Objective:To study the influence of occlusal force change on apoptosis of periodontal ligament fibroblast(PDLF) and expression of proliferating nuclear antigen(PCNA) in the fibroblasts,so as to explore the possible mechanism for remodeling of periodontal ligament.Methods:Animal model of occlusal force change was established by extracting the right first,second and third maxillary molars in male SD rats.The rats were sacrificed at 6,12 hours and day 1,2,3,5,7,14,and 28 d after extracting the teeth(n=6),and their alveolar bone tissues were harvested.H-E staining was used to observe the morphologic changes of the alveolar bone tissues.TUNEL method and immunohistochemistry were used to detect apoptosis and PCNA expression in PDLF.The rats with normal occlusal forces were used as control(n=6).The results were assigned a mean score based on the percentage and the intensity of cells positively stained for TUNEL and PCNA.Results:Animal model of occlusal force change was successfully established.H-E staining showed that the control group had more pycnotic periodontal ligament,orderly fibres,and flatter alveolar bone than the model group.TUNEL result showed that the cell apoptosis reached its peak at 12 h after extracting the teeth(252.67?6.62),significantly higher than that of control group(7.17?2.32,P

Objective To evaluate the effectiveness and safety of TCM treatment to children with mycoplasma pneumonia. Methods RCTs of trational Chinese medicine in treating children with mycoplasma pneumonia were retrieved. CNKI, Wanfang Database and CBMdisc were searched for Chinese literature, and PubMed, EMbase and Cochrane Library were searched for foreign literature. All searching time was up to May 25, 2012. Data were picked up by two system reviewers individually and evaluation standard of Cochrane was used to evaluate the quality of selected literature. Results A total of 64 RCTs including 6289 cases of children with mycoplasma pneumonia were analyzed. In the improvement of clinical symptoms, among comparison between traditional Chinese medicine and western medicine (RR=1.10, 95%CI:-1.0--1.18) and comparison between traditional Chinese medicine plus western medicine and western medicine (RR=1.14, 95%CI:1.10-1.18), curative effects of treatment group were superior to the control group, P<0.00;For the fever clearance time, among comparison between traditional Chinese medicine and western medicine (RR=-4.26, 95%CI:-8.15--0.37), comparison between traditional Chinese medicine plus western medicine and western medicine (RR=-0.97, 95%CI:-1.27--0.66), curative effects of treatment group were superior to the western medicine group. For the time of the disappearance of cough and pulmonary rales, and the basic X-ray absorption time, curative effects of treatment group were also superior to the control group. Untoward effects in western medicine group were obvious than that of traditional Chinese medicine group. Conclusion TCM could improve and shorten the duration of symptoms of children with mycoplasma pneumonia. However, it is still necessary to be verified with more multi-center, double-blind, large sample randomized controlled trials, since the literature which have been brought into related researches were with low quality.

Objective To analyze the correlation between Uyghur metabolic syndrome and northwest dryness syndrome in Kashgar prefecture, Xinjiang. Methods Case-control design was used to include a total of 120 cases from the First People’s Hospital of Kashgar Prefecture, Xinjiang. They were selected as the case group, and 120 cases without diseases of Western medicine were as the control group. The northwest dryness syndrome was statistically analyzed. Results The total integral of northwest dryness syndrome in case group was (11.49±0.89) and that in control group was (5.84±0.81), and the case group was higher than the control group with statistical significance (P<0.01). By comparing various accompanied symptoms, except for the fire & wind dryness syndrome of lung&heart&spleen as well as lung&stomach yin deficiency syndrome, all the other syndromes in case group were higher than those in control group (P<0.01). The attack rate of northwest dryness syndrome in case group was 24.17% (29/120), higher than that in control group (7.50%, 9/120), with significant difference (P<0.01). In the correlation analysis between the state of TCM syndrome and northwest dryness syndrome in case group, spleen yin deficiency syndrome of metabolic syndrome had the strongest correlation with damp accumulation syndrome of northwest dryness syndrome (P<0.01), followed by liver-qi stasis syndrome and heart&kidney yin deficiency syndrome (P<0.01). Conclusion Metabolic syndrome is often concomitant with northwest dryness syndrome in Kashgar prefecture, Xinjiang. Moreover, the severity of clinical symptoms of metabolic syndrome was associated with the state of northwest dryness syndrome, which provides clinical epidemiology basis for developing prevention and cure strategy of metabolic syndrome in Xinjiang from the perspective of“treating the different diseases with the same therapy”.

