Background Fungal keratitis can cause serious damage to visual function of corneal infective disease,which is more difficult to treat.In recent years,injecting antifungal drugs to the corneal stroma not only enrich the treatment of the disease,but also achieve good clinical effects.Correctly selecting drug kinds and drug concentration can improve the cure rate,and reduce adverse reactions after treatment,but the related research is rare.Objective This study was to observe the clinical effects of corneal stroma fluconazole injection with different concentration for the treatment of fungal keratitis.Methods Prospective study was performed.One hundred and two patients (102 eyes) diagnosed as fungal keratitis were included from May 2012 to January 2015 in Jizhong Energy Xingtai Mining General Hospital.The patients were randomly divided into 3 groups,The eyedrop treatment group (29 eyes) received 0.5％ fluconazole eyedrops and 5％ natamycin eyedrops treatment.The 0.1％ fluconazole group (35 eyes) and 0.2％ fluconazole group (38 eyes) received 0.1％ and 0.2％ fluconazole corneal stroma injection after eyedrop treatment,respectively.Each group underwent potassium hydroxide wet examination and fungal cultures.The curative effect and adverse reactions were observed.Results Fusarium 41.2％ (42/102),Aspergillus 21.6％ (22/102)and Alternaria mold 17.6％ (18/102) ranked the top three pathogenic species.The distribution of pathogenic fungus among the 3 groups were significantly different (x2 =3.763,P＞0.05).The cure rate of eyedrop treatment group was 44.8％ (13/49),which was significantly lower than 0.1％ fluconazole group (74.3％,26/35) and 0.2％ fluconazole group (81.6％,31/38) (x2 =5.782,9.854;both at P＜0.05).The cure rate was significantly different between 0.1％ fluconazole group and 0.2％ fluconazole group (x2=0.566,P＞0.05).The average cure time of eyedrop treatment group,0.1％ fluconazole group and 0.2％ fluconazole group were (36.28 ± 10.39),(29.14± 7.86) and (21.34 ± 8.57) days,respectively,with a significant difference among the three groups (F =5.336,P=0.006).The acuity of vision was significantly increased after treatment in the 0.1％ fluconazole group and 0.2％ fluconazole group (t =3.009,4.695;both at P ＜ 0.01).The average number of injection in the 0.1％ fluconazole group was (5.71 ± 2.97) times,which was higher than (5.13 ± 1.80) times in the 0.2％ fluconazole group (t=4.471,P＜0.05).Four cases in 0.2％ fluconazole group with eye irritation were observed.After diclofenac sodium eyedrops treatment,the symptoms of 3 cases were disappeared.One case was cured by conjunctival flap covering method.All the cases were followed up for 1-3 months,no adverse reactions and recurrence was found.Conclusions Corneal stroma fluconazole injection is an effective method for treatment of fungal keratitis.In order to reduce the occurrence of adverse reactions,0.1％ fluconazole injection in corneal stroma is recommend for light fungal keratitis patients;for moderate and heavy fungal keratitis patients,0.2％ fluconazole injection in corneal stroma can be performed after failure of 0.1％ fluconazole treatment.

Objective To observe the protective effect of Mongolian medicine Carthamus tinctorius in acute liver injury induced by D-GlaN(D-galactosamine)in rat models. Methods The acute liver injury model was established by intragastric administration D-GlaN in rats. The levels of serum aspartate aminotransferase(AST),alanine aminotransferase(ALT),glutathione(GSH-Px),the content of tumor necrosis factor-α(TNF-α)in liver tissue and the apoptosis of hepatocytes were determined. Results Mongolian medicine safflower can significantly reduce the activity of serum ALT and AST and liver tissue GSH-Px,and the degree of cell apoptosis;however,no obvious change of the TNF-αcontent was observed. Conclusion The Mongolian medicine Carthamus tinctorius has protective effect in D-GlaN-induced acute liver injury rats probably via anti-oxi-dative and anti-apoptotic abilities.

