Chronic active Epstein-Barr virus infection( CAEBV) is an undefined illness, which is often characterized by severe. chronic, or recurrent infectious mononucleosis-like syndrome, such as fever, hepatosplenomegaly, persistenthepatitis and extensive lymphadenopathy . Patients with CAEBV have high viral loads in their penpheral blood and/ or an unusual pattem of EBV-related antibodies. It is an intractable disease with a poor prognosis. Patients with CAEBV have high mortality from hepatic failure, opportunistic infection, or hemophagocytic lymphohistiocytosis. A definite treatment has not been established . There is accumulating evidence that the clonal expansion of EBV-infected T or natural killer cells play a key role in the pathogenesis of CAEBV.

BACKGROUND: Studies have shown that 160 mg/L tramethylpyrazine (TMP) can inhibit the proliferation of endothelial cells cultured in vitro,but whether TMP also inhibits vascular endothelial growth factor (VEGF)induced proliferation of vascular endothelial cells remains unkown. OBJECTIVE: To study the effect of rat serum containing TMP on vascular endothelial cell proliferation and its proliferation induced by VEGF. DESIGN: Randomized controlled experiment. SETTING: College of Traditional Chinese Medicine, Chongqing University of Medical Sciences. MATERIALS: The experiment was carried out in the Institute of Pediatrics, Chongqing University of Medical Sciences from March, 2002 to March, 2003 using human umbilical vein endothelial cell (HUVEC) line ECV304 and 30 female Wistar rats. METHODS: Thirty rats were randomly divided into 3 equal groups to receive intraperitoneal TMP injection at 143.0 mg/kg and 71.5 mg/kg and 0.8 mL normal saline (blank control group). All the injections were performed once a day for consecutive 7 days, then blood was collected from the intraperitoneal artery and diluted to 20％, 10％, and 5％ in triplicate.Effect of TMP-containing serum on the proliferation of ECV304 cells was observed by means of in vitro culture and 3H-TdR incorporation as well as methyl thiazolyl tetrazolium (MTT) methods. MAIN OUTCOME MEASURES: ① Effect of TMP-containing serum on proliferation of ECV304 cells in in vitro culture and VEGF-induced proliferation of the cells.RESULTS:All the 30 rats survived the experiment without losses. The absorbance (A) and scintillation counting (minute-1) of the cells were significantly lower in 143.0 mg/kg TMP group than those in the control group treated with rat serum at the dilution of 5％ (0.720±0.024 vs 0.816±0.068,3340.45±567.7 vs 5120.84±301.49), 10％ (0.630±0.017 vs 0.798±0.015,2430.06±265.98 vs 5225.83±100.10), and 20％ (0.765±0.027 vs 0.823±0.031,3570.45±130.52 vs 5256.82±183.18), and those in 71.5 mg/kg TMP group were also significantly lower than those in the control group after treatment with the serum of 10％ (0.775±0.023, 4571.14±275.39) and 20％(0.749±0.012, 3287.25±144.82). In the experiment evaluating the effect of TMP serum on VEGF-induced proliferation of ECV304 cells, the A value and scintillation counting in the 143.0 mg/kg TMP group were significantly lower than those in the control group after the cells were treated with the serum of 5％ (0.726 ±0.004 vs 0.964 ±0.004, 5760.46 ±49.64 vs 9821.82±128.05), 10％ (0.712±0.004 vs 0.933±0.014, 5024.48±100.57 vs 9052.76±65.19), and 20％ (0.717±0.003 vs 0.924±0.004, 5405.45±140.90vs 9197.07±169.92], and those of the cells treated with 71.5 mg/kg TMP serum of 10％ and 20％ were also significantly lower than those in the control group (0.703±0.005 and 7526.47±169.21 for 10％ serum, and 0.693±0.006 and 5720.09±279.03 for 20％ serum). CONCLUSION: The sera at the concentrations of 5％,10％, and 20％from rats treated with 143 mg/kg TMP and at higher concentrations from 71.5 mg/kg TMP-treated rats can inhibit the proliferation of ECV 304 in in vitro culture as well as VEGF-induced proliferation of the cells.

Drug pair is the basic unit of compatibility of formulas.Through selecting the prescriptions of treating diseases of the liver in "Treatise on Cold Pathogenic Diseases" and "Synopsis of Golden Chamber",we,according to therapeutic methods,classify the commonly used drug pairs in treatment of hepatic disease of ZHANG Zhong-jing into eight kinds:regulating the flow of liver-qi,promoting blood circulation by removing blood stasis,dispersing pathogenic wind and calming liver wind,removing jaundice,clearing away liver-heat,expelling liver-cold and warming the liver,nourishing the liver,and harmonizing liver-spleen.And we try to find the compatibility law.

