Lentiviral vectors (LV),a special member of retroviral vectors,has become hot in gene therapy research.Studies have shown that LV can make exogenous gene expressed stably in tumor cells.As a tool for transportation,LV plays a significant role in the gene therapy of digestive system,hematologic system and gynecological tumor,which provides a promising new way for neoplasms gene therapy.

Bruton tyrosine kinase (Btk) is a member of the non-receptor protein tyrosine kinases of Tec family.It plays an important role in growth,proliferation,differentiation and signal transduction of B lymphocyte.After activation of B-cell receptor (BCR) by diverse stimuli,Btk is activated through tyrosine phosphorylation.Activated Btk can stimulate several downstream signaling proteins.Btk and BCR signaling pathways play important roles in initiation and maintenance of B cell malignancies.This review focuses on the recent findings in association of Btk and B cell malignancies.

Objective To observe the clinical efficacy of Shenfuyishen Capsule in the treatment of diabetic nephropathy of yangxuxueyu type in high- altitude regions.Methods We divided 53 patients randomly into two groups:31 in the observation group and 22 in the control group.Patients in the control group were treated with conventional regime of Western Medicine.Those in treatment group were given extra treatment with Shenfuyishen Capsule.Then we tested serum creatinine (Scr ),uric acid (UA),urinary microalbumin (MALB )and GFR. Results The total efficiency was 90.32% and 81.82% in the treatment group and the control group,which was significantly different (P < 0.05 ).Major symptoms improved after treatment in both groups,which differed significantly compared with those before treatment (P <0.05).ALT in the treatment group was not different after treatment.CR,UA,MALB and GFR significantly differed before and after treatment in both groups,especially in the treatment group (P <0.05).Conclusion Shenfuyishen Capsule helps improve major clinical symptoms and decrease CR,GFR,UA and MLAB levels in patients with diabetic nephrology,thus protecting renal function and delaying the process of diabetic nephropathy.

Objective To determine the antagonism of dizocilpine maleate(MK-801) and taurine to glutamate metabolism disturbance induced by methylmercury(MeHg) in cerebrum.Methods Forty Wistar rats were randomly divided into five groups.The first group was the control group,the second one was low dose MeHg-exposed group and the third was high dose MeHg-exposed group,these three groups were given physiological saline respectively.The fourth group was subcutaneously injected with 0.3 ?mol/kg of MK-801 and the five group was subcutaneously injected with 1 mmol/kg of taurine.Two hours later,the control group was intraperitoneally given physiological saline,the second group was intraperitoneally given 4 ?mol/kg of methylmercury chloride,the third,fourth and five groups were given 12 ?mol/kg of methylmercury chloride.The administration above was given five times a week for 4 weeks.The contents of mercury and Glu and Gln and the activities of GS and PAG in pallium were determined.Results Compared with the control group,the activities of PAG and the contents of mercury and Glu in MeHg-exposed groups increased significantly,the activities of GS and the contents of Gln decreased significantly.Compared with high dose MeHg group,in MK-801 and taurine treated groups,no significant differences were seen in the contents of mercury,and the contents of Glu,the activities of PAG were decreased significantly,the contents of Gln and the activities of GS were increased significantly.Conclusion Methylmercury can disorder glutamate metabolism in cerebrum of rats,and MK-801 and taurine can effectively counteract the adverse effects of methylmercury.

Objective To observe the adverse effect of methylmercury on the cortex and cerebellum respectively as well as to approach the antagonisms of dextromethorphan(DM) and riluzole to the mercury-induced neurotoxicity.Methods Twenty-eight Wistar rats were randomly divided into 4 groups by weight,7 in each.The first group was the control group and the second group was methylmercuric chloride(MMC) group.The third and fourth groups were DM and riluzole group respectively.Both of the former groups were subcutaneously injected with saline.The third and fourth groups were subcutaneously injected with 13.5 ?mol/kg DM and 21.35 ?mol/kg respectively every other day.Two hours later,the animals in the control group were intraperitoneally given the injection of saline.From the second to fourth groups were injected with 12.0 ?mol/kg MMC.The administration of MMC above was given five times a week(from Monday to Friday),for 4 weeks as well as the administration of DM and riluzole every other day before injected with MMC(on Monday,Wednesday and Friday).Twenty-four hours after the last injection,7 rats in each group were sacrificed.The contents of Hg,Ca2+-ATPase in the cerebellum were determined.Results In MMC group,the contents of Hg,Glu and the content of Gln were decreased,the activities of SDH,Na+-K+-ATPase and Ca2+-ATPase were decreased compared with the control group.Compared with MMC group,in DM and riluzole groups,the contents of Hg,Glu decreased,the content of Gln increased,moreover,the activities of SDH,Na+-K+-ATPase and glutamate(Glu),glutamine(Gln) in the rat cortex and the activities of SDH,Na+-K+-AT Pase and Ca2+-AT Pase increased significantly.Conclusion "Glu-Gln Cycle" can be destroyed by MMC,DM and liluzole present some antagonistic effects on MMC-indused neurotoxicity in rats.