Objective To study the role of corn oil in the change of serum glucose, serum lipids and insulin in type 2 diabetes rats with insulin resistance, and attempt to find out its mechanism. Method High fat and sugar diet plus low-dose streptozotocin (STZ) was given to establish type 2 diabetes rat with insulin resistance model, the rats of which were randomly divided into normal group, model group, corn oil (0.8 mg/kg) group, rosiglitazone group. After Ig 6 weeks, fasting blood glucose (FBG), total cholesterol(TC), triglyceride(TG), low density lipoprotein(LDL), high density lipoprotein (HDL), fasting insulin (FINS), and insulin resistance index (HOMA-IR) were detected. Results Corn oil can elevate the level of HDL in diabetic rat, lower the levels of FBG, TC, TG, LDL, FINS and HOMA-IR. Conclusion Corn oil can improve insulin resistance, and decrease the serum glucose and lipid of type 2 diabetic rats.

Enlightened by the essay Affection of Nutrition Factors On Endemic Fluorosis written by professor Li Guang-sheng ,and integrated with the contradictory phenomena in three times epidemic invest igations,the author thought:in epidemic factors of fluorosis,endemic fluorosis not only is related with fluoride intake perday,but also related with nutritional factors,especially calcium in food.The article discussed on countermeasures of prvention of endemic fluorosis in views of the above.

Background and purpose:The epidermal growth factor receptor (EGFR) is commonly overexpressed in a variety of solid tumors, and has important roles in cancer pathogenesis and progression. EGFR thus provides a rational target for cancer therapy. We studied siRNA-mediated inhibition of epidermal growth factor receptor expression and its biologic effects in different human cancer cell lines (A431, HeLa and SPC-A-1). Methods:Cells were transfected with chemically synthesized siRNA-EGFR. EGFR mRNA was quantified by real-time PCR and was detected by immunofluorescence staining and flow cytometry. The biologic effects on cell growth were assessed by colony-formation assay.Results:siRNA-EGFR significantly decreased mRNA level of EGFR by 73.9 %, 44.6 % and 57.7 %, protein expression of EGFR by 77.0 %, 61.3 % and 65.2 %, and reduced colony number by 27.2 %, 53.9 % and 59.1 % in A431, HeLa and SPC-A-1, respectively.Conclusions:Our data suggested that RNA interference could downregulate EGFR and inhibit colony forming ability and EGFR expression at mRNA/protein levels in human cancer cell lines with different pathological types. siRNA could be one of the promising strategies in future targeted cancer therapy.

Objective To evaluate the value of spatio-temporal, image correlation (STIC) in prenatal diagnosis of complex heart malformations.Methods STIC was used to examine 25 fetuses with 20-30 weeks of gestation who were doubted with complex heart malformations prospectively by two-dimensional (2D) echocardiography.Dynamic three-dimensional imaging was achieved by postprocessing technique with STIC across the entire fetal heart and thorax.An offline analysis of the acquired volume was carried out to examine the fetal cardiac anatomy with multiplanar mode, surface rendering and minimum mode.The results of prenatal diagnosiswere compared with postnatal color Doppler and surgical findings.Results Successful STIC volume datasets were obtained in all 25 fetuses.The findings of all 25 fetus with complex heart malformations included double outlet of right ventricle in 1 case, truncus arteriosus in 1 case, complete transposition of the great arteries in 3 cases,corrected transposition of the great arteries in 1 case, complete endocardial cushion defect in 4 cases, hypoplastic right ventricle syndrome in 1 case, hypoplastic left heart syndrome in 2 cases, tricuspid atresia in 1 case,pulmonary atresia in 1 case, tetralogy of Fallot in 3 cases, single atrium in 1 case, single atrium and single ventrium in 4 cases,in 1 case, atrial septal defect and ventricular septal defect with coronary sinus dilatation in 1 case.The major diagnoses derived from STIC in 23 of 25 cases were consistent with the results of postnatal color Doppler and surgical findings.Conclusions STIC can provide more accurate information of structures of complex fetal heart malformations and has great value in pregnant prognosis.

Objective To construct expression vectors that Renilla reniformis (Rluc) fused with neurotensin type 1 receptor (NTSR1),and to investigate the interaction between NTSR1 and other receptors,as well as intercellular signal transduction mechanism mediated by neurotensinl-R.Methods The human NTSR1 gene was amplified by PCR using the plasmid pcDNA3.1-hNTSR1 as template.The PCR product was digested,ligased with the plasmid pRluc and then be transformed into the competent cell Top10.The construct was identified by DNA sequencing.The recombinant plasmid was transiently transfected into human embryonic kidney 293 ( HEK293 )cells,and the expression of pRluc-hNTSR1-pcDNA3.1 was detected by confocal microscopy and Western blot.Results The fragment of 1257 bp was amplified by PCR,and the DNA sequences were identical with the gene in GenBank ( NM_002531 ).Western blot showed a band about 90kDa.Confocal microscopy showed that NTSR1 was expressed on the plasma membrane.Conclusion The pRluc-hNTSR1-pcDNA3.1 eukaryotic expression vector is successfully constructed,and the expression vector can be used to investigate the interaction between NTSR1 and other receptors,as well as intercellular signal transduction mechanism mediated by neurotensinl-R,which will provide new target for drug development.

