Objective To investigate the protective effect and mechanism of naloxone preconditioning ( NP) for rats with cerebral hemorrhage. Methods Totally 30 healthy male Sprague Dawley ( SD) rats were randomly separated into Sham operatopm group,intracerebral hemorrhage ( ICH) and NP treatment groups ( n =10) . Before the establishment of rat cerebral hemorrhage model,rats in the NP group were given an intraperitoneal injection of naloxone hydrochloride ( 2. 0 mg/kg) ,and those of ICH group received an injection of normal saline at same volume. Then,the ICH model was made by injecting 0. 4 IU collagen Ⅶ into caudate nucleus while. The brain tissue samples around cerebral hemorrhagic focus after ICH were collected. RT-PCR and Western blotting were used to detect the transcriptional level and protein level of aquaporin protein 4 ( AQP4) in the brain tissue,respectively. Meanwhile,immunohistochemistry was employed to determine the expression of matrix metalloproteinase 9 ( MMP-9) in the rat brain tissue. Results Brain water content in ICH group was larger than that in Sham group ( P

Objective To evaluate the curative effect of layering fibula feet cushion to prevent deep venous thrombosis(DVT) after total hip replacement(THA).Methods 100 patients who underwent THA were divided into two groups at random.The research group was applied layering fibula feet cushion while the control group was applied general feet cushion.VAS,the occurrence rate of DVT,disappearance time of feet swelling and Harris hip function score were used to evaluate the curative effect.Results In according to these four observation indexes,the score of the research group was better than the control group.The effective rate of the research group was 96％,while the control group was 82％.There was a significant difference between the two groups.Conclusions The layering fibula feet cushion can effectively prevent deep venous thrombosis after total hip replacement.It can be generalized and applied in clinical nursing.

To explore the mechamisms of bactericidal neutralizing endotoxin peptide(BNEP), a synthetic peptide mimicking bactericidality/permeability-increasing protein (BPI). The affinities of BNEP for LPS and Lipid A were determined with biosensor technology, and the ability of BNEP neutralizing LPS in vitro was tested by quantitative limulus amoebocyte lysate assay. The results showed that BNEP had high affinities for both LPS and Lipid A. The Kd value for LPS was at the level between 25.8 and 48.8nmol/L and for Lipid A from 11.8 to 21.8nmol/L. When 8?g/ml of BNEP was used, it could completely neutralize the concentration of 2ng/ml of LPS in vitro. It is concluded that BNEP has high binding affinities for both LPS and Lipid A. Our results also suggest that the binding site of LPS is at the glucosaminyl-?1'-6-glucosamine disaccharide of Lipid A. The binding activity of BNEP for LPS is in accord with its neutralizing activity for LPS.

Objective To evaluate the effectiveness of "dispersing lung-qi and relieving asthma, warming Yang to induce diuresis" method on CPHD patients during acute period with peripheral edema and observe the effect on ADH. Methods The CPHD patients during acute period with peripheral edema were randomly divided into treatment group and control group. The treatment group received Chinese medicine adding to regular western medicine, the principle is dispersing lung-qi and relieving asthma, resolving phlegm to stop coughing, warming Yang to induce diuresis. The control group received placebo adding to regular western medicine. Both of the groups were treated for 14 days. The effect observed indexes were as follows:general curative effect, integral of main symptoms of TCM, integral of western medical signs, serum electrolyte, etc. Mechanism index was about ADH. Results The treatment group had better effects than the control group on the general curative effect, reducing integral of symptoms and signs, rectifying electrolyte and acid-alkaline-balance disturbances, etc. The safety analysis of two groups had no significant abnormality. Meanwhile, the treatment group had more superiority than the control group on reducing serum ADH. Conclusion The comprehensive treatment of traditional Chinese and western medicine probably has more advantage in increasing the general effect, improving symptoms and signs, rectifying electrolyte and acid-alkaline-balance disturbances, etc. This therapeutic method can restrain secretion of ADH. It indicates that restraining secretion of ADH maybe part of the mechanism of using "dispersing lung-qi and relieving asthma, warming Yang to induce diuresis" method to treat CPHD patient during acute period with peripheral edema.

AIM To observe the effect of phosphonoformic acid (PFA) in different concentration on vascular calcification induced by elevated phosphate. METHODS The cell culture of bovine aortic smooth muscle were performed. Calcium deposition in different phosphate and PFA concentrations were determined by O cresolphthalein complexone method, and osteocalcin expression by radioimmunity and RT PCR. RESULTS Compared to Pi 1 5 mmol?L -1 group,bovine smooth muscle cells (BSMC) cultured in medium containing Pi 2 0 mmol?L -1 phosphate level increased calcium deposition 〔On day 6, (77 187?11 692) mg?g -1 Pro versus (25 768?1 750) mg?g -1 Pro, P

