ObjectiveTo study the effect of interventional stent placement in patients of malignant obstructive jaundice(MOJ).MethodsTwenty eight MOJ patients were divided into three groups receiving endoscopic retrograde plastic stent placement, percutaneous transhepatic expandable stent placement (mental stent), and mental stent combined with intraarterial chemotherapy, respectively.Results Patent drainage lasted for over 3 mos in 60% of patients with plastic stent. The median survival was 4 5 months. In the group of mental stent placement drainage remained patent for over 10 months in 50% of patients, with a median survival of 6 5 months. Whereas in the group of combined intraarterial chemotherapy, 10 mos patency of the stent was 66 7% with a median survival of 8 8 months. ConclusionThe mental stent enables long patent drainage, and longer survival time.

Objective To analyze the epidemiological characteristics of annual reported people living with HIV/AIDS (HIV/AIDS) in Qingdao city,Shandong province from 2010 to 2014 and to provide theoretical evidence for strategies on targeted prevention and control.Methods Information on annual reported HIV/AIDS and sentinel surveillance data from 2010 to 2014 was abstracted from the HIV/AIDS Comprehensive Response Information Management System (CRIMS).Statistical analysis was done by SPSS software.Results The number of annual reported HIV/AIDS increased from 2010 to 2014 in Qingdao,with the sex ratio as 10.8.Among all the patients,77.1％ were aged 20-39 years,65.6％ were unmarried,67.6％ graduated from high school or above.Homosexual contact was the major mode of transmission,and the prevalence of MSM increased yearly (P＜0.05).Conclusions MSM appeared the major high risk population for AIDS epidemic in Qingdao.However,surveillance projects should also be strengthened among the female population with multiple sexual partners.Optimal prevention and control measures based on the characteristics of AIDS epidemic should be developed to explore the stem spread and epidemics of the disease,in Qingdao.

Epigenetic modification, especially histone modification, plays an important role in maintaining plant genome stability, regulating gene expression and promoting regeneration in vitro. MtSERK1 is an important marker gene involved in establishing of embryogenic callus during in vitro regeneration of Medicago truncatula. In order to understand the regulation Epigenetic modification, especially histone modification, plays an important role in maintaining plant genome stability, regulating gene expression and promoting regeneration in vitro. MtSERK1 is an important marker gene involved in establishing of embryogenic callus during in vitro regeneration of Medicago truncatula. In order to understand the regulation relationship between dynamic histone modification and MtSERK1s expression during the processes of in vitro organogenesis, the expression of MtSERK1 was analyzed by qRT-PCR, and the modification status of H3K9me2, H3K4me3 and H3K9ac in the promoter region and different regions included in the gene body was analyzed by chromatin immunoprecipitation (ChIP). We found expression activation of MtSERK1 was related to the dynamic changes of histone H3K4me3 and H3K9ac in the 5' and 3' regions. This study will provide important theoretical guidance for understanding of the regulatory mechanism of MtSERK1 and also for establishing efficient genetic transformation system of Medicago truncatula.
Epigenesis, Genetic ; Gene Expression Regulation, Plant ; Genome, Plant ; Histone Code ; Medicago truncatula ; genetics ; growth & development ; Protein Kinases ; genetics ; Regeneration