Objective] To observe the clinical efficacy of Agkistrodon in treating Sjogren's syndrome. [Methods] 52 patients were randomly divided into two groups: Treatment of 26 patients taking Agkistrodon and the basic prescription medicine(YIQIYANGYINQUYU drugs); 26 patients in the control received the basic prescription medicine. The clinical symptoms,the disease activity score of SSDAI,the score of TCM symptom and life quality and the index of serology were recorded in this clinical observation. [Results] The treatment group had better results of immune function than the control group after treatment, in particular had a substantial decrease in IgG. In addition, the clinical symptoms, the disease activity score of SSDAI, the score of TCM symptom and life quality were improved. [Conclusion] Agkistrodon has significantly immunosuppressive effects on the patients with Sjogren's syndrome , thus improving the patients' condition,the standard of living and the quality of life.

Objective To investigate the charaeteristcs of the acute poisoning patients in the emergency department of a general hospital in a new emerging district by analyzing the data of Hangzhou.Methods A retrospective study of the characteristics of the poisoning patients in emergency department of Tongde Hospital of Zhejiang Provinee from January 2004 to December 2004 was conducted and statistically analyzed.Results There were 318 poisoning patients in this year, accounting for 8.3% of the total patients in emergency department.Among these poisoning patients,female was 58.8% and male 41.2%;patients aged 21～30 years old accounted for 53.3%;alcoholic poisoning was the major cause and accounted for 34.3%,and 83.3% were male;as the second eanso,sedative accounted for 34.0%,and 72.4% were female;food poisoning accounted for 6.91%,and students was 90.9%.There was no relation between season and poisoning type.In the poisoning patients,30.8% were career men,and 19.5% were unemployed,19.6% were students,and 85.2 % were deliberate.Conclusion Young persons and career men were the high-risk group of poisoning, poisoning type was connected with work and gender.

Objective:To study the effects of Jiaweitaohechengtang on expression of?-SMA protein in hepatic fibrosis rats induced by CCl4.Methods:Hepatic fibrosis models were induced by CCl 4 .All the experimental rats were divided into six groups:normal control group,model control group,colchicine positive treatment group,jiaweitaohechenqitang groups with high- dosage,medium-dosage and low-dosage.Drugs were given to rats for 8 weeks by intragastric administration.Scores of hepatic fibrosis were assessed with semiquantitive scoring system(SSS)through tianlanghong staining.Protein expression levels of ?-smooth muscle actin(?-SMA)were determined with immunohistochemical steining method.Results:SSS in model control group were higher than those in normal control group,but lower than those in three treatment groups with jiaweitaohechengqitang. Compared with nornal control group,the protein expression levels of?-SMA in liver tissues in model control group increased markedly,but decreased when it was compared to jiaweitaohechengqitang groups.There was a significant difference between model control group and three treatment groups.Conclusion:Jiaweitaohechengqitang has therapeutic effects on hepatic fibrosis.The mechanism may be ralated to the inhabitition of the activation of HSC.

Objective To detect the different expression of Runx2 in dedifferentiated chondrosarcoma and conventional chondrosarcoma, and to investigate the role of Runx2 in the occurrence and development of dedifferentiated chondrosarcoma. Methods Dedifferentiated chondrosarcoma cell line NDCS-1 and normal chondrosarcoma cell line SW1353 were cultured, then mRNA and total cellular protein were extracted.RT-PCR Western blotting, and immunocytochemistry were used to detect the expression of Runx2.Immunohistochemistry was used to test Runx2's expression in the dedifferentiated chondrosarcoma specim ens that confirmed by pathology. Results Runx2 was high expression in dedifferentiated chondrosarcoma cell line and high-grade component of dedifferentiated chondrosarcoma tissues. Conclusion The high expression of Runx2 in dedifferentiated chondrosarcoma is involved in the occurrence and development of dedifferentiated chondrosarcoma.

Bone marrow mesenchymal stem cells (BMSCs) is a kind of multipotent adult stem cells,which is one of the most important seed sources of tissue engineering.Microgravity has inhibitory effects on osteogenic differentiation of BMSCs,which will cause bone mass reduction and changes of bone micro-structure that finally lead to osteoporosis.This process is regulated by multiple signaling pathways such as mitogen-activated protein kinase (MAPK) pathway,Notch pathway and Wnt/β-catenin pathway which co-regulated BMSCs osteogenic differentiation under microgravity.Studying the effects of microgravity on BMSCs into osteogenic differentiation can clarify the mechanism of bone loss,put forward new targets for the treatment of diseases and provide a useful reference for the development of China's space industry.

