Objective To explore the diagnostic effect of serum level of cystatin C (CysC) on the renal function after neonatal asphyxia by detection of serum level of CysC, blood urea nitrogen (BUN) and serum creatinine (SCr) and calculation of glomerular filtration rate (GFR) in neonatal asphyxia. Methods The clinical data of 86 neonates with asphyxia (46 cases in mild asphyxia group,40 cases in severe asphyxia group) and 30 neonates without asphyxia (control group) were collected and the serum level of CysC, BUN and SCr were detected at 24 h to 72 h after birth. Results Serum levels of CysC, BUN and SCr were (1.97 ±0.33) mg/L, (4.97 ±2.15) mmol/L, (90.41 ±24.32) μmol/L in mild asphyxia group, (2.65 ±0.41) mg/L, (10.88 ± 3.31) mmol/L, (125.82 ± 45.44) μ mol/L in severe asphyxia group and (1.24 ± 0.35)mg/L, (4.25 ± 2.04) mmol/L, (58.41 ± 19.22) μmol/L in control group, respectively. The differences were significant among three groups and those values in mild and severe asphyxia groups were higher than those in control group. The sensitivity of CysC level to evaluate renal function in mild asphyxia group was better than BUN and SCr level (P＜ 0.05). In neonata] asphyxia, the serum level of CysC had negative correlation with GFR (P ＜ 0.01). Conclusions Serum level of CysC can be adopted to evaluate the renal function after neonatal asphyxia, which is better than BUN and SCr. With a higer level of CysC, the renal function injury may be worse.

BACKGROUND:In the treatment of early caries, fluoride can be used for remineralization of white spot lesions. Varnish XT (durable fluoride-releasing coating) and ICON penetration resins are two new materials. Varnish XT as a new type of resin reinforced glass ionomer can be selected as mineralized material. ICON penetration resin is a high-permeability resin with good liquidity that can infiltrate by capilary action into the pores created by enamel demineralization. Low-viscosity resin is used to replace the lost hard tissue due to demineralization and occupy the micropores, so as to fil the region of enamel demineralization and prevent further development of lesions. OBJECTIVE:To study the effects of two minimaly invasive surgical treatment materials, Varnish XT and ICON penetrating resin, on the microhardness of enamel caries white spot. METHODS:Totaly 100 incisors of cows were selected, embedded with ethoxyline resin and polished. The lip side facing down served as the observation side. An area of at least 6 mm×10 mm on the enamel face was exposed, and there were five regions from incisal to dental cervix, A, B, C, D, E. After demineralization liquid for artificial caries, no treatment was adopted in region A, treatment with Varnish XT was for region B, treatment with ICON penetrating resin for region C, treatment with fluoride for region D, and region E was sealed with antacid nail. Surface micro-hardness was detected. RESULTS AND CONCLUSION: After demineralization, surface micro-hardness of regions A, B, C, D decreases remarkably as compared with region E (P < 0.05). Surface micro-hardness of regions B, C, D was higher than that of region A, and ranged as folows: C > B and D for surface micro-hardness with statistical significance (P < 0.05). There was no statistical significance of surface micro-hardness between regions B and D (P > 0.05). ICON resin infiltration, Varnish XT and fluoride have obvious improvement effects on surface micro-hardness, and ICON resin infiltration is superior to Varnish XT and fluoride.

Objective To comprehend the status of Helicobacter pylori (Hp) infection in type 2 diabetes mellitus (T2DM) patients, and study the relationship between Hp and T2DM. Methods Seven hundred and sixty-eight in-hospital T2DM patients (T2DM group, including new diagnosed 194 patients and re-diagnosed 574 patients) and 172 sex, age matched health individuals(control group ) were involved in this study. 14C-urea breath test (14C-UBT) was proceeded in both groups to determine Hp infection, and infection rates were calculated. Results Total Hp infection rate in T2DM group was significantly higher than that in control group [56.0%(430/768) vs 37.8%(65/172) ,P< 0.01], delamination analysis according to sex also showed significant differences between T2DM group and control group(P < 0.01). The rate of Hp infection in new diagnosed patients and re-diagnosed patients, female patients and male patients had no significant difference (P > 0.05 ). The rate of Hp infection in different age and sex had no significant difference (P >0.05 ). Conclusions The rate of Hp infection in T2DM patients is higher than that in non-T2DM patients.Hp infection has relationship with T2DM, and has no relationship with age, sex and course of disease.

