Objective To establish a method of automatic analysis of iodide in drinking water.Methods In accordance with the principles of the National Standard Methods Standard Testing Methods for Dringking Water (GB 5750-2006),the reagent concentration and the ratio of dosage were optimized; the national standard artificial colorimetric detection of iodide in drinking water was substituted by automatic biochemical analyzer colorimetric analysis procedure; the accuracy and the precision of the new method were observed and compared with the National Standard Methods.Results In determination of iodide in drinking water by using automatic biochemical analyzer,the best linear ranges of the standard curve:low concentrations 0-＜ 50 μg/L with a correlation coefficient of 0.999 1,and the detection limit was 0.4 μg/L; at high concentrations of 50-500 μg/L the correlation coefficient was 0.999 3,and the detection limit was 4.0 μg/L.The coefficient of variation was 2.2％-4.2％ and the recovery was between 98.6％-1035％.Compared with the National Standard Methods,the difference was not statistically significant (t =0.97-1.70,all P ＞ 0.05).Conclusions The method of determination of iodide in drinking water by using automatic biochemical analyzer is successfully established.The accuracy and the precision of the method can meet the quality requirements of water quality analysis.