Aged ; Aged, 80 and over ; Blood Platelets ; cytology ; Coronary Disease ; blood ; Female ; Humans ; Hypertension ; blood ; Male ; Platelet Count

Objective: To evaluate the role of folic acid supplementation in prevention of colorectal adenoma recurrence. Methods: Weconducted computer search of the Cochrane Central Register of Clinical Trails, Pubmed, Embase, CBMdisc and CNKI. Hand search of relevant journals and conference proceedings was also conducted. Based on the predefined inclusion and exclusion criteria, randomized controlled trials(RCT) about folic acid in treatment of patients after adenoma resection were included in the present study. After evaluating the quality of studies, meta-analyses were performed by RevMan 5. 0. 24 software for the pooled recurrence risks of colorectal adenoma and progressive colorectal adenoma. Results: A total of 6 RCT studies, including 2,002 patients, were enrolled in this study. The results of meta-analysis showed that the recurrence risks of colorectal adenomas were not significantly different between folic acid supplementation and placebo groups [RR=0.97, 95%CI(0.79, 1.19), P = 0.76]; but there were significant differences in the risks of progressive adenomas between the two groups[RR=1.33, 95%CI (1.01, 1.74), P = 0.04]; and this effect of folic acid supplementation appeared when 1 mg/d folic acid supplementation was applied for ≥3 years [RR=1.49, 95%CI(1.07, 2.09), P = 0.02]. Conclusion: Folic acid supplementation fails to reduce the risk of colorectal adenoma recurrence, and it might increase the recurrence risk of advanced adenoma.

10-15 years were 7 cases(6.3%),they were randomly divided into treatment group and contol group.The treatment group with 60 patients were given INF-? and polyresistin,age 3 years,given IFN-? 200 000 U per day;and polyresistin 5 mg per time,3 times every day,the course of therapy were 5 days.The control group was given traditional Chinese medicine at the same time.Results After therapy,in treatment group,53 cases(88.3%) of children′s cooling time were lower than 5 days,47 cases(78.3%) of children′s rash subsided time were lower than 6 days,48 cases(75%) of children′s Catarrhal symptoms disappeared time were lower than 5 days,and the rate of complications′ occurrence were 15 cases(18.3%).In control group,28 cases(53.8%) of children′s cooling time were lower than 5 days,14 cases(26.9%) of children′s rash subsided time were lower than 6 days,28 cases(53.8%) of children′s Catarrhal symptoms disappeared time were lower than 5 days,and the rate of complications′ occurrence were 37 cases(71.5%),there were significant differences between both groups(Pa

OBJECTIVETo systematically evaluate the effectiveness of integrative medicine (IM) for preventing postoperative recurrence of primary hepatic carcinomas (PHC).

METHODSSuch databases as The Cochrane Library, PubMed, EMbase, China National Knowledge Infrastructure (CNKI), VIP Database, Wan-fang Database were retrieved to collect randomized controlled trials (RCTs) on preventing postoperative recurrence of PHC by IM. The retrieval period started from database establishment to June 2013. Literature screening, data extraction, and quality evaluation were performed by two reviewers strictly according to inclusion criteria. Meta-analysis was conducted using RevMan 5.2 Software.

RESULTSA total of 5 RCTs involving 367 patient cases were included. Results of Meta-analysis showed that the IM group was superior to the Western medicine (WM) group in the 1-, 2-, and 3-year recurrence rates (P < 0.05).

CONCLUSIONIn the prevention of postoperative recurrence of PHC, the effectiveness of IM was more significant, when compared with treatment of Western medicine.

Drugs, Chinese Herbal ; therapeutic use ; Humans ; Integrative Medicine ; Liver Neoplasms ; prevention & control ; Neoplasm Recurrence, Local ; prevention & control ; Postoperative Period ; Randomized Controlled Trials as Topic ; Treatment Outcome

Objective: An automatic seamless stitching method with spinal X-ray image sequence is presented in this paper. Methods: First, biorthogonal wavelet transform is used to implement decomposing of the multi-resolution and the effective edge of the image can be extracted by this method combined with Canny operator. The feature points of the image can be obtained by calculating the edge contour matrix E and the value matrix H. Second, the roughly matching of feature points can be achieved by using Normalized Cross Correlation (NCC) algorithm and the random sample consensus (RANSAC) algorithm is introduced to remove false matching pairs and to achieve precisely matching. Third, the image sequence is automatically sorted with the improved genetic algorithm to achieve automatic stitching. At last, the weighted average fusion algorithm is appfied to achieve smooth and seamless image stitching. This algorithm is robust for the weak-contrast X-ray image sequence. Results: Experimental results show that high-quality and fast image sequence stitching can be obtained automatically by using this method. Conclusions: To a certain extent, it overcomes the shortcomings of X-ray image sequence such as the strong image noise, concentration of values ofpixels, blurred boundaries, large overlap area and the sequence constraint, and therefore it may be applied to in medical imaging field widely.