Six compouuds were isolated for the fitst time from Spiraca mongolica Maxim. (Rosaceae by means of chemical and spectral analyslsand comparison with data of literature or authentie samples, 5 of them were identified as ?-sitosterol(Ⅰ), betulin(Ⅱ), betulinic acid (Ⅲ),betuliuic acid 3, 5-dihydroxyl-cinuamate(Ⅳ) and daucosterol(Ⅵ).

The activation of MAPKs signal transduction parthways, is a typical feature of chronic synovitis in rheumatoid arthritis. The phosphorylation of synoviocytes cytoplasm proteins induced transcription factor and nucleus proteins phosphorylation such as c-fos, c-jun, AP-1 and NF-?B via MAPKs signal transduction, which promotes synoviocytes proliferation and activation. These findings may be beneficial for the elucidation of the pathogenesis and the development of new drugs for rheumatoid arthritis.

Objective To explore the mediating effect and moderating effect of professional psycho?logical help?seeking intention and self?efficacy between mental symptoms and professional psychological help?seeking behavior in Chinese adults. Methods Totally 618 adult samples were selected on the internet and completed measures of help?seeking questionnaire,ISCI,SCL?90 and GSES. Results Females and middle a?dulthood subjects got higher ISCI and GSES scores than males and early adulthood subjects ((82.7±6.7) vs (77.8±7.8),(83.9±6.2) vs (76.8±7.3);all P<0.01). SCL?90 was significantly and positively correlated with psychological help?seeking behavior( r=0.162, P<0.01).ISCI was found to mediate the association be?tween SCL?90 and psychological help?seeking behavior(effect value=-0.004,95%CI=-0.008?-0.002). GSES significantly moderated the mediating effect of ISCI( B=0.006, P<0.05). Conclusion ISCI plays a mediating role between mental symptoms and professional psychological help?seeking behavior, which was moderated by GSES in Chinese adults.

Objective:To study the expression and significance of apoptosis-related gene tfar19 in normal oral mucosa, oral leukoplakia and oral squamous cell carcinoma.Methods:The expression of TFAR19 was observed in 17 cases of oral normal mucosa, 60 of oral leukoplakia and 30 of oral squamous cell carcinoma by immunohistochemical stain.Results:①Positive expression of TFAR19 was observed in 88.2% of the cases of oral normal mucosa, 63.3% of oral leukoplakia, and 30% of oral squamous cell carcinoma. There was statistical difference between each two groups (P

Lymphoma is a malignancy of mature lymphocytes. Signalling through the B cell receptor ( BCR ) is central to the development and maintenance of B cells. In light of the numer-ous proliferative and survival pathways activated downstream of the BCR, it comes as no surprise that malignant B cells would co-opt this receptor to promote their own growth and survival. Compounds that inhibit various components of this pathway, in-cluding spleen tyrosine kinase(Syk), Bruton’s tyrosine kinase (Btk), and phosphoinositol-3 kinase(PI3K), have been devel-oped. In this paper,the B-cell receptor signaling and its targeted inhibitors of lymphoid malignancies are reviewed.

Objective To analyse the detection rates and antibiotic resistance of extended-spectrum β-lactamases (ESBLs) producing Klebsiella pneumonia and Escherichia coli in Intensive Care Unit (ICU) and to guide the clinical administration of treatment Methods Klebsiella pneumonia and Escherichia coli collected from clinical samples from January 2008 to December 2010 were tested by Phenotypic Confirmatory Test and confirmed by the method advised by NCCLs and drug-sensitivity was tested with K-B. Results Among the isolated 90 samples,49 strains were considered ESBLs-producing bacteria (54.4%) .with 52. 5% (31/59)of Klebsiella pneumonia and 58. 1% (18/31) of Escherichia coli respectively; with the specimens of respiratory system having the highest rate of 75. 5% (37/49). ESBLs producing bacteria were highly resistant to penicillins and cephalosporins, multi drug resistant to aminoglycosides and quinolones; low to piperacillin/tazobactam,cefoperazone/sulbactam,cefoxitin and amikacin; and all sensitive to imipenem. When compared to non-ESBLs producing strains, the rates of antibiotic resistance of the producing ESBLs strains were significantly higher. Conclusion The test results showed that the isolation rates of ESBLs-producing Klebsiella pneumoniae and Escherichia coli in ICU were high,which had high resistance to most antimicrobial agents,and the resistance was multiple. Imipenem could be the best choice to control the infection due to ESBLs-producing organisms. Timely detection of ESBLs producing bacteria and drug resistance is essential to guide clinical antibiotic using in ICU.

