10 cm respectively from the tumor),the metastatic rate of lymph nodes was 34.7%,2.5% and 1.7%,respectively.The metastatic rate of LN in right colon was significantly higher than that in left colon(52.0% vs 33.8%,P

Objective To discuss the safety management of hemodialysis equipment for safe and effective treatment to patients.Methods The management methods were summarized in aspects of the installation,utilization and maintenance of hemodialysis equipment.Results The hemodialysis patients received the safe and effective treatment through the strict management and the standardized system.Conclusion The standardized management can not only reduce the unnecessary failures of the equipment,and prolong the service life,but also increase the utilization rate of the equipment,and provide the best,safest and most satisfactory treatment for patients.

Objective To investigate effects of cerebral isehemic preconditioning on neurological function,in- farct volume,and the expression of caspase-3 in brain issue after brain ischemia again,and to investigate the brain protection mechanism produced by cerebral ischemia preconditioning.Methods 48 healthy male Sprague-Dawley (SD)rats(weighted 200～250g)were randomly divided into 2 groups:ischemia group(n=24),ischemia precondi- tioning group(namely preconditioning group,n=24).Each group was divided into 4 subgroups according to 6h,1d, 2d,4d after ischemia again.Results At the same point of time after ischemia,neurological deficit in preconditioning group was much less than that in ischemia group,the difference was significant(P

Objective To measure and analyze the staffing standards of family doctor interdisciplinary team by means of the WHO workload indicator of staffing need (WISN)method,for reference of the government in building family doctor interdisciplinary teams.Methods 150 community health centers in 16 provinces were selected.The related data from 150 centers were collected to analyze the population served by each family doctor,and the staffing standards of family doctor interdisciplinary team by means of the WHO workload indicator of staffing need(WISN)method.Results There were 10 721 community health professionals in the sample centers which provide 132.14 million standard equivalents of service to the public.1 9 6 1 6 community health professionals were needed to ensure the quality of service and no extra workload after work for professionals.Averagely,each family doctor can serve 1 558 residents,who needs 1.3~1.5 nurses or public health workers.Conclusions A huge gap was found for community health professionals.The population served by each family doctor should be lowered compared to the governmental requirement and more nurses should be introduced to the family doctor interdisciplinary team.

Objective:To investigate effects of depside salt from salvia miltiorrhiza(DSSM)on the dental pulp of rats under repeated exposure to +Gz acceleration.Methods:27 male SD rats were randomly divided into 9 groups(n =3).The rats in negative control group was only fixed with rats kept-keeling device,in positive control group were exposed to +5 Gz and +10 Gz respectively for 30 s each time,5 times per day,4 days per week,for 3 consecutive weeks.The rats in other groups were respectively given DSSMat 2,6 and 18 mg/kg respectively 30 min before each +Gz exposure.The rats in negative and positive control groups were injected with 0.9%sodium chloride solution 30 min prior to +Gz exposure.After the experiment,The pulp changes were observed by HE staining.Real time qPCR was applied to determine the relative expression of heat sheck protein(HSP70)mRNA.Results:+Gz exposure decreased the activity degree of the rats,and resulted in damage of rat dental pulp.With the increase of +Gz value,the relative expression of HSP70 mRNA was increased(P ＜0.05).HSP70 mRNA expression in drug groups was less than that in their own positive control groups,but showed statistical significance only in the 18 mg/kg and +10 Gz group(P ＜0.05).Conclusion:+Gz exposure may dam-age dental pulp.DSSMhas certain protective effect under +Gz exposure.

Carcinoma, Hepatocellular ; pathology ; Diagnosis, Differential ; Humans ; Liver Cirrhosis ; pathology ; Liver Neoplasms ; pathology ; Precancerous Conditions ; pathology

AIM:To observe the treatment for ptosis patients with severe undercorrection after frontal muscle flap suspension surgery. · METHODS: Totally 46 patients ( 67 eyes ) were randomly divided into 2 groups: 23 cases ( 33 eyes ) in Group A were given conjoint fascial sheath ( CFS ) suspension surgery; 23 cases ( 34 eyes ) in Group B Whitnall ligament suspension. Postoperative follow-up was 6mo. Corrected rate, recurrence rate and postoperative adverse reactions were compared and analyzed. ·RESULTS:The corrected rates of the two groups were both satisfied without significant difference between the two groups (P>0. 05). the Whitnall ligament suspension surgery had less recurrence than CFS suspension surgery, but the difference was not statistically significant (P>0. 05). The rate of hypophasis after CFS suspension surgery was significantly less than that after Whitnall ligament suspension (P<0. 05). ·CONCLUSION:The two operations both have satisfied corrected rate and low recurrence rate, the Whitnall ligament suspension surgery is less damaging and more easy to operate, while CFS suspension surgery is repeatable and less rate of hypophasis.

