BACKGROUND: Studies have proved that rotating magnetic field(RMF) can significantly increase bone mineral density(BMD) in rats, and such high BMD maintains for a long time which is independent of the actions of hormone.OBJECTIVE: To investigative the effect of RMF on bone calcium content and the correlation between bone-specific alkaline phosphatase(BAP) and deoxypyridinoline crosslinks (DPD) in rats.DESIGN: A randomized controlled study in rats.SETTING: A college of life sciences of a university.MATERIALS: The experiment was conducted in Shenzhen Key Lab of Microbiological Genetic Engineering from March 2004 to October 2004, using 90 healthy adult SD rats including 60 females weighing(259 ± 70) g and 30 males weighing (351 ± 104)g. The rats were randomly divided into 9 groups including 6 ovariotomy groups, namely female sham-operation group, normal calcium control group, low calcium control group, low calcium experimental group, normal calcium experimental group, and low calcium group with traditional Chinese medicine(TCM) treatment, besides a male control group,male low calcium experimental group, and male normal calcium experimental group, with 10 rats in each group.INTERVENTIONS: The female rats in each group, except for those in the sham-operation group, received ovariotomy. The rats in the ovariotomy control group had only ovariotomy. After metabolic exhaustion of the remnant estrogen on the 15th day, the rats in the ovariotomy experimental group were treated with RMF for 2 hours once a day for 15 days. The male rats, except for those in the control group, all bad RMF in identical manner. The rats were given food with normal calcium(containing 0.26% calcium), lower calcium (containing 0.1% calcium) and traditional Chinese medicine(powder of Fructus Psoraleae, Radix Astragali, epimedium herb, and herba cistanchis) .After RME treatment, the rats were fed for another 15 days before the femur was collected to measure the bone calcium content. Serum samples were also obtained for measuring the content of BAP and urine samples collected to measure the content of DPD.MAIN OUTCOME MEASURES: The bone calcium content of each group and serum BAP and urine DPD.RESULTS: Of the 90 rats used, 4 died during the experiment and 86 entered the result analysis. The bone calcium content was increased after RMF treatment, and the content in rats in the low calcium group with ovariotomy and TCM treatment and lower calcium control group with ovariotomy was(0.226± 0. 015) and(0. 206 ± 0. 015) g/g, respectively( t =4.63, P ＜ 0. 05). The bone calcium content in the male normal calcium experimental group and male control group was(0. 206 ±0. 031) and(0. 199 ±0. 014) g/g respectively ( t = 4.21, P ＜ 0.05 ) . After treatment with RMF, serum BAP content was increased and was(20. 52 ± 1.78) and(15.68 ±3.68) U/L in normal calcium experimental and control groups with ovariotomy, respectively( t=4.76, P ＜0.05), and(17.69±3.78) and (8.53±2.54) U/Lin the male low calcium experimental and male control groups, respectively( t= 4. 59, P ＜ 0.05). Urine DPD after RMF treatment was (86.97 ± 37.19)and(401.57 ±79. 34) nmol/L in the low calcium experimental group with TCM treatment and ovariotomy and the low calcium control group with ovariotomy, respectively( t =7.45, P ＜ 0.01), and(97.87 ±31.97) and (168.71 ±53.19) nmol/L in the male normal calcium experimental and male control groups respectively( t = 8.31, P ＜ 0.01 ).CONCLUSION: RMF can effectively increase bone calcium content in a short time( 15 days), and the effect was positively correlated with increased blood BAP and decreased urine DPD.

