This article described the current status of problems in quality and safety in TCM industry. It also emphatically discussed the quality and safety control in TCM production process. In addition, this article used the HACCP (Hazard Analysis and Critical Control Points) system to conduct a hazard analysis on TCM production process and determined the critical control points, and carried out the corresponding control measures, with a purpose to realize effective regulation and control of hazard factors and ensure TCM quality and safety.

[Objective] To introduce the Chiari osteotomy combined with bone grafting shelf procedure and scew fixation for the treatment of acetabular dysplasia which was caused by all sorts of reasons.[Methods]Totally 56 patients(63 hips)with acetabular dysplasia were operated by Chiari osteotomy combined with bone grafting procedure and screw fixation from October 1982 to October 2001,the average age of the patients was 20.7 years(8~42 years).There were 7 males who didn 't have acetabular dysplasia of both hips,49 females in which 7 ones had acetabular dysplasia of both hips.The X-ray graphies before operation showed:average CE angle was 4?(-20?~ 18?),femoral head coveragement ratio was 60%(42%~75%).Sharp angle was 51?(40? ~58?),AC angle was 27?(20?~38?).All of the patients had subluxation of hip(broken Shentions line)of different degrees except two.[Results]Thirty-two patients(37 hips)had followed up results for average 45 months(6 months to 8 years).Thirty hips were obviously pain-free.The average CE angle was 44?(41? ~62?),Shape angle was 37?(30?~45?)AC angle was 12?(8? ~18?),Harris hip score was increased from 76.3(61~82)before operation to 89(76~95)after operation.Two hips had the complication of bone absorbtion after operation.[Conclusion]The Chiari osteotomy combined with bone grafting shelf procedure and stew fixation is a better procedure for the treatment of acetabular dysplasia,the main advantages of it are as following:(1)The injury during the operation is light without much blood lost,blood tranfusion is not needed.(2)It has the merits of Chiari osteotomy.(3)It can increase the femoral head coveragemean efficiently.The diameter of acetabular from anterior to posterior and the diameter form left to right can be increased.(4)It is not easy for the bone grafted to the absorbed.(5)It works in the case which can 't be absolved by only Chiari osteotomy.

Objective To study the protective effect and mechanism of safflower solution on warm ischemia reperfusion injury(IRI) of liver in rats.Methods Male Sprague-Dawley rats were randomly divided as 4 groups: Group S(sham group);group I/R(IRI group);group IPC(ischemic preconditioning group);group SPC(safflower solution preconditioning group).The rats were sacrificed at 24 h after reperfusion,serum levels of ALT and AST were measured and HE staining of liver tissues were made to detect rat liver histological changes and grade liver IRI(Suzuki score);apoptosis were monitored by TUNEL;TNF-?,MIP-2 and ICAM-1 mRNA of liver were measured by RT-PCR;NF-?B gene products of liver were detected by Western blotting.The mean value of all the assay tests was compared.Results After 24 h reperfusion,compared with group I/R,the liver function(ALT and AST) in group SPC and group IPC was significantly improved(P

Game relationship of mutual restriction and mutual connection exist among various segments of Chinese medicine decoction pieces supply chain. Any problems in any segments will affect quality and safety of the whole supply chain, and ultimately affect the safety of consumers. Based on the theory of supply chain, this article established a linear supply chain and static game model, including planting farmers, production and marketing society, processors of Chinese medicine decoction pieces, terminal consumers and middle sales section whichis made up of whole sale and retail markets and manufacturing enterprises of Chinese patent medicine. This article also proposed the suggestions about quality safety of Chinese medicine decoction pieces through quantitative methods in game theory.

Objective: To compare the curative efficacy and safety of electro-acupuncture on Jianyu (LI 15) with oral administration of Diclofenac Sodium Sustained-release Capsules in treating shoulder periarthritis. Method: Randomized controlled trials (RCT) were adopted in the study. The patients were randomized into two groups, 30 cases in one group.Electroacupuncture was done on Jianyu (LI 15) 20 min every time in the treatment group,while 75 mg Diclofenac Sodium Sustained-release Capsules were orally administered in the control group. For all the patients in two groups, one treatment course contains 7 days. Then the curative efficacy was evaluated by the efficacy evaluation criteria after two consecutive courses. Result: The total effective rate of treatment group and control group were 93.3% and 56.7% respectively. Conclusion: Electroacupuncture on Jianyu (LI 15) is an effective therapy for shoulder periarthritis and has more significant effect than oral Diclofenac Sodium Sustained-release Capsules.

Objective To explore the expressions of LAT and VEGF, and the relationship between them in hepatocellular carcinoma (HCC). Methods The expressions of LAT protein and VEGF protein in SO HCC, 30cirrhosis and 10 normal tissues were assessed by immunohistochemical method. Results The expressions of LAT and VEGF in cancer tissues, cirrhosis tissues, normal tissues were significantly different. The expression of LAT in HCC tissues was stronger than that in cirrhosis, but the expression of VEGF in cirrhosis was stronger than that in HCC tissues. Conclusion The expression of LAT was not closely associated with the expression of VEGF in HCC. The abnormal expressions of LAT and VEGF were closely associated with the development of HCC. They may play important roles in development of HCC.

