Objective To investigate the correlation between serum HCV-RNA level and anti-HCV with liver fibrosis indexes in the patients with chronic hepatitis C.Methods The serum samples were collected in 96 cases of chronic hepatitis C.The HCV-RNA level was determined by real time fluorescence quantification PCR and Anti-HCV was detected by ELISA.LN,PCⅢ and CⅣlevels were detected by the electrochemical iuminescence analyzer and HA was examined by radioimmunoassay(RIA).The correla-tion between the HCV-RNA and Anti-HCV levels with the liver fibrosis indexes was analyzed with the statistic software.Results Among these 96 cases of Anti-HCV positive chronic hepatitis C,52 cases (54.2%)were HCV-RNA positive.With increasing of the S/CO value of Anti-HCV,the detection rate of HCV-RNA was increased,which were 9.5%,37.1% and 92.5%.The differ-ences in HA,LN,PCⅢ and CⅣ levels between the high viral load group and the low viral load group had no statistical significance (P >0.05).Conclusion The positive detection rate of HCV-RNA is related with the S/CO value of Anti-HCV,the higher the S/CO value,the higher the positive detection rate of HCV-RNA.But the HCV-RNA level has no correlation with the liver fibrosis in-dexes.

BACKGROUND:Brain-derived neurotrophic factor has been detected in bone and tooth, and its role in development and metabolism of bone and tooth tissue as wel as its clinical application has become a hot spot. OBJECTIVE: To summarize and analyze the effect and mechanism of brain-derived neurotrophic factor in development and metabolism of bone and tooth tissues. METHODS: Papers addressing the effect of brain-derived neurotrophic factor in bone and tooth tissue were retrieved by computer in Wanfang and PubMed databases with the key words of “brain-derived neurotrophic factor, TrkB, p75NTR, signaling, bone, tooth, osteoblasts, osteoclasts” in Chinese and English, respectively. A total of 53 papers were included for review. RESULTS AND CONCLUSION:Brain-derived neurotrophic factor can be detected in various tissuesin vivo, and can regulate cel survival and apoptosis through binding its two receptors. Brain-derived neurotrophic factor in bone and tooth tissue can bind to target cels, induce or inhibit cel proliferation and differentiation, indicating that brain-derived neurotrophic factor is closely linked to bone and tooth tissue, and may play a role in growth and reconstruction of bone and tooth. Its mechanism of action is mainly through binding to TrkB receptor, to activate downstream pathways and affect differentiation and proliferation of mesenchymal stem cels, osteoblasts, chondrocytes, and periodontal ligament cels. Interaction between p75NTR receptor and TrkB receptor may be one of the factors affecting cel differentiation or proliferation.

OBJECTIVE: To determine and compare the contents of tanshinone Ⅱ A in difference dosage form of Guanxin danshen preparations(tablets,capsules,granules and dripping pills).METHODS: The tanshinone Ⅱ A in different Guanxin danshen preparations was extracted by ultrasonic extraction with methanol as solvent,and the content of tanshinone Ⅱ A was determined by HPLC on Agilent Zorbax C8(150 mm?4.6 mm,5 ?m) column with mobile phase consisted of methanol-water(72∶28)under a detection wavelength of 270 nm.RESULTS: The linear ranges for tanshinone Ⅱ A stood at 2.76～138 ?g?mL-1(r=0.999 9).The average recoveries of Guanxin danshen tablets,Guanxin danshen capsules,Guanxin danshen granules and Guanxin danshen dripping pills were 99.29%,100.37%,100.18% and 98.71%,respectively with RSD at 1.78%,2.10%,1.55%,and 1.09%(all n=6),respectively.CONCLUSION:The content of tanshinone Ⅱ A in differed greatly in different Guanxin danshen preparations;therefore it is urgent to establish the quality standard for Guanxin danshen preparations as soon as possible.

