Objective:To observe the transfection efficacy and targeting efficiency of hepatitis B virus envelope(HBVE) as a gene transfer vector for liver cancer cells.Methods:HBVE was obtained from the supernatant of HepG2.2.15 cells with a PEG8000 system and ?-propiolactone.The pIRS2-EGFP was packed with HBVE to obtain HBVE-GFP and was packed with liposome to obtain Liposome-GFP.HBVE-GFP and Liposome-GFP were used to transfect human hepatoblastoma cell line HepG 2 to study the transfection efficiency.HepG 2,A 549,HeLa and FB cells were transfected with HBVE-GFP to appraise the targeting ability of HBVE-GFP.GFP protein expression was observed under a fluorescent microscope and the ratio of GFP positive cells was determined by flow cytometry.Results:(1) Transfection efficiency:The GFP protein was observed in both the liposome group and the HBVE group under the fluorescent microscope;the fluorescent intensity in the HBVE group was 3-4 times that of liposome group as determined by flow cytometry(P

Objective To explore the influence of the health belief model education on the life quality of nurses.Methods 200 nurses were randomly divided into the experimental group and the control group with 100 cases in each,the experimental group received health belief model education while the control group was given no intervention.The life quality of nurses before and after the education was compared.Results The life guality of nurses showed no significant difference between the two groups before the health belief model education,one month after health belief model education,life quality of nurses in the two groups showed significant difference.Conclusions Application of the health belief model education can improve the nurses'quality of life.

Objective To investigate the clinical effect and Practical value of percutaneous vertebroplasty using China-made bone cement. Methods Under CT or C-arm X-ray machine guided, 22 cases with vertebral osteoporotic compression fracture, hemangeoma orosteolytic carcinoma were treated by perfusion of China-made bone cement through percutaneous puncture. Results Thirty vertebraes in 22 cases were operated by percutaneous vertebroplasty and the successful rate was 100%. The symptoms in 21 cases were remarkably released, the clinical effective rate was 95.5%. During the operation, bone cement leakage occurred in 23.3% of the treated vertebrae, but no serious untoward effect appeared. Conclusion The application of China-made bone cement in percutaneous vertebroplasty is of the features of minimal invasion, safty and remarkable curative effect. It can release pain and consolidate the vertebrae.

Humans ; Child ; Risk Factors ; Adipose Tissue