1.Relationship between the expression levels of cell cycle regulators and the proliferation ability of human giant-cell lung carcinoma cell strains 95C and 95D
Hao WANG ; Haijiao CHEN ; Xiaying ZHANG
China Oncology 1998;0(04):-
Purpose:To study the molecular mechanism that d etermines the cell cycle differences between human giant-cell carcinoma cell st rains 95C and 95D. Methods:FACscan was used to analyze the cell cycle of 95C and 9 5D cells, and the proliferation ability of 95C and 95D cells was measured by MTT assay. We further detected the expression levels of cell cycle factors by Weste rn blotting. Results:Our data showed that the proliferation ability of 95D c ells is higher than that of 95C cells, and that the cell number in S phase of 95 D cells is much larger than that of 95C cells. We further found that the express ion level of p27 in 95D cell is lower than that of 95C cells. In addition, the e xpression levels of CDK2 and p-RB of 95D cells are higher than that of 95C cell s. Conclusions:Our results indicated that low expression level of p27 lead to the increased proliferation ability of 95D cells, which might reflec t the progression of human lung cancer cells in cell cycle facets.
2.Diagnosis of hyperchloremic triple acid-base disorders in burn patients and its mechanisms
Yexiang SUN ; Zaifu ZHANG ; Shousheng LI ; Changrong WANG ; Xiaying CHEN ; Yongjie WANG
Chinese Journal of Pathophysiology 2000;0(08):-
AIM:To study the diagnosis of hyperchloremic triple acid-base disorders (hyperchloremic TABD) and its mechanisms and to find out the cause of hyperchloremic TABD of the burn patients. METHODS:① 154 concomitant arterial blood gas and electrolyte panel of 113 burn patients were diagnosed by clinical approach. ② The plasma aldosterone concentrations of burn patients were tested by radioimmunoassay. RESULTS:9 cases of the 113 burn patients were hyperchloremic TABD. Their plasma aldosterone's values were significantly higher than that of the natural ( P
3.Clinical Observation of No Difference in Incision Inflammation in Preoperative Skin Preparation at Different Time
Xiaying ZHANG ; Xiurong CHEN ; Xueqin LUO ; Qingyuan LIU ; Xiaoyan SUN ; Qing YE
Chinese Journal of Practical Nursing 2001;17(6):41,56-
The fact that incision inflammation ratio is related to the time of preoperative skin preparation was explored.227 cases made skin preparation a day or two hours before surgical operation were analysed prospectively in this study.There is statistical significant difference between the two periods of preoperative skin preparation:the second (two hours before surgical operation) can greatly lower the ratio of incision inflammation.
4.Explanation and Elaboration for the ARRIVE Guidelines 2.0—Reporting Animal Research and In Vivo Experiments (Ⅳ)
Xiaying LI ; Yonglu TIAN ; Xiaoyu LIU ; Xuancheng LU ; Guoyuan CHEN ; Xiao LU ; Yu BAI ; Jing GAO ; Yao LI ; Yufeng TAO ; Wanyong PANG ; Yusheng WEI
Laboratory Animal and Comparative Medicine 2023;43(6):659-668
Improving the reproducibility of biomedical research results is a major challenge.Transparent and accurate reporting of the research process enables readers to evaluate the reliability of the research results and further explore the experiment by repeating it or building upon its findings. The ARRIVE 2.0 guidelines, released in 2019 by the UK National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs), provide a checklist applicable to any in vivo animal research report. These guidelines aim to improve the standardization of experimental design, implementation, and reporting, as well as the reliability, repeatability, and clinical translatability of animal experimental results. The use of ARRIVE 2.0 guidelines not only enriches the details of animal experimental research reports, ensuring that information on animal experimental results is fully evaluated and utilized, but also enables readers to understand the content expressed by the author accurately and clearly, promoting the transparency and integrity of the fundamental research review process. At present, the ARRIVE 2.0 guidelines have been widely adopted by international biomedical journals. This article is a Chinese translation based on the best practices of international journals following the ARRIVE 2.0 guidelines in international journals, specifically for the complete interpretation of the ARRIVE 2.0 guidelines published in the PLoS Biology journal in 2020 (original text can be found at
5.Interpretation and Elaboration for the ARRIVE Guidelines 2.0—Animal Research: Reporting In Vivo Experiments (V)
Zhengwen MA ; Xiaying LI ; Xiaoyu LIU ; Yao LI ; Jian WANG ; Jin LU ; Guoyuan CHEN ; Xiao LU ; Yu BAI ; Xuancheng LU ; Yonggang LIU ; Yufeng TAO ; Wanyong PANG
Laboratory Animal and Comparative Medicine 2024;44(1):105-114
Improving the reproducibility of biomedical research results is a major challenge. Transparent and accurate reporting of the research process enables readers to evaluate the reliability of the research results and further explore the experiment by repeating it or building upon its findings. The ARRIVE 2.0 guidelines, released in 2019 by the UK National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs), provide a checklist that is applicable to any in vivo animal research report. These guidelines aim to improve the standardization of experimental design, implementation, and reporting, as well as enhance the reliability, repeatability, and clinical translation of animal experimental results. The use of the ARRIVE 2.0 guidelines not only enriches the details of animal experimental research reports, ensuring that information on animal experimental results is fully evaluated and utilized, but also enables readers to understand the content expressed by the author accurately and clearly, promoting the transparency and completeness of the fundamental research review process. At present, the ARRIVE 2.0 guidelines have been widely adopted by international biomedical journals. This article is based on the best practices following the ARRIVE 2.0 guidelines in international journals, and it interprets, explains, and elaborates in Chinese the fifth part of the comprehensive version of the ARRIVE 2.0 guidelines published in PLoS Biology in 2020 (the original text can be found at
6.Baicalin induces ferroptosis in bladder cancer cells by downregulating FTH1.
Na KONG ; Xiaying CHEN ; Jiao FENG ; Ting DUAN ; Shuiping LIU ; Xueni SUN ; Peng CHEN ; Ting PAN ; Lili YAN ; Ting JIN ; Yu XIANG ; Quan GAO ; Chengyong WEN ; Weirui MA ; Wencheng LIU ; Mingming ZHANG ; Zuyi YANG ; Wengang WANG ; Ruonan ZHANG ; Bi CHEN ; Tian XIE ; Xinbing SUI ; Wei TAO
Acta Pharmaceutica Sinica B 2021;11(12):4045-4054
Ferroptosis is a non-apoptotic regulated cell death caused by iron accumulation and subsequent lipid peroxidation. Currently, the therapeutic role of ferroptosis on cancer is gaining increasing interest. Baicalin an active component in