Cells of BT_(325), a human glioblastoma cell line, were treated with 0.5% Triton X-100 and immunostained with a monoclonal antibody to vimentin and antiserum to glial fibrillary acidic protein (GFAP), and followed by fluorescence labeled secondary antibody. After observation with fluorescence microscope the cells were dehydrated in increasing grades of ethanol and then critical point-dried. The carbon-platinum coating replicas of cytoskeleton were observed by transmission electron microscopy. The morphology of whole cytoskeleton as well as the arrangement of microtubules, microfilaments and intermediate filaments were clearly observed by this technique. It was observed that the diameter of intermediate filaments highly increased in the cells stained with the monoclonal antibody to vimentin. In the samples stained with anti-GFAP intermediate filaments were decorated in some cells but undecorated in other cells. The results indicates that all BT_(325) cells express vimentin, but only a fraction of the cells express GFAP. It was also observed that if the cells were fixed with formaldehyde before immunostaing with the monoclonal antibody to vimentin, the diameter of the intermediate filaments only slightly increased. However, the diameter of the intermediate filaments highly increaed in the cells stained firstly with the monoclonal antibody to vimentin and followed by formaldehyde fixation. This result is in accord with that of our previous immunofluorescence study. It indicates that formaldehyde fixation may "mask" certain epitopes on vimentin molecules.

Objective To analyze the influence of cochlear implant age, sexuality, and rehabilitation time on tone recognition ability.Methods 272 hearing-impaired children aged 2~6 years old with cochlear implants were enrolled in the study. By three factors mixed design,the sexuality, age and rehabilitation time of the children (independent variable) and the tone recognition rate (dependent variable) wereanalyzed. Results The rehabilitation time and age had significantly main effect in tone recognition rate (P<0.001), but age (P>0.05). The interactionof rehabilitation time and age also had main effect (P<0.001). Conclusion Age and rehabilitation time are important factors fortone recognition ability. The younger the children are, the better the recognition ability is with the extension of rehabilitation time.

Objective To explore the death distribution characteristics of heart disease and the strategies for its prevention and control, based on data of the death rates of different heart diseases among the residents of Henan province from 1987 to 2006. Methods The mortality rates of heart diseases during past 20 years were analyzed by age, sex, living places (urban vs. rural), and by patterns of heart disease as well with SAS9.1. Results In these past 20 years, the mortality rates of heart diseases ranged from 60.93 to 85.10 per 100 000. The annual mortality rate averaged 74.47 per 100 000, and the standardized mortality rate was 61.10 per 100 000, which equaled to 13.14% of the total death number. The death rates of heart diseases had increased significantly with age. Among the male and female of the cities or the rural, the death was mainly due to coronary heart disease. Concludes According to the death distribution characteristics of heart diseases in Henan Province, the prevention and control efforts should focus on the population over 35 years of age.

Objective To estimate the death number of children under age 5 in Linzhou city during 2004 to 2005 using the capture-recapture method, and to evaluate the integrality of data about the children death report in all death cause registry. Methods The data of children death were collected from sources of registry office, village symposium for the 3rd national death cause survey and police registries. Cases in every source were matched on the name, sex, age, date of death, cause of death and resident address. To fit for dependencies and heterogeneities between three sources, a log-linear model in stratified age and gender was used to estimate the death number of children which did not appear in three sources. Results It was reported that death number of children under age 5 was 359 and the mortality was 2.16% for combined three sources; but from the registry office, the number and mortality were 291 and 1.75%, respectively. The capture-recapture method estimated that the number of death was 398 and the mortality was 2.39%. Under-reporting rates of registry and aggregate database were 26.9% and 9.8%. Female deaths were more likely to go unreported. Under-reporting rates of registry got higher with the decrease of the age at death. Conclusion Registry office or aggregated database underestimated the death number of children under age 5. On the base of data which were collected from police, village and registry, using the capture-recapture method can adjust the death number of children.