Objective · To study the expression of microRNA-143 (miR-143) in human colon cancer and adjacent normal tissues, and to explore its effect on the function of colon cancer cells and downstream target genes. Methods · Lentiviral expression vector of miR-143 was designed and used to stably transfect colon cancer cell lines HCT116 and RKO. Real-time PCR was used to detect the expression of miR-143 in colon cancer, adjacent normal tissues, HCT116 cells and RKO cells infected with lentivirus. The apoptosis ratio of HCT116 cells and RKO cells was detected by Annexin V-APC single staining and flow cytometry. The migration ability of HCT116 cells and RKO cells was detected by Transwell method. The expression of FOSL2 protein was detected by Western blotting assay. Results · The expression of miR-143 in colon cancer tissue was significantly lower than that in adjacent mucosal tissue (0.339±0.454 vs 1.003±0.003) (U=16.000, Z=-4.231, P=0.000). By up-regulation of miR-143, apoptosis rates were significantly higher in HCT116 and RKO cells than those in the negative controls (P=0.000). The count of migrating HCT116 cells in up-regulated group was significantly lower than that in the negative control group (P=0.000). The count of migrating RKO cells in down-regulated group was significantly higher than that in the negative control (P=0.003). By down-regulation of miR-143, the expression of FOSL2 protein was increased. Conclusion · The expression of miR-143 was significantly decreased in colon cancer tissues. Up-regulation of miR-143 can promote the apoptosis rate in both cell lines, and inhibit the migration ability of HCT116 cells. Down-regulation of miR-143 could promote the migration ability of RKO cells. By inhibiting miR-143, the expression of FOSL2 protein in colonic cancer cells would be increased.

Multiple myeloma is a monoclonal plasma cell malignant proliferative disease,accounting for about 10％ of malignaut tumors of blood system.In recent years,due to the application of hematopoietic stem cell transplantation and the application of MDT by combining new drug such as thalidomide,lenalidomide and bortezomib,the average survival of MM patients was significantly longer than before.The treatment strategy should be individualized before treatment according to the patient's age,physical condition and complications.In this paper,the treatment strategy of multiple myeloma patients were reviewed.

Objective To investigate the clinical efficacy of atorvastatin in patients with lacunar infarctions (LI) and influence on cerebrovascular reactivity(CVR). Methods 60 patients with LI were randomly divided into observation group and control group. The patients of control group were given aspirin treatment, patients of observation group were given atorvastatin combination with aspirin therapy. The breath-holding index(BHI) before and after treatment was calculated. After continuous treatment for 3 months the clinical efficacy was evaluated. Results The total effective rate of the observation group was 90. 0% ,it was significantly higher than that of the control group(73.3% ),compared with the control group the difference was significant( x2 = 10. 428 ,P < 0. 05). The bilateral BHI of observation group significantly increased, compared with per-treatment and control group the difference was significant ( t =9. 455,6. 135 ,all P < 0. 05 ) ,but the bilateral BHI of control group had no significant difference compared with pertreatment ( P ＞ 0. 05 ). Conclusion Atorvastatin could effectively improve CVR in patients with LI. Therefore, atorvastatin had great significance for the prevention of stroke.

Objective\ The adrenal gland is a linkage between nervous system and endocrine system.Its cortex results from mesoderm,and medulla derives from neuroectoderm.Searching its double characteristic of nervous and endocrine tissue is our aim. Methods\ Histochemical and immunohistochemical staining techniques were used,and immunostained sections were observed under a light microscopy. Results\ NPY positive neuron and nerve fiber,CGRP positive nerve fiber existed in adrenal cortex.NADPH d positive neuron and nerve fiber, c fos positive neuron and nerve fiber,CGRP positive nerve fiber,SP positive nerve fiber were detected in adrenal medulla.NADPH d positive cell,NPY positive cell,CGRP positive cell,SP positive cell were found among the adrenal chromaffin cells.The zona glomerulosa,zone fasciculata and zona reticularis were stained strongly by NADPH d in cortex. Conclusion\ The adrenal gland was innervated by NO ergic,NPY ergic,CGRP ergic,SP ergic, c fos ergic nerves.The adrenal parenchyma,especially chromaffin cells can produce NO,NPY,CGRP,SP which regulate adrenal endocrine by itself.The nervous control of adrenal function and neuroendocrine function of adrenal gland is more complex than hitherto believed.\;

