ObjectiveToinvestigate the effect of different needling methods on ischemic stroke, for seeking the effective treatment for stroke.MethodBy following the randomized controlled principle, the sixty enrolled patients were randomized into an electroacupuncture groupand an acupuncture group, 30 cases in each group. The two groups were both intervened by needling treatment in addition to the conventional internal medicine management, electroacupuncture for the electroacupuncture group,and ordinary acupuncture for theacupuncture group. The Neuropathy Disability Scale (NDS), Fugl-Meyer Assessment (FMA), and Barthel Index (BI) were adopted for evaluation and comparison.ResultThe NDS, FMA, and BI scores were significantly changed after treatment in both groups (P<0.01); there were significant differencesin comparing FMA and BI scores between the two groups (P<0.05).ConclusionElectroacupuncture can produce a more significant efficacy in treating ischemic stroke compared with ordinary acupuncture, electroacupunctureis also better than ordinary acupuncture in improving the neural deficit.

Objective:To provide some experimental basis of cell biomechanics for clinic treatment of massage by exploring synthesis and liberation of NO and IL-1? from fascia tissue cells of rat under pressure stimulation in vitro.Methods:Fascia tissue cells of rat were cultured in vitro.After morphological identi cation,50kPa pressure was forced on these cells,then the contents of NO and IL-1? in culture medium were detected.Results:The fascia tissue cells are mainly made up of broblast cells.The contents of NO and IL-1? in culture medium were both increased signi cantly under pressure stress.Conclusion:Fascia tissue cells can receive the mechanical stimulation,and transform the mechanical signals into biological signals.The reaction to mechanical stimulation may be one of the cell biomechanical principles of massage.

BACKGROUND: The biological behaviors of preadipocytes in adipose tissue of young animals have been closely linked to the onset and prevention and treatment of obesity. Observing mechanical oscillation effects on biomechanical behaviors of preadipocytes using biomechanical stimuli would provide more direct experimental evidence for treatment of simple obesity using manipulation and massage therapy.OBJECTIVE: Different frequencies of mechanical force stimulation were performed on preadipocytes from young rats cultured in vitro to observe the changes in cell viability, proliferation, and apoptosis.METHODS: Preadipocytes from SD young rats were in vitro cultured. Following identification, preadipocytes were dynamically, mechanically stimulated through the use of constant temperature oscillator. According to different treatment frequencies, three groups were set: 0 Hz (blank control), 1.5 Hz, and 3 Hz. A 30-minute oscillation, once every 12 hours, total 3 days, was performed in each group. Following dynamic mechanical stimulation, cell viability, proliferation, and apoptosis in young rats were observed. RESULTS AND CONCLUSION: In the initial stage of culture,cells exhibited the morphology similar to fibroblasts. After oil red O staining, red particles appeared in the cells, indicating that the young mouse cells cultured in vitro were preadipocytes. With stimulation of oscillation force, the viability and proliferation of preadipocytes were significantly inhibited (P < 0.05 or P < 0.01). There wasno significant difference in effects of mechanical oscillation on preadipocyte apoptosis between 0 Hz and 1.5 Hz, 3 Hz groups (P > 0.05). These findings indicate that thecell biological mechanism underlying preventing simple obesity in adolescents is to inhibit the viability and proliferation of preadipocytes

Objective:To determine the expression level of bone morphogenetic proteins-4 (BMP-4) and the relationship between the expression of BMP-4 and the clinical/pathological parameters in patients with gastric cancer. Methods:Using im-munohistochemical staining technique ,expressions of BMP-4 were investigated in different tissues from 90 gastric cancer specimens and 30 specimens from normal gastric mucosa as control. ResultS:The positive rate of BMP-4 was 23. 3% (21/90) in gastric cancer and 3. 3% (1/30) in normal gastric mucosa. The expression of BMP-4 in gastric cancer was closely related with locus, serosa infiltration,cell differentiation and lymph node metastasis (P