Objective To investigate the mechanism of high phosphate - stimulated calcification in vascular smooth muscle cell. Methods The cell culture of bovine aortic smooth muscle was performed. Osteocalcin (OC) mRNA expression and calcium deposition in different phosphate concentrations [normal phosphate (Pi 1. 5mmol/L), high phosphate (2.0mmol/L)] were determined by radioimmunoassay, RT-PCR, and 0-cresolphthalein complexone method respectively. Results After 72 hours,high phosphate treatment of smooth muscle cells(SMC) enhanced the expression of osteocalcin protein [Pi 2. 0 mmol/L versus Pi 1. 5 mmol/L: (15. 03?2. 60) pg/u-g protein versus (2. 98 ?0.84) pg/ug protein, P

Middle East respiratory syndrome coronavirus (MERS-CoV) has caused outbreaks of SARS-like disease with 35% case-fatality rate, mainly in the Middle East. A more severe outbreak of MERS occurred recently in the Republic of Korea, where 186 people contracted the infections, causing great concern worldwide. So far, there has been no clinically available drug for the treatment of MERS-CoV infection. The potential drugs against MERS-CoV mainly consist of monoclonal antibodies, peptides and small molecular agents. Small molecular agents have an advantage of easier synthesis, lower cost in production and relatively higher stability. There is better chance for those candidates to gain a quick development. This article reviews the progress of developing small molecular MERS-CoV agents.

Objective To study the clinical features of Crohn's disease(CD)at different location,I.e.small intestine.colon and ileocolon,to facilitate the knowledge of the disease.Methods Data of 103 patients,hospitalized to our department from 2000 to January 2008,were retrospectively analyzed,including general status,clinical manifestations,laboratory findings and pathological changes.Results The cohort included 70 males and 33 females,with the age when the diagnosis was confirmed ranging from 12 to 70,with a peak at 20-29 yr.The location of the main lesion was at small bowel(L1)in 39(38%),at colon (L2)in 16(16%)and at ileum-colon(L3)48(46%).There was no significant difference between each group,regarding the age of onset or the when the diagnosis was confirmed.The incidence of intestinal obstruction was higher in L1 patients than that in L3(P＜0.05).In group L1,12(30.8%)were diagnosed by capsule endoscopy,and 17(44%)were confirmed by colonoscopy.Diagnostic rates of L2 and L3 with reference to clinical manifestations.combined with colonoscopy and pathology were 87.5%and 83.3%,respectivelv.In this cohort,there were 23(22.3%)mild cases,58(56.3%)moderate cases and 22 (21.4%)severe cases,according to simplified CD activity index(CDAI).The rate of severe case in L3 was 59.0%(13/48),which was significantly higher than that in L1(P＜0.05).Conclusion In patients with CD,L1 is characterized by delayed diagnosis and need of emergent surgery,while L3 is featured with extensive involvement.severe complications and systemic manifestations.Severe case is common in 13 patient,capsule endoscopy and Colonoscopy are important in early detection of the disease to decrease operation rate and delay the time of first operation.

Objeictivie To investigate the diagnostic value of anti-mutated citrullinated vimentin (anti-MCV) antibody, anti-cyclic citrullinated peptide (anti-CCP) antibodies and glucose-6-phospha-teisomerase (GP1) for rheumatoid arthritis (RA). Methods Anti-MCV antibody, GPI and anti-CCP antibody were detected in serum samples of 109 RA patients, 24 non-RA rheumatic diseases patients and 19 healthy blood donors. The sensitivity and specificity of these parameters for the diagnosis of RA were analyzed. Results Both the positive rate and average cut off concentration of anti-MCV and GPI in RA were higher than those of non-RA rheumatic diseases or healthy controls (P<0.05). A significant difference was found between anti-MCV and GPI in RA patients. The most sensitive and specific parameter in RA was anti-MCV (99.1%) and anti-CCP (90.7%) respectively, but, when anti-MCV combined with anti-CCP, or GPI or anti-CCP and GPI, the specificity could be up to 98.1%. Coniclusions Anti-MCV, anti-CCP and GPI alone or in combination may be valuable parameters for the diagnosis of RA.

Objective To evaluate the clinical characteristics of epithelioid trophoblastic tumor (ETT).Methods Six cases of ETT treated in Women's Hospital,School of Medicine,Zhejiang University from 2005 to 2007 were retrospectively analyzed,together with a literature review.Results Six cases of ETT were diagnosed pathologically after surgery.The age of patients ranged from 27 to 46 years.The most common presentation was abnormal vaginal bleeding(5/6).The preceding gestational events were hydatidiform mole in 1 case,abortion in 2 cases,and term delivery in 3 cases.The interval between the preceding gestation and the diagnosis of ETT ranged from 15-48 months.The serum human chorionic gonadotropin(hCG)level was 46-121 147 IU/L.Four cases presented with metastasis,including lung metastasis in all of the 4 cases,liver metastasis in 1 case,and pancreas metastasis in another 1 case.The main therapies were surgery combined with chemotherapy.All of the 6 cases received total abdominal hysterectomy.and 1 case also had lung lobectomy.One ease had a recurrence but refused any treatment again,and was lost to follow up;the therapy of 1 case unfinished;another 4 cases were without evidence of disease 9 to 19 months after surgery.Condusions The confirmation of ETF diagnosis is difficult before surgery.Surgical management is mostly recommended in ETT. The role of chemotherapy in ETT is not clear yet.