Objective To investigate the correlation between serum HCV-RNA level and anti-HCV with liver fibrosis indexes in the patients with chronic hepatitis C.Methods The serum samples were collected in 96 cases of chronic hepatitis C.The HCV-RNA level was determined by real time fluorescence quantification PCR and Anti-HCV was detected by ELISA.LN,PCⅢ and CⅣlevels were detected by the electrochemical iuminescence analyzer and HA was examined by radioimmunoassay(RIA).The correla-tion between the HCV-RNA and Anti-HCV levels with the liver fibrosis indexes was analyzed with the statistic software.Results Among these 96 cases of Anti-HCV positive chronic hepatitis C,52 cases (54.2%)were HCV-RNA positive.With increasing of the S/CO value of Anti-HCV,the detection rate of HCV-RNA was increased,which were 9.5%,37.1% and 92.5%.The differ-ences in HA,LN,PCⅢ and CⅣ levels between the high viral load group and the low viral load group had no statistical significance (P >0.05).Conclusion The positive detection rate of HCV-RNA is related with the S/CO value of Anti-HCV,the higher the S/CO value,the higher the positive detection rate of HCV-RNA.But the HCV-RNA level has no correlation with the liver fibrosis in-dexes.

BACKGROUND:Brain-derived neurotrophic factor has been detected in bone and tooth, and its role in development and metabolism of bone and tooth tissue as wel as its clinical application has become a hot spot. OBJECTIVE: To summarize and analyze the effect and mechanism of brain-derived neurotrophic factor in development and metabolism of bone and tooth tissues. METHODS: Papers addressing the effect of brain-derived neurotrophic factor in bone and tooth tissue were retrieved by computer in Wanfang and PubMed databases with the key words of “brain-derived neurotrophic factor, TrkB, p75NTR, signaling, bone, tooth, osteoblasts, osteoclasts” in Chinese and English, respectively. A total of 53 papers were included for review. RESULTS AND CONCLUSION:Brain-derived neurotrophic factor can be detected in various tissuesin vivo, and can regulate cel survival and apoptosis through binding its two receptors. Brain-derived neurotrophic factor in bone and tooth tissue can bind to target cels, induce or inhibit cel proliferation and differentiation, indicating that brain-derived neurotrophic factor is closely linked to bone and tooth tissue, and may play a role in growth and reconstruction of bone and tooth. Its mechanism of action is mainly through binding to TrkB receptor, to activate downstream pathways and affect differentiation and proliferation of mesenchymal stem cels, osteoblasts, chondrocytes, and periodontal ligament cels. Interaction between p75NTR receptor and TrkB receptor may be one of the factors affecting cel differentiation or proliferation.

OBJECTIVE: To determine and compare the contents of tanshinone Ⅱ A in difference dosage form of Guanxin danshen preparations(tablets,capsules,granules and dripping pills).METHODS: The tanshinone Ⅱ A in different Guanxin danshen preparations was extracted by ultrasonic extraction with methanol as solvent,and the content of tanshinone Ⅱ A was determined by HPLC on Agilent Zorbax C8(150 mm?4.6 mm,5 ?m) column with mobile phase consisted of methanol-water(72∶28)under a detection wavelength of 270 nm.RESULTS: The linear ranges for tanshinone Ⅱ A stood at 2.76～138 ?g?mL-1(r=0.999 9).The average recoveries of Guanxin danshen tablets,Guanxin danshen capsules,Guanxin danshen granules and Guanxin danshen dripping pills were 99.29%,100.37%,100.18% and 98.71%,respectively with RSD at 1.78%,2.10%,1.55%,and 1.09%(all n=6),respectively.CONCLUSION:The content of tanshinone Ⅱ A in differed greatly in different Guanxin danshen preparations;therefore it is urgent to establish the quality standard for Guanxin danshen preparations as soon as possible.

Objective To investigate the rehabilitation staffing in community health care center in Guangzhou City. MethodsThe basic personal information, work condition, work load, problems in special staff of rehabilitation for disabled persons were investigated with self-report questionnaires. Results143 staff (92.86%) had accepted higher education, 38 (24.68%) practiced beyond 3 years. The main work was the rehabilitation services for local inhabitants, and the other services have not been carried out widely. The main problems reported were lack of finance, staff and skills training. 83.77% promised that they would provide the rehabilitation services meeting the governmental standard. ConclusionStaff who work for the community health care center have more professional education and experience, compared with years ago. The services need more normalization, and the financial support is also needed.

