A canthopanax Senticosus , a vulnerable species, was one of the major government-protected plants. This paper deals with the major reason for wildlife resources from biological standpoints, which are sexual propagation with less capability , vegetative propagation being damaged due to leaves , roots and rhizomes being gathered un-reasonable. Raising wildlife resources, semi-wild extensive management, and reasonable gatheration will be an important means for protection of wildlife resources in light of local conditions.

Objective To develop a method for simultaneous determination of 4 compounds in Zhizhu Pills by HPLC.Method Reversed-phase HPLC was used to determine the content of the compounds such as hesperidin,naringin,neohesperidin and atractylenolideⅠ.The column was symmetry C18(250 mm?4.6 mm,5 ?m) with temperature of 30 ℃.Methanol-0.095 %phosphoric acid solution served as the mobile phase by gradient elution.The wavelength of detection was set at 283 nm and 224 nm.The injection volume was 10 ?L and the flow rate was 1.0 mL/min.Results Hesperidin,naringin,neohesperidin and atractylenolideⅠin Zhizhu Pills can be isolated completely.Three batches of samples were determined and the recoveries of naringin,hesperidin,neohesperidin and atractylenolideⅠwere 98.81 %(RSD=1.11 %,n=6),100.63 %(RSD=1.90 %,n=6),99.32 %(RSD=1.44 %,n=6) and 99.65 %(RSD=1.53 %,n=6) respectively.Conclusion This method is accurate,reliable and with good reproducibility.It can be used for the quality control of Zhizhu Pills.

Objective:To elucidate the etiology of the impairment of myocardial cells in Keshan disease. Methods:With intracellular microelectrode technique, we observed the electrophysiological changes of the eight groups of rats fed separately:normal, low Se, low Se+high VC, low Cr, low Cr+high VC, low Se+low Cr and low Se+low Cr+high VC fodders. Results:Compared with the control, there was no change in low Cr group. However, in low Se group, RP and APA decreased, APD 50 and APD 90 prolonged. In low Se+low Cr group, APA decreased, APD 50 and APD 90 prolonged significantly. In low Se with high vitamin C groups, though RP still decreased, no change had been detected on APA and APD. Conclusion:It is suggested that Se and Cr deficiency may jointly change the electrophysiological properties of myocardial cells significantly. However, vitamin C may protect myocardial cells against the injury effectively.

Objective: To observe the effects of vitamin C(VC)on the electrophysiological property of myocardial cells of the rats fed with low selenium and high cadmium fodder. Method: Eight groups of rats were fed separately with: normal (2 groups), high Cd, high Cd+high VC, low Se+high Cd, low Se+high Cd+high VC, high Se+high Cd and high Se+high Cd+high VC fodder for 14 w. With intracellular microelectrode technique, the electrophysiological changes of rats were observed. Results:Compared with the control, there were no changes in high Cd+high VC group and in high Se+high Cd+high VC group. However,in high Cd group, RP and APA decreased, APD50 and APD90 prolonged; in low Se+high Cd group ,RP and APA decreased, APD50 and APD90 prolonged significantly; in low Se+high Cd+high VC group and high Se+high Cd group, though RP still decreased, no change had been detected on APA and APD. Conclusion: Se deficiency and simultaneous Cd overabundance may change the electrophysiological properties of myocardial cells of the rats significantly. However, VC may protect myocardial cells against the injury effectively.

The rehabilitation of Traditional Chinese Medicine (TCM) takes overall concept as its guiding ideology, including shape, qi and spirit. The core is to preserve and promote the inner overall vitality of the human body, and to overcome the dysfunction of human body through activating self ability of recovery. Based on this view, we integrated traditional and modern rehabilitation, developed the curriculum system and teachers.

