Objective To investigate the effect of amyloid on the expression of kinesin superfamily(KIFs) genes in cortex of Wistar rats. Methods The animal model of Alzheimer's disease was prepared by injection of amyloid into brain ventricle of rats.The learning impairments were verified by water maze test.The levels of mRNA for six kinesin superfamily genes,kinesin,KIFlb,KIFlc,KIF3a,KIF3c,and KIF4,in cortex of rats,were estimated by real-time RT-PCR. Results Two weeks after amyloid injection,the rats showed significant learning impairments.In cortex,the expression of KIF genes was increased after amyloid injection.Conclusion\ The increase of KIF gene may be an evidence for the excitotoxity of amyloid to neurons.

Objective To investigate the moderator of children' s perceptions quality of parental involvement on parenting style and children' s sport expectancy-value beliefs.Methods 595 students of grade 2 to 6 from five elementary schools were chosen and analyzed.Data were analyzed by SPSS16.0 software packet using independent sample t test,One-Way ANOVA,correlation analysis and hierarchical regression statistical analysis of regulation.Results Children' s expectancy beliefs was predicted by authoritarian and behavior encouragement(β=0.076,t =2.35,P=0.019 ;β =0.111,t =3.748,P =0.000).Children' s value beliefs was predicted by affect -warmed and behavior encouragement (β =0.070,t =2.883,P =0.004 ;β =0.070,t =2.745,P =0.006).Children' s sport behavior was predicted by trust-encouragement(β=0.294,t=3.193,P=0.001).The prediction of expectancy beliefs was significant positive by the interaction between indulgence and time involvement (β=0.078,t =2.065,P =0.039).The prediction of expectancy beliefs was significant negative by the interaction between authoritarian and time involvement(β=-0.084,t =-2.454,P=0.014).The prediction of expectancy beliefs was borderline significant positive by the interaction between neglecting and equipment provision(β=0.073,t =1.94,P =0.053).Conclusion Different parenting styles are significant predicators of children sport involvement.Children' s perceptions quality of parental involvement play an important role on parenting style and children' s sport expectancy-value beliefs.

The human sphincter of Oddi has been known for more than a century.The sphincter regulates periodic discharge of bile and pancreatic juice and their directional flow.Research on the sphincter is still inadequate,especially on how to accurately evaluate its function,dysfunction and effective treatment.In recent years,advances in anatomy,regulation and improvements in investigation have deepened our understanding of diagnosis and treatment of sphincter of Oddi dysfunction. This paper summarizes the new developments in this field.

The development of clinical microbiology laboratory is an essential part in promoting the development of clinical microbiology and the basis rational use of antimicrobial agents.Under the circumstances of national specific rectification of antimicrobials,clinical microbiology laboratory should focus on self-buildup and improvement.The development of clinical microbiology laboratory should focus on development of both software and hardware capabilities for lab examination and diagnosis in an all-round way,enhance the antimicrobial susceptibility surveillance,and emphasize and communication and cooperation between laboratory and other departments e.g.clinical departments,pharmacy department and nosocomial surveillance and control office,making concept translation from past ‘paramedical department' to a ‘platform department',contribute its due share to the rational uses of antimicrobial agents.

Objective To explore the expression of cell apoptosis regulatory elements in esophageal canceration course. Methods 60 specimens of esophageal carcinoma and corresponding adjacent no cancerous tissue were detected quantitatively by propidium iodide(PI) stain and indirect immunofluorescence of fluorescein isothiocyanate(FITC) using flow cytometry(FCM) equipment. Results Expression of bcl-2 and c-FLIP in esophageal carcinoma was significantly higher, and Fadd, Caspase-8 and Caspase-3 were significantly lower than that in adjacent tissues (P <0.01). There was positive correlation between Fadd and Caspase-8 gene (P <0.01 or P <0.05). The expression of Fadd was positively correlated to Caspase-8. Comparing with normal epithelium and dysplasia tissue, DNA content and heteroploid cells were increased significantly in esophageal carcinoma. Conclusion During the esophageal canceration course, expressions of relevant cell apoptosis regulatory elements were abnormal. DNA content and heteroploid cells were increased significantly in esophageal carcinoma.

