1.EFFECTS OF BERBAMINE HCL ON ISOLATED WORKING HEART OF GUINEA PIG
Baofeng YANG ; Baiyan LI ; Xichen WU ; Wenhan LI
Chinese Pharmacological Bulletin 1987;0(01):-
The isolated working heart of guinea pig perfused with Tyrode's solution could work normally for at least 60 min. It was shown that berbamine ( BA ) could depress the function of isolated working heart of guinea pig in dose-dependent manner. BA 3 mol/L could decrease the left ventricular pressure, aortic pressure, -dP/dtmax, aortic blood flow and coronary blood flow, and increase left ventricular end-diastolic pressure. BA 100 mol/L could result in the ventricular asystole, however, no obvious influence the contraction of atrium.It was also demonstrated that BA could antagonise the arrhythmias induced by the adrenaline in isolated working heart of guinea Pig.
2.miR-10a inhibits cell proliferation and promotes cell apoptosis by targeting BCL6 in diffuse large B-cell lymphoma.
Qian FAN ; Xiangrui MENG ; Hongwei LIANG ; Huilai ZHANG ; Xianming LIU ; Lanfang LI ; Wei LI ; Wu SUN ; Haiyang ZHANG ; Ke ZEN ; Chen-Yu ZHANG ; Zhen ZHOU ; Xi CHEN ; Yi BA
Protein & Cell 2016;7(12):899-912
The BCL6 (B-Cell Lymphoma 6) gene is a proto-oncogene that is often expressed in diffuse large B-cell lymphomas (DLBCLs). BCL6 loss of function can kill DLBCL cells, demonstrating that BCL6 is necessary for the survival of DLBCL cells and could be a therapeutic target. In this study, we found that BCL6 protein levels were consistently upregulated in DLBCL tissues, whereas its mRNA levels varied randomly in tissues, suggesting that a post-transcriptional mechanism was involved in BCL6 regulation. We used bioinformatics analysis to search for miRNAs, which potentially target BCL6, and identified specific targeting sites for miR-10a in the 3'-untranslated region (3'-UTR) of BCL6. We further identified an inverse correlation between miR-10a levels and BCL6 protein levels, but not mRNA levels, in DLBCL tumor tissue samples. By overexpressing or knocking down miR-10a in DLBCL cells, we experimentally validated that miR-10a directly recognizes the 3'-UTR of the BCL6 transcript and regulated BCL6 expression. Furthermore, we demonstrated that negatively regulating BCL6 by miR-10a suppressed the proliferation and promoted apoptosis of DLBCL cells.
3' Untranslated Regions
;
Apoptosis
;
Cell Line, Tumor
;
Cell Proliferation
;
Gene Expression Regulation, Neoplastic
;
Gene Knockdown Techniques
;
Humans
;
Lymphoma, Large B-Cell, Diffuse
;
genetics
;
metabolism
;
therapy
;
MicroRNAs
;
genetics
;
metabolism
;
Proto-Oncogene Proteins c-bcl-6
;
biosynthesis
;
genetics