The growth hormone(GH)-insulin-like growth factor(IGF)-I axis is an important modulator of growth and development.In addition to the classical role as endocrine hormones,the components also regulate a wide range of biological functions through paracrine and autocrine mechanisms.Their potent mitogenic and anti-apoptotic effects play a critical role in the regulation of rapidly renewing epithelial cell populations such as those found in the colon.Recent epidemiological evidence from acromegaly patients suggests an association between inappropriate regulation of the GH-IGF-I axis and the development of colorectal cancer.However,the molecular mechanisms and signalling pathways responsible are only beginning to be unravelled,as are the relative contributions of the endocrine and autocrine or paracrine effects.

Objective To observe the effects of rat tetramethylpyrazine (TMP)-containing serum on the proliferation of human liver cancer cells Hep G2. Methods Thirty SD male rats were divided into three groups randomly and the serum was collected after ip TMP with the dosage of 143.0, 71.5 mg/kg or NS 0.8 mL to prepare the TMP-containing serum in large- and small-dose groups and NS group as well. A reversed-phase high performance liquid chromatography (RP-HPLC) method was used to determine the TMP concentration in rat serum. Human liver cancer cells Hep G2 was treated with rat TMP-containing serum for 48 h. Inhibition of the TMP-containing serum on proliferation of Hep G2 was detected by MTT assay. Results The serum of large dose TMP (20%, 10%, and 5%) and small dose TMP (20% and 10%) could obviously inhibit Hep G2 multiplication (P

Objective To evaluate the life quality of patients with nasopharyngeal carcinoma after intensity modulated radiotherapy and chemotherapy. Method Using phenomenological research method, we interviewed 10 patients repeatedly to explore their somatization, psychological changes as well as the effect on quality of life. Result The life quality of patients with nasopharyngeal carcinoma after intensity modulated radiotherapy and chemotherapy were affected tremendously , characterized by intense psychological stress, side effects from radiochemotherapy, hunger for emotional support, heavy burden of medical expenses, and the change of family and work roles. Conclusion Nursing staff should understand the mental feelings of patients, and provide supports for the patients psychologically, physically, from the economic, family and social points of view, so as to improve the life quality and physical and mental health of patients.

Objective:The study is designed to explore the neuroimmunomodulation mediated by 5 HT 3 receptor of hippocampus in rats.Methods:ConA or lipopolysaccharide stimulated splenocytes proliferative responses are measured by TdR incorporation.Results:1 phenylbiguanide(PBG) 1 mg/kg,ip,10 ?g,icv and microinjection in the ventral hippocampus could enhance the splenocyte proliferative responses stimulated by ConA and LPS in mice and rats respectively.But the inhibitory effect on ConA stimulated response was only obtained by bilateral microinfusion of PBG in the dorsal hippocampus,while had no effect on the LPS induced proliferative responses.All of effects on mitogen stimulated splenocyte proliferation could be antiagonized by coadministration of a selective 5 HT 3 antiagonist tropisetron.Conclusion:It suggested that the 5 HT 3 receptors in the hippocampus might mediate neuro immuno modulation in which ventral and dorsal hippocampus have different effects.

[ ABSTRACT] AIM: To discuss the relevance between the pathogenesis of diabetic gastroparesis and the large-conductance calcium-activated potassium channels ( BKCa ) in gastric smooth muscle cells.METHODS:The SD rats were randomly divided into control group and model group.The gastric smooth muscle cells of the SD rats were enzymatically iso-lated in a low calcium solution containing papain.The current was recorded by patch clamp single channel recording tech-nique.The expression of KCNMA and KCNMB1 were observed by the method of immunohistochemistry.RESULTS:The value of BKCa single channel conductance was (220.10 ±10.90) pS;the channels had distinct voltage dependent and cal-cium dependent characteristics.In outside-out patch (Vm =+30 mV), the activation of BKCa was blocked by 200 nmol/L IbTX completely.Compared with control group, the open probability and amplitude of current in model group significant-ly increased, while the mean open time and mean close time significantly decreased.Compared with control group, the ex-pression of KCNMB1 in model group was significantly increased.CONCLUSION: Up-regulation of β1-subunit and in-crease in BKCa functional activities may be associated with diabetes gastroparesis in rats.

NVP-BEZ235 is a newly developed dual phosphatidyl linositol kinase 3-kinase (PI3K) and mammalian target of rapamycin (mTOR) inhibitor.Studies have revealed that NVP-BEZ235 combinate with other drugs were effective in the treatment of lung cancer.NVP-BEZ235 might have a potential application value for the treatment of lung cancer of EGFR mutation subtype and KRAS mutation subtype.