Objective To study the application effect of different internal fixation scheme in older children with proximal hu-merus fractures .Methods The clinical data of 102 cases of elder children with proximal humerus fracture from March 2009 to Feb-ruary 2014 in the hospital were retrospectively analyzed according to different internal fixation methods ,they were divided randomly into A ,B ,C group ,34 cases in group A ,using elastic intramedullary retrograde intramedullary internal operation ;32 cases in group B ,using open reduction and Kirschner wire internal fixation operation ;27 cases in group C ,using open reduction and plate internal fixation operation .Compared and study the operation treatment effect on children in three groups .Results The operation time ,hos-pitalization time ,fracture healing time ,the excellent and good rate of postoperative shoulder joint Neer score ,complication rate of patients among the three groups had no statistical significance (P > 0 .05) .Compared with group B and group C ,the amount of bleeding was significantly decreased and incision length was significantly shortened in group A(P< 0 .05) .Conclusion Elastic intr-amedullary retrograte internal fixation ,kirschner wire interal fixation ,open reduction internal fixation ,all can fix proximal humeral fractures in older children safely and effectively ,and the clinical effect of elastic intramedullary retrograte internal fixation is better than other two fixation .

Objective To compare the prevent effect of biliary retrograde infection in the uncut and cut Roux-en-Y cholecystojejunostomy. Methods Sixteen Begle dogs were divided into two groups randomly. One group was performed with the uncut Roux-en-Y cholecystojejunostomy(the uncut group) and the other was performed with the cut Roux-en-Y cholecystojejunostomy (the cut group). The categories of bacterium in the the Roux limb before and twelve weeks after operation, and the biliary duct pressure of the anastomotic stoma were detected. The changes of the categories of bacterium before and after operation, the categories of bacterium and the biliary duct pressure after operation in two groups were analyzed. Results The isolating rate of G-aerobic bacteria and anaerobic bacteria was 37.50% and 6.25% before operation; in the uncut group, the isolating rate was 62.50%, 12.50%,with no significance difference comparing to preoperation(p＝0.390、1.000). In the uncut group the isolating rate was 87.50%, 75.00%after operation, with significance difference comparing to preoperation (p＝0.033、0.001).The isolating rate of anaerobic bacteria after operation in the cut group is higher than the uncut group,the difference is significance (p＝0.041). And the biliary duct pressure in the cut group is higher than the uncut group(p<0.001).Conclusion The uncut Roux-en-Y cholecystojejunostomy was more effective in prevent biliary retrograde infection.

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Objective To investigate the value of endoscopic submucosal dissection (ESD) and submucosal tunneling endoscopic resection (STER) for the diagnosis and therapy of gastric ectopic pancreas.Methods A total of 86 patients who were suspicion diagnose with gastric ectopic pancreas received ESD or STER in hospital,and the therapeutic effect and safety were followed-up.Results Fifty-four gastric ectopic pancreas patients were definitely diagnosed by postoperative pathology.Of the 54 patients,43 cases were located at the gastric antrum,7 cases were located at gastric fundus and gastric corpus juncture,4 cases were located at gastric corpus.Forty-five cases received ESD,9 cases received STER,rate of completely resection was 88.9％ (48/54),6 cases had a little tissue residual after resection.One case (1.9％,1/54) happened postoperative delay-bleeding,intraoperative and postoperative perforation was not found.During 1-32 months followed up,recurrence was not found.Conclusion ESD could excise the whole lesion to offer an accurate pathology diagnose,meanwhile good for treatment,ESD is an effective and relatively safe method for gastric ectopic pancreas,STER may be a new approach for gastric ectopic pancreas.

