Objective To investigate the clinical value of dual‐source CT (DSCT ) in assessing adult congenital heart disease (ACHD) .Methods The data in 29 ACHD patients with DSCT were retrospectively analyzed .Results Among 29 cases ,the major‐ity suffered from two or more cardiovascular malformations ,in which 20 cases had intracardiac malformations ,including 4 cases (13 .79% ) tetralogy of Fallot ,6 cases (20 .69% ) were atrial septal defect ,4 cases(13 .79% ) were bicuspid aortic valve (13 .79% ) and 8 cases (27 .58% )were ventricular septal defect ;19 cases had extracardiac malformations ,in which 3 cases(10 .34% ) were co‐arctation of the aorta ,4 cases (13 .79% ) were patent ductus arteriosus ,3 cases (10 .34% ) were transposition of the great arteries and 5 cases (17 .24% ) were anomalous pulmonary venous connection .Conclusion DSCT could not only clearly demonstrate the cardiovascular malformations in ACHD patients ,but also conduct the observation on the lung ,trachea and bronchia ,which can pro‐vide detailed anatomic information for surgery .

Objective To assess the value of pulmonary artery CT obstruction index for the evaluation of the severity of pulmonary embolism (PE),and to investigate the relation between pulmonary artery CT obstruction index and D-dimer levels.Methods 125 patients were diagnosed as PE by computed tomographic pulmonary angiography (CTPA)and D-dimer.Patients were separated into high-risk group and non-high risk group.CT obstruction index,D-dimer levels,diameter of the pulmonary artery were compared between two groups. Spearman’s rank correlation coefficients were used to assess the correlation between the CT obstruction index and the D-dimer levels,diameter of the pulmonary artery.Results CT congestion index of high-risk PE group was obviously higher than that of the non-high risk group (P=0.000).The diameter of pulmonary artery in high-risk PE group was obviously greater than that of the non-high risk group,the difference was statistically significant (P=0.000).No statistically significant difference was found in D-dimer levels between the two groups (P=0.103).There was no correction with CT congestion index and D-dimer levels(P=0.71).Conclusion The D-dimer levels of serum was a predictor of pulmonary embolism,cannot evaluate the severity of PE.CT obstruction index can reflect the severity of PE in some extent as an indicator of PE,there was no correlation with CT obstruction index and D-dimer levels.

In order to obtain the long-acting FSH preparation, the single strand long-acting analogous gene FSHbeta-CTP-alpha was successfully constructed by the C-terminal peptide(CTP) of carboxyl-terminal region of human chorionic gonadotropin with the goat FSHalpha-subunit and beta-subunit genes, then it was inserted into pPIC9K vector. The recombinant plasmid pPIC9K FSHbeta-CTP-alpha was transformed into Pichia pastoris GS115 by electroporation. The multi-copy inserts His+Mut+ were gained by the screening of phenotype and hyper-resistance to G418. After methanol induction, the supernatant was analysised by SDS-Polyacrylamide Gen Electrophoresis and Western blot. The results show that the transformants of FSHbeta-CTP-alpha could express the objective protein successfully and the molecular weight is about 29 kD. The concentration of supernatant was detected by Radio-immunoassay and the average expression of multi-inserts is 91.849 mIU/mL and the low-inserts is 37.419 mIU/mL. The expression of multi-inserts is higher than the low-inserts significantly. This research lay the foundation for studying the structure of FSH and the production of long-acting FSH preparation.
Animals ; Delayed-Action Preparations ; chemical synthesis ; Electroporation ; Female ; Follicle Stimulating Hormone ; analogs & derivatives ; genetics ; Genetic Vectors ; Goats ; Pichia ; genetics ; metabolism ; Recombinant Proteins ; administration & dosage ; biosynthesis ; genetics