Objective To explore the value of quantitive tissue velocity imaging(QTVI) in assessing the systolic myocardial function of adriamycin cardiomyopathy.Methods Conventional echocardiography and QTVI were performed in 7 rabbit models with adriamycin cardiomyopathy and 5 health rabbits as control.The basic segments of interventricular septum of apical 4-chamber view and left ventricular post wall of parastenal left ventricular long axis view were sampled to measure maximal velocity(VIVS,VLVPW),displacement(DIVS,DLVPW),strain rate(SRIVS) and strain(?IVS) in systole.Pre-eject period(PEP) and ratio of pre-eject period and eject time(PEP/ET) were measured with electrocardiography and Doppler spectrums of mitrial and aortic orifices.Results Compared with control,VIVS,DIVS,SRIVS,?IVS and VLVPW,DLVPW decreased(P0.05).Conclusions The indices of QTVI are more sensitive to evaluate systolic function changes than EF,and QTVI may be an optimal method to early diagnose and access of adriamycin cardiomyopathy.

Objective To investigate the effect of berberine on growth and apoptosis of human cervical cancer cell line HeLa and its possible mechanism.Methods The effect of berberine on growth of HeLa cells was studied by MTT assay.Apoptosis of HeLa cells exposed to berberine was observed by flow cytometry and DNA gel electrophoresis.The expression of Bax and Bcl-2 was studied by Western blotting analysis.Results Berberine markedly inhibited the proliferation of HeLa cells in a time-and dose-dependent manner.After incubation of HeLa cells with 20 and 40 mg/L berberine for 48 h,DNA Ladder can be observed.A typical "sub-G1 peak" was checked by flow cytometry.There was a very low rate of natural apoptosis(1.9?0.6)%,while in 5 mg/L berberine group,the apoptosis rate was(2.3?0.8)%.After exposing HeLa cells for 48 h to 20 and 40 mg/L berberine,the apoptosis rate reached(16.7?2.8)%(P

Objective To explore the clinical characteristics of Pierre Robin syndrome and the experience of its diagnosis and therapy, and provide the accurate diagnosis and treatment of Pierre Robin syndrome .Methods Seventeen cases of Pierre Robin syn-drome from September 2008 to August 2013 were enrolled in this study .The clinical data were analyzed retrospectively to explore the clinical characteristics and treatment of Pierre Robin syndrome .Results Seventeen cases of Pierre Robin syndrome had typical clini-cal features, including micrognathia, cleft palate or high palatine arches, glossoptosis, and feeding difficulties.The babies were given corresponding treatment according to their clinical grading. Among those babies, three cases died , including one dying of severe pneu-monia in hospital, and the other two dying of severe pneumonia and malnutrition after giving up treatment .Among 14 survivors, 12 ba-bies were followed up , including 5 babies who achieved optimal growth and development when they were one year old , and the other 7 babies who had feeding difficulties in varying degrees after discharge , and lagged behind normal children in the growth and develop-ment.Conclusions Early diagnosis, accurate classification, and individualized treatment plan for Pierre Robin syndrome might im-prove the prognosis of children with this type of disease .

Objective To evaluate the efficacy of using dual laser system in off clamp nephronsparing surgery.Methods We used dual laser system in the patients who underwent off-clamp NSS between Jan 2016 and Apr 2016 in our institution.There were 16 males and 8 females with average age of 49 years.Mean tumor size was 1.9 cm (range 0.8-3.2 cm).The mean R.E.N.A.L.score was 3.8 (range 3-6).The mean Zhongshan score was 3.5(range 3-5).The mean ZⅡ(zero ischemia index)was 1.6(range 1-4).The Evolve Dual (980 nm/1 470 nm) laser system set at 60 W in continuous mode was used.Results Offclamp NSS was successfully performed in 22 patients except for renal artery occlusion in 2 case.Mean operative time was 74 min(range 50-100 min);The average estimated blood loss was 52.9 ml(range 10-200 ml);Mean postoperative drainage was 65.4 ml(range 20-150ml);Mean postoperative hospital stay was 5.2 days (range:4-7days).No severe post-operative complication was found.The mean pre-and postoperative serum creatinine levels were 76.2 μmol/L(range:48-112μ mol/L) and 81.5μ mol/L(range:54-122 μ mol/L) with no significant difference(P ＞0.05).The postoperative pathology indicated that 20 cases of clear cell carcinomas,3 cases of chromophobe renal cell carcinomas,one case of papillary carcinoma.Conclusions Dual laser system can be used in off clamp nephron-sparing surgery safely and effectively.

