OBJECTIVE To investigate the apoptosis of hepatocytes in arsenic poisoning rats caused by coal-burning and explore the effects of p53-induced mitochondrial apoptotic pathway on arsenic liver injury.METHODS Wistar rats were fed with 164.74 pp m arsenic conta minated grain at the levels of 15%,30% and 60% (arsenic contents were 25,50 and 100 mg·kg -1 ,respectively)for 90 d. The arsenic contents of urine and hair,apoptosis of hepatocytes and mRNA expression of p53,Bax and Bcl2 in peripheral blood and hepatocytes were evaluated.At the sa me ti me,protein expression of p53, Bax and Bcl2 in hepatocytes were analyzed.RESULTS The arsenic contents of urine and hair increased with the elevation of arsenic dose.The apoptotic rate of hepatocytes in arsenic 50 and 100 mg·kg -1 group were 16.49 ±2.06 and 15.83 ±1 .28,respectively which were significantly higher than the control group and arsenic 25 mg·kg -1 group (9.00 ±0.59 and 9.27 ±0.36,respectively,P <0.05).p53 mRNA expression of peripheral blood in arsenic 100 mg·kg -1 group was 2.69 ±1 .84 while p53 mRNA expression of hepatocytes in arsenic 25,50 and 100 mg·kg -1 group were 1 .63 ±0.28, 1 .91 ±0.38 and 1 .71 ±0.18,respectively which were significantly higher than the control group (0.86 ± 0.15 and 1 .22 ±0.12,respectively,P<0.05).Bax mRNA expression of peripheral blood in arsenic 50 and 100 mg·kg -1 group were 1 .36 ±0.30 and 1 .94 ±0.65 while Bax mRNA expression of hepatocytes in arsenic 100 mg·kg -1 group was 1 .34 ±0.23 which were significantly higher than the control group (0.77 ±0.15 and 0.84 ±0.34,respectively,P<0.05).Bcl2 mRNA expression of hepatocytes in arse-nic 100 mg·kg -1 group was 0.98 ±0.50 which was significantly lower than the control group (2.14 ± 1 .15,P<0.05).p53 protein expression of hepatocytes in arsenic 25,50 and 100 mg·kg -1 group were 1 .06 ±0.56,1 .15 ±0.77 and 0.74 ±0.27,respectively while Bax protein expression of hepatocytes in arsenic 50 and 100 mg·kg -1 group were 0.74 ±0.43 and 0.69 ±0.37 which were significantly higher than the control group (0.36 ±0.1 9 and 0.25 ±0.09,respectively,P<0.05).CONCLUSION Arsenic can induce hepatocytes apoptosis and p53-mediated mitochondrial apoptotic pathway may be involved in the develop ment of rat liver injury in arsenic poisoning rats caused by coal-burning.

Objective To investigate the association between Down's syndrome(DS) and congenital heart diseases(CHD).Methods A total of 575 cases with DS from Jan.1997 to Mar.2013 in Children's Hospital of Chongqing Medical University were recruited.Retrospective study was conducted to analyze the prevalence and types of CHD in DS children,the relationship between the karyotype of DS and the types of CHD,and pulmonary hypertension (PH) and operation treatment.Results There were 370 cases(64.35％,370/575 cases) with CHD in 575 cases with DS.Among the 370 cases of CHD,322 cases (87.03 ％) were septal defects.In which,57 cases (15.41％) were atrial septal defects,36 cases (9.72％) were ventricular septal defects,12 cases (3.24％) were atrioventricular septal defects,and 157 cases(47.30％) were complex septal defects.Forty-eight cases(12.97％,48/370 cases) were nonseptal defect types of CHD (including patent ductus arteriosus,tetralogy of Fallot,double outlet right ventricle,pulmonary atresia,and so on).There was no statistical significance between the karyotype of DS and the types of CHD.There were 246 cases(66.49％,246/370 cases) with PH.Seventy cases(18.92％,70/370 cases) had interventional or surgical operations.All of them had descending pulmonary artery pressure after operation.Forty cases had other malformations such as gastrointestinal tract malformation,polydactylism / polydactyly,visual impairment,and so on.Conclusions The most common type of CHD with DS was atrial septal defect,and the second one was ventricular septal defect.There was no relationship between the karyotype of DS and the types of CHD.The patients with CHD in DS were prone to develop PH.So the comprehensive treatment plan should be developed as early as possible.