Objective To investigate the authors, the institution and endemic distribution of these authors of Chinese Journal of Orthopaedics, and understand the current situation and problems in the field of Orthopaedics. Methods The authors, the co-authors and the core authors of papers published in Chinese Journal of Orthopedics from 2005 to 2009 were quantitatively analyzed using literature search through CMCI.The distribution of institution, region and highly quantitative organization were also analyzed. Results The 1182 papers were published by the Journal within 5 years. There were 1108 papers with one or more co-authors; the cooperative rate and degree were 93.74% and 5.02, respectively. 74 papers were written by single author, which accounted for 6.26% of all papers. 615 authors (75.18% of all first authors) published only one paper. There were 295 (24.96%) papers from 67 (8.19%) core authors. 601 (50.85%) papers were from 20 high-productivity institutes. The top three high-productivity institutes were Tianjin hospital, Beijing Jishuitan hospital and Nanjing Drum Tower hospital of Nanjing University Medical School. The number of papers were 89 (7.53%), 75 (6.35%) and 43 (3.64%), respectively. The number of papers from the teaching hospital,provincial or municipal hospitals, military hospitals were 722 (61.08%), 226 (19.12%) and 157 (13.28%),respectively. According to the distribution of Province, Beijing was the first of place with 264 (22.34%) papers, followed by Shanghai by 160 (13.54%) papers and Tianjin by 136 (11.51%) papers. The distribution range of the authors has been expanded to five countries. Conclusion The influence of the Chinese Journal of Orthopaedics has greatly increased. Authors of the Chinese Journal of Orthopaedics have a highly cooperative rate. There is a group of active and talented core authors who has a great influence on the journal. But the number of core authors and the papers published by core authors need to be expanded.

Objective To explore the clinical effect of hemodialysis combined with hemoperfusion in treatment of secondary hyperparathyroidism. Methods 32 patients with secondary hyperparathyroidism were selected and randomly divided into two groups:the observation group(16 cases) and the control group(16 cases). The patients in the observation group were treated with hemodialysis combined with hemoperfusion, and in the control group, the patients were treated with conventional hemodialysis. Then the levels of serum phosphorus, serum calcium, serum intact parathyroid hormone(iPTH) and skin itching,and weakness and so on were compared. Results After treatment,the level of iPTH in the observation group decreased significantly(P ＜ 0.05) ,but in control group it decreased not obviously, and compared with it before treatment there was no significant difference. After treatment, in the two groups the level of serum phosphorus was significantly lower(P ＜0.05) ,and the level of serum calcium was significantly higher (P ＜ 0.05), and the level of serum phosphorus and iPTH had significant differences between the two groups (P ＜0. 05) ,while no significant difference in serum calcium. In the observation group,skin itching,muscle weakness and other symptoms were improved significantly compared with those in the control group (P ＜ 0.05). Conclusion Hemodialysis combined with hemoperfusion in treatment of secondary hyperparathyroidism was very effective.

Lipoprotein lipase (LPL) is the rate-limiting enzyme for the hydrolysis of the triglyceride core of circulating triglyceride-rich lipoproteins.LPL can not only affect the lipoprotein level,but also play an important role in the development of atherosclerosis.LPL gene polymorphisms may affect LPL activity,and therefore have both pro-and anti-atherogenic effects.This article reviews the relationship between LPL gene polymorphism and atherosclerosis.

Extensive use of tributyltin (TBT) has caused serious environmental pollution, which is harmful to human and other organisms. As a cytotoxic chemical, an important mode for TBT effects on the cells is to induce apoptosis. The present paper mainly reviewed the mechanism by which TBT induces apoptosis at low concentration.

Objective To investigate the effect of isochlorogenic acid B(ICAB)on CCl4-induced acute liver injury(ALI)in mice. Methods The animal model of CCl4-induced ALI in mice was established and then the protective effect of ICAB was evaluated using this model. Serum levels of alanine transaminase(ALT)and aspartate aminotransferase(AST),hepatic levels of malondialdehyde (MDA)and the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)were measured by spectrophotometric methods. Liver cell morphology was observed by hematoxylin and eosin staining method and the effects of ICAB on the protein expres-sion of nuclear factor erythroid-2-related factor 2(Nrf2),heme oxygenase-1(HO-1)and quinone oxidoreductase 1(NQO1)in mice he-patocyte were determined by Western blot method. Results ICAB(5,10 and 20 mg/kg)significantly protected against CCl4-induced liver injury by reducing the elevated levels of serum aminotransferases and hepatic MDA and remarkably restored the impaired antioxi-dants. Meanwhile,the histopathological changes were also attenuated in mice. In addition,ICAB could induce the protein expression of Nrf2 and promote its nuclear translocation,and further increase the protein expression of HO-1 and NQO1. Conclusion ICAB has protective effect against CCl4-induced ALI in mice,which is mainly due to its ability to promote the nuclear translocation of Nrf2 and decrease the oxidative stress.