Enzyme-linked immunosorbent assay for determination of thyroid-stimulating antibody in the sera of patients with Graves' disease was established using ganglioside (GLS) purified from bovine brain as binder. It was applied in clinical practice. The results showed that LATS could react with ganglioside under suitable conditions. Very significant difference was observed between normal subjects and untreated GD (P

Objective To quantitatively assess the regional myocardial function in the patients with heart failure by tissue Doppler imaging. Methods The apical 4 chamber, 2 chamber and long axis view of two-dimensional tissue Doppler imaging were acquired in 30 healthy individuals and 18 patients with heart failure by GE Vivid 7 commercially machine.The basal and middle segments in left and right ventricle were analyzed in tissue velocity imaging model. The following cardiac intervals and velocities were measured in each segment, including the isovolumetric contraction time(IVCT), the ejection time, the isovolumetric relaxation time(IVRT); the rapid filling time, the artrial systolic time, the peak velocity and acceleration of Sm, the peak velocity and acceleration of Em, and the peak velocity and acceleration of Am. Results Compared with control group, IVCT, IVRT and atrial contraction time were prolonged, the ejection time and rapid filling time were shorterned, peak velocity and acceleration of Sm, Em, and Am were reduced in heart failure group. Conclusions There were characteristic changes on tissue velocity imaging in the patients with heart failure which include the delay of IVCT and IVRT and reduction of velocity in Sm, Em, and Am.

Objective To quantitatively assess cardiac intervals with tissue Doppler imaging(TDI) and normal reference values. Methods Apical 4 chamber, 2 chamber and long axis views of two-dimensional TDI were acquired in 29 healthy individuals(using GE Vivid 7 commercially ultrasound machine). The basal and middle segments in left ventricle(anterior septal, anterior, lateral, posterior, inferior and septal wall) and in the free wall of right ventricle were analyzed with tissue velocity imaging(TVI) model.Cardiac intervals of each segment, which included the isovolumic contraction time, contraction to peak time, insovolumetric relaxation time, rapid filling time and artrial contraction time were measured using TVI in the left ventricle and free wall of the right ventricle. Results The successful rate of sample was 100% in basal segment of each wall. There were significant differences between the left and right ventricle in the isovolumetric contraction time, the systolic accelaration time, contraction to peak time, isovolumetric relaxation time, early diastolic time, diastasis and artrial contraction( P

Objective:To assess the survival-predictive value of TNM and Lugano staging systems in patients with primary gastro-intestinal lymphoma (PGL). Methods:A total of 73 patients with PGL were recruited from February 2001 to August 2013. All patients were diagnosed according to the TNM and Lugano staging systems. Five-year survival rate was used as the major clinical outcome. Sur-vival curves were plotted using the Kaplan–Meier method and analyzed with the log-rank test. The prognostic value of different vari-ables for clinical outcomes was assessed using the Cox multiple regression model. Results:The median follow-up time of surviving pa-tients was 42.4 months (range:1.3-158.6 months). The estimated 5-year overall survival rate was 77.82%. When diagnosed with the TNM system, the 5-year survival rates in stagesⅠ,Ⅱ,Ⅲ, andⅣwere 100%, 90.0%, 67.4%, and 22.2%, respectively (χ2=17.7956, P=0.0005). When staged by the Lugano system, the 5-year survival rates in stagesⅠ,Ⅱ,ⅡE , andⅣwere 100%, 100%, 70.7%, and 46.2%, respectively (χ2=15.6776, P=0.0013). Cox analysis showed that the invasion depth (T) (P=0.0181) and metastasis (M) (P=0.0031) were covariates that were prognostically significant for the overall survival. Conclusion:The TNM staging system is more ac-curate than the Lugano system in predicting the 5-year survival rate of patients with PGL.

Female ; Humans ; Hypertension, Pulmonary ; etiology ; Middle Aged ; Sjogren's Syndrome ; complications

Bronchogenic Cyst ; diagnosis ; Diagnostic Errors ; Goiter ; Goiter, Nodular ; diagnosis ; Humans ; Neck

Carcinoma, Adenoid Cystic ; diagnosis ; surgery ; Endoscopy ; Humans ; Neoplasm Recurrence, Local ; diagnosis ; Nose ; surgery ; Nose Neoplasms ; diagnosis

China ; epidemiology ; Coal Mining ; Humans ; Pneumoconiosis ; epidemiology ; Private Sector ; statistics & numerical data ; Public Sector ; statistics & numerical data ; Retrospective Studies

Contraindications ; Humans ; Lymph Node Excision ; Thyroid Neoplasms ; pathology ; prevention & control ; surgery

Cutaneous Fistula ; etiology ; surgery ; Female ; Humans ; Laryngectomy ; adverse effects ; Male ; Middle Aged ; Pharyngeal Diseases ; etiology ; surgery ; Reconstructive Surgical Procedures ; methods ; Surgical Flaps