1.The Immunomodulatory Effects of Anisodamine on Rabbits with Acute Experimental Serum Sickness
Journal of Third Military Medical University 1983;0(03):-
Anisodamine (654-2) was administered to rabbits with acute experimental serum sickness (AESS) to study the immunomodulatory effects of 654-2 on AESS. The results suggested that 654-2 may decrease the serum specific antibody and immune complex (IC), inhibit the activity of serum cathepsin D (Cath-D), make the level of complemnts in narrow change, and improved the renal injury.
2.Effects of Liposome-encapsulated Nitrogen Mustard on Acute Experimental Serum Sickness in Rabbits
Journal of Third Military Medical University 1988;0(05):-
Nitrogen mustard encapsulated with liposomes was administered to the rabbits with acute experimental serum sickness(AESS). Then the serum levels of anti-BSA antibodies and circulating; immune complex(CIC), CH50, and WBC count were determined. The ACPase activity of spleen macrophages was measured. And the sections of renal tissues, after HE-stained, were studied for the accumulation of CIC and the pathological changes. It was found that liposome-encapsulated nitrogen mustard can increase the macrophage activity and in turn aggravate the renal damages of AESS rabbits.The results suggest that the renal damages of AESS rabbits can immunopatholo-gically be attributed to the increased activity of macrophages.
3.The Regulatory Effects of Dexamethasone on the Acute Experimental Serum Sickness in Rabbits
Journal of Third Military Medical University 1983;0(03):-
This experiment takes the apute experimental serum sickness (AESS) in rabbits as an animal model to investigate the mechanism of the regulatory effects of Dexamethasone (DX) . The results show. DX doesn't inhibit the production of anti-BSA antibody but induces the tendency to increase the level of antibody in rabbits with DX administration on early days (EL, EH); immune complexes(BSA - anti-BSA IGg, CIC) in the rabbits of EL and EH are significantly increased comparing with the later days (LE, LH) and positive controls. Non-specific esterase stainings and the pathological changes of kidney sections are markedly lessened though the amount of anti - BSA antibody, circulating immune complex (CIC) and superoxides dismutase activity in the rabbits of LE and L H are the same as the positive control. The outcome of protecting kidneys of AESS rabbits from injury by DX administration . probadly results from the effects of that DX inhibits infiltration of monocytes into the glomeruli. The role of oxygen radicals in AESS needs further investigation.
4.Immunopharmacologicat Effects of Anisodamine on Immune Complex Glomerulonephritis in Rabbits
Chinese Journal of Immunology 1985;0(05):-
The immunopharmacological effects of anisodamine(654-2)on immune complexnephritis were examined in rabbits with acute experimental serum sickness(AESS).The results showed that activities of renal calmodulin(CaM)and cathepsin D(Cath-D)were significantly high.654-2 could inhibit CaM activity and immune complex-induced lysosomal enzyme release,and lessen immune complex glomerulonephritis at thesame time.Our findings suggest that a marked inhibition of CaM activity by 654-2 mi-ght contribute greatly to the improvement of immune complex glomerulonephritis.
5.Effect of enamel matrix proteins on the growth of apatite coating on dual thermo-etching modified titanium
Xihua ZHU ; Qianwen WU ; Hui HUANG
Chinese Journal of Tissue Engineering Research 2017;21(2):249-253
BACKGROUND:Various surface modification techniques have been used to improve the bioactivity of titaniumimplant in vivo. OBJECTIVE:To investigate the effects of enamel matrix proteins (EMPs) on the growth of apatite coatings on dual thermo-etching treated pure titanium. METHODS:EMPs were extracted from porcine tooth germs and then were identified. Dual thermo-etching was applied to treat titanium samples fol owing polished, and then immersed in a blank simulated body fluid supersaturated calcification solution (control group) or supersaturated calcification solution containing different concentrations of EMPs for 7 days. The morphology of samples was observed using scanning electron microscope, and element components and crystal structures of the apatite coatings were analyzed by energy dispersive spectrometer and X-ray diffraction. RESULTS AND METHODS:After double-etching, a pit-like rough surface was observed on the titanium plate. After 7-day mineralization, in the control group, no overt calcium-phosphate deposits were found on the titanium surface;however, in the experimental groups, there were calcium-phosphate deposits, whose quantity and morphology changed with increasing concentrations. Energy dispersive spectrometer showed that the main element components of the mineralized coating included calcium, phosphorus, oxygen and carbon, and the calcium-phosphate ratio ranged from 1.32 to 1.41. The apatite coatings were proved to be carbonate hydroxyapatite by X-ray diffraction. To conclude, EMPs promote apatited deposition on pure titanium surfaces in a concentration-dependent manner.
