Objective:To investigate the expression of sonic hedgehog(SHH) in gastric cancer,analyze the relationship with clinic pathological features,and discuss the clinical significance.Methods:Immunohistochemistry SP method was used to detect the expression of SHH in 60 cases of gastric cancer,and the relationship between protein expression and clinic pathological features was analyzed.Results:The positive expression rates of SHH was 68.3%(41/60)in gastric cancer.The expression rates of SHH was correlated with depth of invasion,lymph node metastasis,and TNM stage(P

Objective: To investigate the role of intraoperative ultrasound(IOUS) and appropriate approach in local resection for the central small hepatocellular carcinoma(sHCC) which is deeply situated within the parenchyma and adjacent to the hepatic veins. Methods: under IOUS guidance, local resection was carried out for 8 cirrhotic patients with central sHCC which was deeply located and adjacent to hepatic veins by approach of splitting or unroofing superficial hepatic parenchyma. Results: All tumors were precisely localized by IOUS and excised successfully. Except for injury to central hepatic vein in 1 case, no other injury occurred to the hepatic veins in the remaining 7 cases. There was no postoperative mortality, and the liver function recovered well in all cases postoperatively. Conclusion: Local resection by using IOUS and appropriate approach is applicable for the cirrhotic patients with deeply located central sHCC. IOUS plays an important role in avoidance of injuries to the hepatic veins.

Objective Atrophic gastritis, as a pre-cancerous condition of gastric cancer, is developed from non-atrophic gastritis.The aims of this study was to compare the gene expression profiles between atrophic gastritis and non-atrophic gastritis using cDNA microarray and to explore the molecular mechanisms in the development of atrophic gastritis. Methods Endoscopy and biopsy were performed consecutively in 227 patients (143 male, 84 female, age range between 16-72 years, with average age 48.6 years). Diagnosis of non-atrophic gastritis and atrophic gastritis was made according to histological examination, and 120 patients were in non-atrophic group and 107 in atrophic group. The total RNA was extracted from the biopsy specimens of two groups with Trizol reagent. cDNA microarray consisting 8 464 human genes (HGEC-80s kit) was used, and labeled cDNA with fluorescence probes were hybridized to microarray, and then the comparison of gene expression profiles was made between atrophic and non-atrophic gastritis. Results It was identified that in gene expression profiles of atrophic gastritis there were 165 genes showing a greater than 2-fold increase and 460 genes showing a greater than 50% decrease as compared to non-atrophic gastritis. RT-PCR analysis revealed similar results as in DNA microarray. Conclusions There is different expression in many genes between the gastric mucosal cells of non-atrophic and atrophic gastritis mucosa. Many genes expression profiles are involved in atrophic gastritis that would be of help in further understanding the development of atrophic gastritis.

OBJECTIVE: To discuss therapy regimes and pharmaceutical care for the inpatient with intracranial infection after craniotomy. METHODS: Clinical cases were taken as example and therapy regimes was analyzed in order to put out full range and individualized pharmaceutical care for patients with intracranial infection after craniotomy. RESULTS: Reasonable medication was provided by implementing pharmaceutical care. CONCLUSION: It is necessary to deliver pharmacetical care to the inpatient with intracranial infection after craniotomy.

The mitogen-actived protein kinase(MAPK)is one of important ways in eukaryote signal transmission network,and plays the key role in the gene expression regulation and the cytoplasm function activity.In the mammal organism,five kinds of different MAPK signal transduction passage.ERK1/2 signal transduction passage regulates cell growth and differentiation,JNK and p38 MAPK signal transduction passages play the vital role in the stress of inflammation and apoptosis etc.The activation of signal transduction passage is the typical characteristic of RA chronic synovitis,through this way to induct synoviocytes cytoplasm protein phosphorylation,then causes transcription factor and nucleoprotein c-Fos,c-Jun,AP-1 and NF-kappa B and so on phosphorylation,thus promotes cell proliferation and activation.It has the vital significance of the above-mentioned mechanism research to understand deeply and researche new medicine to RA.

Objective: To systematically evaluate the incidence of falls among empty nesters in China, so as to provide the evidence for preventing falls among empty nesters. Methods: Publications pertaining to cross-sectional studies of falls among empty nesters in China were retrieved from international and national databases, including CNKI, Wanfang Data, VIP, Cochrane Library, Web of Science and PubMed from inception to August, 2022. Meta-analysis was performed using the software Stata 15.1, and the combined incidence and 95%CI of falls among empty nesters in China was analyzed. Sensitivity analysis was performed using the leave-one-out method to evaluate the stability of outcome indicators. In addition, the publication bias was evaluated using Funnel plot. Results: Initially, 795 articles were screened in the literature, and 17 articles were finally included, covering 5 468 participants. Meta-analysis revealed that the incidence of falls among empty nesters in China was 23.0% (95%CI: 16.6%-29.5%). Subgroup analysis showed that the incidence of falls among empty nesters published from 2016 to 2022 was higher than that from 2008 to 2015 (20.4% vs. 17.3%; P<0.05). The incidence of falls among empty nesters in western regions was higher than that in eastern and central regions (27.4% vs. 15.9% and 15.0%; P<0.05). The incidence of falls among empty nesters in urban areas was higher than that in rural areas (20.8% vs. 9.9%; P<0.05). Sensitivity analysis showed that the study results were stable, and the funnel plot identified some publication bias. Conclusion The incidence of falls among empty nesters in China was 16.6% to 29.5%, and it is recommended to strengthen the assessment and prevention of risk factors of falls among empty nesters.

