2.Expression,Purification and Antibody Preparation of Recombinant C8orf32 Protein
Lei ZHU ; Zheng-Xi ZHANG ; Guo-Xin NI ; Xue-Min XU ; Biao-Yang LIN ; Wei LI ;
China Biotechnology 2006;0(04):-
C8orf32 is a gene which has not been functionally characterized,the mRNA level of this gene is significantly higher in breast cancer tissues than that in normal breast tissues.The amplified cDNA fragment was inserted into the pGEX-6P1 vector fused with the upstream GST gene.The expression vector was transformed into the E.coli BL21(DE3) strain and expression of GST-C8orf32 fusion protein was induced by IPTG..After removal of GST tag by site-specific protease,the C8orf32 protein fused with an eight amino acid peptide tag was obtained.The purity of recombinant C8orf32 protein was about 95%.The identity of the purified protein was confirmed by N-terminal sequencing and tandem mass spectrometry.The polyclonal antibody was prepared by immunizing the New Zealand white rabbits with C8orf32 protein.The polyclonal antibody was proved to recognize the C8orf32 protein correctly.The purified C8orf32 protein can be used for structural and functional studies and the polyclonal antibody can be used for tissue specific protein expression profiling.
3.Assessment of Exposure to PM2.5,CO and O_3 for Elders in a Community in Winter in Beijing Using Microenvironment Measurements and Time-Activity Data
Shao-Wei WU ; Fu-Rong DENG ; Xin-Biao GUO ; Al ET ;
Journal of Environment and Health 2007;0(09):-
Objective To estimate the exposure level of PM2.5,CO and O_3 in the elders in a community in Beijing.Methods The concentrations of PM2.5,CO and O_3 in 10 main activity sites of the elders were measured and 24 h time-activity data of 30 elders was collected by recording of activity log paper,Nov.28,2007—Jan.17,2008.Results The 24 h average exposure concentrations of PM2.5,CO and O_3 for the elders were(146.54?6.60)?g/m~3,(2.67?0.18)mg/m~3 and(32.30?2.79)?g/m~3, respectively,there was no significant difference in the exposure level of PM2.5 and O_3 between male and female elders except CO (P
4.Challenge and exploration of army medical college graduate education in the context of internationalization
Shu-hui LIANG ; Li WANG ; Yong-guo ZHANG ; Bin FENG ; Xin WANG ; Biao-luo WANG ; Xue-gang GUO ; Kai-chun WU ; Jie DING
Chinese Journal of Medical Education Research 2011;10(10):1184-1186
In the context of internationalization of education,the military graduate education concepts and models also come to change,and opportunities and challenges coexist.In this article,the challenges and problems of army medical college graduate education were mentioned and analyzed,and the exploration and attempt of graduate education in the process of international were summarized.
5.The clinical significance of serum level of soluble intercellular adhesion molecule-1 detected before and after lung transplantation
Hua-long, XIAO ; Guo-qiang, XIE ; Guo-qian, CHEN ; Xiao-feng, ZHANG ; Xin-chen, QIANG ; Jing-feng, YU ; Lan, ZHU ; Bing-fang, ZHU ; Biao, HUANG
Chinese Journal of Nuclear Medicine 2010;30(5):343-345
Objective To evaluate serum soluble intercellular adhesion molecule-1 (sICAM-1) before and after lung transplantation for diagnosing acute rejection. Methods Biotin-streptavidin time resolved fluoroimmunoassay (BSA-TRFIA) was used to detect the concentration of serum sICAM-1 before and after lung transplantation in 26 patients. All patients were divided into stable lung transplantation group (n =16), acute rejection group (n =4) and infected group (n =6). The serum level of sICAM-1 in those groups was compared with that of the control group ( n = 30 ) by the non-parametric rank sum test ( KruskalWallis H test). Results No significant difference was found for serum sICAM-1 among the three groups and the control group before operation: (357.07 ± 220.01 ), ( 396. 18 ± 136.25 ), (468.95 ± 85.48 ) μg/L vs(348.63 ±69. 12) μg/L, H=6. 0436, P >0.05. However, when rejection and infection happened after operation, the serum sICAM-1 increased in the acute rejection group (455.53 ± 126.51 μg/L) and decreased in the infection group (146.43 ± 327.11 μg/L), and the level in the stable transplantation group was (274.23 ± 157.53 ) μg/L (H = 21. 8994, P < 0.01 ). Conclusion Serum sICAM-1 level might be a potential marker to differentiate acute rejection from infection after lung transplantation.
