Objective: To quantitative analyze the expression of osteopontin(OPN) in oral squamous cell carcinoma(OSCC) tissues and its clinical significance.Methods: OPN expression was examined in 59 OSCC(with different clinical and pathological characteristics) and 12 normal oral mucosa(NOM) samples by using immunohistochemical method.The staining results were quantitatively studied by image analyzer.Results: OPN expression was much higher in OSCC sample than that in NOM sample(P

Objective To investigate the induction of specific anti-tumor immune response by transfected dendritic cells (DCs) with survivin mRNA of human pancreatic cancer, and to provide the experimental evidences for the treatment of human pancreatic cancer with DCs vaccine. Methods DCs were isolated and cultured from peripheral blood mononuclear cells (PBMCs). After being transcripted and amplified, survivin mRNA was transfected into DCs by electroporation. The expression of survivin in DCs at different time points was detected by quantitative real-time PCR. The survival rate of DCs before and after transfection was determined by MTT method. The induction of specific cytotoxic T lymphocyte (CTL) response by survivin mRNA transfected DCs was measured by 51Cr standard cytotoxicity test. The induction of specific CTL activation by survivin mRNA transfected DCs was evaluated through testing released IFN-γ by ELISA method. Results After survivin mRNA transfection for 48h, the expression of survivin mRNA in DCs reached the highest point (46.09±6.57). After transfection, the survival rate of DCs was stabilized around 80%. The DCs transfected with survivin mRNA could effectively induce HLA-A2+ / survivin+ specific CTL immune responses. Stimulated with pancreatic cancer cell line Capan-2 cells or SCL-1 cells as control group, the IFN-γ released in 24 hours by survivin specific CTL were (28.79±5.70) U/ml and (25.12±2.13) U/ml respectively, there was no significant difference (P=0.761). Conclusion The induction of CTLs by DCs transfected with human pancreatic cancer survivin mRNA could produce specific anti-tumor immunity.

Objective To investigate the best method of transfecting total RNA extracted from pancreatic cancer MiaPaCa-2 cells into dendrtic cells (DCs). Methods DCs were cultured from peripheral blood mononuclear cells induced by rhGM-CSF, rhIL-4 and TNF-α. Morphology of DCs was observed. Flow cytometry was used to detect the mature DCs specific surface markers:CD40, HLA-DR, CD83, CD86. Mixed lymphocyte (MLR) was used to determine the ability of DCs to stimulate allogeneic T cell proliferation.Liposomal transfection, electroporation method and passive transfection was used to transfect MiaPaCa-2 cell total RNA into DCs, Real time RT-PCR and MTT assay was used to determine the expression of MUC1 mRNA and the survival rate of the RNA transfected DCs. Results The cells acquired showed typical DCs morphology, the positive rate of CD40, HLA DR, CD83 and CD86 were 34.3% ,50.2% ,89.2% and 73.6%,and they showed a strong ability to stimulate allogeneic T cell proliferation. 48 h after transfection with MiaPaCa-2 cells total RNA by using electroporation, the MUC1 mRNA amount (45.39 ± 9.33) in DCs was higher than that of liposomes method (3 1. 68 ± 7.25) and passive transfection method (18.53 ± 3.26) . DCs survival rate was (80.36 ± 2.43)% by using electroporation, which was relatively lower than (91.48 ±5.42) % by using passive transfection method, but higher than (67.44 ± 2.51) % by using liposomes method,and it was stabilized around 80%. Conclusions Transfecting total RNA extracted from pancreatic cancer MiaPaCa-2 cells into DCs with electroporation is efficient and safe.

Objective:To study the influence of occlusal force change on apoptosis of periodontal ligament fibroblast(PDLF) and expression of proliferating nuclear antigen(PCNA) in the fibroblasts,so as to explore the possible mechanism for remodeling of periodontal ligament.Methods:Animal model of occlusal force change was established by extracting the right first,second and third maxillary molars in male SD rats.The rats were sacrificed at 6,12 hours and day 1,2,3,5,7,14,and 28 d after extracting the teeth(n=6),and their alveolar bone tissues were harvested.H-E staining was used to observe the morphologic changes of the alveolar bone tissues.TUNEL method and immunohistochemistry were used to detect apoptosis and PCNA expression in PDLF.The rats with normal occlusal forces were used as control(n=6).The results were assigned a mean score based on the percentage and the intensity of cells positively stained for TUNEL and PCNA.Results:Animal model of occlusal force change was successfully established.H-E staining showed that the control group had more pycnotic periodontal ligament,orderly fibres,and flatter alveolar bone than the model group.TUNEL result showed that the cell apoptosis reached its peak at 12 h after extracting the teeth(252.67?6.62),significantly higher than that of control group(7.17?2.32,P

Objective:To study the role of tetrodotoxin-resistant(TTX-R)human sodium channel of hNa v1.8 mRNA in trigeminal neuralgia (TN). Methods:The samples of affected trigeminal division(inferior alveolar nerve) and non-affected division(lingual nerve) were surgically obtained from six idiopathic TN patients to whom the drug therapy was not effective.The expression of hNa v1.8 mRNA was assessed by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) with ?-actin as an internal control. A sample of great auricular nerve (from a patient who had to accept the combined radical neck dissection with glossectomy and hemimandibulectomy) and a sample of infraorbital nerve (from a patient who had to accept subtotal resection of maxilla) were used as the negative controls. Resoults:The relative amount of hNa v1.8 mRNA in the affected trigeminal divisions(1.233?0.324) was higher than that in the non-affected divisions(1.018?0.249), t=3.919,P=0.011. Conclusion:The higher expression of hNa v1.8 mRNA in the affected nerves of TN patients may play an important role in TN.

