BACKGROUND: Studies have shown that 160 mg/L tramethylpyrazine (TMP) can inhibit the proliferation of endothelial cells cultured in vitro,but whether TMP also inhibits vascular endothelial growth factor (VEGF)induced proliferation of vascular endothelial cells remains unkown. OBJECTIVE: To study the effect of rat serum containing TMP on vascular endothelial cell proliferation and its proliferation induced by VEGF. DESIGN: Randomized controlled experiment. SETTING: College of Traditional Chinese Medicine, Chongqing University of Medical Sciences. MATERIALS: The experiment was carried out in the Institute of Pediatrics, Chongqing University of Medical Sciences from March, 2002 to March, 2003 using human umbilical vein endothelial cell (HUVEC) line ECV304 and 30 female Wistar rats. METHODS: Thirty rats were randomly divided into 3 equal groups to receive intraperitoneal TMP injection at 143.0 mg/kg and 71.5 mg/kg and 0.8 mL normal saline (blank control group). All the injections were performed once a day for consecutive 7 days, then blood was collected from the intraperitoneal artery and diluted to 20％, 10％, and 5％ in triplicate.Effect of TMP-containing serum on the proliferation of ECV304 cells was observed by means of in vitro culture and 3H-TdR incorporation as well as methyl thiazolyl tetrazolium (MTT) methods. MAIN OUTCOME MEASURES: ① Effect of TMP-containing serum on proliferation of ECV304 cells in in vitro culture and VEGF-induced proliferation of the cells.RESULTS:All the 30 rats survived the experiment without losses. The absorbance (A) and scintillation counting (minute-1) of the cells were significantly lower in 143.0 mg/kg TMP group than those in the control group treated with rat serum at the dilution of 5％ (0.720±0.024 vs 0.816±0.068,3340.45±567.7 vs 5120.84±301.49), 10％ (0.630±0.017 vs 0.798±0.015,2430.06±265.98 vs 5225.83±100.10), and 20％ (0.765±0.027 vs 0.823±0.031,3570.45±130.52 vs 5256.82±183.18), and those in 71.5 mg/kg TMP group were also significantly lower than those in the control group after treatment with the serum of 10％ (0.775±0.023, 4571.14±275.39) and 20％(0.749±0.012, 3287.25±144.82). In the experiment evaluating the effect of TMP serum on VEGF-induced proliferation of ECV304 cells, the A value and scintillation counting in the 143.0 mg/kg TMP group were significantly lower than those in the control group after the cells were treated with the serum of 5％ (0.726 ±0.004 vs 0.964 ±0.004, 5760.46 ±49.64 vs 9821.82±128.05), 10％ (0.712±0.004 vs 0.933±0.014, 5024.48±100.57 vs 9052.76±65.19), and 20％ (0.717±0.003 vs 0.924±0.004, 5405.45±140.90vs 9197.07±169.92], and those of the cells treated with 71.5 mg/kg TMP serum of 10％ and 20％ were also significantly lower than those in the control group (0.703±0.005 and 7526.47±169.21 for 10％ serum, and 0.693±0.006 and 5720.09±279.03 for 20％ serum). CONCLUSION: The sera at the concentrations of 5％,10％, and 20％from rats treated with 143 mg/kg TMP and at higher concentrations from 71.5 mg/kg TMP-treated rats can inhibit the proliferation of ECV 304 in in vitro culture as well as VEGF-induced proliferation of the cells.

Chronic active Epstein-Barr virus infection( CAEBV) is an undefined illness, which is often characterized by severe. chronic, or recurrent infectious mononucleosis-like syndrome, such as fever, hepatosplenomegaly, persistenthepatitis and extensive lymphadenopathy . Patients with CAEBV have high viral loads in their penpheral blood and/ or an unusual pattem of EBV-related antibodies. It is an intractable disease with a poor prognosis. Patients with CAEBV have high mortality from hepatic failure, opportunistic infection, or hemophagocytic lymphohistiocytosis. A definite treatment has not been established . There is accumulating evidence that the clonal expansion of EBV-infected T or natural killer cells play a key role in the pathogenesis of CAEBV.