6.A pilot study of the relationship between the drainage cannula and the cerebral angioarchitecture in patients receiving stereotactic cannula placement for hypertensive intracerebral hemorrhage
Jin-Biao LUO ; Shu-Jie SUN ; Wei QUAN ; Zhi-Kai CAO ; Biao PENG ; Qi XIE ; Xin-Hua WEI ; Jian-Ping L(U) ; Guo-Cai XIAO
Chinese Journal of Neuromedicine 2008;7(10):1054-1056
Objective To investigate the clinical outcome of patients receiving stereotacticcannula placement for hypertensive intracerebral hematoma drainage and the relationship between thedrainage cannula and the cerebral angioarchitecture. Methods Sixty-three patients with hypertensiveintracerebral hematoma underwent operations for stereotactic placement of a soft tube for hematomadrainage. CT angiography and CT venography were performed prior to cannula withdrawal after thepatients' condition was stabilized or complete hematoma drainage. The relationship between the drainagecarmula, cerebral angioarchitecture and the entry route of the cannula were observed. ResultsPostoperative CT angiography and CT venography showed that the entry route of the cannula allowedsafe passage of the cannula along the cerebral arteries and veins, and the position of the cannula wasaccurate in all the patients. Satisfactory hematoma drainage and good postoperative recovery wasachieved in all the patients, and no significant injuries to the adjacent cerebral arteries or veins occurredin these cases. Conclusion Stereotactic cannula placement with the minimally invasive technique forhemotoma drainage causes minimal injury and is safe, effective, cost-effective and convenient fortreatment of hypertensive intracerebral hematoma.
7.Screening and Bioinformatics Analysis of Idiopathic Pulmonary Fibrosis Related Genes
Jing XING ; yan Xin HUANG ; biao Yu GUO
Journal of Sun Yat-sen University(Medical Sciences) 2017;38(6):926-930,937
[Objective]We aimed to explore the pathogenic genes of Idiopathic pulmonary fibrosis (IPF) by bioinformatics analy?sis and provide a target for further research.[Methods]Gene data sets GSE53845, GSE24206, GSE10667 were downloaded from the Gene Expression Omnibus database and the differential expression genes of normal tissue and IPF were screened with GEO 2R analysis tool. GO analysis and KEGG pathway enrichment analysis of differentially expressed genes were performed in DAVID database in or?der to find out the biological function and its focused signal pathway in differentially expressed genes during IPF development. In order to study the relationship between differential genes and proteins, STRING and CYTOSCAPE software were used to construct the pro?tein interaction network and MCODE software was used to extract the sub-network modules in the protein-interacting network.[Re?sults]This study found 110 differentially expressed genes, of which 92 were high expression in IPF and 18 were low expression. GO enrichment analysis showed that the up-regulated genes in IPF mainly affected the biological processes such as cell adhesion, bio-ad?hesion and collagen metabolism. The enriched molecular function was mainly involved in the composition of extracellular matrix struc?ture and the binding of calcium ions. The down-regulated proteins are mainly involved in the sensory regulation of the biological pro?cess in IPF. KEGG pathway analysis showed that the up-regulated genes in IPF were mainly involved in receptor interactions, cell ad?hesion and other signaling pathways.[Conclusions]This study uses bioinformatics to screen out the differential genes, some of which have been shown to be involved in IPF, and some genes have not been studied, suggesting that it may be a new research target for IPF pathogenesis.
8.The comparison of the indoor environmental factors associated with asthma and related allergies among school-child between urban and suburban areas in Beijing.
Hai-bo LÜ ; Fu-rong DENG ; Ji-dong SUN ; Shao-wei WU ; Xiu-ming SUN ; Xin WANG ; Yan-hua ZHAO ; Xin-biao GUO
Chinese Journal of Preventive Medicine 2010;44(7):626-630
OBJECTIVETo study the indoor environmental factors associated with the prevalence of asthma and related allergies among school children.
METHODSA cluster sampling method was used and the ISAAC questionnaire was conducted. A total of 4612 elementary students under Grade Five of 7 schools were enrolled in the survey for the impact of indoor environmental factors on the prevalence of asthma and related allergies in several urban and suburban schools of Beijing.