Objective To investigate the rehabilitation staffing in community health care center in Guangzhou City. MethodsThe basic personal information, work condition, work load, problems in special staff of rehabilitation for disabled persons were investigated with self-report questionnaires. Results143 staff (92.86%) had accepted higher education, 38 (24.68%) practiced beyond 3 years. The main work was the rehabilitation services for local inhabitants, and the other services have not been carried out widely. The main problems reported were lack of finance, staff and skills training. 83.77% promised that they would provide the rehabilitation services meeting the governmental standard. ConclusionStaff who work for the community health care center have more professional education and experience, compared with years ago. The services need more normalization, and the financial support is also needed.

BACKGROUND: In the researches of Ginkgo Biloba Extract(GBE) in the treatment of cerebra ischemia, because of the application of generally anaesthesia medication that may induce the alteration of cerebral temperature, the accuracy of the results may be affected.OBJECTIVE: To investigate the impact of domestic GBE on antioxidase and lipid peroxide of cerebral ischemic reperfusion tissue as well as the water content of ischemic brain tissue under normothermia.DESIGN: A randomised controlled trial.SETTING and MATERIALS: Study was conducted in the Tongji Medical University of Huazhong Science and Technology University. A total of 24 Wistar rats with a mass from 250 g to 300 g were randomly allocated into sham-operation group ( n = 8 ), cerebral ischemia control group ( n = 8) and cerebral ischemia GBE treatment group( n = 8) . The animal model of normothermia rat with left middle cerebral artery ischemia for 2 hours and reperfusion for 2 hours was prepared with the reference of Nagasawa method in the animals of control group and treatment group for contrast study.INTERVENTIONS: The cerebral temperature of the rats was reflected by the temperature of the temporal muscle. The temperature-measuring probe was embedded into the deep part of the left temporal muscle closed to osseous ectoblast. The temperature was continuously monitored by semiconductor oxide temperature sensor. The temperature of the head was heated with 60 W filament lamp and adjusted by automatic double-controlling craniocerebral cooling instrument to maintain the cerebral temperature at normothermia level of 36.5 ℃ - 37 ℃. The normothermia cerebral ischemic reperfusion injury rat model was established according to the design. GBE injection was injected respectively into abdominal cavity in the rats of cerebral ischemia GBE treatment group at the following time point: 12 hours, 8 hours and 4 hours before operation, immediately after cerebral ischemia and immediately after reperfusion, with 3 mL each time and 5 times in total. Same times and dose of normal saline was injected into the abdominal cavity in the rats of both control group and sham-operation groups.MAIN OUTCOME MEASURES: Superoxide dismutase (SOD), glutathione peroxidase(GSH-Px), reduced glutathione(GSH), malondialdehyde(MDA)and water contents in the cerebral ischemic tissue.RESULTS: The levels of SOD, GSH-Px and GSH in cerebral ischemia control group were(73.35 ± 12. 86) NU/mg, (167.37 ±54.34) μkat/g and (196. 84 ± 22.75) μg/g respectively, which significantly lower than that (96. 02± 16. 83) NU/mg, (338.57±84.02) μkat/g and(337.51± 34. 89) μg/g of sham-operation group( P ＜ 0. 01 or P ＜ 0.05) . The SOD, GSH-Px and GSH levels of cerebral isehemia GBE treatment group were (87.24± 15.03) NU/mg, (316. 56 ±93.52) μkat/g and(263.16±28.54) μg/g, which significantly higher than that of cerebral ischemia control group(P ＜ 0.01 or P ＜ 0.05) .The MDA level of cerebra ischemia control group was (308.34 ± 26.81 ) nmol/g, which significantly higher than that(101.46 ± 10.97) nmol/g of sham-operation group( P ＜ 0.01 ) .The MDA level of cerebral ischemia GBE treatment group was(125.86± 13.90) nmol/g, which was significantly lower than that of sham-operation group ( P ＜ 0.01 ) . The water content of cerebral ischemia control group was(80. 45 ± 0.44)%, which was significantly higher than that (78.20 ± 0. 25 ) % of sham-operation group ( P ＜ 0.01 ) . The water content of cerebral ischemia GBE treatment group was(79.63 ± 0.46) %, which was significantly lower than that of cerebral ischemia control group( P ＜ 0.05).CONCLUSION: Domestic GBE can inhibit the excessive production of free radicals and the lipid peroxidation during cerebral ischemia and reduce cerebral oedema and the destruction of blood-brain barrier to protect cerebral ischemic tissues under cerebral normothermia.