Triple-negative breast cancer ( TNBC ) is a subtype of breast cancer,and it is characterized by an aggressive clinical course with a poor prognosis.Treatment for TNBC has attracted much attention in recent years,however,there is no standard treatment for TNBC in clinical setting.The pCR rates of neoadjuvant chemotherapy in TNBC ranges from 12％ to 48％ in the current published data,and it is higher than that in other types of breast cancer,however,the fluctuating range of the TNBC ’s pCR is large in literature.Although the administration of adjuvant chemotherapy for early TNBC is controversial,the regimen without anthracyclines is reported to be suitable for early TNBC patients.Standard cytotoxic agents including taxanes and anthracyclines are still the main choices for TNBC salvage treatment,and the combination with gemcitabine or capecitabine may improve the overall survival.Poly (ADP-ribose) polymerase (PARP) is a new molecular target for TNBC in ongoing studies.Further research on the target-inhibitors such as BSI-201and Olaparib will provide more effective choices to clinical treatment.

Objective To explore the mediation effect of self-esteem on the relationship between perfectionism and depression in college students.Methods Totally 292 college students completed Frost Multidimensional Perfectionism Scale (FMPS),Hewitt Multidimensional Perfectionism Scale (HMPS),Slaney Almost Perfect Scale-Revised (APS-R),Rosenberg Self-esteem Scale (SES) and Beck Depression Inventory (BDI).Results Maladaptive perfectionism was positively correlated with depression (r =0.54,P ＜ 0.01),and negatively correlated with self-esteem (r =-0.55,P ＜ 0.01).Adaptive perfectionism was not correlated with depression (r =-0.09,P ＞ 0.05),and was positively correlated with self-esteem (r =0.36,P ＜0.01).Self-esteem partially mediated the association between maladaptive perfectionism and depression.The partial mediation effect was significant (P ＜0.05).The mediation model fitted the data well (GFI =0.986,CFI =0.988,NFI =0.981,TLI =0.970,RMSEA =0.075).Conclusion Self-esteem has partially mediating effect on perfectionism and depression.

Objective To analyze the correlation between pressure ulcer during operation and operation time under lateral operation position.Methods 89 operation patients all adopted lateral position.Pressure ulcer during operation was observed and operation time was registered.Results The operations went on smoothly.Operation time lasted 1 to 8 hours.There were 42 operations exceeded 4 hours and pressure ulcer during operation was observed in 15 patients.Positive rate was 35.7％.There were 47 operations lasted less than 4 hours and pressure ulcer during operation was observed in 6 patients.Positive rate was 12.8％.Statistical differences were significant between the two groups.Conclusions For those operation patients with lateral position whose operation time would be longer,especially more than 4 hours,nursing intervention measures should be adopted to prevent pressure ulcer during operation.

The respiratory syncytial virus (RSV) belongs to the family Paramyxoviridae and subfamily Pneumovirinae. The RSV can cause acute infections of the lower respiratory tract in infants. The F gene of the RSV is a conservative gene and varies only slightly in its expression. Few studies focusing on the variability of the F gene have been carried out. F protein (fusion glycoprotein) is a transmembrane glycoprotein that mediates fusion and penetration between the virus and host cells. Neutralizing antibody against the F protein can protect against infection by RSV subtypes A and B. Hence, F protein has become the main target for the development of a monoclonal antibody and vaccine against the RSV. An effective vaccine is not available, so a monoclonal antibody against F protein is now the most important method to reduce the morbidity and severity associated with RSV infection in high-risk children. However, a monoclonal antibody can lead to the production of drug-resistant strains of the RSV. This review focuses on genetic variation of the F gene of the RSV as well as progress in the development of a monoclonal antibody against F protein and a vaccine in the last decade.
Animals ; Antibodies, Monoclonal ; immunology ; Humans ; Respiratory Syncytial Virus Infections ; immunology ; prevention & control ; virology ; Respiratory Syncytial Viruses ; genetics ; immunology ; Viral Fusion Proteins ; genetics ; immunology ; Viral Vaccines ; genetics ; immunology

