1.Introduction about writingof patent applications for biochemical analysis field
Chinese Journal of Medical Science Research Management 2014;27(6):683-685
The paper briefly introduces the judging points about description fully disclosed and claims supported bydescription.Problems about patent writing are analyzed combining with specific cases,and some guidlinesare provided for authors in universities and scientific research institutions.
2.Advances in microbial genome reduction and modification.
Chinese Journal of Biotechnology 2013;29(8):1044-1063
Microbial genome reduction and modification are important strategies for constructing cellular chassis used for synthetic biology. This article summarized the essential genes and the methods to identify them in microorganisms, compared various strategies for microbial genome reduction, and analyzed the characteristics of some microorganisms with the minimized genome. This review shows the important role of genome reduction in constructing cellular chassis.
Genes, Essential
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genetics
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Genetic Engineering
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Genome, Microbial
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genetics
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Synthetic Biology
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methods
3.Probe into and Practice in the Reform of Bilingual Teaching
Chinese Journal of Medical Education Research 2003;0(04):-
Starting from the current situation and problems in bilingual teaching, this article probes into the difficulties in promoting bilingual teaching in undergraduate education and gives the view of "teacher is the base, student is the subject and teaching material is the carrier". Combining the experience of bilingual teaching in Pharmaceutical University of China, the authors give some beneficial suggestions in the policy guidance, teaching material construction, teaching performance and teachers training.
4.Study of carotid artery ultrasonography in young and middle-aged patients with cerebral infarction
Journal of Clinical Neurology 1995;0(04):-
0.8 mm) of carotid artery in patient group (42.3%) were more than those in control group (7.4%)((P45 yrs).Conclusion Cerebral infarction in young and middle-aged patients is closely related to atherosclerosis of carotid artery.
5.Time course and neural basis of early visual perception for facial emotions
Xiaoyan LIAO ; Qun WANG ; Xiaoyuan ZHANG
Chinese Journal of Behavioral Medicine and Brain Science 2016;25(11):1046-1050
The complex multidimensional visual patterns of facial expression is particularly well sui ted for studying perception and the processes underlying perceptual integration of component parts into a meaningful whole.With the high resolution in milliseconds,event-related potential (ERP) may be an excellent technique to track the time course of cognitive processing for facial emotions.Facial affective stimuli elicit particular ERP components involved in early processing of facial expressions,including:early sensory processing of visual stimuli:the P100;early structural encoding of facial features:the N170;second-order sustained activities necessary for consequent deeper processing:the posterior P2 and decoding of facial features to identify emotional content:the N250.We reviewed above mentioned ERP components respectively,in order to add the knowledge about ERP components as indicators of neural dynamics of facial emotion processing,and to provide a point of reference for further exploration on facial emotion processing that may be useful for understanding human cognition development and pathology.
6.Research in reliability and validity of the Chinese version of conditions of learning effectiveness questionnaire
Yanhui LIU ; Xiaoyuan CAO ; Shanshan WANG
Chinese Journal of Practical Nursing 2011;27(22):73-75
Objective To assess the reliability and validity of the Chinese version of Conditions of Learning Effectiveness Questionnaire (CLEQ). Methods To translate and revise the Chinese version of CLEQ. A total of 176 baccalaureate nursing students were surveyed by the Chinese version of CLEQ and the results about the reliability and validity of the Chinese version of CLEQ were assessed. Results Cronbach's α of the total Chinese version of CLEQ was 0.956. Cronbach's α of four subscales were all over 0.70. The internal consistency was good. The reliability of Split half was 0.926 and retest reliability was 0.869. CVI of the four subscales were 0.91~1.00. The average CVI of the scale was 0.94. There were significant relationships among subscales. The correlation coefficient was 0.691~0.829. Four common factors were found through factor analysis, which explained the percent of 61.63% rotation sums of squared loadings. Conclusions The reliability and validity of the Chinese version of CLEQ were good, so it could be used to evaluate the level of clinical learning environment among nursing students.
7.Application of combined therapy based on the principle of machanical ventilation in acute respiratory failure induced by aspiratory pneumonia
Xiaoyuan SHEN ; Yunchao WANG ; Jingyong LUO
Chinese Journal of Primary Medicine and Pharmacy 2008;15(12):2004-2005
Objective To explore the application of combined therapy based on the principle of machanical ventilation in acute respiratory failure by aspiratory pneumonia.Methods 32 cases of acute respiratory failure induced by aspiratory pneumonia were collected by retrospective analysis.Results With the combined therapy based on the principle of machanical ventilation,30 patients take a favorable turn.Conclusion With the therapy of anti-infection,energetic respiratory support,nutrition support etc,the patients'prognosis suffering from acute respiratory failure by cerebral apoplexy with aspimtory pneumonia ale fine.
