Objective]This article discussed Professor Xie Jianguo's survival with tumor experience on advanced lung cancer treatment in the last decade. [Methods]To observe Professor Xie Jianguo's survival with tumor experience of advanced lung cancer on the clinical treatment process, and organize related cases, which summarized the unique thoughts of treatment, commonly used empirical prescriptions and demonstrated with typical clinical cases. [Resluts] Professor Xie Jianguo's theoretical guidance on advanced lung cancer treatment, included tumor inhibition, anticancer effect, survival with tumor. Treatment methods, included recruitment of the vital qi, adjusting the internal environment, the phlegm, stasis toxin factors, with rigorous thinking, sophisticated medication. [Conclusion] Professor Xie Jianguo's survival with tumor experience on advanced lung cancer treatment has remarkable significance in guiding clinical treatment.

Objective To observe the clinical efficacy of qi-based acupuncture treatment in treating chronic gastritis due to liver-stomach disharmony. Methods Totally 120 patients with chronic gastritis due to liver-stomach disharmony were randomized into group A, group B, and group C, 40 in each group. Group A was intervened by qi-based acupuncture treatment; group B was by medication;group C was by sham acupuncture by selecting points 1 cm away from the points selected in group A. The therapeutic efficacies were evaluated after 4-week treatment. Results The total effective rate was 95.0%in group A, versus 75.0%in group B and 45.0% in group C, and the total effective rates in group A and B were significantly different from that in group C (P<0.01, P<0.05). The total effective rate in group A was significantly different from that in group B (P<0.05). Conclusions Qi-based acupuncture treatment is an effective approach in treating chronic gastritis due to liver-stomach disharmony.

Objective To investigate the clinical efficacy of combined use of acupuncture and medicine in treating functional gastric acid regurgitation. Methods Ninety-eight patients with functional gastric acid regurgitation were randomly allocated to treatment and control groups, 49 cases each. The control group received routine medication and the treatment group, acupuncture plus Chinese herbal medication in addition. The symptom severity and frequency were scored in the two groups before and after treatment. The clinical therapeutic effects were compared between the two groups. Results There were statistically significant post-treatment differences in the symptom severity score and the symptom frequency score between the treatment and control groups (P＜0.05). The total efficacy rate was 95.9% in the treatment group and 83.7% in the control group; there was a statistically significant difference between the two groups (P＜0.05). Conclusion Combined use of acupuncture and medicine is an effective way to treat functional gastric acid regurgitation.

Objective To study the efficacy and safety of intracavitary injection of endostar combined with chemotherapy of malignant pleural effusion.Methods 46 cases with malignant pleural effusion were divided into groups of intracavitary injection of endostar combined with chemotherapy of malignant pleural effusion (treatment group)and groups of intracavitary chemotherpy(control group)randomly,pleural effusion of the patients from the two groups were injected cisplatin(DDP)60mg into the thorax and 45mg of endostar,The same therapy applied to the control group except adding endostar,twice a week,in 2 -4 rounds,the efficacy and safety of the two groups were studied.Results The effective rate of the treatment groups was 58.3%,which was higher than 31.8%of the control group (χ2 =4.547,P<0.05).The improvement rate of quality of life was 75.0% in the treatment group,which was higher than 59.1% in the control group (χ2 =5.371,P<0.05).The toxicities of the treatment group were not increased significantly.Conclusion The therapy of intracavitary injection of endostar combined with chemotherapy of malignant pleural effusion is effective and secure.

The present paper has reviewed and researched on 112 gynecological records from volume 17 ofZhang-Yuqing’s medical records, and has found that there were 9 common methods of treating gynecological diseases, namely dredging and regulatingqi and blood, adjusting qi to nourish ying, soothing the liver wood, harmonizing the liver and spleen (stomach), tonifying spleen and kidney, nourishing liver and kidney, regulating vital and conception vessels, eliminating dampness and phlegm, and nourishing Yin and clearing away heat.The treatment of gynecological diseases reflects the holistic concept and dialectical concept, and the valuable experience still has a high theoretical value, as well as clinical value, which is worth exploring and discussing.

OBJECTIVE To investigate the apoptosis of hepatocytes in arsenic poisoning rats caused by coal-burning and explore the effects of p53-induced mitochondrial apoptotic pathway on arsenic liver injury.METHODS Wistar rats were fed with 164.74 pp m arsenic conta minated grain at the levels of 15%,30% and 60% (arsenic contents were 25,50 and 100 mg·kg -1 ,respectively)for 90 d. The arsenic contents of urine and hair,apoptosis of hepatocytes and mRNA expression of p53,Bax and Bcl2 in peripheral blood and hepatocytes were evaluated.At the sa me ti me,protein expression of p53, Bax and Bcl2 in hepatocytes were analyzed.RESULTS The arsenic contents of urine and hair increased with the elevation of arsenic dose.The apoptotic rate of hepatocytes in arsenic 50 and 100 mg·kg -1 group were 16.49 ±2.06 and 15.83 ±1 .28,respectively which were significantly higher than the control group and arsenic 25 mg·kg -1 group (9.00 ±0.59 and 9.27 ±0.36,respectively,P <0.05).p53 mRNA expression of peripheral blood in arsenic 100 mg·kg -1 group was 2.69 ±1 .84 while p53 mRNA expression of hepatocytes in arsenic 25,50 and 100 mg·kg -1 group were 1 .63 ±0.28, 1 .91 ±0.38 and 1 .71 ±0.18,respectively which were significantly higher than the control group (0.86 ± 0.15 and 1 .22 ±0.12,respectively,P<0.05).Bax mRNA expression of peripheral blood in arsenic 50 and 100 mg·kg -1 group were 1 .36 ±0.30 and 1 .94 ±0.65 while Bax mRNA expression of hepatocytes in arsenic 100 mg·kg -1 group was 1 .34 ±0.23 which were significantly higher than the control group (0.77 ±0.15 and 0.84 ±0.34,respectively,P<0.05).Bcl2 mRNA expression of hepatocytes in arse-nic 100 mg·kg -1 group was 0.98 ±0.50 which was significantly lower than the control group (2.14 ± 1 .15,P<0.05).p53 protein expression of hepatocytes in arsenic 25,50 and 100 mg·kg -1 group were 1 .06 ±0.56,1 .15 ±0.77 and 0.74 ±0.27,respectively while Bax protein expression of hepatocytes in arsenic 50 and 100 mg·kg -1 group were 0.74 ±0.43 and 0.69 ±0.37 which were significantly higher than the control group (0.36 ±0.1 9 and 0.25 ±0.09,respectively,P<0.05).CONCLUSION Arsenic can induce hepatocytes apoptosis and p53-mediated mitochondrial apoptotic pathway may be involved in the develop ment of rat liver injury in arsenic poisoning rats caused by coal-burning.

