[ ABSTRACT] AIM: To study the influences of tangeretin ( TGN) on the growth and invasion of non-small-cell lung cancer ( NSCLC) cells, and to explore the molecular mechanisms.METHODS: The A549 cells were treated with different concentrations of TGN in vitro.The relative cell activity was determined by MTT assay.The apoptotic rate was an-alyzed by flow cytometry with Annexin V-FITC/PI staining.The number of the invasive cells was measured by Transwell as-say.The mRNA expression of MMP-2 and MMP-9 was detected by RT-PCR, and the protein levels of Ki67, Cyt C, caspase-3, cleaved caspase-3, MMP-2, MMP-9, Akt, p-Akt and p-PI3K were determined by Western blotting analysis. RESULTS:TGN inhibited the proliferation of A549 cells in a dose-dependent manner ( P<0.05) along with the low ex-pression level of proliferation biomarker Ki67.TGN up-regulated the protein levels of Cyt C, caspase-3 and cleaved caspase-3 (P<0.01) and promoted the apoptosis of A549 cells in a dose-dependent manner.Moreover, TGN down-regu-lated the invasion-related molecules MMP-2 and MMP-9 at the mRNA and protein levels, and the number of invasive cells reduced with the increase in the concentration of TGN.The protein levels of p-Akt and p-PI3K in the A549 cells was re-duced (P<0.05), and no difference of the cell viability in the cells treated with different concentrations of TGN was ob-served after blocking PI3K/Akt signaling pathway using LY294002.CONCLUSION: TGN inhibits the growth and inva-sion of A549 cells and promotes the cell apoptosis by potentially inhibiting PI3K/Akt signaling pathway activation.There-fore, this study will provide a new target for the prevention and control of NSCLC.

In recent years, monoclonal antibody comes to be a new and promising kind of treatment mode for malignant lymphoma, because of its advantages, such as target property, minimal residual of tumor cell in vivo and little side effect. The main methods include single agent therapy, combination with chemothera-py, combination with immunotoxin, radioimmunotherapy and etc.

Objective: To investigate the changes of serum levels of leptin in chronic heart failure (CHF) rats with cachexia and to study its mechanism via leptin receptor expression and leptin signal transduction pathways.
Methods: There were 15/60 male SD rats were randomly used as Control group. CHF model was established in rest 45 rats by isoproterenol hydrochloride (ISO) injection as CHF group, n=36;9 rats died. According to body weight changes and echocardiography examination, CHF rats were further divided into 2 groups:cCHF group, the rats with cachexia, n=16 and ncCHF group, the rats with non-cachexia, n=20. Serum levels of leptin and protein levels of janus activating kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3), suppressor of cytokine signaling 3 (SOCS3) were examined by ELISA;the expressions of leptin receptor in the myocardial and adipose tissues were observed by immunohistochemistry;mRNA expressions of JAK2, STAT3 and SOCS3 in adipose tissue were detected by RT-PCR.
Results: Serum levels of leptin, JAK2, STAT3 and the expressions of JAK2, STAT3 in adipose tissue were higher in both ncCHF and cCHF groups than Control group, P<0.05;protein levels and mRNA expressions of JAK2 and STAT3 were similar between cCHF group and ncCHF group, P>0.05. Serum levels of leptin, SOCS3 and mRNA expression of SOCS3 in cCHF group were lower than ncCHF group, P<0.05. The expressions of leptin receptor in myocardial and adipose tissue were higher in cCHF group than ncCHF group, P<0.05, while the expressions in ncCHF group were higher than Control group, P<0.05.
Conclusions: Increased serum level of leptin was involved in HF occurrence, leptin receptor expression and JAK2/STAT3 signal transduction pathways might be related to CHF combining cachexia in experimental rats.