Objective To study the assessment of airway inflammation in asthma patients with airway remodeling by high resolution spiral CT(HRCT).Methods Retrospective analysis on 60 cases of chronicity-persistent period asthma patients with induced sputum IL-4 test,airway mucosa biopsy,HRCT scan; while 20 patients only used HRCT scan as control group.To compare the difference of sputum IL-4,HRCT test of airway wall thickness(WT),wall area(WA)and airway wall relative area(WA％)in different degrees of asthma paitents.Results WA,WA％and WT were significantly increased in different degrees of asthma patients when compared to control group(P<0.05),there were significant difference among each group(P<0.05).WA％and WT were positively correlated with induced sputum IL-4.Conclusion HRCT can effectively evaluate the airway inflammation in airway remodeling of asthma patients,which can be used to evaluate the severity of asthma,treatment effect and follow-up observation.

OBJECTIVE:To study the protective effect of tanshinoneⅡA(TSⅡA)sulfonate injection against vancomycin(VAN)-induced renal injury model rats and its mechanism. METHODS:72 rats were randomly divided into blank group,model group, positive control group(amifostine,1 mg/kg)and TSⅡA sulfonate injection low-dose,medium-dose and high-dose groups(15,30, 60 μg/kg),with 12 rats in each group. Except for blank group,those groups were given VAN(200 mg/kg)intravenously via tail vein to induce renal injury rat model;after modeling,each drug group was given relevant medicine intraperitoneally once a day, and blank group and model group were given normal saline intragastrically for consecutive 10 days. The levels of 24 h protein, NAG and KIM-1 in urine were determined,and those of Cys C,Scr and BUN in serum and those of SOD,MDA,GSH-Px and NO in renal homogenate were also determined;the pathological change of renal tissue was observed. RESULTS:Compared with blank group,the levels of Cys C,Scr and BUN in serum,those of 24 h protein,NAG and KIM-1 in urine and those of MDA and NO in renal tissue increased significantly in model group,while the levels of SOD and GSH-Px decreased significantly(P<0.01);the pathological slice indicated that model group suffered from renal injury such as kidney tubules albuminoid degeneration,brush border abscission,renal tubular epithelial cell disintegration and abscission. Compared with model group,the levels of Cys C,Scr and BUN in serum,those of 24 h protein,NAG and KIM-1 in urine and those of MDA and NO in renal tissue decreased signifi-cantly in treatment groups,while the levels of SOD and GSH-Px in renal tissue increased significantly(P<0.05 or P<0.01);path-ological changes of renal tissue were relieved significantly. CONCLUSIONS:TSⅡ A sulfonate injection can effectively relieve VAN-induced renal injury in rats,and its mechanism may be associated with inhibiting the oxidative reaction of rats in vivo.

Objective To investigate the effects of plateletpheresis on blood conservation following cardiopulmonary bypass.Methods 20 patients undergoing open heart surgery were divided randomly into experiment and control groups.All patients underwent routine CPB.Plateletpheresis was performed in experiment group by using COBE SPECTRA.Other blood conservation approaches were similar in two groups.Results The number of platelets collected in experiment group exceeded 20% of total platelets of every patients.In the experiment group,less transfusion was need.The changes of Hb and Hct at every time point were similar in the two groups.Platelet counts,aggregation,and prothrombin time in the experiment group restored to preoperative levels significantly earlier than in the control group.The chest tube drainage at 2,4,8,and 16 h and the total drainage after operation were significantly lower in the experiment group than those in the control group.Conclusion By protecting platelets from destruction by extracorporeal circulation,plateletpheresis is beneficial to the recovery of haemostatic system.It is a useful blood conservation method.

Objective To investigate the effects of H. pylori infection on cyclooxygenase 2 (COX 2) expression in gastric antral mucosa. Methods Antral biopsies were taken from 46 patients with chronic gastritis and H. pylori infection before and after anti H. pylori treatment. COX 2 protein was stained by immunohistochemical method. Gastritis and H. pylori infection status were graded according to the updated. Sydney system. Results Cytoplasmic staining of COX 2 protein could be detected in epithelial cell both before and after eradication of H. pylori . The mean percentage of the cells staining with COX 2 was significantly higher in H. pylori infected mucosa, compared with the mucosa after successful H. pylori eradication [(20.1?13.1)% vs (13.8?5.9)%, P