Objective To clarify the role of claudin-4 in endometrial tumorigenesis and explore claudin-4 be as potentially useful agent in the treatment of endometrial carcinoma.Methods The expression of claudin-4 in 62 endonetrioid endometrial carcinoma (EEC),30 atypical hyperplasia endometrial tissue and 60 human normal endometrium was determined using immunohistochemistry and realtime PCR.Ninety female BALB/c mice were transplanted with Ishikawa endometrial cancer cells,which were divided into three groups with different intraperitoneal treatments with cisplatin,paclitaxel and saline solution.After the observation period,the tumors were extracted and stained with monoclonal antibody against claudin-4.The messenger RNA expression of claudin-4 was also detected using real-time PCR.Results Among the EEC samples,34％ (21/62) showed medium staining for claudin-4 and 66％ (41/62) showed intense staining.In atypical hyperplasia group,27％ (8/30) showed weak staining,53％ (16/30) showed medium staining and 20％ (6/30) showed intense staining for claudin-4.Of the normal endometrial tissue,47％ (28/60) showed weak staining and 53％ (32/60) showed no staining for claudin-4.According to real-time PCR,the relative quantity of claudin-4 was 170 ± 12 in EEC group,89 ± 15 in atypical hyperplasia group and 18 ± 3 in normal endometrium.Compared with those in atypical hyperplasia group and normal endometrium group,the protein and mRNA expression of claudin-4 were significantly increased in the group of EEC (all P ＜ 0.05).In the study of Ishikawa xenografts,no significant changes in tumor volume and claudin-4 expression were shown in paclitaxel group compared with that in the control group.Nevertheless,a significant reduction of the tumor growth and a significant decrease in claudin-4 expression were observed in cisplatin group.After cisplatin treatment,the tumor volume was significantly decreased [(0.51 ±0.21) versus (0.73 ±0.12) cm3],and the mRNA expression of claudin-4 was also significantly decreased (153 ± 35 versus 273 ± 27).Conclusion These results demonstrate that claudin-4 is strongly expressed in EEC,which may be a useful biomarker to monitor the effects of chemotherapy in patients with endometrial carcinoma.

The inclusion compound of amylose and salicylic acid (SA) was prepared by a sealed temperature control method, and the formation of the inclusion compound was confirmed by IR spectrum and powder X-ray diffraction. The kinetic parameters of dissociation of amylose/SA compound were studied by the nonisothermal method which was defined as a relationship between the dissociation ratio and time. The values of activation energy (Ea) and frequency factors (InA) were calculated by a nonlinear least-square method. In this study, the formation of the inclusion compound of amylose/SA was confirmed by IR spectrum powder X-ray diffraction. SA existed in a molecule form in the spiral stouction of amylose. The dissociation of amylose/SA compound was attributed to first order reaction. The values of Ea calculated by the nor-isothermal method were 21.71 and 22.41 kJ x mol(-1) at heating rate 5 and 10 degrees C x h(-1), respectively. The corresponding isothermal method value of Ea was 22.17 kJ x mol(-1); the calculated InA values were 9.32 and 10.08 at heating rate 5 and 10 degrees C x h(-1), respectively. The corresponding isothermal method lnA value was 9.26. The results were in good agreement with Ea values and lnA values by isothermal method. These results indicated that the non-isothermal method described in this study could be adequately used for the stability study of inclusion compound and was a rapid and accurate method for the determination of kinetic parameters.

AIM:To evaluate the morphological and functional changes of retinas induced by treatment of tunicamycin with optical coherence tomography (OCT) in rats.METHODS:Totally 60 SD rats were randomly divided into 3 groups (20 in each group), 0.5mg/kg (in low dose group), 1.5mg/kg (in high dose group) tunicamycin were injected into vitreous cavity and saline (9g/L NaCl) were injected in the same dose as a control group.Changes of retinas were observed by OCT on the 1,7 and 14d after treatment of tunicamycin.Then the rats were sacrificed, retinas were taken out and embedded by the paraffin, tissue sections and the HE staining were performed.RESULTS:OCT results suggested that tunicamycin played damage effects on retinal morphology and structure which appeared a time-and dose-dependent.Fundus photography results suggested that 2wk after tunicamycin treatments, with the gradually changing of tunicamycin concentration, peripheral retinal and macular region became pale color gradually, edema occurred in optic disk, retinal vessels appeared thinner in the high dose group, optic nerve came out atrophy.Fluorescein angiography confirmed that tunicamycin injection in vitreous cavity 2wk later, retinal vessels injury occurred, resulted in leaking of intravascular contrast agent from peripheral to the central part of the retinas.Electrophysiological data showed that retinal electrogram occurred disorder induced by tunicamycin, such as the amplitude of a wave, b wave decreased gradually, even closed to zero, which was very different from control significantly (P<0.05).HE staining of paraffin sections showed that retina injuries induced by tunicamycin were in dose-time dependent, which was consistent with the results of OCT.CONCLUSION: Clinical retinal diseases could be simulated by retinal damage animal model induced by tunicamycin treatment.OCT detection offered real-time images of the retinal cross-section, which provided a helpful non-invasive method for detecting and evaluating the retinal damages.