Objective To explore the clinical effect of treating the ischemia of vertebral-basilar arteries by venous infusion of Yinxingdamo.Methods 116 cases of the patients suffering from the ischemia of vertebral-basilar arteries were divided randomly into two groups.In study group,66 patients were infused 20ml of Yinxingdamo injection added in 0.9% NS 250ml by I.V. q.d. for two weeks.In controlled group 50 patients were infused 20ml of compound danshen injection in 250ml 0.9% NS by I.V q.d. for two weeks.Results In study group,the symptoms of 55 cases were completely remissive,6 cases significantly improved,4 cases mildly improved,and no improvement was found in one case.In controlled group,the symptoms of 5 cases were completely remissive,18 cases significantly improved,16 cases mildly improved,and no improvement was found in 11 cases.In study group the total efficacy rate of 98.48% was significantly higher than that of controlled group which was 78%(P

Full ceramic restorations become more and more popular for their excellent properties. Bond strength between ceramic and composite resin cement is the key factor to success in clinical use. This paper illuminated the importance of ceramic surface treatments and reviewed recent progresses on ceramic surface treatments. The difference of bond strength caused by different dental materials was discussed too.

Objective To investigate the whole differentiating process of myogenic satellite cells in vitro,and the expression of N-ACh receptors.Methods Skeletal muscle satellite cells were isolated by trypsin digestion in vitro and purified by preferential attachment method.The cells were observed morphologically and identified by ?-sarcomeric actin,desmin and skeletal myosin antibodies.Contraction of myotubes was observed after adding ACh in the culture medium, N-AChRs in myotube were also be identified by bungarotoxin.Results Cultured satellite cells in vitro differentiated into myoblasts and fused to form myotubes later.These cells were positive by ?-sarcomeric actin, skeletal myosin and desmin antibodies' staining.Adding ACh directly in the medium stimulated the contraction of myotubes.No obvious expression of ACh receptors was observed on the surface of satellite cells.Cluster of receptors aggregated on myotubes from neonatal rats' satellite cells cultured in differentiation media.Conclusion The myotubes differentiated from myogenic satellite cells have the function of contraction.N-ACh receptors were synthesized gradually during the process of differentiation.

Objective In previous experiment we proved that rat marrow stromal cells(MSCs) could be induced to differentiate to neuron\|like cells by ? mercaptoethanol and all\|trans\|retinoic acid(ATRA), but the neuron\|like cells showed apoptosis sooner after the differentiation.In this study, we used several sets of combinations of anti\|apoptotic reagents and inductive reagents to induce the differentiation of MSCs,to prevent the differentiated cells from apoptosis and to elongate the lifetime of the neuron\|like cells which might be use in clinic. Methods The MSCs were isolated primarily from bone marrow of adult rat,and purified by passage culture.The 5th passage of MSCs was induced by single or combinative induction,which included RA,Melatonin(10 -9 mol/L), bFGF,bFGF+RA,Melatonin+RA,Melatonin+bFGF.At 1?h,5?h and 24?h of induction, the ratio of neuron\|like cells and the numbers of survived neuron\|like cells among 6 groups were compared.In addition,immunocytochemical stainings with anti\|MAP\|2 and GFAP were also performed on cells of 5?h of induction. Results Neuron\|like cells were all MAP\|2 positive,but GFAP negative.The ratios of neuron\|like cells in MSCs at 1?h after induced by six combinations of reagents were 0 24,0 28,0,0,0 44,0 64;at 5?h were 0 56,0 28,0,0,0 76,0 83 and at 24?h were 0 10,0 27,0,0 14,0 35,0 59 respectively.The ratios of survived neuron\|like cells of differentiated cells at 24?h after induction by RA,Melatonin,Melatonin+RA and Melatonin+bFGF were 0 08,0 37,0 05,0 51 respectively.Conclusions\ 1.bFGF can't induce MSCs into neuron\|like cells independently.Moreover,it suppressed the differentiation effects of RA,while enhancing the effects of MT.2.MT can induce MSCs differentiating into neuron\|like cells and has the anti\|apoptotic effects on the induced neuron\|like cells.3.When MT and bFGF were added together,not only the ratio of neuron\|like cell in MSCs was increased, the lifetime of neuron\|like cells was also elongated.