Objective To investigate the correlation between I405 single nucleotide polymorphism in CETP gene and the therapeutic effect of simvastatin in dyslipidemia patients.Methods Genotype information of I405V in CETP gene was detected by SNaPshot TM primer extension assay in 375 dyslipidemia patients.Blood lipid profiles before and 6 weeks after treatment with simvastatin were recorded to analyze the correlation between the therapeutic effect of simvastatin and I405 polymorphism genostype.Results The frequency of I405 V polymorphism was 38.9%in II genotype,48.5% in IV genotype and 12.5%in VV genotype.After being treated with simvastatin for 6 weeks,the serum levels of TC were significantly decreased by 1.21 mmol/L(P <0.01)and HDL-C level was significantly increased by 0.74 mmol/L(P <0.01).Compared with the IV and VV genotypes of I405V polymorphism,the decrease of TC in II genotype patients were 0.26 mmol/L and 0.16 mmol/L more respectively (P<0.01).Meanwhile,the increase of HDL-C was significantly different among three genotypes(P<0.01).HDL-C in II genotype was increased by 0.85 mmol/L,which was 0.19 mmol/L and 0.15 mmol/L more compared with IV genotype and VV genotype,respectively.Conclusion I405V polymorphism in CETP gene is similar with the lipid-regulating effect of simvastatin.The decrease of blood lipid is more significant in patients with II genotype compared with patients with V allele,suggesting that II genotype is the maker which may predict the therapeutic effect of simvastatin in dyslipidemia patients.However,this conclusion still needs further evaluation.

Objective To investigate the effect of systematic training on the enhancement of knowledge and behavior on tumor-free technology among newly-employed nurses in operation room.Methods Fifteen nurses newly-employed for the operating room of the Affiliated Hospital of Luzhou Medical College were trained with theory teaching,techniques training and nursing rounds. The test on non-tumor knowledge and questionnaire on beliefs and behaviors were used to assess the post-training effects.Result The scores on tumor-free knowledge,beliefs and tumor-free behaviors were all significantly improved compared with pre-training (P<0.01). Conclusion Systematic training of tumor-free technology may improve the mastery of tumor-free knowledge and skills and thus form a good tumor-free concept so that they can keep tumor-free techonology in practice.

To explore the effects of two breathholding and exerting methods on the second labor course of primiparas.Methods Six hundred primiparas were divided according to the admission time into two groups: the control group and observation group. The former took conventional breathholding and exerting method and the latter breathholding and exerting method with breathing techniques. The two groups were compared in terms of outcome of delivery, rate of episiotomy, time for breathholding and exerting and the hemorrhage volume 2h after delivery, perineal laceration and neonate asphyxia.Results The rate of spontaneous delivery in the observation group was higher than that in the control group. The rate of episiotomy, bleeding volume 2h after delivery and perineal laceration were lower. The breathholding time was significantly shorter(P<0.05).Conclusion Breathholding and exerting at the second stage of labor can reduce energetic consumption and improve the outcome of delivery.

Objective: To investigate the influence of social comparison and social support on negative physical self of female from adolescents to young adulthood. Methods: 980 female students aged from 12 to 22 completed NPSS-F (negative physical self scale-fatness), MSPSS (multidimensional scale of perceived social support) and PACS (physical appearance comparison scale). Their body mass index (BMI) was also calculated by weight and height of the subjects. Results: High school students had higher score in Fatness dimension than middle school students (13.5?9.3/11.0?9.0,P

As an important part in hospital quality management,medical metrology management is a technical support and necessary means for medical quality.Four aspects such as reinforcement of organization,rules,basic construction and exertion of function are discussed.It is impsrtant and esstntial to strengthen medical metrology.[Chinese Medical Equipment Journal,2008,29(2):107,109]

Eating disorders is a weight and eating related syndrome with cognitive,emotional and behavioral barriers.Prevention of eating disorders is significant.There are three theoretical models on the prevention of eating disorders:non-specific vulnerability-stressor model,social cognitive model,and critical social perspectives model.It's necessary to combine these three models together for effective prevention.