ObjectiveTo study the change of autophagy of human pancreatic cancer cell MiaPaCa-2 before and after Ad5-KAI1 tranfection,and to investigate the possible mechanism.MethodsThe MiaPaCa-2 cells without KAI1 expression were infected with Ad5-KAI1 with KAI1 target gene,and Ad5-null was used as negative control,and parental cell was used as blank control.The formation of autophagosomes was observed by electromicroscopy.The green fluorescent protein-labeled light chain 3 (LC3) associations with autophagosome membranes was detected by confocal microscopy.PD98059,LY294002 were applied to pre-treat the cells.The expression levels of beclin 1,AKT,ERK,the phosphorylation of AKT and ERK protein and the ratio of LC3-Ⅱ to LC3- Ⅰ were detected by Western blotting.ResultsAfter 100 MOI Ad5-KAI1 infections for 24 h,the rate of cell expressing KAI1 protein reached (84.97 ±8.56)％,number of LC3 increased from 4 to 20; and swelling,degeneration of mitochondria was observed,and bilayer-like structure in cytoplasm was found.The expression of beclinl increased (1.4 ±0.3 ) folds,and the expression of LC3-Ⅱ/LC3- Ⅰ increased (8.00 ±2.78) folds.PI3K blockade LY294002 pretreatment significantly suppressed the phosphorylation of AKT of MiaPaCa-2 (2.756 vs 1.516),but it did not inhibit the increase of ratio of LC3-Ⅱ to LC3- Ⅰ(0.770 vs 1.403).ERK blockade PD98059 pretreatment not only significantly suppressed the phosphorylation of ERK of MiaPaCa-2 ( 1.637 vs 0.403 ),but also inhibit the up-regulation of beclin 1 protein expression ( 2.377 vs 1.150) and increase of ratio of LC3- Ⅱ to LC3- Ⅰ (2.225 vs 0.680).ConclusionsKAI1 can significantly induce autophagy of human pancreatic cell line MiaPaCa-2 through phosphorylation of ERK rather than AKT.

Objective To explore the pathway of KAI1 induced autophagy regulating apoptosis in human pancreatic cancer cell line MiaPaCa-2.Methods There were three groups in the experiment,which were extracellular regulated protein kinases (ERK) phosphorylation inhibitor PD98059 pretreated group,Caspase-3 activation inhibitor VAD-FMK pretreated group and no PD98059 or VAD-FMK pretreated groups.And each group was divided into three sub groups with different treatment,which were adenovirus AD5-null vector infected control group,the human KAI1 gene recombinant adenovirus vector AD5 KAI1 infected group and autophagy inhibitor 3-MA pretreated and AD5-KAI1 infected group.The cell apoptosis was observed by AnnexinV-FITC/PI double staining.Caspase-3 activation level was evaluated by flow cytometry.ERK phosphorylation and poly(ADPribose) polymerase (PARP) cleavage were determined by Western blot.Results After the cancer cells infected with AD5 KAI1,KAI1 protein was expressed and GFP-LC3 green particles increased.Caspase-3 activation,PARP cleavage,ERK phosphorylation and apoptosis increased obviously.After autophagy inhibitor 3-MA pretreated,the percentage of apoptosis increased from (63.0 ± 7.9)％ to (88.0±4.5) ％ and Caspase-3 activation increased from (34.0±2.8) ％ to (44.2±4.0) ％ and PARP cleavage more.The apoptosis induced by 3-MA could be totally inhibited by Caspase-3 activation inhibitor VAD-FMK pretreated but could not be inhibited by ERK phosphorylation inhibitor PD98059.Conclusion KAI1- induced autophagy inhibits apoptosis through the downregulation of Caspase-3activation and PARP cleavage instead of ERK phosphorylation.

Effect of opsonized zpoosan on polymorphonuclear leukocyte (PMN)were studied by chemilundnescence (CL)and hydrogen peroxide (H2O2)assays. Results showed that different concentrations of Physalin B(PhB) presented a dose-dependent inhibitive effect on CL and H2O2 production of activated PMN. It suggested that the anti-inflammatory action of PhB may be related to the inhibition of production and release of oxygen free radical by PMN.