Objective To evaluate the effects of traspupillary thermotherapy (TTT) for the treatment of central exudative chorioretinopathy (CEC). Methods Twelve eyes of 12 patients who suffered from CEC were treated by using a diode laser at 810 nm. A variable spot size of 0.5 mm 2 0 mm was used depending on the size of choroidal neovascularization (CNV).The treatment was initiated in one spot for 55 to 60 seconds duration at a power range between 200 350 mW, and the treated area revealed no visible color change to a light gray appearance. Preoperative and postoperative fluorescein angiography (FFA) and indocyanine green angiography (ICGA) were performed in 10 of the 12 eyes. Results The visual acuity in all eyes was improved to different degree over a period of 3 10 months. Five or more lines improvement measured by Snellen chart in visual acuity was found after the treatment in 4 eyes, three to five lines in 5 eyes and one to two lines in 3 eyes. All eyes demonstrated decreased or vanished exudation in FFA. CNV disappeared or decreaced in 8 eyes and remained nochange in 2 eyes in ICGA. Conclusion TTT is obviously effective in treating CEC.

AIM To study antitumor activity of the extract denominated as UAP(Uric Antitumor Peptides isolated and purified from normal human urine). MOTHODS Proliferation inhibition assay was examined by cell counting and MTT method. Experiments in vivo were tested by treatment on tumor mice and acute toxicity on healthy mice. RESULTS UAP exerted strong dose dependent inhibition on the proliferation of three tumor cell lines, but not human normal leucocytes. Treatments on tumor mice show that UAP effectively inhibited tumor growth of mice HAC hepatoma, mice Lewis lung carcinoma and mice S 180 sarcoma with apparently dose dependent effect. Acute toxicity proved that UAP had very low acute toxicity in mice with LD 50 = 2 137 13 mg?kg -1 . CONCLUSION UAP has strong antitumor activity in vitro and in vivo. This provides a promising alternative antitumor treatment.

Objective To explore the effects of Jiedu Quyu Ziyin Recipe (JQZR) on the apoptosis and expressions of bcl-2 and bax mRNA of peripheral-blood lymphocyte in MRL/lpr mice. Methods 80 MRL/lpr mice were randomly divided into model group,TCM group, Western medicine group and TCM and Western medicine group,20 mice in each group, meanwhile,20 Kunming mice were selected as normal group, then intragastrically administered normal sodium, JQZR apozem, prednisone suspension and JQZR apozem and prednisone suspension, 0. 5ml every time,once daily for 12 weeks respectively. At the end of the 12th week, peripheral-blood lymphocytes of every mice purified by gradient centrifugation were cultivated for 48 hours ,then the apoptosis was detected by flow cytometry. Furthermore,the expressions of bcl-2 and bax mRNA of peripheral-blood lymphocyte were detected by RT-PCR. Results At Oh or 48h,the apoptosis ratios of PBLC in normal group, TCM group,Western medicine group and TCM and Western medicine group are higher than model group and the differences are significant(P <0. 05 ,P <0. 01 ) ,while,at Oh,which are not significant within Western medicine group, normal group and TCM and Western medicine group ( P > 0. 05) ,even if which are significant between TCM group and TCM and Western medicine group or between model group and TCM and Western medicine group( P < 0. 05 ). At 48h, the differences of apoptosis ratios of PBLC are significant between Western medicine group and TCM and Western medicine group( P < 0. 05 ), and between TCM group and TCM and Western medicine group or between model group and TCM and Western medicine group which are significant, too( P < 0. 01 ). The ratios of bcl-2/bax mRNA of normal group,TCM group and TCM and Western medicine group are significantly lower than which of model group or Western medicine group ( P < 0. 05, P < 0. 05, P <0. 01 ,respectively) ,but there is no significant difference between which of model group and Western medicine group.Conclusion JQZR has adjustable effects on the apoptosis and expressions of bcl-2 and bax mRNA of peripheralblood lymphocyte in MRL/lpr Mice.

Objective To evaluate the clinical value of 6 androgens in the serum of Sj?gren's syndrome (SS) measured by liquid chromatography tandem mass spectrometry (LC-MS).Methods 32 SS patients (SS group) and 25 healthy people (healthy group) were included in this study.6 androgens in the serum were analyzed by LC-MS after prepared.PCA,PLS-DA models and t-test were used to class differentiation of androgens between two groups.Results The results of PLS-DA showed that SS group and healthy group could be well classed by 6 androgens.The levels of testerone (T),dihydrotestosterone (DHT),dehydroepiandrosterone (DHEA),androsterone and DHEAS in SS group were significantly lower than those in healthy group (t=8.536,2.438,3.172,4.158,4.489,all P<0.05).The samples before or after menopause could be distinguished between the two groups by PLS-DA.Conclusion The significant difference of androgens was discovered between SS patients and healthy people via the measurement of 6 androgens in serum.It may arise a new idea for diagnosis and treatment of SS.

Structure of a recombinant chimeric anti-CD20 IgG1 monoclonal antibody was verified by the application of high-performance liquid chromatography-mass spectrometry (HPLC-MS)and N-terminal sequencer. Molecular masses, N-terminal sequences and peptide maps of the antibody treated with different reagents and enzymes were measured. Results indicate that the amino acid sequences of light and heavy chains and 10 disulfide bonds were consistent with theoretical structure. By comparison of molecular masses and peptide maps for the fully glycosylated and deglycosylated samples, the N-linked glycosylation site was identified. The method is simple, rapid, precise, and could be referred to the quality control and structure determination of other IgG1 products.