Objective:To evaluate the cytotoxicity of sodium hypochlorite(NaOCl) and chloramine-T at a same available chlorine concentration. Methods: L929 fibroblasts were used to test the cytotoxicity of NaOCl and chloramine-T at a same available chlorine concentration (0.500 0%, 0.250 0%,0.200 0%, 0.150 0%, 0.125 0%, 0.062 5%). Cell viability after 2 h, 30 min or 10 min exposure were measured by MTT assay. Results: Except the 0.500 0%-2 h group and 0.062 5%-30 min group, cell viability of NaOCl groups were higher than chloramine-T group(P<0.05). Conclusion: NaOCl solution has less cytotoxicity than chloramine-T at a same available chlorine concentration lower than 0.250 0%.

For the children,viral encephalitis is a common infectious disease of central nervous system,which is characterized by high incidences,unfavourable prognosis and high mortality rate.The Japanese encephalitis virus,herpes simplex virus,enteroviruses,EB virus,cytomegalovirus are the common pathogens.There are some emerging virus also cause encephalitis,such as west nile virus,borna disease virus,banna virus,nipah virus and so on.It is of significance for the doctors and scientific research personnels to study the pathogens of viral encephalitis.

Objective To analyze themonitoring results of environmental hygiene inthe Blood Purification-Center and explore the management. Methods Hospitalinfection control personnel carried on the environmental hygiene monitoring in the blood purification center from 2010 January 2010 to December 2014. A total of 656 sampleswere examined , with monitoring covering airindex , surface of objects , hands of medical staffs and dialysis water. The infections from temporarydouble lumen catheteration were analyzed during the period. Results Of the 656 examinedsamples, 613 were qualified, with the overall pass rate of 96.4%. All the monitoring samples basi-cally achieved the qualified standard, with zero detection rate of the pathogen. The temporarydouble lumen catheterinfection rate was decreased from 8.6% to 3.4% during the period andthe difference was statistically sig-nificant between 2014 and 2010 (χ2 = 7.73, P < 0.01). Conclusion Strengthening the environmental hygiene monitoring may help increase the dialysis treatment and nursing work , decrease the infection rate of temporary dou-ble lumen catheter in hemodialysis patients and improve their quality of life.

OBJECTIVE:To improve the quality standard for Ganmao shangfengke tea. METHODS:TLC was used to identify the Saposhnikovia divaricata and Glycyrrhizae Radix et Rhizoma in the preparation. HPLC was used to determine the content of magnolol and honokiol:the column was Phenomenex Luna C18 with mobile phase of methanol-water(77:23,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 294 nm,column temperature was room temperature,and the injection volume 5 μl. RE-SULTS:The TLC spots of S. divaricata and Glycyrrhizae Radix et Rhizoma were clear and well separated. The linear range was 3.13-100.2 μg(r=0.9999) for magnolol and 3.05-97.6 μg(r=0.9999) for honokiol;RSDs of precision,stability and accuracy tests were lower than 2%;recoveries were 98.2%-99.5%(RSD=0.5%,n=6)and 98.6%-99.6%(RSD=0.4%,n=6),respective-ly. CONCLUSIONS:The improved standard can be used for the quality control of Ganmao shangfengke tea.

Objective To study the expression of KAI1mRNA in cervical carcinoma and its clinical significance.Methods The transcription-polymerase chain reaction(RT-PCR) method was applied to detect the expression of KAI1mRNA in 53 fresh freezing cervical tissues.The findings were compared with the clinicopathological data.Results The expression of KAI1mRNA was (0.309?0.093) in normal cervix,(1.608?0.289) in cervical intraepithelial neoplasia(CIN),(0.948?0.910) in cervical carcinoma respectively.The relative densitometric value of KAI1mRNA decreased in late stage of cervical cancer in local cervical cancer with lymphatic metastasis(P