Objective To revise the assessment of consonant vowel and tone recognition for cochlear implanted children. Methods 300 normal hearing children aged 2~6 years old were assessed with word lists for consonant and vowel recognition, and analysed with equivalency analysis. Results The incidence of consonant vowel and tone recognition was less than 90% in children aged 2~3 years old, but more than 90% in those aged 3 or more. Conclusion The assessment of consonant vowel and tone recognition for cochlear implanted children aged of 3 and above has been modified, and the assessment for those aged 2~3 years old has been developed.

Objective:To establish a method for the determination of oleanolic acid in She medicine Clematis florida var. plena. Methods:The HPLC method was carried on a Lanbo-Kromasil C18 chromatography column using acetonitrile-water ( 86∶ 14 ) as the mobile phase. The detection wavelength was 205 nm, the flow rate was 1. 0 ml·min-1 , and the column temperature was 25 ℃. Re-sults:The calibration curve of oleanolic acid was linear within the range of 0. 52-15. 60μg (r=0. 999 9). The average recovery of oleanolic acid was 92. 13%(RSD=3. 0%, n=9). Conclusion:The method is simple, rapid, accurate and reproducible, which can be used as an ideal method for the quality control of She medicine Clematis florida var. plena. .

60 years, 13.3% of the total sarcoidosis patients) diagnosed as sarcoidosis based on national criteria from the year 1965 through 1999 were reviewed. Results The clinical degree was as follows: phase I, 8 cases; phase Ⅱ, 6 cases; phase Ⅲ, 2 cases. The elderly sarcoidosis patients manifested variously, with misdiagnosis rate being 50%. The active degree of pulmonary sarcoidosis was correctly evaluated after the diagnosis was confirmed and the patients was strictly observed during the treatment since it was very important for avoiding the occurrence of complications. Conclusions The early diagnosis in elderly patients with sarcoidosis should be emphasized to prevent them from missing and misdiagnosis. The elderly patients with treating indications in phases Ⅱ and Ⅲ could be given steroids and constant observation and regular examinations are important during the treatment.

OBJECTIVE:To compare therapeutic efficacy and safety of laparoscopy combined with dienogest and laparoscopy combined with dienogest and TCM in the treatment of endometriosis. METHODS:126 endometriosis patients were selected and divid-ed into control group and observation group,with 63 cases in each group. Control group received combined with dienogest(25 mg/time,qd);observation group was additionally given TCM on the basis of control group. Therapeutic efficacy,recovery time of ovula-tion,menstrual recovery time and ADR were compared between 2 groups,and recurrence and pregnancy of them were followed up for 2 years. RESULTS:The total effective rate of observation group (93.65%) was significantly higher than that of control group (80.96%),with statistical significance (P<0.05). The menstrual recovery time and recovery time of ovulation in observation group were significantly shorter than control group,with statistical significance (P<0.05). The incidence of ADR in observation group (9.52%)was significantly lower than control group(26.98%),with statistical significance(P<0.05);the recurrence rate of observa-tion group (4.76%) was significantly lower than that of control group (17.46%),with statistical significance (P<0.05). In 2-year follow-up,there were 23 pregnancy patients in observation group,which was significantly higher than that of control group (9 cas-es),with statistical significance (P<0.05). CONCLUSIONS:Laparoscopy combined with dienogest and TCM in the treatment of endometriosis have better clinical effect,higher safety and lower recurrence rate. The patients are easily pregnant.

In surgical operation of the non-small cell lung cancer (NSCLC),the resection of lymph node involvement is highly correlated with the prognosis of NSCLC patients.The precise estimation of localized lymph node involved and the determination of relevant pattern of lymphadenectomy are the significant predictors for the prognosis and postoperative treatment in NSCLC patients.Therefore to identify the lymph node metastasis earlier has more important significance for tumor clinical staging and making therapeutic schedule.In recent years,the value of sentinel lymph node (SIN) has attracted more and more researchers attention in detecting the occult lymph node micrometastases and surgical staging.