8.Reconstruction of ACL with free autogenous B-PT-B grafts
Xing TENG ; Manyi WANG ; Xiaoyuan HUANG
Chinese Journal of Orthopaedics 2001;0(08):-
Objective To investigate the characteristics of grafts after reconstruction of ACL with autogenous bone-patellar tendon-bone(B-PT-B)grafts. Methods Reconstruction of anterior cruciate ligament with free B-PT-B autograft fixed with interference screw was performed on 39 adult dogs. Mechanical testing was conducted on the grafts by translating the tibia anteriorly with the knee in 90 degrees of flexion, until complete failure. Failure site, cross sectional area, strength, stiffness and stress were documented. After decalcification and HE staining, the transformation of the intra-tunnel and intra-articular ligaments was evaluated, and the histological observation was conducted for the healing in the bone-tunnel, patellar tendon-tunnel and bone-patellar tendon interface. Results Union occurred in the bone-tunnel interface at 4th week. The patellar tendon-tunnel interface was linked by Sharpey's like fibers at 52nd week, the cement line and the tendency of chondrocyte lining were found. Mechanical testing of graft revealed that the rupture always occurred at mid-portion of ligaments instead of pulling-out from the tunnel under tension stress. Compared with the control group, the strength, stiffness and stress of the graft were 38%, 58% and 50% of normal anterior cruciate ligament at 52nd week respectively. Conclusion The union in the bone-tunnel interface occurs at 4th week, much earlier than the healing of the patellar tendon-tunnel interface. The healing of the tendon to tunnel presents to be indirect insertion at 52nd week with alignment of chondrocytes, which signifies the still ongoing of healing process at the interface. After fixation with interference screw for 4 weeks, the tendon proved to be the weakest part in bone-tendon-bone complex. No need to worry about the biomechanical character of the new insertion site during rehabilitation activities. The attachment fixed by interference screw is stable not only immediately after surgery but also during the healing of the insertion and the transformation of ligament. One year after reconstruction, the mechanical quality of the grafted tendon still remains inferior to that of normal anterior cruciate ligament in spite of similar histological structure. Protective device should be recommended during active activity.
9.Effect of radix paeoniae rubra on expression of heme oxygenase and inducible nitric oxide synthesis in lipopolysaccharide-induced acute lung injury in rats
Zhongyuan XIA ; Chang CHEN ; Xiaoyuan WANG
Chinese Journal of Trauma 2003;0(09):-
Objective To investigate the effect of radix paeoniae rubra (RPR) on expression of heme oxygenase (HO-1) and inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) and explore its protective mechanism. Methods Forty Wistar rats were randomly and equally divided into five groups, ie, control group, LPS group, RPR treatment group, RPR prevention group and Hemin group. Arterial blood was drawn for blood gas analysis. Models of endotoxin-induced ALI were used to observe the protein content, the ratio of neutrophiles in bronchoalveolar lavage fluid (BALF), the malondialdehyde (MDA) content in lung and the activities of serum NO. Expression of HO-1 and iNOS in rat lung tissue was detected by immunohistochemistry and morphometry computer image analysis. The histological change of lung were observed under light microscope. Results Compared with control group, expression of HO-1 and iNOS was markedly increased (P
10.Comparison of three techniques monitoring depth of anesthesia during brain surgery
Xiaoyuan LIU ; Yuming PENG ; Baoguo WANG
Chinese Journal of Anesthesiology 1994;0(05):-
Objective To evaluate the performance of bispectral index (BIS),auditory evoked potential index (AEPI) and cardiovascular response to electrical tetanus stimulus (ETS) for monitoring depth of sedation induced by propofol , analgesia produced by fenanyl and depth of isoflurane anesthesia. Methods Twenty ASA Ⅰ or Ⅱ patients of both sexes (11 males,9 females) aged 18-56 yr, weighing 50-80 kg undergoing elective brain surgery were studied. The patients were unpremedicated. The baseline BIS, AEPI, BP(SP, DP) and HR were measured before anesthesia. Propofol was infused at 0.25 mg?kg-1?min-1 and the infusion was stopped when the total amount of propofol infused reached 5 mg?kg-1 at the end of 20 min infusion. Fentanyl 2 ?g?kg-1 was then given i.v. Three minutes later the patients were intubated and mechanically ventilated. Tracheal intubation was facilitated by vecuronium 0.1 mg?kg-1. Anesthesia was maintained with isoflurane. Depth of sedation was measured using OAA/S scale during propofol infusion. ETS was given at the following time points: when the amount of propofol infused reached 2.5, 3.0, 3.5, 4.0, 4.5, 5.0 mg?kg-1,2 min after fentanyl administration and at 0.7, 1.0, 1.3 MAC isoflurane (end-tidal isoflurane concentration). BIS and AEPI values before and after ETS and changes in BP and HR (ABP, AHR) after ETS and the time for BP and HR to return to the pre-ETS value in 50% and 90% of the patients (TBP50%,TBP90%,THR50%,THR90% ) were recorded. Results BIS and AEPI were positively correlated with sedation depth. There were no significant changes in BIS and AEPI after ETS, but SP, DP and HR increased significantly after ETS( P