Objective To investigate the alteration of chaperonine hsp40 and its influence on delayed death of neurons in the CA1 region of hippocampus after transient cerebral ischemia. Method After transient global ischemia for 20 minutes, rat model was made. Following different lengths of reperfusion, all the 28 wistar rats were divided into sham-operation group,4 hour recovery group, 24 hour recovery group and 72 hour recovery group ( n = 7 rats in each group). Immunochemistry and laser scanning confocal microscopy were used to observe the distributional alteration of hsp40 in the neurons. Differential centrifuge and western blot assay were used to analyze the quantitative alteration of hsp40 and its redistribution in the neurons. Results Immunochemistry and laser scanning confocal microscopy showed the progressive reduction of hsp40 occurred at first in the cytosol, then in the nucleus until the death of all the neurons in the CA1 region died. Differential centrifuge and western blot assay showed the level of hsp40 decreased from 1.00 ± 0.21 to 0.23 ± 0.13 ( P ＜ 0.01 ) 24 hours after reperfusion; the quantity of hsp40 in the protein aggregates increased from 1.00±0.18 to 8.61 ± 1.89 (P ＜0.01 =24 h after reperfusion.Conclusions The reduction of hsp40 in the neurons of hippocampus CA1 region is an important role in protein aggregates formation.

Objective To explore the impact factors of liver uptake of 18F-FDG in PET/CT imaging,and to preliminary analyze the application of maximum standardized uptake values corrected by conventional body mass,lean body mass and body surface area (SUVmax-B,SUVmax-L,SUVmax-S).Methods A retrospective analysis was performed on 67 healthy subjects who underwent 18F-FDG PET/CT.SUVmax-B,SUVmax-L and SUVmax-S were measured,and the impact of age,levels of fasting blood glucose,body mass index (BMI),CT value of liver,gender and the fatty liver on SUVmax-B,SUVmax-L and SUVmax-S were analyzed.Results The liver SUVmax-B,SUVmax-L,SUVmax-S were positively correlated with the levels of fasting blood glucose (r=0.329,0.336,0.353,P =0.012,0.010,0.007).There was a positive correlation between BMI and SUVmax-B (r=0.543,P＜0.01),SUV-L (r=0.328,P=0.007),while BMI value had no correlation with SUVmax-S (r=0.026,P=0.833).There was no correlation between age,CT value of the liver nor SUVmax-B,SUVmax-L and SUVmax-S (all P＞0.05).The liver SUVmax-B (t=2.608,P=0.011) and SUVmax-L (t=5.272,P=0.001) of male were significantly higher than those of female,while there was no difference between male's and female's liver SUVmax-S and liver SUVmax-S (t=0.759,P=0.450).There was no difference in SUVmax-B,SUVmax-L nor SUVmax-S between fatty liver subjects and non-fatty liver subjects (P=0.646,0.775,0.068).Conclusion The fasting blood glucose level,BMI and sex are the impact factors of liver uptake of 18F-FDG in PET/CT imaging.SUVmax-B can be replaced by SUVmax-L or SUVmax-S for larger BMI subjects,while SUVmax-S can make up differences between different genders.However,correction techniques can not weaken the impact of blood glucose level on SUVmax value of liver.

Objective To observe the 18F-FDG PET/CT manifestations of primary systemic anaplastic large cell lymphoma(ALCL).Methods A total of 21 patients with primary systemic ALCL were enrolled,and PET/CT manifestations were observed.Results Among 21 cases of ALCL,there were 15 cases of ALK+and 6 cases of ALK-.Affected lymph nodes in multiple site were observed in 19 cases,mainly located in the neck(n=13),mediastinum(n=12 cases)or retroperitoneum(n=12),while single site affected lymph node was notice in 2 cases.Extranodal organs/site involvements were found in 12 cases,including 6 cases of soft tissue(such as skin,muscles,etc.),4 cases of bone,14 cases of organs,including 4 cases of lung,3 cases of liver,2 cases of pancreas,2 cases of kidney,2 cases of gastrointestinal tract and 1 case of thyroid.Among 21 cases of ALCL,19 with irregular lymph node morphology and fused into clusters,17 with uniform density,3 with necrosis and 1 with calcification.All ALCL lesions in 21 cases showed hypermetabolism,the maximum standard uptake value(SUVmax)and the mean standard uptake value(SUVmean)of the affected lymph node was 17.04±9.94 and 9.96±6.15,respectively,while the metabolic tumor volume(MTV)and total lesion glycolysis(TLG)of all lesions was 92.54(67.61,249.21)cm3 and 723.46(419.78,1 461.17)g,respectively.The maximum diameter of the affected lymph node was not significantly correlated with SUVmax nor SUVmean(both P＞0.05),but positively correlated with MTV and TLG of all lesions(r=0.696,0.767,both P＜0.001).Ann Arbor staging was positively correlated with the maximum diameter,SUVmax and SUVmean of the affected lymph node,also MTV and TLG of all lesions(r=0.467,0.458,0.702,0.780,0.664,all P＜0.05).Conclusion 18F-FDG PET/CT manifestations of primary systemic ALCL were characteristic,with significant changed metabolic parameters,including SUVmax,SUVmean,MTV and TLG.