Objective Our purpose is to explore the occurrence and distribution of glutamate and metabotropic glutamate receptor in earthworm, P. aspergillum and to analyze their function significance. Methods By employing immunocytochemistry tchnique, the positive cells of glutamate and metabotropic glutamate receptor, mGluR1, mGluR2/3, mGluR4 in earthworm, P. aspergillum were investigated under a light microscope. Results It was found that glutamate positive cells existed in cerebroganglion, subpharyngeal ganglion, ventral ganglion, neurons between muscle cells,intestinal epithelium and epidermis, that thin and dense glutamate positive fibers distributed in pharyngeal nerve cycle, subpharyngeal ganglion, ventral neurochain, that mGluR1 or mGluR4 positive neurons were detected in cerebroganglion and mGluR2/3 positive neurons weren't observed in earthworm. Conclusion During glutamate positive neurons, some possess remarkable processes and matured appearance from which glutamate positive fibers in pharyngeal nerve cycle, subpharyngeal ganglion, ventral neurochain derive, another have few branches, unmatured appearance in which glutamate doesn't serve as neurotransmitter acting to mGluR1 or mGluR4 of neurons in earthworm cerebroganglion.

Objective:To study the influence of gonadal hormones on the function of lymphocytes of SLE patients through detecting the expression of estrogen receptor alpha(ER?) and the function of peripheral blood lymphocytes of SLE patients.Methods:Blood samples of 33 SLE patients and 31 normal controls were collected.Estradiol(E2) and testosterone (T) concentration in peripheral blood were detected by ELISA,the proliferating reaction of T lymphocyte stimulated by ConA were detected by methyl thiazolyl tetrazolium (MTT) assay and the expression of estrogen receptor alpha(ER?)mRNA of peripheral blood mononuclear cells (PBMCs),T lymphocytes and B lymphocytes were detected by RT-PCR.Results:Compared with normal control,the stimulating index of T lymphocyte proliferation decreased(P

Objective To explore the relationship between the ST-T continuous changes of ECG and the chronic coronary insufficiency.Methods 158 cases of ST-T continuous changes of ECG were treated with selective plate exercise tests and the coronary arteriography.Results 54 cases of plate exercise tests were positive(34.2%),104 cases were negative(65.8%).36 cases were diagnosed as the stenosis of coronary through the coronary arteriography(22.7%).But among 54 positive cases of plate exercise tests,33 cases had the stenosis of coronary,accounting for 91.6% in the cases of stenosis of coronary.Conclusion The quiescent ECG ST-T continuous changes aren't specfic changes of chronic myocardial ischemia.Most of them are caused by other reasons.

It mainly discusses the therapy of kidney edema on“Shanghan Lun”,which is caused by nephritis,nephrotic syndrome and renal failure.In addition to the manifestation and therapeutic principle of edema,the implication of some herbs combination is also described in detail.Meanwhile,some viewpionts are strengthened with concepts of modern medicine.In conclusion,Zhang Zhongjing puts importance on the etiology,pathogenesis of disease when treating edema.Therefore,ascending and discending Qi is highlightened as the general principle during the treatment of kidney edema.

Objective To establish two PCR assays to rapidly and simultaneously detect U.parvum and U.urealyticum.Methods Two PCR assays were established through designing two sets of specific primer targeting urease gene B of U.parvum and U.urealyticum,respectively.The standard strains of U.parvum and U.urealyticum and the clinical samples were detected by these PCR assays and PCR products of the standard strains and two clinical specimens were directly sequenced and carried out specific and sensitive assays.Forty-eight clinical specimens were tested for culture and the PCR assays and tow methods were compared.Results The standard strains of U.parvum,U.urealyticum and the clinical specimens were successfully and differentially detected by the PCR assays and the sequencing results were found to have a complete similarity to the GenBank sequences.The 14 strains of other bacterial genera including 5 strains that produced urease were not amplified by these PCR assays.Each assay had a detection limit of 10 copies/?l of plasmid DNA.The positive rate in 48 clinical specimens by PCR(54.2%)was higher than that by culture(39.6%)(P