Objective To construct an eukaryotic expression vector of human telomerase reverso transcriptase (hTERT) gene specific shRNA, and investigate the effect of pshRNA-hTERT combined with γ-irradiation on telomerase activity and DNA damage. Methods The recombinant expression plasmid pshRNA-hTERT was constructed and transfected into Hep-2 cells. The telomerase activity was examined by the PCR-hased telomeric repeat amplification protocol (TRAP). DNA single-stranded break (SSB) and the DNA double-stranded break (DSB) were detected by Comet assay. The xenograft model of human laryngeal carcinoma with the same genetic background and different radiosensitivity (Hep-2 and Hep-2R) was established in nude mice. The mixture of pshRNA-hTERT and liposome was injected to the transplanted tumor to observe the inhibition of the tumor growth. The cell apoptosis was detected by TUNEL. The hTERT protein expression was determined by streptavidin-peroxidase conjugated method (AP). Results Recombinant expression plasmid pshRNA-hTERT was successfully constructed and transfected into Hep-2 cells. The hTERT expression inhibition rate reached 60.78 %. pshRNA-hTERT not only inhibited telomerase activity of Hep-2 inehiding the increase of telomerase activity induced by γ-irradiation, but also inhibited the repair of the SSB and DSB induced by irradiation in the human laryngeal carcinoma xenograft in nude mice with the same genetic background and different radiosensitivity. The pshRNA-hTERT combined with γ-irradiation could inhibit the growth of transplanted tumor (Hep-2: EPO = 1.79; Hep-2R: EPO = 2.01) with reduced telomerase activity and hTERT protein expression. Conclusions The eukaryotic expression vector pshRNA-hTERT could enhance the radiosensitivity of Hep-2 cells in vitro and the human laryngeal carcinoma xenograft in nude mice which had the same genetic background with different radiosensitivity.

Objective To observe the characteristics offundus autofluorescence (FAF) in patients with polypoidal choroidal vasculopathy (PCV) before and after intravitreal ranibizumab injections.Methods A retrospective case series.Seventeen patients (17 eyes) including 11 males and 6 females were enrolled in this study.Best corrected visual acuity (BCVA),FAF and indocyanine green angiography examination were performed on all eyes.The eyes were divided into hypo-autofluorescence group (8 eyes) and mixed autofluorescence group (9 eyes) according to the fluorescence degree.There was no differences of BCVA between two groups (t=2.403,P=0.072).A11 eyes received monthly intravitreal ranibizumab injections for 3 months followed by an as-needed reinjection schedule.All eyes were followed up for 12 months.FAF was performed at the 3rd,6th and 12th month after first treatment.The changes of FAF characteristics and BCVA before and after treatment were observed.Results Before the treatment,the PCV lesions showed two distinct FAF patterns:the confluent hypo-autofluorescence at the polypoidal lesions and the granular hypo-autofluorescence at branching choroidal vascular networks (BVN).During the treatment,the abnormal FAF area of the whole lesions in all eyes reduced and gradually returned to normal.At the 3rd month after treatment,the central hypo-autofluorescence of polyps was surrounded by a hyper-autofluorescence ring,and with time,the ring was weakened or eliminated.However,all the hypo-autofluorescence findings in BVN at baseline were unchanged during the follow-up period.There was no significant differences in BCVA between hypo-autofluorescence group and hyper-autofluorescence group at different follow-up times (t=2.674,2.862,2.250;P=0.058,0.052,0.081).At final follow-up,5 eyes (62.5％) in hypo-autofluorescence group and 3 eyes (33.3％)in hyper-autofluorescence group had increased BCVA,the different was not significant (P=0.347).Conclusions Before the treatment,there were the central hypo-autofluorescence of polyps and circumferential hypo-autofluorescence ring or confluent hypo-autofluorescence.After the treatment,the autofluorescence of polyps increased and then gradually returned to normal.

Objective To establish the HPLC fingerprints of petroleum ether parts from five traditional medicine (Alpinia officinarum Hance, Alpinia galangal ( L.) Wild, Alpinia galanga Will., Alpinia katsumadai Hayata, and Alpinia oxyphylla Miq), and to explore the similarities and differences of chemical composition,as well as the correlation between the genetic relationship and the chemical composition. Methods HPLC method was used to analysis the five traditional medicines. The data were evaluated by using the"similarity evaluation system for chromatographic fingerprint of TCM" software. Results The similarity chemical composition from Alpinia officinarum Hance,Alpinia galangal(L.) Wild,Alpinia galanga Will.,Alpinia katsumadai Hayata,and Alpinia oxyphylla Miq in ethyl acetate were 0.741,0.855,0.610,0.510, 0.680,respectively. Conclusion Though there were differences of fingerprint peak of petroleum ether parts between five the traditional Chinese medicines, similarities were also observed among them.