OBJECTIVE: To investigate the meaning of the mutation screening, prevalence, inheritance and the intervention or the prevention for the specific drugs in 10 families with non-syndrome hearing loss in Yunnan Province, China. METHOD: To do a questionnaire about the cases of ten families with non-syndrome hearing loss and to draw a detailed matriarchal family tree detailed. Following that, the A1555G mutation-positive individuals were detected and confirmed using DNA extracting, PCR amplification and sequencing for family volunteer. RESULT: There are 96 members have attended the blood collection in these ten families. Thirty-six of them had the normal hearing and 60 of them had the sensory neural hearing loss. However, 4 out of those had no A1555G point mutation, and 92 had A1555G point mutation (95.8%). While 7 of those were Heterogeneity, the rest were all homogeneous mutation. There were also 73 patients who had amino glycoside antibiotic medication history. However all the rest cases had a history of amino glycoside antibiotic medication were not clear yet. CONCLUSION The proportion of patients with drug-induced deafness is high in Yunnan province and the mutation rate of mitochondrial DNA A1555G is also high. It is worthy to do DNA 12SrRNA A1555G mutation screening for drug intervention and prevention.
Adolescent ; Adult ; Aged ; Child ; China ; epidemiology ; DNA Mutational Analysis ; DNA, Mitochondrial ; genetics ; Deafness ; epidemiology ; genetics ; Female ; Humans ; Male ; Middle Aged ; Pedigree ; Point Mutation ; RNA, Ribosomal ; genetics ; Young Adult

OBJECTIVE: To study the clinical and sequence character of the entire mitochondrial genome in five subjects with mitochondrial 12SrRNA T1095C mutation, and to analyze its relationship with the military noise-induced hearing loss (NIHL). METHOD: Three hundreds and four soldiers exposed to military noise were selected in Yunan and Beijing, including susceptible (experimental) and tolerance (control) groups. Mitochondrial 12SrRNA T1095C mutation were found in 5 subjects. Then the complete nucleotide sequence of five subjects were sequenced and its clinical character were analyzed. RESULT: m12SrRNA T1095C mutation were identified in 5 subjects of experimental group,and none were found in control group. There was significant difference between them (P < 0.05). All five soldiers had the history of military noise exposure and showed sensorineural deafness of different degrees. Sequence analysis of the complete mitochondrial genomes showed the distinct sets of mtDNA polymorphism besides T1095C mutation in five subjects. CONCLUSION The T1095C mutation in hearing loss subjects with various genetic background and history of military noise exposure, is involved in the pathogenesis of hearing impairment. It indicates that the T1095C mutation do relate well with military noise induced-hearing loss.
Adult ; Base Sequence ; DNA, Mitochondrial ; genetics ; Hearing Loss, Noise-Induced ; genetics ; Humans ; Male ; Military Personnel ; Mutation ; Young Adult

The nasal swab samples of swine influenza(SI)suspected pigs were collected and tested for H3 subtype swine influenza virus(SIV)positive by RT-qPCR.The positive samples were inoc-ulated into SPF chicken embryos for virus isolation.The full genome sequencing and sequence anal-ysis of the isolated H3N2 subtype SIV were conducted,and its pathogenicity was studied.The re-sults showed that a strain of SIV was successfully isolated and identified as H3N2 subtype by RT-PCR,named A/Swine/Yunnan/KM/06/2023(H3N2).The BLSAT results showed that the eight segments of SIV H3N2 KM had the highest homology with eight different strains of swine influ-enza or human influenza viruses,reaching 95.41％-97.49％.The HA and NA segments were de-rived from H3N2 subtype SIV,the NP segment was derived from H1N1 subtype human influenza virus,the M segment was derived from H1N2 subtype SIV,and all other segments were derived from H1N1 subtype SIV.The key receptor sites(190D,223V,226I,228S)of HA protein remained unchanged.The pathogenicity experiment results showed that infected piglets exhibited symptoms such as fever,sneezing,runny nose,the virus could be detoxified to the outside through the nasal cavity,and the lungs had different degrees of lesion.Immunohistochemistry(IHC)showed that the virus could replicate in the lungs.In conclusion,a strain of H3N2 subtype SIV was successfully iso-lated,and the genetic evolution,molecular characteristics and pathogenicity of the virus were stud-ied.It revealed that H3N2 subtype SIV is constantly evolving and had pathogenicity to piglets,pro-viding a reference for monitoring and preventing SIV epidemics in China,and provided a candidate strain for SI vaccine development.