Objective: The aim of the research was to test the applicability of Chinese Meaning in Life Questionnaire (C-MLQ) and its reliability and validity in college students. Methods: C-MLQ and a parcel of scales were administered to 531 college students from three universities in China. Exploratory Factor Analysis (EFA) and confirmatory factor analysis (CFA) were employed to check the construct validity. Results: Firstly, the internal consistency of the two subscales were qualified, the coefficient alpha of two subscales were 0.85 and 0.82 respectively; test-retest reliability were 0.74 and 0.76 respectively; secondly, compared with original scales, the construct of C-MLQ also had two factors, the item factor loadings ranging from 0.61 to 0.78 on the factor it belonged to. Thirdly, the correlations of C-MLQ and criterion measurement were 0.025 to 0.637. Finally, there were no gender difference in the scores of two subscales. Conclusion: The CMILQ has good reliability and validity and it can be used to assess individual meaning in life.

Transsexuals are specific humans who abandon their traditionally functional reproduction.However they may wish to have a child in the new-gender identity.At present in China,assisted reproductive technology(ART) should be limited to those female-to-male transsexuals with a female partner.The assisted reproductive institutions should keep the information for transsexuals confidential in the whole treatment procedure.In some cases,we could appeal to the ART ethics committee for support.Before sex reassignment surgery(SRS),the candidate transsexuals could preserve their fertility by cryo-storing germ cells,tissues and organs for future reproductive usage.

Objective To investigate the application of serum soluble interleukin-2 receptor(sIL-2R)and soluble tumor necrosis factor receptor Ⅱ (sTNFR Ⅱ) in extrapulmonary organ involvement sarcoidosis.Methods Of 28 cases with sarcoidosis which were in active period,12 cases were in extrapulmonary organ involvement subgroup,16 cases were in pure pulmonary lesions subgroup.Serum sIL-2R and sTNFR Ⅱ of the two groups were detected by enzyme linked immunosorbent assay and compared.Results The expression level of sIL-2R in extrapulmonary organ involvement subgroup was (1.60 ± 0.80) μg/L,significantly higher than that of pure pulmonary lesions group,and there was significant difference((1.00±0.29)μg/L,t =2.764,P＜0.05);and the expression level of sTNFR Ⅱ in extrapulmonary organ involvement subgroup was (1.09 ± 0.24) μg/L,significantly higher than that of pure pulmonary lesions group,and the difference between the two groups was statistically significant((0.85±0.29) μg/L,t =2.183,P＜0.05).Conclusion The expression level of peripheral blood serum sIL-2R and sTNFR Ⅱ may serve as the important indexes to judge the extrapulmonary organ involvement sarcoidosis.

Objective To compare the different expression patterns of survivin and caspase-3 between the larger part and the smaller part of the dissymmetric enlarged prostates in BPH patients, and to clarify the role of apoptosis in BPH etiology. Methods BPH tissues from 25 patients were embedded in paraffin. The specimens constituted of two prostate parts from each patient. One part was from the larger enlarged gland and the other was from the smaller enlarged counterpart. 50 paraf-fin blocks were cut into sections and stained with survivin and caspase-3 polyclonal antibodies against human. All the data was analyzed by SPSS13.0 software. Results Survivin immunoreactivity was localized in prostatic cell nuclei. Survivin expression in larger enlarged parts (22.08±16. 33)0% was significantly higher than the smaller parts (7. 645±5. 45)%(P<0.01). Positive nuclear or cytoplasm staining was also observed in the Caspase-3 immunoreactivity. Caspase-3 expression was higher in smaller enlarged parts (51. 525±27. 27%) than in larger enlarged parts (33. 125±21.01%) (P< 0.01). Survivin expression in larger part of the enlarged prostates was inversely correlated with caspase-3 expression (r=-0. 474, P<0. 05). However, in smaller part of the enlarged prostates, the expression of the two factors had no relation (r=-0. 313,P>0. 05). Conclusions Survivin and caspase-3 expression patterns are different in the two parts of the dissymmetric enlarged prostates. These different patterns indicate apoptosis playing an important role in BPH development.

