BACKGROUND:Studies have confirmed that nicotine affects the activity of osteoblasts, osteoclasts, fibroblasts and erythrocytes.
OBJECTIVE:To study the nicotine effects on osseointegration and the expression of osteoprotegerin and bone morphogenetic protein 2 after implantation of dental implants with surface treatment by sandblasting or acid etching.
METHODS:Twenty-four Sprague-Dawley rats were randomly assigned to two groups and received daily injections for 2 weeks as folows: Nicotine 2 mg/kg twice for experimental group, saline solution for control group. Then the titanium implants with surface sandblasting or acid etching were implanted into the tibiae folowed by continuous nicotine or normal saline injection. At weeks 2 and 4 after implantation, the implants and surrounding bone tissue were prepared for CT, X-ray and hematoxylin-eosin staining examinations to evaluate bone healing and expression levels of bone-related genes were measured by quantitative RT-PCR.
RESULTS AND CONCLUSION: Compared with the control groups, the degree of osseointegration and the expression of osteoprotegerin and bone morphogenetic protein 2 in the experimental groups were decreased significantly (P < 0.05), except that the expression of bone morphogenetic protein 2 in the experimental group with acid etching was not significantly reduced. In addition, the expression of bone morphogenetic protein 2 in the experimental group with acid etching was higher than that in the experimental group with sandblasting at 2 weeks after implantation (P < 0.05). The X-ray and CT show that the quantities of new generation bone and the degree of bone mineralization of the sandblasting group were significant lower than those of the acid etching group under the intervention of nicotine. Hematoxylin-eosin staining showed that the activity and quantity of osteoblasts around the implants down-regulated significantly, but acid etching-treated implants showed better outcomes than sandblasting-treated implants.

Objective To investigate the year rhythm of laryngeal cough.Methods To record the attack months,the syndromes of TCM and degrees of symptoms in detail and analyze the data by percentage and Ridit analysis.Results The periods of laryngeal cough are in spring(March to May) and in autumn(September to November).The syndromes of TCM such as wind violating lung are commonly coughed in spring and primarily mild degree.The syndromes of TCM such as fire hyperactivity are commonly coughed in autumn and primarily moderate degree.Conclusions There is significant year rhythm in laryngeal cough.To master the rhythm can help the diagnosis and treatment.

The vaccine based on dendritic ceil has becomes a new research focus in the biotherapy of breast cancer. And the ways of making the vaccine are vary and no one approach has been shown to be superior to another.

Objective To explore the epidemiological characteristics of malaria in Liaoning Province from 1951 to 2014,so as to provide the evidence for further control of the disease. Methods The data of registered malaria cases in Liaoning Province from 1951 to 2014 were collected and analyzed with epidemiological methods. Results From 1951 to 2014,there were report-ed malaria cases each year. The peaks of incidence were in 1953,1962 and 1973,the incidence rates were 136.67/100000, 256.81/100000 and 35.89/100000 respectively and the numbers of patients were 27862,65460 and 11523,respectively. From 1977 to 2014,the incidence rates were all less than 1/100000. From 1951 to 1980,the occupations of patients were main-ly farmers,and from 1981 to 2014 were mainly workers,farmers and exported laborers. Conclusion The malaria endemic situ-ation in Liaoning Province experienced the periods from outbreaks to basically elimination,and the long-term surveillance will be the further working emphasis.

Objective To investigate the effect of repetitive transcranial magnetic stimulation (rTMS) combined with eye-acupuncture on cognitive dysfunction on cognitive dysfunction in patients with traumatic brain injury. Methods From May, 2014 to June, 2016, 90 trau-matic brain injury patients with cognitive impairment were randomly divided into eye-acupuncture group (n=30), rTMS group (n=30) and combination group (n=30), who accepted eye-acupuncture, rTMS and combination of eye-acupuncture and rTMS, repectively, for eight weeks. They were assessed with Loewenstein Occupational Therapy Cognitive Assessment (LOTCA) before and after treatment. Results There was no significant difference in scores of LOTCA among three groups before treatment (F<0.523, P>0.05). The scores of LOTCA were the best in the combination group after treatment (F>3.789, P<0.05). Conclusion The combination of eye-acupuncture and rTMS is more effective in improving cognitive function after traumatic brain injury than either alone.

