OBJECTIVE: To observe monocyte chemoattractant protein 1 (MCP-1) changes in ischemic tissue during the process of angiogenesis induction in ischemic limbs by autologous mesenchymal stem cell (MSC) transplantation.METHODS: Twenty male Sprague-Dawley rats were randomized to 2 groups (n = 10): model and MSC transplantation. Femoral and tibial bone marrow was taken to isolate and culture MSCs by percoll density gradient method. Cells of the 3~(rd) or 4~(th) passage were used for transplantation. Severe bilateral hind limb ischemia was surgically created in each group rats. Two hours after model establishment, MSCs (1×10~(11)/L) were infused into the ischemic region of rats from the MSC transplantation group, and the model group received the same amount of phosphate buffered saline. Collateral artery formation was determined by angiographic analysis and histological assessment. CD68~+ macrophage infiltration was examined by immunohistochemistry. MCP-1 protein expression in the plasma and ischemic tissue was detected by enzyme-linked immunosorbent assay. MCP-1 mRNA expression in ischemic tissue was detected by reverse transcription-polymerase chain reaction.RESULTS: At postoperative 28 days, treatment with MSC transplantation lead to collateral vessel formation, and immunohistochemistry demonstrated that CD68~+ macrophage infiltration was lower compared with the model group. MCP-1 protein and mRNA expression in the plasma and ischemia tissue was significantly lower in the MSC transplantation group than in the model group (P < 0.05).CONCLUSION: Following MSC transplantation, MCP-1 may play an important role in ischemia-induced angiogenesis. This indicates that MCP-1 would become one possible target molecule for modulating inflammatory angiogenesis by MSC Transplantation.

Objective To evaluate the use of endovascular aortic repair(EVAR) and open aortic surgery(IAS) for treatment of abdominal aortic aneurysm.Methods A retrospective review of patients treated with EVAR or IAS between 2002 and 2007 was performed.The occurrence rate of perioperative complications,morbidity and mortality,survival rate,quality of life,and cost of treatment were compared.Results Forty-two patients(30INS,12EVAR) were treated.Operating time,the intraoperative blood loss,and intraoperative blood transfusion were less in EVAR group than in the IAS group(P

Objective To study the clinical effects of endovascular stent-graft repair for thoracic aortic aneurysm(TDA).Methods The clinical records of 32 patients with thoracic aorta dissecting aneurysm,including 26 men and 6 women,who underwent endovascular repair were retrospectively analyzed.The characteristics,location,and anatomic condition of dissecting aneurysm were evaluated using CTA before operation.Under general or local anesthesia,32patients underwent endovascular repair under dynamic DSA.For those patient,8 Talent,22 Yuhengjia(Beijing) and 2 Microinvasive(Shanghai) stent grafts were used.Results Among them 32 patients the distance between entry tear and left subclavian artery was

Objective To investigate the mechanism by which pancreatic cancer cells(PCC) acquire drug resistance against 5-FU and gemcitabine. Methods The cytotoxic effects of 5-FU and gemcitabine on PCC ( Panc-1, Mia-Paca-2 andCapan-1) were assessed by using Sulforhodamine B and the expression of anti-apoptotic genes of the Bcl-x L and mcl-1 were analyzed by RNase protection assay and Western blot. Results5-FU and gemcitabine effect cytotoxicity towards PCC. After repeated treatment with 5-FU, the IC 50 values in Capan-1 cells increased by 2.1 fold (P

Objective To investigate the role of AGE-RAGE system on intimal hyperplasia in autogenous vein graft of diabetic rats.Methods The experiments were performed on 40 male Sprague-Dawley rats which divided into two groups:diabetes group and control group.Autogenous vein graft model was established in all rats.Specimens were harvested 7 days and 14 days after surgery for histological examination,and RAGE,NF-?B p65 and VCAM-1mRNA were measured by semi-quantitative reverse transcription-PCR.Western Blotting and immunohistochemistry were used to detect the protein expression of RAGE,NF-?B p65 and VCAM-1.At the same time,the levels of serum AGE were tested.Results Compared with non-diabetic rats of control group,the intimal hyperplasia of vein graft in diabetic rats obviously increased(P

