Objective To study the distribution and antimicrobial susceptibilities of anaerobic bacteria from periodontal pockets of patients with chronic periodontitis, so as to guide the anti-infection therapy. Methods Eighty-four patients with chronic periodontitis were enrolled in this study. The anaerobes were isolated from periodontal pockets of patients with chronic periodontitis. Antimicrobial susceptibility of the anaerobes to seven kinds of antibiotics (like metronidazole, penicillin, cefmetazole, etc.) was tested with the agar dilution technique. Results A total of 305 cultivable anaerobes were isolated from periodontal pockets of 84 patients with chronic periodontitis. All patients were infected by anaerobes. 59.34%of cultivable anaerobes were gram-positive anaerobes, and 40.66%were gram-negative anaerobes. The P.melanin ogenica and P.gingivalis were the most common among the cultivable anaerobes, accounting for 51.19% and 55.95%, followed by P.intermedia (36.90%), P.micros (35.71%), F.mucleatum (33.33%), Capnocytophaga ssp (27.38%), Actinomyces ssp (10.71%), and so on. Most isolated anaerobes were sensitive to seven antibiotics. All isolated anaerobes were susceptible to Chloromycetin and Imipenem. 98.03%of isolated anaerobes were susceptible to Amoxicillin. Approximately 76%to 88%of isolated anaerobes were susceptible to Cefmetazole and Metronidazole. Susceptibility to Clindamycinum and Penicillin were 65.91%and 55.73%. Conclusions There is a close relationship between chronic periodontitis and anaerobes. P.melanin ogenica and P.gingivalis are the most frequently isolated anaerobes from periodontal pockets of patients with chronic periodontitis. Chloromycetin and Imipenem can be used for the treatment of anaerobes infected chronic periodontitis.

Objective: To observe the histomorphology/pathology changes and the expression of hypoxia inducible factor-1 alpha (HIF-1?) and vascular endothelial growth factor (VEGF) in drowning rats brain, in an attempt to explore new pathways for treatment of water drowning. Methods: The animal model of freshwater drowning was established with rats. The rats were randomly divided into the following groups: control group, drowning-to-death group and 2 min-drowning group (including 5 subgroups survived for 3, 6, 12, 24. and 40 hours after being drowned in water for 2 min [n=10]). Brain indices of rats in each group were detected and the pathological changes of cerebral tissues were observed; the expression of HIF-lo and VEGF were detected immunohistochemically and the correlation between them was analyzed. The dynamic blood oxygen saturation was detected in rats survived for 40 h after 2 min-drowning at different points. Results: Brain index of the drowning group and groups surviving for 24, 40 hours after drowning were obviously higher than that of the control group (P

Objective To evaluate the effect of controlled hypotension with nitroglycerin and esmolol in the operation of endoscopic nasal surgery.Methods 60 patients undergoing selective endoscopic nasal surgery were randomly divided into three groups averagely: control group (groupⅠ), controlled hypotension with nitroglycerin group(group Ⅱ), controlled hypotension with nitroglycerin and esmolol group (groupⅢ). Every group has 20 patients.Mean artery pressure (MAP), HR, scores of surgical field quality(SSFQ) were recorded before anaesthesia and 10,20,30,40 minutes after anaesthesia. After the operation, bleeding capacity, operation time and dosage of nitroglycerin were recorded too.Results The bleeding capacity and operation time in groupⅡ and Ⅲ was significantly less than that in groupⅠ(P

Objective To study the TPS dose distribution of the radiation therapy with wedge for upper-thoracic esophageal cancer.Methods Adopting spiral CT scanning,three dimensional data of the focus of infection and the other tissues were acquired.The radiotherapy plans with 45? wedges and without wedge were designed in TPS.The dose-volume histogram were calculated and compared.Results Although the average of dose of irradiated target was very close in the two plans,but there was remarkable difference in the dose uniformity.The Std(standard deviation) of dose in irradiated target was 6.31 in plan without wedge,and it was 4.45 in plan with the 45? wedge.The results show that the dose distri-butions of irradiated target are optimized by using the wedge.Conclusion It is a simple and effective method to use wedge for improving dose distribution in the radiation therapy for upper-thoracic esophageal cancer.

Objective To observe the effect of Ganshu Granule on different models of fatty liver in rats. Methods Rats with acute fatty liver induced by alcohol and carbon tetrachloride, rats with chronic fatty liver induced by alcohol and high-fat diet, and rats with acute fatty liver induced by ethionine were used, and the effects of different doses of Ganshu Granule on serum lipid metabolism, intrahepatic lipid metabolism and histopathological changes in liver were observed. Results In fatty liver rats induced by alcohol and carbon tetrachloride, Ganshu Granule could reduce the level of total cholesterol (TC), triglycerides (TG), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in serum and could decrease total fat and glycogen(Gn) in liver obviously. In fatty liver rats induced by alcohol and high-fat diet, Ganshu Granule could significantly decrease the level of TC, TG, ALP, LDL and ALT in serum and could reduce the content of total fat, TG and Gn in liver obviously. In fatty liver rats induced by ethionine, Ganshu Granule could decrease the level of TC, TG and ALT in serum, raise the level of HDL in serum and could decrease the content of total fat, TG and Gn in liver obviously. The histopathologic results showed the degree of hepatic steatosis and hepatocellular necrosis were lessened in all of the treated groups. Conclusion Ganshu Granule is effective in preventing and treating fatty liver.

The antiarrhythmic actions of ZnSO4 (Zinc) were studied in ex-perimental arrhythmias models. ZnSO4 10mg/kg(iv) significantly coun-teracted the arrhythmias, induced by aconitine or BaCl2 rats.It was shown to shorten the duration of arrhythmias induced by chloroform-epinephrine in rabbits. In mice, ZnSO4 20 ?g/10g(ip) decreased the incidence of ventricular fibrillationbychloroform. However, ZnSO4 did not decrease the incidence of CaCl2-induced , venticular fibrillation, the threshold and lethal doseofouabain in producing ventricular permature beats, ventricular fibrillation in guinea pigs.

Zinc sulfate 0.1mmol/L inhibited the frequency dependent positive staircase phenomenon on isolated guinea pig atria. Ten min after adding zinc sulfate 0.1 mmol/L, the contractility of the left atrium decreased. The needed concentration of epinephrine inducing automaticity was increased and the functional refractory period(FRP) was prolonged from 192?5 ms to 247?4 ms ( P

Objective To study the rule of breaking Ferula fukanensis seed dormancy under low-temperature and content changes of endo-hormone.Methods The seeds were treated with stratification under 4 ℃ and germinated under 20 ℃.The content of endo-hormone,such as Z,GA_3,IAA,and ABA,was mensurated by HPLC.Results The seed germination rate achieved as high as 14% in 20 d and more than 60% in 40 d.Among breaking the seed dormancy,the content of endo-hormone was decreased gradually,while the rate of GA_3 and ABA was increased quickly in 10—20 d under 4 ℃ stratification.ConclusionThe stratification under 4 ℃ could break the seed dormancy.The rate of GA_3 and ABA is a pivotal factor of the seed dormancy.The endo-hormones IAA and Z have the significant effect on seed germination.

Behcet Syndrome ; Child ; Humans ; Intestinal Diseases ; Male