35 cases of rotavirus enteritis occurring in months from September to December of 1980 and 1981 were reported. Typical rotavirus particles were found in 35 out of 54 stool specimens examined with electron microscope. The positive rate of EM was 64.8%.The age of the patients was ranged from 4 months to 2 years; 65.7% of them were 6 to 12 months old. There were 22 males and 13 females.The clinical manifestations consisted of diarrhoea, fever(80%), vomiting (62.8%), mild-to-moderate dehydration(85.7%), and metabolic acidosis(37.17%), upper respiratory infection symptoms(4g.57%). In 40% of the cases, the white cells in the feces were increased in number. Secondary bacterial infection occurred in 4 cases( 11%),which was higher than that of other reports.The main route of transmission was fecal-oral; the presence of respiratory symptoms in some patients suggested the possibility of air-borne spreading.The incidence of this disease was low in the new borns. It is most likely that they arc protected with the passive immunity obtained through the placenta. Furthermore, the structural and physiological characteristics of the gastro-intestinal tract of the neonatus are also contributive to reduce the disease in them.

During the health reforms development,medical service sets a higher demand for neo-type medical talents,thus it is of great significance to direct the ideals and beliefs education for medical students with scientific outlook on development tensely based on the condition of the ideological,study and living reality of medical students.

Objective To understand the differences of competency assessment of residents from the hospital director of clinical departments and resident physicians and to explore residency training mode for future ability training of the residents .Methods Study was performed in a third-grade class-A hospital in Beijing to understand the difference of capacity ,creativity evaluation be-tween the directors of clinical department and resident physicians through a questionnaire survey and statistical analysis .Results Results showed no statistically significant differences between the directors of clinical department and resident physicians in com-puter application ,and the remaining capacities were lower in the directors of clinical department than in the resident physicians .Be-sides ,the resident physicians hold that the residents were poor in research capacity ,creativity and legal awareness .Conclusion The study prompts us to strengthen the clinical expertise and skills training of residency ,also we should pay attention to the training of comprehensive ability .

Objective To observe the clinical efficacy of therapy of nourishing kidney and liver for the treatment of hypomenorrhea with the syndrome of kidney deficiency and liver stagnation. Methods Fifty-one hypomenorrhea patients with the syndrome of kidney deficiency and liver stagnation were randomized into treatment group(N =25) and control group (N = 26). The treatment group was treated with oral use of Yangyin Shugan Capsules (mainly composed of Radix Bupleuri, Radix Paeoniae Alba, Radix Rehmanniae Preparata, and Fructus Ligustri Lucidi). The control group was given placebo capsules orally. After treatment for 8 weeks , the overall efficiency and therapeutic effect on menstruation of the two groups were evaluated according the changes of traditional Chinese medicine(TCM) symptom scores. Results(1) The total effective rate of the treatment group was 84.0％, and that of the control group was 15.4％, the difference being significant between the two groups (P < 0.01). (2) The effective rate on the menstrual volume and menstrual color and texture of the treatment group was 64.0％, 68.0％, and that of the control group was 7.7％, 15.4％ respectively, the difference being significant(P < 0.01). However, the difference of effect on improving menstrual period was insignificant between the two groups (P > 0.05). Conclusion Therapy of nourishing kidney and liver is effective for the treatment of hypomenorrhea with the syndrome of kidney deficiency and liver stagnation, and has obvious effect on improving menstrual volume and TCM symptoms.

Objective Discuss the treatment mechanism of Xiaofeng Xuanqiao Decoction on guinea pig with al ergic rhinitis. [Methods]Sixty guinea pigs were randomized into 6 groups: normal control group, al ergic rhinitis(AR) group, high-dosage, equivalent-dosage, low-dosage Xiaofeng Xuanqiao decoction group, and loratadine group. AR guinea pig model was successful y established by ovalbumin(OVA) chal enge method. Xiaofeng Xuanqiao decoction and loratadine were applied to intervene AR guinea pig. The serum levels of IgE and IL-4, IFN-γ, histamine were determined by ELISA methods. [Results]The expression levels of IgE and IL-4, histamine significantly increased,meanwhile the level of IFN-γsignificantly lower compared with the normal group(P<0.01). Compared with that in AR model group, the serum level of IgE and IL-4, histamine in high-dosage Xiaofeng Xuanqiao decoction group significantly decreased,but the level of IFN-γ increased(P<0.01); the levels of IgE and IL-4, histamine decreased in other medication groups, meanwhile the level of IFN-γ increased compared with the model group(P<0.05).[Conclusion]Xiaofeng Xuanqiao can promote the produce of IFN-γ, inhibit the formation of IL-4, promote the secretion of Th1/Th2 cytokines to restore balance, to relieve eosinophil activation, reduce the synthet-ic of IgE, reduce the release of histamine, in many ways to prevent al ergic rhinitis inflammation, to achieve therapeutic effect.