Objective To investigate the effect of di-( 2-ethylhexyl ) phthalate (DEHP) on pregnant rat and the protection of zinc.Methods Fifty rats were randomized equally into 5 groups consisting of blank (given 1 ml 0.9％ sodium chloride),corn oil (given 1 ml corn oil),zinc (given 1 ml zinc gluconate including 1.2 mg zinc),DEHP (given 50 mg · kg-1 · d-1 DEHP)and DEHP + zinc (given 50 mg· kg-1 · d-1 DEHP + zinc gluconate ).At the beginning of the experiment (about 7 d before pregnancy),the female rats were administered with corresponding drugs everyday.The pregnant rats were killed and the fetal rats were removed on 19th day.The following results in each group were recorded:the body weight and the organic weight of the female rats,the number and the weight of fetus rats and the placental weight.Results The weight and the coefficient of female rats' kidney/body,spleen/body,brain/ body,and heart/body in DEHP group compared with other groups were not statistical significance (all P ＞0.05).The coefficient of female rats' liver/body,uterus/body,and ovary/body of blank group were (4.4 ± 0.7 ) ％,( 1.26 ± 0.09 ) ％,( 0.083 ± 0.009 ) ％ respectively,corn oil group were (4.5 ± 0.6 ) ％,( 1.29 ±0.10)％,(0.084 ±0.008)％,zinc group were (4.4 ±0.4)％,( 1.26 ±0.08)％,(0.084 ± 0.009 ) ％,DEHP group were ( 5.4 ± 1.0 ) ％,( 1.11 ± 0.08 ) ％,( 0.074 ± 0.012 ) ％,and DEHP + zinc group were (4.4 ± 1.0)％,( 1.28 ± 0.10)％,(0.082 ± 0.007)％ ; in DEHP group the coefficient of female rats' liver/body,uterus/body,and ovary/body compared with other groups was statistical significance ( P ＜ 0.05 ).The fetal quantity,fetal weight and placental weight of female rats of blank group were 12.8 ± 2.7,(6.03 ±0.16) g,( 1.00 ±0.03) g respectively,corn oil group were 13.6 ±3.1,(6.07 ±0.20) g,(1.00±0.04) g,zinc group were 13.3 ±3.1,(6.16 ±0.18) g,( 1.00 ±0.05) g,DEHP group were 9.2±4.1,(4.03 ±0.09) g,(0.95 ±0.03) g,and zinc +DEHP group were 12.1 ±2.9,(6.09 ± 0.17 ) g,(0.99 ±0.03 ) g.In DEHP group the fetal quantity,fetal weight and placental weight of female rats compared with other groups were statistical significance ( P ＜ 0.05 ).Conclusion DEHP can damage female rats and fetal rats in gestation period.Zinc supplied before pregnancy can relieve the influence by DEHP.

Objective To evaluate the elimination of melasma with nonablative Q-switched Nd : YAG1 064 nm laser. Methods 23 cases of melasma were treated with Q-switched Nd :YAG 1 064 nm laser using the following parameters: spot size, 6 mm; pulse duration, 5-7 ns; frequency, 10 Hz, and flu-ence, 1.8-2. 0 J/cm2. The procedure was repeated weekly. Results The treatment was well tolerated without the use of topical anesthetic. There was slight congestion, no blister and crust after treatment. Routine clean and make-up can be done after treatment. The melasmas were obviously lighten or fully eliminated after 8-10 treatments and 52. 17 % of patients showed excellent results. Only 5 cases experienced an episode of purpura on eyelid after treatment and disappeared spontaneously without post-inflammatory hyperpigmentation and scarring. Followed up over 6 months, there was recurrence in 7 patients who were effective by additional treatment. Conclusions Nonablative Q-switched Nd :YAG 1 064 nm laser is a more ideal method to eliminate melasma because of the credible effect, safe, simple operation, and no downtime, in despite of recurrence.

Covered in the paper are concepts and contents of medical risk and emergency response technology.Based on a description of medical risk information management experiences in Japan,Sweden and the United States and the current studies of medical risk response information management,the paper proposed measures for China in medical risk information emergency technology.Recommendations made include establishing a comprehensive emergency medical risk information management system,scientific medical risk information collection,monitoring,assessment,early warning systems,as well as building an intelligent risk emergency information decision system and establishing medical risk communication channels,thereby preventing effectively the occurrence of medical risks.

Objective:To compare the volatile chemical constituents from the shell and kernel of Caesalpinia minax Hance.Methods:The volatile chemical compositions of Caesalpinia minax Hance were obtained by organic solvent-wet distillation and were analyzed by GC-MS equipped with a elastic quartz capillary column HP-5MS 5%Phenyl Methyl Siloxane(30m?0.25mm?0.25?m).The constituents were identified by their mass spectra.The relative percentage of the volatile constituents was calculated from the GC peak areas.Results:One hundred and fifteen kinds of chemical constituents in the shell of Caesalpinia minax Hance were separated and sixty-three of them,which accounts for 54.7%of total volatile constituents, were characterized.There are five kinds whose relative contents were more than 2.0%.One hundred and two kinds of chemical constituents in the kernel of Caesalpinia minax Hance were separated and fifty-one of them,which accounts for 50.0%,were characterized.There are seven kinds whose relative contents were more than 2.0%.Form the shell and kernel of Caesalpinia minax Hance there were forty kinds of chemical constituents the same.The most relative contents were Limonene(5.31%,shell)and 1-Hexanol(12.94%,kernel).Conclusion:This paper reports,for the first time,the comparison of volatile constituents from shell and kernel of Caesalpinia minax Hance.It is helpful for controlling the quality and further researching of Caesalpinia minax Hance.

Objective: To study the chemical constituents of volatile oil from Radix Flemingiae philippinensis. Methods: The oil was obtained by steam distillation. The chemical compositions were separated and identified by GC/MS. The relative contents in the oil were determined by area normalization method. Results: Thirty-nine compounds were gotten from Radix Flemingiae philippinensis. The compounds of its relative content that was more than 4% were as follows: ?-himachalene (1), ?-himachalene (2), ?-himachalene (3), ?-guaiene (4), Italicene (5). Conclusion: Volatile oil by steam distillation from Radix Flemingiae Philippinensis was separated and ideutified up to 80 percent in component analysis.