Objective To investigate how the independent components(ICs)energies of multichannel local field potentials(LFPs) code event base on the analysis of ICA of the cortical LFPs of rats. Methods Taking the event point as the zero point, 15-channel LFPs between the span of ±500ms recorded from the prefrontal cortex of rats were decomposed into 15 ICs. The energies of the ICs were computed in a 50-ms window. By sliding the window with step of 25 ms, a dynamic distribution mapping of the 15 ICs' energies was established. ICs with distinctly increased energies during the span of ±200 ms, which indicating that these ICs energies coded event,were selected as the targets. The corresponding channels of these ICs were determined consequently via the inverse transformation of ICA. Results Considering each trail of the repetitious analysis for the same segment of data, the spatial localization of the dominate function region(s) turned out to be relatively stable in spite of the uncertainty of the number and sequence of the target IC(s) due to the ambiguities of the decomposition of ICA.Meanwhile, the analysis results of a series of data segments showed satisfactory correspondence between data segments and dominate function regions. Conclusion The ICs' energies of multichannel LFPs are able to code events in working memories; It is valid for ICA to identify the coding patterns of multichannel LFPs to events; ICA is capable to localize the dominate function regions of event coding with satisfactory robustness.

We have trcated 40 cases of active peptic ulcer with Omeprazolc from Jun. 1990 to Mar. 1991. Compared with it, We have treated 40 cases of active peptic ulcer with Ranitidine during the same time. 80 patients with active peptic ulcer were divided into two groups. The age, scx and size of ulcer between the two groups have been compared. The ulcer diameter was from 3 to 20mm. The number of the ulcer was from 1 to 5.40 patients were treated with Omeprazole 20～40mg p. o. everyday. After a course of two weeks trcatment, the healing rate was 95%; 40 patients were treated with Ranitidine 300mg p. o. everday. After a course of six weeks the healing rate was 63%, The difference of the two groups was marked (P

OBJECTIVETo study the effects of sacral nerve root electrostimulation (SNS) on the colon function and its mechanisms in rats with spinal cord injury (SCI).

METHODSOne hundred and four Wistar rats were divided into three groups: A, B and C. A group ( n = 24) was divided into three subgroups (n = 8) for studying the bioelectricity: Normal group (NG), SCI group (SCI) and SCI group with SNS(SNS); B group( n = 24) was divided into three subgroups( n = 8) for studying the colon motility: NG, SCI and SNS. C group( n = 56) were divided into three groups for studying the change of morphology and neurotransmitters(SP and VIP): NG (n = 8), SCI (n = 24), and SNS (n = 24) . In SCI and SNS, included of three subgroups: 24, 48, 72 h after spinal cord injury (n = 8).

RESULTSIn SCI group, the activity of bioelectricity in proximal and distal colon was reduced; the colon motility was lessened, and colon mucosa appeared different degree of damage; cell-cell connections between intestinal epithelial cells were destroyed. The expressions of substance P(SP) and vasoactive intestinal peptide (VIP) in colon were decreased obviously. SNS was found to activate the bioelectricity, promote the colon motility, improve the intestinal mucosal, and increase the expressions of SP and VIP. Conclusion: SNS can activate the peristalsis, rehabilitate the motility of denervated colon, protection of the intestinal mechanical barrier between intestinal epithelial cells and tight junction, rebuild the colon function through activating the bioelectricity and increase the expressions of SP and VIP.

Animals ; Colon ; physiopathology ; Electric Stimulation Therapy ; Epithelial Cells ; drug effects ; Intestinal Mucosa ; drug effects ; Lumbosacral Region ; innervation ; Neurotransmitter Agents ; metabolism ; Rats ; Rats, Wistar ; Spinal Cord Injuries ; therapy ; Substance P ; metabolism ; Vasoactive Intestinal Peptide ; metabolism