Objective · To study the expression of microRNA-143 (miR-143) in human colon cancer and adjacent normal tissues, and to explore its effect on the function of colon cancer cells and downstream target genes. Methods · Lentiviral expression vector of miR-143 was designed and used to stably transfect colon cancer cell lines HCT116 and RKO. Real-time PCR was used to detect the expression of miR-143 in colon cancer, adjacent normal tissues, HCT116 cells and RKO cells infected with lentivirus. The apoptosis ratio of HCT116 cells and RKO cells was detected by Annexin V-APC single staining and flow cytometry. The migration ability of HCT116 cells and RKO cells was detected by Transwell method. The expression of FOSL2 protein was detected by Western blotting assay. Results · The expression of miR-143 in colon cancer tissue was significantly lower than that in adjacent mucosal tissue (0.339±0.454 vs 1.003±0.003) (U=16.000, Z=-4.231, P=0.000). By up-regulation of miR-143, apoptosis rates were significantly higher in HCT116 and RKO cells than those in the negative controls (P=0.000). The count of migrating HCT116 cells in up-regulated group was significantly lower than that in the negative control group (P=0.000). The count of migrating RKO cells in down-regulated group was significantly higher than that in the negative control (P=0.003). By down-regulation of miR-143, the expression of FOSL2 protein was increased. Conclusion · The expression of miR-143 was significantly decreased in colon cancer tissues. Up-regulation of miR-143 can promote the apoptosis rate in both cell lines, and inhibit the migration ability of HCT116 cells. Down-regulation of miR-143 could promote the migration ability of RKO cells. By inhibiting miR-143, the expression of FOSL2 protein in colonic cancer cells would be increased.

On the basis of the bioluminescent detection technology of the lux gene being a kind of new and progressing environmental detection technology,in this paper,the constitution,function,expression and regulation of the lux gene were introduced,moreover,the recent advance of its application in the study of environmental detection was reviewed

Objective To investigate the characteristics of cognitive changes in type 2 diabetes mellitus pa-tients complicated with stroke. Methods One hundred and one stroke patients were recruited and allocated to thetype 2 diabetes mellitus group (34 patients) and sixty-seven patients in the control group (67 patients) according tobeing with or without type 2 diabetes mellitus. All the patients were evaluated, after 3 weeks, 6 months and 12months of onset of stroke, with regard to their cognitive functions by using the Mini-mental State Examination(MMSE) and Montreal Cognitive Assessment (MOCA). Results Average scores obtained in MMSE and MOCAtests were significantly lower at 3 weeks and 6 months after onset in the type 2 diabetes mellitus group when comparingwith control group. In the type 2 diabetes mellitus group, no significant difference was observed between 3 weeks and6 months after onset of stroke with regard to scores of MMSE and MOCA ( P > 0.05 ), however, the score at 6 monthswas significantly lower than that at 12 months after onset (P <0.05). On the other hand, scores of MMSE (P >0.05 ) and MOCA (P > 0.05 ) in the control group were not any statistically different among various time points ofobservation. Conclusion Type 2 diabetes mellitus will aggravate cognitive dysfunctions of stroke patients, whichstarted to recover in 6 months of onset of the stroke.

Objective A retrospective study was conducted to evaluate the clinical significance of Video EEG monitoring in the diagnosis of epilepsy and other paroxysmal events Methods Video EEG monitoring under the state of awaking and sleeping and evoked tests were performed continuously in 216 patients with epilepsy and other paroxysmal events Results The characteristic events were captured in 130(60%) of the 216 patients And out of the 130 patients, 53 had clinical events accompanying epileptiform discharge 73 patients had no epileptiform discharge in both ictal and interictal period Seizure types were defined in 80%of 80 patients with epileptiform discharge, and classification was different from the original 42% of the 64 patients Conclusions Video EEG can record much more epileptiform discharge than routine EEG It should be an effective method in both diagnosis and classification of epilepsy

Objeetive To study the application of template in intensity modulated radiotherapy (IMRT)planning.Methods With Philips Pinnacle3(R) version 7.6c treatment planning system(TPS) platform.a template for left breast cancer's IMRT planning was customized by using the function of scripting.Ten patients received IMRT alone after breast-conserving surgery were selected,two IMRT plans were designed for each patient:individual plan and the plan based on the template.Dose parameters of target,including conformal index(CI)and homogeneity index(HI),dose-volume histogram parameters,including V30,V20 of lung and cardiac,and the planning time were compared respectively.Results Of the two IMRT plans based on the template method and individual method.The average time of design an IMRT plan were 7±0.82 min and 36.5±6.84 min(t=13.55,P=0.000),the PTV average CI were 0.851± 0.015 and 0.845±0.032(t=0.59,P=0.560),the PTV average HI were(13.2±1.3)%and(14.4±1.6)%(t=1.804,P=0.08),the lung average V30 were(8.80±0.53)%and(8.31±0.74)%(t= cardiac average V30 were(9.89±1.14)%and(9.66±1.43)%(t=0.41,P=0.688).Conclusion For the tumor with the same location and stage,the application of template can simplify planning process and reduce working time,which was worthy of expansion.