Biomarkers ; Hepatitis B ; therapy ; virology ; Hepatitis B Surface Antigens ; Hepatitis B virus ; Humans

Child ; Cranial Nerve Injuries ; drug therapy ; etiology ; Diagnosis, Differential ; Female ; Glossopharyngeal Nerve ; pathology ; Humans ; Malaria, Cerebral ; complications ; diagnosis ; drug therapy ; Vagus Nerve ; pathology

Membrane fusion is essential for all life activities of eukaryotes and the fusion process requires the interaction of different vesicular transporters,which are highly conserved in eukaryotes,to coordinate specifically and facilitate the fusion of different biofilms. Vesicle associated membrane protein 8(VAMP8)mainly locates in vesicular as well as lysosomal membranes,and plays a vital role in the fusion of many different biofilms. This paper focused on the molecular structure,transcriptional regulation,post-translational modification and biological function of VAMP8 as well as the research progress on its correlation with human diseases,so as to provide new ideas for the treatment of related diseases and the development of effective therapeutics targeting VAMP8.

ObjectiveTo investigate the relationship among social support,coping style and mental health on university librarians.Methods421 librarians were assessed with the symptom checklist 90 (SCL-90),social support rating scale (SSRS) and simplified coping style questionnaire (SCSQ) in 2005. ResultsSome factor of SCL-90 negatively correlated with utility percentage of social support(P<0.05), and positively correlated with passive coping style(P<0.05). Except utility percentage,the other three factors of social support showed significant difference among different academic titles (P<0.05), and it was significant different (P<0.05) on passive coping factor among them. The score of social support improved with ages except for utility percentage (P<0.05).On positive coping factor but not negative, there were significant difference among different age groups(P<0.05). ConclusionThe social support and coping style obviously correlate with the mental health of university librarians.

Objective To investigate the clinical effect of sitagliptin combined with metformin in the treatment of sulfonylurea-deficient elderly patients with diabetes mellitus.Methods Sixty patients with diabetes mellitus were enrolled in our hospital from January 2013 to December 2016.The patients were divided into observation group(30 cases) and control group(30 cases) according to the random number method.The patients in the observation group were treated with sitagliptin and metformin.The patients in the control group were treated with gemcitabine, and the clinical treatment effect, fasting blood glucose, postprandial blood glucose, glycosylated hemoglobin, fasting C peptide and postprandial 2hC peptide Level of statistical comparison.Results The clinical effect of the observation group was significantly higher than that of the control group.Before and after treatment, the fasting blood glucose, 2h postprandial blood glucose, glycosylated hemoglobin, fasting C peptide and postprandial 2hC peptide level were not significantly different After fasting blood glucose, 2h postprandial blood glucose and glycosylated hemoglobin levels were significantly lower than before treatment, after treatment of fasting C peptide and postprandial 2hC peptide levels were significantly higher than before treatment.Conclusion The clinical efficacy of serotretine combined with metformin in the treatment of elderly patients with diabetes mellitus is similar to that of Yuxing, and the combination is more convenient and more suitable for clinical treatment.It is worthy to be popularized and applied in clinical practice.

Objective: Hitherto there has been no report on the changes of microvessels and the expression of angiogenic factors after photodynamic therapy(PDT) of liver carcinoma.The purpose of this study was to investigate the effect of PDT on the microvessels and the expression of vascular endothelial growth factors(VEGF) in tumor cells. Methods: The mice models subcutaneously transplanted with Heps liver carcinoma were randomly divided into a PDT group(n= 28) and a control group(n= 28).The dynamic changes of the microvessels and VEGF expression in the tumor cells were observed at 2,6,24 and 72 h after PDT by H-E and immunohistochemical staining.Results: Progressive changes were seen in the tumor microvessels at the four time points after PDT,including dilatation,blood stasis,hypostasis,hemorrhage,and angiolysis.Compared with the control group,the microvessel density of the tumors was reduced significantly(P

The incidence of swallowing disorder is high in previous research both in oral and pharyngeal stage, and there are many related factors contributing to the assessment of swallowing function. This article reviewed the research progress of dysphagia after traumatic brain injury (TBI) from epidemiology, assessment methods, characteristics, risk factors, therapeutic strategies and clinical outcome。

Objective:To analyze the clinical drug consultation to summarize the characteristics of drug consulting services and improve clinical pharmacy service quality.Method:756 complete cases of clinical drug consulting materials were analyzed and summarized in our hospital emergency in the past three years.Result:The major consulters were patients, accounting for 59.92 percent.The most often consulted anti-infective drugs accounted for 35.85 percent.The primary attention was given to usage and dosage of drugs,accounting for 12.96 percent.Conclusion:The drug consultation lets us fully understand the issues most concerned about by medical personnel and patients,makes pharmacists improve their professional level and makes sure that patients are given reasonable,safe and effective medication.

Objective To establish two PCR assays to rapidly and simultaneously detect U.parvum and U.urealyticum.Methods Two PCR assays were established through designing two sets of specific primer targeting urease gene B of U.parvum and U.urealyticum,respectively.The standard strains of U.parvum and U.urealyticum and the clinical samples were detected by these PCR assays and PCR products of the standard strains and two clinical specimens were directly sequenced and carried out specific and sensitive assays.Forty-eight clinical specimens were tested for culture and the PCR assays and tow methods were compared.Results The standard strains of U.parvum,U.urealyticum and the clinical specimens were successfully and differentially detected by the PCR assays and the sequencing results were found to have a complete similarity to the GenBank sequences.The 14 strains of other bacterial genera including 5 strains that produced urease were not amplified by these PCR assays.Each assay had a detection limit of 10 copies/?l of plasmid DNA.The positive rate in 48 clinical specimens by PCR(54.2%)was higher than that by culture(39.6%)(P

Objective To study the mechanism of protein extracted from nidus vespae protein(NVP) on anti-leukemia and provide experimental and theoretical evidence for exploring new anti-leukemia drugs. Methods The effect of the NVP on cell growth and morphological changes of K562 cells was observed by using cell culture and transmission electronic microscopy,the effect of NVP on apoptosis-associated signal transduct protein NF-? Bp65,?-catenin and iNOS of K562 cells was detected via immunohistochemical method and image analysis system. Results(1) The saturation destinity of K562 cells in NVP group was decreased which suggested that the proliferation was inhibited.(2) In the NVP group typical apoptosis morphological changes were observed in the K562 cells under transmission electronic microscope.(3) The result of immunohistochemistry showed that the expression of NF-? Bp65,?-catenin and iNOS of K562 cells in the NVP group decreased.The optical density(A) values of NF-?Bp65,?-catenin analysed by the image analysis system were decreased in the NVP group.Conclusion The NVP can inhibit the proliferation of K562 cell prominently,and the mechanism probably is that the apoptosis is induced via regulating the expression of apoptosis-associated signal transducted factors NF-?Bp65,?-catenin and iNOS.