Autophagy is associated with the occurrence,development and drug resistance of ovarian cancer,which has become a hot topic in ovarian cancer.Many scholars believe that cancer is closely associated with tumor suppressor genes,and some tumor suppressor genes are related to autophagy.This article will review the effects of tumor suppressor genes on ovarian cancer through autophagy at home and abroad,which provides the reference for clinical research.

Objective To study the efficacy and safety of intracavitary injection of endostar combined with chemotherapy of malignant pleural effusion.Methods 46 cases with malignant pleural effusion were divided into groups of intracavitary injection of endostar combined with chemotherapy of malignant pleural effusion (treatment group)and groups of intracavitary chemotherpy(control group)randomly,pleural effusion of the patients from the two groups were injected cisplatin(DDP)60mg into the thorax and 45mg of endostar,The same therapy applied to the control group except adding endostar,twice a week,in 2 -4 rounds,the efficacy and safety of the two groups were studied.Results The effective rate of the treatment groups was 58.3%,which was higher than 31.8%of the control group (χ2 =4.547,P<0.05).The improvement rate of quality of life was 75.0% in the treatment group,which was higher than 59.1% in the control group (χ2 =5.371,P<0.05).The toxicities of the treatment group were not increased significantly.Conclusion The therapy of intracavitary injection of endostar combined with chemotherapy of malignant pleural effusion is effective and secure.

Objective To investigate the clinical efficacy of combined use of acupuncture and medicine in treating functional gastric acid regurgitation. Methods Ninety-eight patients with functional gastric acid regurgitation were randomly allocated to treatment and control groups, 49 cases each. The control group received routine medication and the treatment group, acupuncture plus Chinese herbal medication in addition. The symptom severity and frequency were scored in the two groups before and after treatment. The clinical therapeutic effects were compared between the two groups. Results There were statistically significant post-treatment differences in the symptom severity score and the symptom frequency score between the treatment and control groups (P＜0.05). The total efficacy rate was 95.9% in the treatment group and 83.7% in the control group; there was a statistically significant difference between the two groups (P＜0.05). Conclusion Combined use of acupuncture and medicine is an effective way to treat functional gastric acid regurgitation.

Objective To assess the velocity patterns of right ventricular long axis myocardial motion during isovolumic contraction phase in the patients with dilated cardiomyopathy(DCM) by Doppler tissue imaging(DTI).Methods Right ventricular systolic free wall myocardial(the place close to the tricuspid annular) motion velocities were examined by DTI on apical four-chamber view in 28 patients with DCM and 30 healthy volunteers.Peak positive and negative velocities(V_(IVC1),V_(IVC2)),velocity time integrals(VTI_(IVC1),VTI_(IVC2)),times(T_(IVC1),T_(IVC2)) of right ventricular free wall myocardial motion during isovolumic contraction,and the peak velocity(V_S),time(T_S),velocity time integral(VTI_S) of ejection wave(S) were measured.Right ventricular percent changes in area(RVPCA) on three different views were calculated.Results V_(IVC1),V_S,VTI_S and differential IVC(the difference between V_(IVC1) and V_(IVC2)) in the patients with DCM were significantly decreased than those in control group(P(0.05)).Conclusions The changes of DTI indices of right ventricular long axis myocardial motion during isovolumic contraction in the patients with DCM are characteristic,which could be a new method to evaluate right ventricular systolic function.