Objective To investigate the expression and clinical signification of tumor M2 pyruvate kinase in pa-tients with CRC, and to try to find a reliable and atraumatic method to predict the progress and pronosis in the CRC. Methods ELISA method was used to determine the serum levels of tM2-PK in 68 patients with CRC,40 pa-tients with colorectal polyps and 40 cases of normal subjects; immunohistochemistry ( IHC ) methods were respec-tively used to detect the expression of tM2-PK in tissues. Results ① The level of serum tM2-PK in patients with CRC was significantly higher than the group of patients with polyps and the normal subjects ( P<0.05 ) . The serum tM2-PK levels was closely correlated to tumor size and Duke stage.②The positive rate of tissue tM2-PK was signif-icantly higher in CRC than in colorectal polyps and normal subjects ( P<0.05 ) . Tissue tM2-PK levels was closely correlated to tumor size,Duke stage and differentiation grade. ③Serum tM2-PK levels was correlated to tissue tM2-PK levels (r=0.357,P=0.003). Conclusion Combined detection of expression of tM2-PK can provide the basis theory for the diagnosis and prognosis evaluation of CRC.

With the progress of genetic studies,it has been found that long non-coding RNA( LncRNA) in many regulations which play a key role in development of tumor.Its own individual pieces can also have differ-ent adjustive effects;some LncRNA can even have different regulation and controlling effect in a variety of tumor. How the newly discovered LncRNA expression in colorectal cancer?This review mainly outlines the research de-velopment of LncRNA in colerectal cancer in brief.

At present,the main treatment methods of solid tumor are radiotherapy and ehemotherapy.Traditional radiotherapy and chemotherapy have certain limitations because of resistance and toxicity.High dose chemoradiotherpy with hematopoietie stem cell transplantation(HSCT)support can reduce resistance and toxicity,enhance the effectiveness of treatment.But there are still high relapse rate after transplantation and short survival period.How to strengthen the following treatment is worth of further study.This review will focus on the feasibility,treatment time,therapeutic effect and research advancement of HSCT for solid tumor.

AIM: To establish the quality standard for Duhuo Jisheng Mixture (Radix Angelicae Pubescentis, Herba Taxilli, Cortex Fraxini, etc.) METHODS: Herbs in Duhuo Jisheng Mixture were identified by TLC, and osthol content was determined by TLCS. RESULTS: Rhizoma Chuanxiong; Radix Angelicae Sinensis; Radix Saposhnikoviae; Radix Gentianae Macrophyllae; Cortex Eucommiae; Herba Asari; Radix Paeoniae Alba could be determined. Osthol showed a good linear relationship at a range of 0.466 - 3.600 ?g,r= 0.999 2 . The average recovery was 95.4% . CONCLUSION:The methods are available with a reproducibility and can control the quality of this mixture.

AIM:To study the detection limits of aristolochic acid in Herba Asari preparation by HPLC. METHODS: Whether Asarum hetertropoides Fr.Schmidt ver.Mandshuricum(Maxim.) Kitag contained aristolochic acid A or not by HPLC and MS,and the difference in the reference substance and the sample solution was compared. RESULTS: Aristolochic acid A could not be found in A.hetertropoides Fr.Schmidt ver.Mandshuricum(Maxim.)Kitag by HPLC,but be found by MS. CONCLUSION:A.hetertropoides Fr.Schmidt ver.Mandshuricum (Maxim.)Kitag contained trace aristolochic acid,but it is difficult to measure aristolochic acid in Herba Asari by HPLC.The baseline noise of the sample solution can interfere with the detection limit of the trace compound compound

The immune nutrients play an important role in nutritional support,immune regulation and maintenance of intestinal barrier function.In recent years,with the deepening study,the effect of immune nutrients in the comprehensive cancer treatment has constantly being recognized.Many studies have shown that glutamine (Gln),arginine (Arg) and polyunsaturated fatty acids (PUFA) have effect on the tumor itself and the combination cancer chemotherapy.

Epistaxis ; diagnosis ; etiology ; Hemophilia B ; complications ; diagnosis ; Humans ; Male ; Young Adult

Objective: To study the floating and sustained release performance of Xiaojin Cinnabar Floated Sustained Release Capsules. Methods: HPMC was used to form the framwork of floated sustained release capsules containing Xiaojin Cinnabar. The content, floating time in vitro and releasing rate were studied.Results: The floating time in vitro was over 3 hours. The total released amount in 3 hours came to 60%. The conventional Xiaojin Cinnabar didn't have the floating performance. Conclusion: Xiaojin Cinnabar Floated Sustained Relegse Capsules is superior to the conventional Xiaojin cinnabar in the sustained release performance.

OBJECTIVETo compare the efficacy differences in the treatment of somatoform pain disorder between resuscitation and tranquilization acupuncture technique and oral administration of deanxit.

METHODSForty cases of somatoform pain disorder (complicated with mild and moderate depression) were randomized into an acupuncture group and a western medication group, 20 cases in each one. In the acupuncture group. acupuncture was applied to Shenting (GV 24). Shuigou (V 26). Sishencong (EX HN I) to Baihui ((V 20) (penetrating technique). Feng-chi (GB 20). etc. The treatment was given once every day. 6 times a week. In the western medication group. the anti depression drug. deanxit was prescribed for oral administration, 1 tablet in every morning and lunchtime each day. In 8 weeks of continuous treatment, the visual analogue scale (VAS) and Hamilton depression scale 17 items (HAMD) were used to evaluate the efficacy in the patients of the two groups, the adverse reactions were observed and the recurrence rate was followed up in 3 months.

RESULTSAfter treatment. VAS and HIIAMD scores were all apparently reduced as compared with those before treatment in the patients of the two groups (all P<0.05). The reducing amplitude of VAS score in the acupuncture group was greater than that in the western medication group (P<0.05) and that of HAM) score was not different significantly as compared with that in the western mnedica tion group (P > 0.05). There was no adverse reaction in the acupuncture group and were 3 cases as dizziness, trembling. dry mouth and constipation in the western medication group. The curative and remarkably effective rate was 50.0 (10(20)) in the acupuncture group, better than 15.0% (3/20) in the western medication group (P<0.05). The recurrence rate was 10.0 (2/20) in the acupuncture group. lower than 35.0% (7/20) in the western medication group (P<0.05).

CONCLUSIONThe resuscitation and tranquilization acupuncture technique achieves the definite efficacy on somatoform pain disorder, relieves depression symptoms of patients, especially pain and reduces adverse reactions and recurrence rate. The effects of this therapy are advanced as compared with deanxit.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Combined Modality Therapy ; Female ; Humans ; Male ; Middle Aged ; Resuscitation ; Somatoform Disorders ; therapy ; Treatment Outcome

Since the publication of a high-throughput DNA sequencing technology based on PCR reaction was carried out in oil emulsions in 2005, high-throughput DNA sequencing platforms have been evolved to a robust technology in sequencing genomes and diverse DNA libraries. Antibody libraries with vast numbers of members currently serve as a foundation of discovering novel antibody drugs, and high-throughput DNA sequencing technology makes it possible to rapidly identify functional antibody variants with desired properties. Herein we present a review of current applications of high-throughput DNA sequencing technology in the analysis of antibody library diversity, sequencing of CDR3 regions, identification of potent antibodies based on sequence frequency, discovery of functional genes, and combination with various display technologies, so as to provide an alternative approach of discovery and development of antibody drugs.