Objective To study the effect and mechanism of gene PBP2 and gene PBP3 in drug resistance of Klebsiella pneumoniae(KP) to different antibacterial agents.Methods A total of 203 strains of KP which isolated from clinical samples in each department in our hospital during the period from April 2014 to April 2015.Strains were identified and antibiotic susceptibility were determined with automatic bacteria identification system,then extracted DNA genome from bacteria,proceeded with electro-phoresis after the product of PBP2 and PBP3 were amplified by PCR,examined genetic expression with RT-PCR,and study their connection to drug-fast of the KP combining results of antibiotic susceptibility.Results In 203 strains of KP,105 strains resistant to β-lactam drug (51.7%),88 strains were sensitive,resistant rate to imipenem and meropenem were 5.4%,3.9% respectively.The PCR amplification products of the electrophoresis bands of PBP2,PBP3 gene,18 strains were negative,185 strains were positive,which was not parallel,there were respectively negative and positive strains among them.There was no significant correlation between PBP2,PBP3 and multiple drug resistance of KP(P=0.295,0.628).PBP2 was not related to the resistance of carbapenem(P=0.637).PBP3 had correlation with carbapenem resistance,but the correlation coefficient was not high(P=0.041,r=0.148).After reverse transcription of PBP2,PBP3,statistically analyze electrophoresis bands of amplification products of carbapenem-resistant KP and 16SrRNA amplification bands of gray gradient ratio,there was no statistical significance(PBP2:P=0.331,PBP3:P=0.383).Conclusion Gene PBP2,PBP3 might not be the necessary reasons of KP being multiple drugs resistant.

Seventy-eight children with anorexia were treated by circling Neibagua, rubbing abdomen and kneading navel, parting-pushing Fuyinyang, pressing Zusanli (ST 36) and pinching spine. Results showed cure in 48 cases, improvement in 27 cases and failure in 3 cases, with a total effective rate of 96.1%.

Objective To investigate the relationship between self-esteem and social anxiety,and the role of putting into perspective played in this relationship in university students.Methods Sample consisted university students of 376 females and 164 males,data were obtained on cognitive emotion regulation questionnaire,social anxiety subscale of the self-consciousness scale,and self-esteem scale.Results Cognitive emotion regulation strategies of putting into perspective is significantly and positively related to self-esteem(r=0.12,P＜ 0.01),it was also significantly and negatively related to social anxiety(r =-0.01,P ＜ 0.05) ; self-esteem was significantly and negatively related to social anxiety(r =-0.35,P ＜ 0.001).Cognitive emotion regulation strategies of putting into perspective played both a moderating(AR2 =0.124,P＜0.05) and mediating role(F=30.717,P＜ 0.01) in the relationship between self-esteem and social anxiety.Conclusion Self-esteem is not only directly but also indirectly associated with social anxiety because of the moderation and mediation model of putting into perspective.

OBJECTIVE:To discuss the curative effects,adverse drug reactions(ADRs)and cost—effectiveness of 3 kinds of nitroimidazole drugs for treatment of trichomonal vaginitis.METHODS:111 cases of trichomonal vaginitis were randomly divided into 3 groups,administered with metronidazol(group A),tinidazole (group B),and ornidazol(group C)respectively,the curative effects and ADRs were observed and cost-effectiveness analysis was made in all groups.RESULTS:The costs in 3 groups were 0.74 yuan,33.6 yuan and 41.2 yuan respectively.The cure rates were 59.4%,81.6%and 97.2%,respectively.The cost-effectiveness ratios were 0.01,0.41,and 0.42 respectively.The increment cost -effectiveness ratios in group B and C were 1.48 and 1.07,as compared with group A.The occurrences of ADRs were 62.2%,18.4%,and11.1%respectively.CONCLUSION:Among 3 regimes,regime C is the preferred one.

Objective To study the management of enterostomy in children.Methods The clinical data of 425 cases of enterostomy in children were analyzed retrospectively.Results The primary diseases of 425 cases underwent enterostomy included:intestinal perforation(119 cases),intestinal necrosis(36 cases),necrotizing enterocolitis(45 cases),congenital megacolon(61 cases),congenital anal atresia with rectourethral fistula(86 cases),and congenital anal atresia with rectovaginal fistula(78 cases).Among the 425 cases,159 cases underwent small bowel enterostomy and 266 cases had colostomy;single-stoma enterostomy was done in 225 cases,and double-stoma enterostomy in 197 cases.Follow-up was acquired in 389 patients,and all the stomas were closed successfully.The closure time of small bovvel enterostomy was 1-3 month(ayerage 1.87 months)later,and of colostomy was 3-12 month(averag 4.95 months)later.Conclusions When an enterostomy is to be adopted in pediatric surgery,a suitable time and enterostomal location should be chosen according to the state of illness.The timing of enterostomy closure is also very important.