Objective To study the diagnostic value of TCD and MRI in transient ischemic attack (TIA) of internal carotid artery system.Methods The clinic data was analyzed to contrast the relation of the clinic manifestation and the TCD and MRI results of 66 patients suffered from TIA of the internal carotid artery system.then chi-square test was adopted.Results In TIA interval, extensive hemodynamics changes were occurred in the intracranial arteries among 60 patients(90 9%), and 22 patients(33 3) presented different degrees of stricture on the corresponding symptom side, MRI test showed lacunar infarct or small infarct foci among 50 patients(75 8%), and ischemic leukoenporosis among 35 patients(53 5%). But the foci took a two-side distribution and were relatively symmetrical. No significant correlation existed between the symptom and the foci,and the positive rate of TCD did not show remarkable difference between the symptoms and the foci, and the positive ratio of TCD did not show remarkable difference whether the MRI infarct foci existed or not. Meanwhile, there were also no notable distinction between the stricture change of arteries in the corresponding symptom side with the infarct foci and that of arteries in the corresponding symptom side without the infarct foci.Conclusions TCD can find the change of arteries related in TIA of the internal carotid artery system, and can be used as routine and primary screening. While MRI may bear no special clinic significance to TIA of the internal carotid artery system, but it has localization meaning to CWS, a clinical subtype of TIA.

OBJECTIVE:To observe the effect of Ginkgo biloba extract injection on the urinary microalbumin excretion rate in patients with early diabetic nephropathy(DN).METHODS:A total of 84 patients with early DN were randomly divided into two groups:the control group was treated with routine therapy while the treatment group was treated with routine therapy plus Ginkgo biloba extract injection(at a dose of 10mL)q.d for 3 consecutive weeks.The urinary microalbumin excretion rate and the related clinical and biochemical indicators were compared between the two groups before and after treatment.RESULTS:As compared with the control group,urinary microalbumin excretion rate was significantly lowered in the treatment group(P

Screening of CHD of infants (11 178) born from May 1st 2012 to April 30th 2013.92 infants were screening positive,68 were diagnosed.The detectable rate of CHD was 6.08‰,while boys were 3.31‰,girls were 2.77‰.68 CHD patients,the top three were VSD(2.14‰),PDA(1.34‰) and ASD (0.89‰).In these infants,detectable cardiac souffle were 61,abnormal pulse oximetry were 40.The detectable rate of CHD among infants was identical compare the average level of whole country,but the distributional characteristics was disparity.The screening of CHD was feasible,and cardiac souffle and pulse oximetry were recommended.

Objective To explore the causes of respiratory tract obstruction during resuscitation of general anesthesia and nursing strategy.Method The clinical data of 13 patients with respiratory tract obstruction during resuscitation of general anesthesia after extubation were reviewed.Results The causes of respiratory tract obstruction included glossocoma,laryngismus,bronchial spasm,airway blockage with secretions and tracheal compression.The respiratory tract obstruction was cured by way of pertinent treatment.Conclusion The critical measures for the treatment of respiratory tract obstruction include careful observation and prompt detection of abnormal symptoms of hypoxia and respiratory index,and effective treatment.

Objective:To explore the impact of cytochrome P450 oxidase (CYP2C19) *2 genotype on major adverse cardiovascular events (MACE) after percutaneous coronary intervention (PCI) and impact of MACE on blood stasis indexes .Methods :From Jan 2010 to Jan 2011 ,a total of 355 patients with blood stasis syndrome undergoing PCI were enrolled .All patients took aspirin and clopidogrel conventionally before PCI ,then maintained for one year at least after PCI .According to MACE or not ,patients were divided into MACE group (n=64) and no MACE group (n=291) .Turbidimetry method was used to measure platelet aggregation rate before and one week after PCI ,and polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP ) was used to test CYP2C19 * 2 gene polymorphism .Results:(1) Compared with no MACE group ,there were significant rise in maximum platelet aggregation rate [MPA ,(36.38 ± 14.57)% vs .(44.87 ± 11.63)% ] and residue platelet aggregation rate [RPA , (25.76 ± 12.69)% vs .(34.45 ± 15.58)% ] , P< 0.01 both ;in percentage of CYP2C19*2 gene mutation (GA+AA) (36.43% vs .75.0% ,χ2 =27.832 ,P<0.01) in MACE group .(2) Multi-factor Logistic regression analysis in-dicated that >65 years ,CYP2C19*2 gene mutation (GA+AA) ,renal dysfunction (creatinine>1.5mg/dl) and re-duced left ventricular ejection fraction (LVEF< 50% ) were independent risk factors for MACE after PCI (OR=2.164~5.947 , P<0.05 or <0.01);(3) Binary linear correlation analysis indicated that CYP 2C19*2 gene muta-tion was positively correlated with RPA after PCI ( r=0.437 , P<0.001) .Conclusion:CYP2C19*2 gene mutation affects antiplatelet activity of clopidogrel ,so it′s helps to recognize MACE risk in patients with coronary heart dis-ease and blood stasis after PCI .