Objective To investigate the chemical constituents of Dimocarpus longan seeds in Sapindaceae.Methods The chemical constituents were isolated from the ethanol extract of D.longan seeds by silica gel column chromatography.Their structures were identified on the basis of physical and chemical properties and spectral analysis.Results One compound was isolated and identified as 2-methyl-1,10-undecanediol,named longandiol(1).Conclusion Compound 1 is a new compound.

BACKGROUND: Fibrin is a kind of high polymer materials with biodegradation and good histocompatibility, and is a vector that can promote celland exogenous growth factor release. Fibrin stabilizing factor XIII has been verified to contribute to the migration of undifferentiated mesenchymal stem cels in gel scaffold with high crosslinking, and promote cellproliferation and differentiation. OBJECTIVE:To observe rat mesenchymal stem cellbehavior in a fibrin gel. METHODS:The rat fetal limbs cels was separated under the aseptic condition. The passage 3 cels were seeded in 0, 5, 10 and 20 g/L fibrin gel. cellmorphology was observed by inverted phase microscope and laser scanning confocal microscopy. Alkaline phosphatase activity and calcium deposition were measured respectively using a microplate reader and von Kossa staining. RESULTS AND CONCLUSION: 5 g/L fibrin gel contributed to cellmorphological changes, and 20 g/L fibrin gel contributed to osteogenic differentiation. Compared with the control group, alkaline phosphatase activity was higher in the formulations containing a 20 g/L fibrinogen concentration. Smal mineralization nodules were observed at 21 and 28 days in a formulation containing both 10 and 20 g/L fibrinogen concentration, but no mineralization was detected in the control group. These results indicate that morphology and osteogenic differentiation of rat mesenchymal stem cels depended on the fibrinogen concentration, suggesting that fibrin gel is conducive to osteogenic differentiation of mesenchymal stem cels.

Objective The article was to promote the rational use of antibiotics by comparative analysis on antimicrobial us -age before and after the special rectification on clinical application of antibacterial drugs in our hospital . Methods Medical record data of 132 inpatients before the rectification and 167 inpatients after the rectification were randomly collected for the rational compara-tive analysis on microorganism submission rate for treatment , usage of antibiotics and medication for typeⅠandⅡincisions . Results The microorganism submission rate was much higher , especially the special grade antibiotics (81.5 %vs 32.1%).There was also a remarkable decrease in the percentage of antibiotics use , the strength of utilization and the average drug cost .The amount of antibiotic prophylaxis without indication and the length of medication for typeⅠincisions showed a remarkable decrease (P<0.05).Remarkable decrease was also observed in the irrational usage rate of antibiotic prophylaxis , dosage, medication time and drug replacement for typeⅠand Ⅱincisions (P<0.05). Conclusion The special rectification measures are effective and practical , which can promote the rational use of antibiotics .

Objective To investigate the value of pathological examination in the diag-nosis of lethal cases due to acute poisoning.Methods The macroscopic and microscopic find-ings in 28 autopsy cases (10 cases of toxic gas,7 cases of pesticide,6 cases of drug,3 cases of alcohol and 2 cases of chinese herbal medicine) died of acute poisoning during the period from October,2001 to June,2012 were retrospectively reviewed.Results Gross Changes were found on gross examination in 22 of the 28 cases studied.Of them,7 cases died of acute carbon mono-xide poisoning had showed cherry red of Shiban,muscle,visceral organs and blood.In addition,11 and 8 cases had been found gastric mucosa hemorrhage and epicardium petechial hemorrhage respectively.Histologically,myocardial interstitial hemorrhage was observed in 9 cases,pulmonary hemorrhage in 18 cases,bronchospasm in 12 cases,cerebral hemorrhage in 4 cases,hepato-cyte necrosis in 11 cases,renal tubular necrosis in 7 cases and gastric mucosa hemorrhage in 11 cases.In 6 cases of oral pesticides poisoning,besides they had all gastric mucosa hemorrhage,the change of character of gastric contents was found in 3 cases and pesticide odor from stomach in 3 cases.In addition,acute inflammatory and necrosis of gastric wall in 6 cases of oral pesticides poisoning were observed under the light microscope.Drug addicts often suffered from chronic lesions in multiple organs,such as chronic liver disease,fatty infiltration of the myocardium with myocardial atrophy,interstitial lung disease and pulmonary foreign body granuloma.Conclusions Pathology examination is helpful in the diagnosis of some lethal cases due to acute ooisoning,and it can provide pathological basis to study on treatment of acute poisoning.