Objective To discuss the MRI features of pyometra and its correlation with pathologic.Methods MRI and DWI data of 12 cases pyometra proved by surgery and pathology were analyzed retrospectively, and a comparision with pathologic findings was made.Mean ADC values were calculated.Results According to the homogeneity of MRI signal: Uniform signal type in 2 cases,T1WI showed slightly low signal,T2WI showed slightly high signal,DWI showed high signal,and the distribution of pus compositions is relatively uniform;Signal mixed type in 5 cases, T2 showed at the bottom of the pus or peripheral mixed slightly short signal, DWI showed mixed high signal, there was some sediment in the bottom of the pus;5 cases of liquid layer type, the upper displayed signal of water,and the lower signal was lower than the upper signal on T2WI, of which 3 cases showed uniform signal of the lower, DWI showed a uniform high signal, the distribution of pus compositions is uniform, the other 2 cases showed mixed signal of the lower, which was pyometra with bleeding,DWI showed high signal and low signal mixed together.The average ADC value of the 12 patients were 0.532×10-3 mm2/s.12 cases of uterine volume were increased, including 10 cases of uterine wall thinning, the other 2 cases with inflammatory invasion, the uterine wall thickening.8 cases with cervical cancer.5 cases with pelvic effusion.Conclusion MRI findings of pyometra are characteristic, its MRI manifestations and pathological components are highly correlated,and the ADC value is of great value in the diagnosis of pyometra.

Background and purpose: It has been reported that epidermal growth factor receptor (EGFR) and epidermal growth factor receptor variant Ⅲ (EGFRv Ⅲ) play important roles in the progression of various cancers. This research was to detect the expression and relation of EGFR and epidermal growth factor receptor variant Ⅲ (EGFRv Ⅲ) to human esophageal carcinoma. Methods: In 66 human esophageal carcinoma tissues, the expression of EGFR and EGFRv Ⅲ were detected by imrnunohistochemistry and western-blot. The expression of EGFR and EGFRv Ⅲ along with the patients' clinicopathologic factors was retrospectively analyzed. Correlation analysis between EGFRv Ⅲ and EGFR was analyzed by Pearson correlation coefficient. Results: The average gray scale values of EGFR in esophageal carcinoma and normal tissues by immunohistochemistry were 25.4±3.2 and 5.0±3.5, which showed a significant difference (t=5.574, P=0.000). And the average gray scale values of EGFRv Ⅲ in esophageal carcinoma and normal tissues were 22.5±4.2 and 5.5±3.0, which also showed a significant difference (t=6.701,P=0.000). The average gray scale values of EGFR in esophageal carcinoma and normal tissues respectively by western-blot were 1.37±0.41 and 0.21±0.09, which showed a significant difference (t=10.704, P=0.000) And the average gray scale values of EGFRv Ⅲ respectively were 0.828±0.15 and 0.083±0.049, which had a significant difference (t=9.362, P=0.000). Significant differences were observed in TNM-stage, lymphatic metastasis and tumor classification in both the expression of EGFR (P<0.05) and EGFRv Ⅲ (P<0.05), and but there were no obvious differences in gender, age, minor size, growth pattern in both the expression of EGFR (P<0.05) and EGFRvⅢ (P<0.05). Correlation analysis showed that there was strong association of the expression between EGFR and EGFRv Ⅲ both detected by immunohistochemistry (r=0.701,P<0.0001) and western-blot respectively(r=0.556, P=0.031). Conclusion: Our data suggests that EGFRvⅢ is over-expressed in human esophageal carcinoma. Combination of EGFR and EGFRvⅢ could be useful markers for tumorgenesis and differentiation of human esophageal carcinoma.

AIM: To study the effect of IL-12 on T lymphocytes apoptosis, the expression of Fas/FasL and TNFR/TNF?. METHODS: Terminal dUTP nick end labeling(TUNEL) and Annexin V assay were used. Anti-TNFR were labeled with FITC, anti-CD95 was labeled with PE and Anti-FasL with biotin. Three kinds of T cells (HTB176,TIB152 and human normal T cells) were analysed through flow cytometry. RESULTS: At 1st hour after being treated with IL-12, the expression of FasL protein and FasL mRNA in HTB176 and TIB152 began to increase and reached peak value in 24 hours. In the normal T cells, FasL just began to increase in 1 hour and maintained stability in 6, 12 and 24 hours through the later experiment period. All three kinds of T cells displayed no change in the expression of CD95 and TNFR/TNF? under the stimulation of IL-12. CONCLUSION: Expression of such apoptosis regulating factors were different in the apoptosis of T cells induced by IL-12.