Objective To put forward suggestions for improving the writing standards of package inserts of Chinese patent drug in China. Methods Totally 84 package inserts of Chinese patent drug were chosen randomly. The labeled items in them would be investigated and analyzed according to legal documents in China. Results The rate of complete labeled items was 0%. Meanwhile, many problems existed in the items which affected medication safety seriously. In addition, there were defects in expression of the content, revision of package inserts of Chinese patent drug and so on. Conclusion It is urgent to improve the writing standards and the completeness of the labeled items as well as the scientificalness on the expressions of package inserts of Chinese patent drug.

Objective To study the effects of pronunciation after cochlear implantation in children,and find out the common rules of initials consonant pronunciation errors.Methods We used the cards as the pronunciation articulation test from hearing impairment children's hearing,language competency assessment standards and methods to evaluate auditory-verbal ability after cochlear implantation,then recorded and analyzed the errors of the initials consonant.Results After training for 10 months,we evaluated speech articulation of prelingually deaf children of 3 to 5 years old with cochlear implants 73.93%±12.76%.The most incorrect pronunciations were/s/,/z/,/sh/,/g/,/ch/,/j/,/k/,/l/,/c/,/zh/,/f/and/p/,which had resulted in substitution.According to the classification of pronunciation articulation,it focused on supradialectical and velar while the least in bilabial and blade-alveolar.Ac-cording to the classification of pronunciation method,nasal,lateral and unaspirated stop had low error rates.Conclu-sion Only do when we comprehend rules of cochlear implants in children pronunciation as well as study,can we solve problems and do well in language training.The most incorrect pronunciations in children with CI are supradia-lectical and velar,the language training should be performed aecordingly.

Animals ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Humans ; Idarubicin ; administration & dosage ; Leupeptins ; administration & dosage ; Neoplasms ; drug therapy ; pathology ; Neoplastic Stem Cells ; drug effects ; metabolism ; pathology ; physiology

Angiogenesis is an important feature of malignant neoplasm and plays an essential role in the genesis,growth and metastasis of tumors. CT perfusion imaging (CTPI),as one of the functional imaging techniques reflecting neovascularity of neoplasm,perfectly combines anatomical imaging with physiological information and can be used in a range of clinical areas,such as differentiation between benign and malignant lesions,early detection of malignancy or metastasis,prediction of tumor behavior and the assessment of responses to therapies. Many researches have been done on perfusion CT in tumors of the brain,thyroid gland,salivary gland,mammary gland,lungs,liver,esophagus,kidney,colorectum,and so on. The present article reviews the basic principles of perfusion CT and its clinical application in tumors.

Bone morphogenetic protein is a key regulation factor in osteoplast cell differentiation and bone formation.It takes a role of differentiation inducting and orientating by modulating the transcription factor and through some unknown signal transduction pathway,and its expression and function can be adjusted by signal transducting system and specific acceptor inhibiter.This article reviews the research progresses on the aspects.

Tumors are classified into two major categories,benign and malignant.One main difference between malignant and benign tumors is their tendency to metastasize and recur.The metastasis itself is an ominous sign for prognosis and responsible for most cancer deaths.The metastatic "cascade" comprises a lot of steps,including escape from the primary tumor site,penetration of local stroma,entry of local vascular or lymphatic vessels,aggregation with platelets,interaction with and adhesion to distant endothelia,extravasation,recolonization,and expansion,all the time avoiding effective immune clearance and being able to survive.It has now been shown to contain stem cell-like cells called cancer stem cells(CSC) in several solid tumors.Although generally rare,these cells appear to be highly tumorigenic and may drive tumor formation and mediate tumor metastasis.

It has been proved that severe acute respiratory syndrome (SARS) is caused by a novel coronavirus. Detecting disease in its early stage, understanding its pathways of transmission and implementing specific prevention measures for the disease are very important in the course of disease control. China has established the definitions of suspected and confirmed cases of SARS. But the laboratory tests and definitions for the diagnosis are limited.Now the primary measures for SARS include isolation, therapeutics application with ribavirin and corticosteroid, mechanical ventilation, etc. Convalescent plasma is being explored.There still are many problems to be solved in the course of conquering SARS.