Objective To investigate the clinical effect of ultrasound-guided sclerosing therapy with injection of lauromacrogol for the treatment of pelvic encapsulated hydrops. Methods A total of 40 patients with pelvic encapsulated hydrops were enrolled in this study. Under ultrasonographic guidance , puncture aspiration of pelvic encapsulated hydrops was carried out in all patients , which was followed by lauromacrogol injection of appropriate dose, and the drainage catheter was kept in the hydrops cavity. The clinical results were evaluated at one, 2 and 3 months after the treatment. Results The success rate of puncturing was 100%. Six months after the treatment, the complete cure was obtained in 86.4%of patients, and the effective rate was 100%. Conclusion For the treatment of pelvic encapsulated hydrops , ultrasound-guided sclerosing therapy with injection of lauromacrogol is effective and safe with instant result and lower recurrence rate. Therefore, this technique should be recommended in clinical practice.

Objectives To analysis the expression difference of eIF4E in bone marrow between the acute myeloid leukemia (AML) and the non-tumor patient,before and after induction therapy,and the different subtypes of AML,and to explore the relation between eIF4E and other molecular biology abnormalities in AML.Methods The bone marrow specimens of newly diagnosed AML and non-tumor control patients were collected.The expression level of eIF4E was detected by RT-PCR.Results The positive rate of eIF4E was 65.2 ％ (101/155) in AML.eIF4E turned to negative in 12 patients (17.6 ％) who got complete remission after induction therapy.eIF4E was negatively expressed in bone marrow of the nontumor control patients.The positive rates of eIF4E were 75.0 ％ (15/20) and 80.8 ％ (21/26) in M4 and M5 type AML,respectively,and was 59.6 ％ (65/109) in other subtype AML (P ＞ 0.05).FLT3/ITD gene mutation was found in 26 cases of newly diagnosed AML.The eIF4E expression and FLT3-ITD gene mutation were independent each other (P ＞ 0.05).Conclusions eIF4E is positive in most of the newly diagnosed AML and turns to negative in part of AML achieving complete remission.The expression of eIF4E is no difference among different subtype of AMLs.eIF4E and FLT3-ITD gene mutation are independent each other.

Objective To observe the intervention effect of Ginkgo biloba extract EGb761 on tert-butyl hydroperoxide ( t-BHP)-induced injury in human umbilical cord-derived mesenchymal stem cells ( hUC-MSCs) . Methods Proliferation of hUC-MSCs after primary culture was detected by methyl thiazolyl tetrazolium (MTT) assay, and then the optimal concentrations of t-BHP and EGb761 for oxidative stress injured MSC model were screened. Cell apoptosis was assessed by flow cytometry after Annexin V-fluorescein isothiocyanatel propidium iodide ( Annexin V-FITC/PI) staining. Content of malondialdehyde ( MDA) and superoxide dismutase (SOD) activity in hUC-MSCs were evaluated, and the expression levels of p53 and p21Cip1/Waf1 were analyzed by real-time fluorescence PCR. Results Pretreatment with 10~200 mg/L of EGb761 for 3 hours reduced the sensitivity of hUC-MSCs to t-BHP ( 100 μmol/L) induced proliferation inhibition, while EGb761 over 100 mg/L had no significant effect on enhancing the protection of hUC-MSCs . EGb761 at 100 mg/L prohibited hUC-MSCs apoptosis and MDA accumulation in hUC-MSCs induced by 100μmol/L of t-BHP acting for 6 hours, maintained the enzymatic activity of SOD, and decreased the expression of p53 and p21Cip1/Waf1 in hUC-MSCs with t-BHP-induced injury. Conclusion EGb761 is capable of protecting hUC-MSCs against oxidative stress injury, and its mechanism is probably related with the modulation of p53/p21 signal pathway.