This article has found that acupuncture can treat vascular cognitive impairment (VCI) through alleviating nerve cell apoptosis, enhancing hippocampal synaptic plasticity, improving hippocampal mitochondrial function, reducing brain inflammation, inhibiting oxidative stress response, protecting the blood-brain barrier, promoting angiogenesis, improving cerebral circulation, enhancing default network connectivity in brain regions, and improving white matter damage. At present, it is still in the preliminary stage to explore the mechanism of acupuncture treatment of VCI from the perspective of brain network connection. The existing mechanism researches mostly use electroacupuncture as an intervention method, and the selection of acupoints and the intensity of electroacupuncture stimulation are different. It is necessary to improve the rigor of the experiment, strengthen the study of conventional acupuncture, and explore the operation methods and needle retention time. The existing animal models lack consideration of other complications of VCI, and there are still some differences with the clinical characteristics of VCI patients, which need to be optimized in the future.

Objective:To explore the technical points and clinical effects of breast augmentation with silicone prosthesis through inframammary fold(IMF) incision.Methods:From January 2018 to June 2019, 26 cases of patients undergoing breast augmentation in our department were collected, including 10 cases of breast hypoplasia and 16 cases of breast atrophy, ranging in age from 24 to 38 years, with an average year of 30.2. The preselection of the volume, type of prosthesis and the design of new position of IMF were based on the height, weight, breast measurements and individual demand. During the operation, after precisely dissecting the lacunae, water were injected into the circular expander to reassess the volume of prosthesis, then the final prosthesis was implanted and the inframammary fold was reconstructed. After operation, patients were given analgesic pump and combined anti-cicatricial treatment. Postoperative complications, breast morphology, incision scar, and whether the patient was satisfied with the surgical evaluation were followed up.Results:All operations were successful and the operative time ranged from 38 to 45 minutes, the drainage time was 3-4 days, the drainage volume of the unilateral space was 30-50 ml, 96 percent of the patients (25/26) were satisfied with the incision. Postoperative following up lasted from 8 months to 16 months with an average of 12 months. No complications such as hematoma, infection or capsular contracture occurred. The breast shape of 26 patients after operation was natural, erect, plump and bilaterally symmetrical. The incision scar of new IMF was not conspicuous. The patients were very satisfied with the operation effect.Conclusions:Breast augmentation through IMF incision has advantages of short operating path, accurate dissecting and hemostasis under direct vision, short operation time. During the operation, in combination with re-evaluation after the expander inflated with saline to the required prosthesis volume and reconstruction of IMF, satisfactory clinical effects can be achieved with fewer complications.

Objective:To explore the technical points and clinical effects of breast augmentation with silicone prosthesis through inframammary fold(IMF) incision.Methods:From January 2018 to June 2019, 26 cases of patients undergoing breast augmentation in our department were collected, including 10 cases of breast hypoplasia and 16 cases of breast atrophy, ranging in age from 24 to 38 years, with an average year of 30.2. The preselection of the volume, type of prosthesis and the design of new position of IMF were based on the height, weight, breast measurements and individual demand. During the operation, after precisely dissecting the lacunae, water were injected into the circular expander to reassess the volume of prosthesis, then the final prosthesis was implanted and the inframammary fold was reconstructed. After operation, patients were given analgesic pump and combined anti-cicatricial treatment. Postoperative complications, breast morphology, incision scar, and whether the patient was satisfied with the surgical evaluation were followed up.Results:All operations were successful and the operative time ranged from 38 to 45 minutes, the drainage time was 3-4 days, the drainage volume of the unilateral space was 30-50 ml, 96 percent of the patients (25/26) were satisfied with the incision. Postoperative following up lasted from 8 months to 16 months with an average of 12 months. No complications such as hematoma, infection or capsular contracture occurred. The breast shape of 26 patients after operation was natural, erect, plump and bilaterally symmetrical. The incision scar of new IMF was not conspicuous. The patients were very satisfied with the operation effect.Conclusions:Breast augmentation through IMF incision has advantages of short operating path, accurate dissecting and hemostasis under direct vision, short operation time. During the operation, in combination with re-evaluation after the expander inflated with saline to the required prosthesis volume and reconstruction of IMF, satisfactory clinical effects can be achieved with fewer complications.