Objective:To observe the expression and correlation of eIF4E and c-myc in laryngeal squamous cell carcinoma.Methods:Western blot was applied to examine the expression of eIF4E and c-myc on tumor core,transition core and tumor-free core from 36 laryngeal carcinoma patients.The results were statistically analyzed.Results:The overexpression of eIF4E and c-myc protein levels suggests an increasing tendency from tumor-free core,transition core and tumor core;and shows an significant correlation of eIF4E and c-myc expression.Conclusion:The overexpression of eIF4E and c-myc cause the laryngeal carcinoma cell malignant transformation;the correlation of eIF4E and c-myc may provide a basis for gene therapy of laryngeal carcinoma.

Objective To evaluate the value of high frequency ultrasound in the postoperative rehabilitation treatment of rotator cuff tear.MethodsEighty-seven patients suspected as rotator cuff tear were detected by ultrasound.Fifty four patients suffered from part or entire tear were gone under the knife.Twenty-seven patients were control group with the autonomic functional training,the other twenty-seven patients were rehabilitation group with the regular rehabilitation training,Clinical functional and ultrasound scoring were performed through the two groups to evaluate the effects of rehabilitation treatment.Efficacy evaluation assessments were taken one year after operation.ResultsThe clinical symptoms were consistent with the ultrasonographic results in different time after operation.The clinical functional and ultrasound scores in rehabilitation group had no statistical difference with control group when 2 and 4 weeks after operation (P ＞ 0.05),the clinical functional and ultrasound scores in rehabilitation group were better than control group when 8,12 and 16 weeks after operation( P＜0.05 or P＜0.01 ).The efficacy evaluation assessments in rehabilitation group were much better than control group(P＜0.01).ConclusionsHigh frequency ultrasound has great significance in the postoperative rehabilitation treatment of rotator cuff tear.

This was a restrospective clinical study of 32 children with fractured anantierior tooth fragments. The teeth was reattached by dual cured resin composite (Panavia F), 3M Z350 resin and 3M siglebond-2 bonding agent for at least 6-month recall examination. Only 2 of the restorations were lost, resulting in a 93.75% overall retention rate. The retention teeth exhibited good esthetics and normal function. The high retention rate in this study suggests that reattachment of fractured anantierior tooth fragments offers a convenient viable restorative option for the clinician.

Subcortical ischemic vascular disease (SIVD) is considered to be the most important and common cause of vascular cognitive impairment (VCI). If patients with subcortical ischemic vascular cognitive impairment (SIVCI) and subcortical vascular cognitive impairment (sVCI) can be found early, it is possible that vascular dementia (VaD) can be identified before occurrence and even reverse the process. Recent studies have shown that resting-state functional magnetic resonance imaging (rsfMRI) may provide the objective basis for the diagnosis of SIVCI. This article reviews the application of rsfMRI in the diagnosis of SIVCI.

OBJECTIVE:To establish a method for the dissolution determination of Dronedarone hydrochloride tablet,and eval-uate the quality consistency of its generic and original preparations. METHODS:UV spectrometry was performed on the column of 288 nm,dissolution media of Phosphate buffer solution (pH4.5),0.1 mol/L Hydrochloric acid solution [adding into 0.5% sodium dodecyl sulfate(SDS)],Phosphate buffer solution [pH6.8,adding into 0.5%SDS] and water,volume of dissolution medium was 1 000 ml,rotation speed was 75 r/min,the dissolution of generic and original preparations of Dronedarone hydrochloride tablet was detected,and the similarity of dissolution curve was evaluated by calculating the similarity factor (f2). RESULTS:The linear range of dronedarone hydrochloride was 2.147-25.764 μg/ml;RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries 4 dissolution media were 99.53%-101.05%(RSD=0.48%,n=9),98.95%-100.05%(RSD=0.39%,n=9), 99.54%-100.20%(RSD=0.24%,n=9)and 98.54%-100.06%(RSD=0.44%,n=9). In the 4 dissolution media,f2 of the dissolu-tion curve of 3 batches of generic and original preparations of Dronedarone hydrochloride tablet was 56,60,63,68,68,52,59, 67,65,68,76,62,respectively. CONCLUSIONS:The method is suitable for the dissolution determination of Dronedarone hydro-chloride tablet;meanwhile,the in vitro dissolution curves of generic and original preparations of Dronedarone hydrochloride tablet show similarity,so the quality consistency is good.