Drug pair is the basic unit of compatibility of formulas.Through selecting the prescriptions of treating diseases of the liver in "Treatise on Cold Pathogenic Diseases" and "Synopsis of Golden Chamber",we,according to therapeutic methods,classify the commonly used drug pairs in treatment of hepatic disease of ZHANG Zhong-jing into eight kinds:regulating the flow of liver-qi,promoting blood circulation by removing blood stasis,dispersing pathogenic wind and calming liver wind,removing jaundice,clearing away liver-heat,expelling liver-cold and warming the liver,nourishing the liver,and harmonizing liver-spleen.And we try to find the compatibility law.

Objective To observe the effects of rat tetramethylpyrazine (TMP)-containing serum on the proliferation of human liver cancer cells Hep G2. Methods Thirty SD male rats were divided into three groups randomly and the serum was collected after ip TMP with the dosage of 143.0, 71.5 mg/kg or NS 0.8 mL to prepare the TMP-containing serum in large- and small-dose groups and NS group as well. A reversed-phase high performance liquid chromatography (RP-HPLC) method was used to determine the TMP concentration in rat serum. Human liver cancer cells Hep G2 was treated with rat TMP-containing serum for 48 h. Inhibition of the TMP-containing serum on proliferation of Hep G2 was detected by MTT assay. Results The serum of large dose TMP (20%, 10%, and 5%) and small dose TMP (20% and 10%) could obviously inhibit Hep G2 multiplication (P

The growth hormone(GH)-insulin-like growth factor(IGF)-I axis is an important modulator of growth and development.In addition to the classical role as endocrine hormones,the components also regulate a wide range of biological functions through paracrine and autocrine mechanisms.Their potent mitogenic and anti-apoptotic effects play a critical role in the regulation of rapidly renewing epithelial cell populations such as those found in the colon.Recent epidemiological evidence from acromegaly patients suggests an association between inappropriate regulation of the GH-IGF-I axis and the development of colorectal cancer.However,the molecular mechanisms and signalling pathways responsible are only beginning to be unravelled,as are the relative contributions of the endocrine and autocrine or paracrine effects.

Objective To study the distribution and changes of the purulent meningitis in gender,age,region,season and pathogens in children during the past 20 years.Methods All the basic clirical features of purulent meningitis patients less than 17 years old in Children's Hospital of Chongqing Medical University from Jan.1993 to Jan.2013 were analyzed retrospectively.A total of 1 855 patients were divided into 2 groups according to their date of admission.Group A included 666 patients admitted from Jan.1993 to Dec.2002,and group B included 1 189 cases admitted from Jan.2003 to Jan.2013.Then,they were divided into 5 groups according to their ages,including neonate group,＞ 1 month-1 year old aoup,＞ 1-3 years old group,＞ 3-10 years old group,＞ 10-17 years old group.Results Of the 1 855 cases,there were 1 054 cases (56.82％) admitted in the spring and summer,and 1 123 cases (60.54％) were younger than 1 year of age.The ratio of male to female cases was 1.82 to 1.00 and that of country patients to urban patients was 1.40 to 1.00.The distributions were female patients (443 cases,37.26％),urban patients (517 cases,43.48％),and the ratio of patients admitted in the autumn (265 cases,22.29％) and patients younger than 1 year of age (606 cases,50.97％) in group B,which were significantly higher than those [215 cases (32.28％),256 cases (38.44％),102 cases (15.32％),272 cases (40.84％)] of group A (x2 =4.62,4.47,25.51,25.52,all P ＜ 0.05).The predominant isolated bacteria from cerebrospinal fluid were coagulase negative staphylococcus (43/196 cases,21.94％),Streptococcus pneumoniae (32/196 cases,16.33％),Neisseria meningitidis (17/196 cases,8.67％) and Escherichia coli (15/196 cases,7.65％).Coagulase negative staphylococcus and Escherichia coli both accounted for higher rates of pathogenic bacteria in 1 year old than that of pathogenic bacteria more than 1 year old (x2 =4.57,14.37,all P ＜ 0.05),while Streptococcus pneumoniae and Neisseria meningitidis both had higher rates of pathogenic bacteria more than 1 year old and more than 3 years old,respectively (x2 =14.93,19.76,all P ＜ 0.05).Onfly cases in group B with Streptococcus pneumoniae were higher than those in group A (x2 =11.78,P ＜ 0.05),while the other common bacteria in each age group were of no difference between group A and group B.Conclusions There had been some changes in the epidemiological distribution of purulent meningitis during the past 20 years.The main risk factors were male in gender,patients in rural areas,onset in the spring or summer and infants in early age.The predominant isolated bacteria from cerebrospinal fluid were some opportunistic pathogens,including coagulase negative staphylococcus,Streptococcus pneumoniae,and Escherichia coli.Streptococcus pneumoniae meningitis was increased during the past 10 years.