Objective: To study the influence of occlusal force changes on apoptosis related gene Bcl-2 and Bax in rat periodontal ligament fibroblasts (PDLF). Furthermore, to explore the possible mechanism in remodeling of periodontal ligaments. Methods: Animal model of occlusal force changes was established by extracting the right first, second and third maxillary molars in male SD rats. Rats were sacrificed at 6,12 hours and 1, 2, 3, 5, 7, 14 and 28 days after teeth extraction(n=6), and their alveolar bone tissues were harvested. HE staining was used to observe the morphologic changes of alveolar bone tissues and immunohistochemistry were used to detect Bcl-2 and Bax expressions in PDLF. The rats of normal occlusal forces were used as control(n=6). The results were assigned a mean score based on the expressions and the intensity of cell positively stained for Bcl-2 and Bax. Results: Animal model of occlusal force changes was successfully established. HE staining showed that the control group had more pycnotic periodontal ligament, orderly aligned fibres, and flatter alveolar bone than model group. Immunohistochemical staining showed that expression of Bax reached its peak at 12 h after extracting the teeth(267.00?7.82), significantly higher than that of control group(25.67?4.97,P

砄bjective: To observe whether substance P(SP), calcitonin gene related peptide(CGRP), vasoactive intestinal polypeptide(VIP),? endorphin(? EP) are related to the pathogenesis of trigeminal neuralgia. Methods: During the attacks of trigeminal neuralgia, SP,CGRP,VIP,? EP in plasma from both external jugular and cubital fossa vein blood ipsilateral to the pain were assessed by sensitive radioimmunoassay in 16 ptients and 10 health controls, after operations the venous blood from the external jugular vein of the patients was sampled again; the external jugular vein blood of normal volunteers was collected as control. SP like and CGRP like immunoreactive positive granules of pain and painless branches were labelled using ABC immunohistochemistry methods; quantitative analysis was performed using HPIAS 1000 system. Results: During the attacks of trigeminal neuralgia, the levels of SP,CGRP,VIP in the external jugular vein blood were significantly higher than those in the cubital fossa vein blood, postoperative external jugular vein blood and the external jugular vein blood of normal volunteers. The plasma levels of ? EP in patients with trigeminal neuralgia were significantly lower than that in normal volunteers.The number and area of SP like ,CGRP like immunoreactive positive granules in the pain branches was much more and larger than those in the painless branches . Conclusion: Abnormal synthesis and release of neuropeptides in patients may be related to the pathogenesis of trigeminal neuralgia.

Objective: To summarize the experience of repairing or al and maxillofacial defects with radial forearm free flap. Methods: The clinical records of 110 cases received oral and maxillofacial reconstruction with radial forearm free flap were analyzed.Results: Forearm free flap healed well in 10 5 out of the 110 cases.with the overall success rate of 96.4%.Failure was in 4 c ases of cancer.One patient died from cerebral infarction 19 days after operation .Post operation infection did not result in the necrosis of the flaps because o f corresponding treatment in 5 cases.Conclusion: The radia l forearm free flap characterized by the anatomical consistency,easy fold, ideal thickness, flexibility and long pedicle, is feasible in the reconstruction of soft tissue defects in oral and maxillofacial region.

OBJECTIVE To explore the correlation of allergic rhinitis symptoms in preschool children with the household environment dust mites and house dust-mite-specific IgE, as to provide theoretical basis for pediatric allergic disease prevention and control work. METHODS The children with rhinitis who was born in the Second People's Hospital of Wuhu were selected in this study. When the children were 3 or 4 years old, they were followed up. The allergic rhinitis symptoms integral of the children were evaluated, the household environment dust samples of the children were collected. The content of dermatophagoides pteronyssinus allergen components 1(Der p1) and dermatophagoides farinae allergen components 1(Der f1) in dust samples were determined by the method of enzyme-linked immunosorbent(ELIEA). The children house dust-mite-specific serum IgE(sIgE) level was also examined. RESULTS Pediatric allergic rhinitis symptoms had a positive correlation with dermatophagoides pteronyssinus sIgE and dermatophagoides farinae sIgE(P<0.05); Pediatric allergic rhinitis symptoms had a positive correlation with Derp1 and Derf1(P<0.05). There were no statistically significant correlation between Der p1 and Der f1 exposure levels and dust mite sIgE level (P>0.05). CONCLUSION The allergic rhinitis symptoms of the children are positively correlated with household environment dust mites and dust mites specific IgE. That indicated the severity of allergic rhinitis symptom in children may be determined by detecting specific IgE levels. The prevention of allergic disease and improvement the quality of life of the allergic children can be done by strengthening the health education of dust mites and decreasing the content of bedroom environment dust mites.

The tongue and its surrounding structures have special anatomical and functional characters and how to restore its shape and function after glossectomy (especially total glossectomy) still remains a difficult problem. The authors devised a new method of repair by using a forearm free flap with results in 16 out of 17 patients. The selection and shaping of the flap, the evaluation of the recipient site and its blood vessels, the prevention of the oral infection,the monitoring of the circulatory status of the flap and the evalution of the operative result were discussed.