Eating disorders is a weight and eating related syndrome with cognitive,emotional and behavioral barriers.Prevention of eating disorders is significant.There are three theoretical models on the prevention of eating disorders:non-specific vulnerability-stressor model,social cognitive model,and critical social perspectives model.It's necessary to combine these three models together for effective prevention.

Objective To analyze the chara cteristic finding of metachromatic leukodystrophy (MLD) on MR images. Methods Eleven patients with biochemicall y proved MLD were studied retrospectively for the extent of white matter abnormalities and the in volvement of specific structures (such as corpus callosum, white matter tract, a nd thalami). Result All 11 cases in the periventricular white m atter and 10 cases in centrum semiovale showed a symmetric confluent high intens ity on T_2-weighted images. Involvement of subcortical U-fibers was present i n 4 late-stage cases. Other sites of involvement were the genu (n=8) and s plenium (n=9) of the cor pus callosum (both n=8), the posterior limbs of internal capsule (n=7), external capsule (n=4), the descending pyramidal tracts (pons in 1, midbrain in 3), and the cerebellar white matter (n=1). A tigroid pattern was found in the centru m se miovale in 8 cases. Nine cases appeared low signal intensity on T_2-weighted i mag es in the thalami; There were 5 cases with mild ventriculomegaly and 1 case with diffuse brain atrophy. Conclusion The typical MR feature of M LD is a bilatera l and symmetrical high signal intensity on T_2-weighted images in periventricu lar and centrum semiovale white matter with initial sparing of the subcortical U -fi ber, and both the genu and splenium of corpus callosum involvement. Besides, the tigroid pattern in the centrum semiovale and involvement of the internal capsul e, external capsule, and corticospinal tract are frequent additional features.

Objective To study the levels of soluble tumor necrosis factor receptor (sTNFR)、 tumor necrosis factor alpha(TNF? ) in systemic lupus erythematosus(SLE) patients and its clinical significance. Methods Double antibody sandwich ELISA was used to determine soluble tumor necrosis factor receptor typeⅠ (sTNFRⅠ )、 soluble tumor necrosis factor receptor typeⅡ (sTNFRⅡ ) and TNF? levels in patient serum and peripheral blood mononuclear cell(PBMC) culture supernatants. Other immunological tests were carried out simultaneously. Results① The serum sTNFRⅠ、 sTNFRⅡ、 TNF? sTNFRⅠ /TNF? and sTNFRⅡ /TNF? ratios were significantly higher in SLE patients than those in normal control group(P

Objective: To investigate the influence of social comparison and social support on negative physical self of female from adolescents to young adulthood. Methods: 980 female students aged from 12 to 22 completed NPSS-F (negative physical self scale-fatness), MSPSS (multidimensional scale of perceived social support) and PACS (physical appearance comparison scale). Their body mass index (BMI) was also calculated by weight and height of the subjects. Results: High school students had higher score in Fatness dimension than middle school students (13.5?9.3/11.0?9.0,P

As an important part in hospital quality management,medical metrology management is a technical support and necessary means for medical quality.Four aspects such as reinforcement of organization,rules,basic construction and exertion of function are discussed.It is impsrtant and esstntial to strengthen medical metrology.[Chinese Medical Equipment Journal,2008,29(2):107,109]