RESULTSA total of 4060 sample were finally analyzed including 1992 urban and 2068 suburban. The prevalence of wheeze, allergic rhinoconjunctivitis and atopic eczema in the past 12 months was 3.1% (61/1992), 5.3% (106/1992), 1.1% (22/1992) among urban children while 1.3% (27/2068), 3.1% (65/2068), 1.0% (22/2068) among suburban children respectively. The prevalence of wheeze and allergic rhinoconjunctivitis of the past 12 months in urban were both significantly higher than that in suburban (χ(2) = 14.77, 11.93, P < 0.01). The incidences of having asthma and eczema ever among urban children (5.3% (105/1992), 29.4% (586/1992)) were significantly (χ(2) = 39.03, 147.22, P < 0.01) higher than that among suburban (1.7% (35/2068), 13.8% (285/2068)). Although the distributions of indoor environmental factors were similar in both areas, passive smoking and interior decoration had different influence on the prevalence of asthma and related allergies among school children in the two areas. The significant impact of passive smoking on having asthma ever among suburban children was observed (OR = 2.70, 95%CI = 1.17 - 6.23) while no significant result in urban (OR = 1.06, 95%CI = 0.71 - 1.58); the percentage of interior decoration was 84.0% (1673/1992) among urban children and 80.0% (1655/2068) among suburban children, there was significant impact of interior decoration on the prevalence of having eczema ever among urban children (OR = 1.57, 95%CI = 1.17 - 2.10) but no significant results were found in suburban sample (OR = 1.06, 95%CI = 0.76 - 1.48).
CONCLUSIONThe prevalence of asthma and related allergies among school children is much higher in urban areas than that in suburban areas and the indoor environmental factors such as passive smoking and interior decoration may differently explain the prevalence of asthma and related allergies in the two areas.
Adolescent ; Air Pollution, Indoor ; analysis ; Asthma ; epidemiology ; etiology ; Child ; Child, Preschool ; China ; epidemiology ; Cities ; Environmental Exposure ; Female ; Humans ; Hypersensitivity ; epidemiology ; etiology ; Male ; Prevalence ; Risk Factors ; Students ; Suburban Population ; Surveys and Questionnaires ; Urban Population
9.Pleiotropic effect of tatC mutation on metabolism of pathogen Yersinia enterocolitica.
Zhi-Yang SHI ; Hua WANG ; Ling GU ; Zhi-Gang CUI ; Long-Fei WU ; Biao KAN ; Bo PANG ; Xin WANG ; Jian-Guo XU ; Huai-Qi JING
Biomedical and Environmental Sciences 2007;20(6):445-449
OBJECTIVETo analyze the impact of depletion of the twin arginine translocation (TAT) system on virulence and physiology of Yersinia enterocolitica for a better understanding of its pathogenicity.
METHODSWe constructed a DeltatatC::SpR mutant of Yersinia enterocolitica by P1 phage mediated transduction using Escherichia coli K-12 DeltatatC::SpR strain as a donor.
RESULTSA P1-mediated genetic material transfer was found between the two species of enterobacteria, indicating a great potential of acquisition of antibiotic resistance in emergency of a new threatening pathogen by genetic material exchanges. Periplasmic trimethylamine N-oxidase reductase activity was detected in the wild type Y. enterocolitica strain and translocation of this enzyme was completely abolished by the DeltatatC::SpR mutation. In addition, the DeltatatC::SpR mutation showed a pleiotropic effect on the metabolism of Y. enterocolitica. However, the tat mutation did not seem to affect the mobility and virulence of Y. enterocolitica under the conditions used.
CONCLUSIONUnlike other pathogenic bacteria studied, the TAT system of Y. enterocolitica might play an important role in the pathogenic process, which is distinct from other pathogens, such as Pseudomonas aeruginosa and enterohemorrhagic E. coli O157:H7.
Drug Resistance, Microbial ; genetics ; Genes, Bacterial ; Mutation ; Oxidoreductases Acting on CH-NH Group Donors ; metabolism ; Transduction, Genetic ; Virulence ; Yersinia enterocolitica ; enzymology ; genetics ; metabolism ; pathogenicity
10.The design of inferior rectus abdominis flap and its study of applied anatomy.
Wei-hong WANG ; Zhi-rong ZOU ; Zong-liang LIU ; Xiang-xin MOU ; Guo-mim LI ; Biao XU
West China Journal of Stomatology 2010;28(3):282-285
OBJECTIVETo describe the anatomy of inferior epigastric artery and its correlation with rectus abdominis muscle in order to provide anatomical basis for reconstruction of defect in the oral and maxillofacial region.
METHODS10 Chinese adult specimens fixed by 10% formalin were simulated for the design of inferior rectus abdominis flap, and then observing, taking a photograph during the cadavers were being operated and measuring by image analysis software in the end.
RESULTSThe original external diameters of inferior epigastric artery and length of its trunk were (2.58 +/- 0.60) mm and (85.87 +/- 14.90) mm respectively. Its perforators distribute intensively toward hilum.
CONCLUSIONWhether rectus abdominis myocutaneous flap or deep inferior epigastric perforator flap adapts to reconstruct the defect of oral and maxillofacial region due to invariable traveling of inferior epigastric artery and its wide original external diameters relatively.
Adult ; Cadaver ; Epigastric Arteries ; Humans ; Reconstructive Surgical Procedures ; Rectus Abdominis ; Surgical Flaps