6.Enzyme-linked Immunosorbent Assay to Determine Specific Immune Complex in Rabbit circulation
Feisha ZHAO ; Jianping WANG ; Xihua ZHU
Journal of Third Military Medical University 1983;0(03):-
A method, enzyme-linked immunosorbent assay, was introduced for the determination of antigen-specific immune complex (BSA-anti-BSA) in rabbit circulation. The antigen-specific immune complex in the circulation of twelve rabbits were determined by this method. The results showed that this method accords with the regular pattern of immune complex formation more closely than the antigen-nonspecific assay, PEG precipitation method. The procedure of this method is rather simple, its specificity is of high degree, and the amount of serum needed in test is small. This enzyme-linked immunosorbent assay is a better method to determine the circulating specific immune complex in rabbits.
7.Expression of the extracellular domain of human CD7 in Escherichia coli
Yanchun ZHOU ; Xihua ZHU ; Yunhui HUANG
Immunological Journal 2001;(2):105-106,109
Objective To get the antigen of CD7.Methods The extracellular domain of human CD7 was cloned from a plasmid containing the full length of human CD7 cDNA and expressed in pinpoint-xa3 prokaryotic system. Results Analysis with SDS-PAGE and Western-blotting showed that the expressed protein could bind to the anti-CD7 mAb specifically and is about 30 000 u in molecular weight. Conclusion These results paved the way for preparing anti-CD7 engineering antibody.
8.Study of activation of human T cells with superantigen SEA
Guilian XU ; Xihua ZHU ; Jin YANG
Immunological Journal 2001;(2):85-87,104
Objective To investigate the activation of human T cells with superantigen SEA. Methods T cells proliferation, IL-2 production, DNA synthesis, cell phenotype and apoptosis induced by the SEA in the first or restimulation were detected. Results It was shown that 100 ng/mL was the optimal activation concentration, IL-2 production arrived at top level in the third day, SEA activated CD4+ and CD8+T with the same degree, T cells start apoptosis in response to SEA restimulation within 24 hours and apoptosis disappeared through addition of rhIL-2. Conclusions There were correlation between activation and SEA concentration or stimulation period; SEA activated both CD4+ and CD8+T cells without change the cell phenotype.
9.Molecular design for antagonisting C5a anaphylatoxin and the activity examination of the peptide
Fenglin LV ; Xihua ZHU ; Ping ZHENG
Chinese Journal of Immunology 1985;0(03):-
Objective:To discover some high hydrophilic profiles of the human C5a anaphylatoxin based on relationship between the structure and function of the protein and the protein molecular design principles.Methods:The peptides were synthesized by 431A automatic peptide synthesizer,purified by PHLC and confirmed by caplilliary electrophoresis.Results:The N terminus No.9 30 profile of the C5aR(P22) could interacte with anti C5aR McAb(S5/1,from Serotic Co.),as determined by ELISA.Furthermore,it could be inhibited OD490 values remarkably by 10.0 ?g/L rhC5a(P
10.Construction and identification of expression of superantigen SED mutants
Yafei LI ; Xihua ZHU ; Yunhui HUANG ; Jin YANG ;
Journal of Third Military Medical University 1984;0(01):-
Objective To construct staphylococcal enterotoxin D (SED) mutants expressed in Escherichia coli ( E. coli ). Methods The expected mutants were introduced into the SED DNA by megaprimer PCR method. The PCR products ligated to plasmid pTrcHis B were transformed into E. coli DH5? for IPTG induced expression. The target protein was purified by Ni NTA metal affinity chromatography and analyzed by SDS PAGE and immunoblotting. Results The sequencing results showed that mutant nucleic acids were successfully introduced at the expected sites of SED gene. SDS PAGE and immunoblotting confirmed that the proteins of SED mutants were obtained by Ni NTA metal affinity chromatography. The mutants were named as SEDN23A, SEDN23A/H26R, SEDF45A, SEDL59A, SEDN61A, SEDI92A, and SEDF203A, respectively. Conclusion Several SED mutants are successfully constructed, which lays a foundation for subsequent studies of immune recognition of SED.