Objective Atrophic gastritis, as a pre-cancerous condition of gastric cancer, is developed from non-atrophic gastritis.The aims of this study was to compare the gene expression profiles between atrophic gastritis and non-atrophic gastritis using cDNA microarray and to explore the molecular mechanisms in the development of atrophic gastritis. Methods Endoscopy and biopsy were performed consecutively in 227 patients (143 male, 84 female, age range between 16-72 years, with average age 48.6 years). Diagnosis of non-atrophic gastritis and atrophic gastritis was made according to histological examination, and 120 patients were in non-atrophic group and 107 in atrophic group. The total RNA was extracted from the biopsy specimens of two groups with Trizol reagent. cDNA microarray consisting 8 464 human genes (HGEC-80s kit) was used, and labeled cDNA with fluorescence probes were hybridized to microarray, and then the comparison of gene expression profiles was made between atrophic and non-atrophic gastritis. Results It was identified that in gene expression profiles of atrophic gastritis there were 165 genes showing a greater than 2-fold increase and 460 genes showing a greater than 50% decrease as compared to non-atrophic gastritis. RT-PCR analysis revealed similar results as in DNA microarray. Conclusions There is different expression in many genes between the gastric mucosal cells of non-atrophic and atrophic gastritis mucosa. Many genes expression profiles are involved in atrophic gastritis that would be of help in further understanding the development of atrophic gastritis.

ObjectiveTo explore the correlations of university students'love style,dysfunctional attitude,and love satisfaction and life satisfaction.MethodsA total of 552 college students were assessed with relationship assessment scale(RAS),dysfunctional attitude scale(DAS),loving style scale(LSS) and life satisfaction index-A (LSIA).Results ( 1 ) The score of RAS of erotic loving style was ( 27.85 ± 4.624 ) significantly higher than the counterparts of the ludus loving style,which was (23.89 ± 4.061 ) (P ＜ 0.01 ).(2) There were significantly positive correlations among eros loving style,storge loving style,agape loving style,pragma loving style,life satisfaction and loving satisfaction,and negative correlations among Ludus loving style,dysfunctional attitude and loving satisfaction.(3)Loving satisfaction could be significantly predicted by dysfunctional attitude,eros loving style,ludus loving style,pragma loving style and storge loving style.ConclusionsLoving style and dysfunctional attitude can significantly predict loving satisfaction.

Objective To evaluate the effects of remifentanil preconditioning on the renal injury induced by limb ischemia-reperfusion (I/R) in rats.Methods Twenty-seven healthy male Sprague-Dawley rats,weighing 200-250 g,were randomly assigned into 3 groups (n =9 each):sham operation group (Sham group),I/R group and remifentanil preconditioning group (RPC group).Limb ischemia was induced by clamping bilateral femoral arteries and veins for 2 h,followed by 24 h reperfusion in I/R and RPC groups.Remifentanil 1.0 μg· kg-1 · min-1 was infused via the caudal vein for 30 min before ischemia in RPC group,while the equal volume of normal saline was given in Sham and I/R groups.Blood sample was taken from the inferior vena cava at 24 h of reperfusion to measure blood urea nitrogen (BUN) and creatinine (Cr) concentrations in serum.The rats were sacrificed and the left kidney was removed for determination of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) contents,cell apoptosis and microscopic examination of pathological changes.Apoptosis index was calculated.Results Apoptosis index and concentrations of BUN and Cr in serum,TNF-α and IL-6 contents,and apoptosis index were significantly higher,and the pathological changes were more severe in I/R and RPC groups than in Sham group.Compared with group I/R,concentrations of BUN and Cr in serum,TNF-α and IL-6 contents,and apoptosis index were significantly decreased,and the pathological changes were attenuated in RPC group.Conclusion Remifentanil preconditioning can attenuate renal injury induced by limb I/R in rats,and inhibition of inflammatory responses and cell apoptosis in kidney may be involved in the mechanism.

Objective To screen out the individuals at high cognitive risk by using the weakest link theory,and explore the characters of the cognitive emotion regulation.Methods 600 undergraduate students were tested with cognitive style questionnaire,general social and academic hassles scale,center for epidemiologic studies depression scale,and cognitive emotion regulation questionnaire.Results There were significant differences between individuals of high and low cognitive risk in self-blame (13.32 ± 2.12) vs (12.45 ± 2.27),rumination,catastrophizing,blaming others,positive refocusing and positive reappraising.Catastrophizing,generality,rumination,positive reappraising,stress,self-blame,and self could predict 34.2％ of depression.Conclusion There are differences between individuals of high and low cognitive risk in cognitive emotion regulation.Catastrophizing,generality,rumination,positive reappraising,stress,self-blame,and self are the main predictors of depression.

A simple and efficient method for the determination of organotin compounds ( OTCs) in biota by GC-MS coupling with sodium tetraethylborate ( NaBEt4 ) derivatization was established. The results showed that recoveries for each OTCs spiked in blank freeze-dryed biological samples ( 0 . 1 g ) following extraction (15 mL 0. 035 mol/L methanolic HCl solution for 1 h ) and derivatization (2 mL contained internal standard n-hexane and 0. 5 mL of 2% (m/V) NaBEt4 solution for 30 min) were above 80%. The method is fast and easy to operate for the determination of OTCs in complex biological samples. The minimum limit of detection was about 0. 01 μg/L, and relative standard deviation values were within 10% for three parallel samples.