin (r=-0.561,P＜0.05) and insulin resistance index (r =0.577,P＜ 0.05).Conclusions The changes of some key molecules' expressions of PI3-K/AKT pathway in liver might be involved in the insulin resistance in FGR rats.

The LEAFY (LFY) homologous gene of Dendrobium moniliforme (L.) Sw. was cloned by new primers which were designed based on the conservative region of known sequences of orchid LEAFY gene. Partial LFY homologous gene was cloned by common PCR, then we got the complete LFY homologous gene Den LFY by Tail-PCR. The complete sequence of DenLFY gene was 3 575 bp which contained three exons and two introns. Using BLAST method, comparison analysis among the exon of LFY homologous gene indicted that the DenLFY gene had high identity with orchids LFY homologous, including the related fragment of PhalLFY (84%) in Phalaenopsis hybrid cultivar, LFY homologous gene in Oncidium (90%) and in other orchid (over 80%). Using MP analysis, Dendrobium is found to be the sister to Oncidium and Phalaenopsis. Homologous analysis demonstrated that the C-terminal amino acids were highly conserved. When the exons and introns were separately considered, exons and the sequence of amino acid were good markers for the function research of DenLFY gene. The second intron can be used in authentication research of Dendrobium based on the length polymorphism between Dendrobium moniliforme and Dendrobium officinale.

Liposome is an artificially prepared spherical vesicle that has a phospholipid bilayer. Given that the basic structure of its biological membrane is also a lipid bilayer membrane, liposome shares similar structures with body cells Therefore, liposome has good biocompatibility and advantages such as biodegradability, low immunogenicity, and subtle toxicity. Liposome has been widely ap-plied as an effective drug carrier. Studies on liposome-encapsulated fluorescent dye on tumor tracing have been reported in recent years. Liposome can become a more advantageous transport carrier with continuous development of surface modification materials and prepa-ration methods. The long cycle, targeted liposome-encapsulated drugs, and fluorescent dye have become the focus of interest for several researchers. This article mainly discusses the application and progress of long cycle and targeted liposome in cancer research.