Objective To evaluate the life quality of patients with nasopharyngeal carcinoma after intensity modulated radiotherapy and chemotherapy. Method Using phenomenological research method, we interviewed 10 patients repeatedly to explore their somatization, psychological changes as well as the effect on quality of life. Result The life quality of patients with nasopharyngeal carcinoma after intensity modulated radiotherapy and chemotherapy were affected tremendously , characterized by intense psychological stress, side effects from radiochemotherapy, hunger for emotional support, heavy burden of medical expenses, and the change of family and work roles. Conclusion Nursing staff should understand the mental feelings of patients, and provide supports for the patients psychologically, physically, from the economic, family and social points of view, so as to improve the life quality and physical and mental health of patients.

[ ABSTRACT] AIM: To discuss the relevance between the pathogenesis of diabetic gastroparesis and the large-conductance calcium-activated potassium channels ( BKCa ) in gastric smooth muscle cells.METHODS:The SD rats were randomly divided into control group and model group.The gastric smooth muscle cells of the SD rats were enzymatically iso-lated in a low calcium solution containing papain.The current was recorded by patch clamp single channel recording tech-nique.The expression of KCNMA and KCNMB1 were observed by the method of immunohistochemistry.RESULTS:The value of BKCa single channel conductance was (220.10 ±10.90) pS;the channels had distinct voltage dependent and cal-cium dependent characteristics.In outside-out patch (Vm =+30 mV), the activation of BKCa was blocked by 200 nmol/L IbTX completely.Compared with control group, the open probability and amplitude of current in model group significant-ly increased, while the mean open time and mean close time significantly decreased.Compared with control group, the ex-pression of KCNMB1 in model group was significantly increased.CONCLUSION: Up-regulation of β1-subunit and in-crease in BKCa functional activities may be associated with diabetes gastroparesis in rats.

OBJECTIVE:To study the effect of ligustrazine injection(LTZ)on DNA synthesis of vascular endothelial cell.METHODS:In vitro cultured cell line of human umbilical vein endothelial cells,ECV304,were adopted to measure the ef?fects of LTZ on DNA synthesis by 3 H-TdR incorporation.RESULTS:LTZ could inhibit DNA synthesis of ECV304in a dose-dependent manner.CONCLUSION:The mechanism of anti-angiogenesis of LTS might be associated with inhibiting DNA synthesis of vascular endothelial cell.

Objective To investigate the application of MR 3D-FLASH dynamic enhanced scanning in the breast diseases. Methods 108 women (35～76 years old,mean,47 years) with breast diseases confirmed by pathology were examined by using Siemens 1.0T superconducting MRI and double breast circles.Plain MR imaging performed first,then contrast-enhanced MR scans were followed with 3D FLASH sequence,and the images were subtracted.Results Among 108 cases,62 cases were benign and 46 cases were breast cancer,the diagnosing accurate rate of MR imaging was 81.5% compared with pathology pre-operation.Conclusion 3D-FLASH enhanced MR scan is the most valuable sequence in diagnosing breast lesions.