1.Remark on the Life Effect of Professional Ethics Education in Medicine
Chinese Medical Ethics 1994;0(05):-
According to the speciality of medical occupation and the form of professional ethics education (including its significance,content and method) received by medical personnel at school,this essay is to elaborate the life effect of professional ethics education in medical practice,which plays an important role in the improvement of public efficiency and economic profit,and the development of medical science in a healthy way.
2.Ethical Discussion On the Operation Agreement
Chinese Medical Ethics 1995;0(03):-
According to the law,signing the operation agreement between patient and hospital before operation is one of the most important parts of legal treatment.It discusses the importance of the effect,error regions in applying and modeling the operation agreement before operation.It discusses the function of operation agreement in protecting both patients' and medical staff's right,reducing medical disputes and strengthening the confidence of patients against diseases from the point of view of ethics.At the same time it analyses how to perfect and regulate the content and procedure of the agreement and have a good relationship between doctors and patients.
4.A Clinical Study on the Relationship between Maternal Blood Group Antibodies and the Incidence or Severity of HDN
Faqiang LU ; Jinghan LIU ; Xilin OUYANG
Journal of Chinese Physician 2001;0(10):-
0.05).⑵ With the increase of titers of anti-A(B) antibodies of IgG type, both the incidence and severity of HDN elevated (P
5.A Study on Trehalose Loading Technique of Human Platelets before Freezing-dried
Jun ZHOU ; Jinghan LIU ; Xilin OUYANG
Journal of Chinese Physician 2000;0(12):-
Objective To establish the technique of loading trehalose into human platelets including temperature, time and concentration of trehalose loading. Methods Intracellular trehalose concentration versus loading time curves at 37℃ and 16℃, mean plateletee volume(MPV) versus loading time curves, and loading concentration curves were investigated and compared. Then loading time and temperature for high loading efficiency of trehalose into human platelets were ascertained. Intracellular trehalose versus trehalose loading concentration curves were made, and loading efficiency was caculated. Results The trehalose loading efficiency of 4 hours at 37℃ was significantly higher than that at 16℃ (11.6% vs 5.6%). MPV at 16℃ increased 43.2% compared with at 37℃, but had no changes along with the change of loading time and concentration. When loading at 37℃, MPV change was positively related with loading time and concentration, and loading time and loading concentration had a cooperative effect. MPV had no changes in 1~4hours loading when trehalose loading concentration was under 50mM. MPV increased with the increase of loading time and concentration when trehalose loading concentration was above 50mM. When trehalose loading concentration was 45mM, loading efficiency at 37℃ reached 13.86% 4 hours aftr loading. Conclusion The basic parameter of trehalose loading technique of human platelets was loading temperature at 37℃, loading time for 4 hours, and loading concentration of 45mM.
6.Effects of loading trehalose into cytoplasm before freeze-drying on platelets activation and response to agonists in vitro
Faqiang LU ; Jinghan LIU ; Xilin OUYANG
Journal of Chinese Physician 2000;0(11):-
0.01).After incubation with trehalose for 4h,the expression of CD62p increased,and it decreased when platelets activation inhibitor PGE-1 and adenosine were added to the incubation buffer.The expression of PAC-1 showed no difference among the three groups. Conclusion The preprocessing before platelets lyophilization has no significant effects on the function and activity of platelets when the platelets activation inhibitor is added.
7.The feasibility of platelet long cryopreserved at-80℃
Jinghan LIU ; Xilin OUYANG ; Yuan ZHUANG
Chinese Journal of Blood Transfusion 1988;0(04):-
0.05).Conclusion Platelets maintain a good and stable quality at-80 ℃ and the-80 ℃ refrigerator may cryopreserve the platelets for a long time.
8.Research progress in stem cell transplantation for treating amyotrophic lateral sclerosis
Qiang LIU ; Xilin LU ; Xiaoli YAO
Chinese Journal of Tissue Engineering Research 2007;0(10):-
Amyotrophic lateral sclerosis is a progressive neurodegenerative disease that targets motor neurons without efficient treatment. Stem cell receives the enormous attention because it from specific tissues can differentiate into motor neuron. Stem cells from specific tissues could be used for amyotrophic lateral sclerosis, such as embryonic stem cells, neural stem cells, bone marrow mesenchymal stem cells, umbilical cord blood-derived stem cells and induced pluripotent stem cells. The mechanism of stem-cell therapy includes cell-replacement, delivery neurotrophic factors and immunomodulation. Animal researches and some clinical trails have confirmed that stem cells have great potential to treat neurodegenerative diseases. Till now, people are still unknown some aspects of stem cells, and many problems still need to be resolved.
9.EVALUATION OF PRESTORAGE LEUCODEPLETION IN MASSIVE BLOOD TRANSFUSION
Jinghan LIU ; Xilin OUYANG ; Ru LI
Medical Journal of Chinese People's Liberation Army 1981;0(06):-
The patients received massive blood transfusion in General Hospital of the Chinese Pepole's Liberation Army between 1999to 2001were reviewed. The following items of leucodepleted blood transfusion (LDBT)group and routing blood transfusion(RBT) group at pretransfusion, the first day, third day, and seventh day after blood transfusion were recorded and compared: glutamic pyruvic transaminase(GPT), glutamic oxaloacetic transaminase(GOT), lactate dehydrogenase(LDH), total bilirubin(TB), direct bilirubin(DB), alkalescence phosphorylase(ALP), total protein(TP), albumin(ALB), glucose (GLU), Ca 2+ , total CO 2, pH and febrile nonhemolytic transfusion reactions(FNHTR). The results showed that compared with RBT group, GPT in LDBT group on the third day after blood transfusion was significantly lower ( P 0 05). When antiallergic medicine was given, FNHTR rate of massive blood transfusion in RBT and LDBT groups was 27% and 1 9%( P
10.MULTIPLE ORGANINJURY BY HIGH CONCENTRATED PLATELET-LEUCOCYTE AGGREGATIONS IN RED BLOOD CELL CONCENTRATION AND ITS COUNTERMEASURE
Xilin OUYANG ; Jinghan LIU ; Nin HUANG
Medical Journal of Chinese People's Liberation Army 2001;0(12):-
To study the formation and change of platelet leucocyte aggregation (PLA) in red blood cell concentrations(CRCs) stored at 4℃ and the preventive effectiveness of filtration, 8 units of CRCs were each divided into two equal parts in two integrative bags and two paired groups were formed: control group and filtration group. The following items of control group and filtration group were detected weekly. Platelet and leukocyte counts, concentration and percentage of platelet lympocyte (P lym), platelet monocyte (P mon), platelet neutrophil (P neu) aggregatoins, and concentration of total PLA. The results showed that rate and concentration of PLA in the control group were significanly higher than those in the circulating blood. The rates of P lym and P mon in CRCs stored at 4 ℃ were two to five times of those before storage, and the rate of P neu in CRCs stored at 4 ℃ was ten to twenty times of that before storage. PLA in the CRCs separated from plasma rose to a high level at the first day of preparation. The concentration of PLA in CRCs reached the highest level at one week after stored at 4 ℃ and then decreased, but a high concentration was retained all the while. P neu is the major part (about 80%~90%) of whole PLA in CRCs. PLA was not detectable by flow cytometry during the whole storage course. The results suggested that high concentrated PLA was detected in CRCs stored at 4 ℃, which could be prevented by leucocyte depleting filtration.