BACKGROUND: The free radicals induced by cerebral ischemia/reperfusion consist mainly of xanthine oxidase, which induces cell swelling in the infarcted area.OBJECTIVE: To observe the changes of cerebral ischemia/reperfusioninduced changes in the activity of cerebral superoxide dismutase (SOD), an enzyme responsible for free radical clearance, and investigate the effect of apurin, a inhibitor of purine oxidase, on cellular water content in the brain tissue with ischemia/reperfusion injury.DESIGN: Completely randomized controlled study.SETTING: Department of Neurology of the Central Hospital Affiliated to Shenyang Medical College, Department of Neurosurgery of the First Hospital Affiliated to China Medical University, and Liaoning Provincial Orthopedic Hospital for Limb Disabilities.MATERIALS: The experiment was conducted in the Laboratory of the Central Hospital Affiliated to Shenyang Medical College from May 2003 to April 2004. Forty Wistar rats were subjected to a 6-hour cerebral ischemia and randomized into 4 equal groups to receive intragastric administration of 100 mg/kg apurin (ischemia + apurin group), oxolinic acid suspension of the same dose (ischemia+ oxolinic acid group), 100 mg/kg apurin after a 2-hour reperfusion (Ischemia/reperfusion + apurin group), or oxolinic acid of the same dosage after the 2-hour reperfusion (ischemia/reperfusion + oxolinic acid goup), respectively. The rats in apurin group had intragastric administration of 100 mg/kg apurin 48, 24 and 1 hour before occlusion of the cervical internal carotid artery (CICA) to induce the ischemia, respectively. Oxolinic acid was given in the two oxolinic acid groups in the same manner.METHODS:Water content of brain tissue of rats was measured after 6 hours of CICA occlusion in the two ischemia groups and after the 2-hour perfusion in the two ischemia/reperfusion groups. Distribution of SOD in the brain tissue was observed with SOD immunostaining.MAIN OUTCOME MEASURES: Distribution of SOD and water content in the brain tissue of rats.RESULTS: In the two oxolinic acid groups, Cu-Zn SOD staining identified obviously increased staining intensity in the ischemic foci. Mn SOD staining in ischemia+oxolinic acid group resulted in increased circular staining surrounding the vessels in the ischemic foci, with also obvious staining of the vascular wall and neural cells. The ischemic foci of the ischemia/reperfusion + oxolinic acid group showed diffuse but lightly weaker staining. Cu-Zn SOD staining in the two apurin groups revealed no significant difference. In the two oxolinic acid groups, endothelial cell nuclear swelling of the arteriole, protrusion of the mid-layer myocytes, and expansion of the vascular membrane were observed, with the tissues surrounding the vessels appearing spongy. These changes were less severe in the two apurin groups. The water content in the brain tissue was (78.56±0.30) % in ischemia + apurin group and (78.85±0.49) % in ischemia/reperfusion + apurin group, significantly lower than that of (79.08±0.33) % in ischemia + oxolinic acid group and (79.86±0.49) % in ischemia/reperfusion + oxolinic acid group (P ＜ 0.05).CONCLUSION: Apurin can relieve tissue injury after cerebral ischemia/reperfusion by inhibition of SOD.

With a view to some common problems and characteristics of medicine graduate students education,how to intensify the moral education work of medicine graduate students under the new situation is researched and discussed,and some countermeasures and suggestion are put forward in this article.to systemically organize the ideological and political education work for freshmen;to exert the theory guidance of medical ethnics in moral education of graduate students;to intensify education and management in graduate students,and fully play the education role in management and serving;to adopt detailed measures to promote the performance of tutors and specialty departments in teaching,scientific research and academic education;to include medical social practice of poor-salvation into moral education program for medicine graduate students.

Objective: To investigate the factor structure of the PTSD through analyzing the factor structure of the PTSD Checklist in adolescents from earthquake disaster region. Methods: Chinese PTSD Checklist-Civilian Version was used to assess 560 adolescents, and technique of Confirmatory Factor Analysis (CFA) was employed to comparing seven competitive models. Results: The intercorrelated four-factor model was the best fit one. Conclusion: The Chinese PTSD Checklist-Civilian has an intercorrelated four-factor model; intercorrelated four-factor model of the PTSD may fit the Chinese people, however, which needs further confirmation.

Objective: The purpose of the current research was to explore the relationship between delay behavior and time perspective of college students. Methods: 456 college students (including 135 graduate students), from four Chinese universities, were surveyed with Aitken Procrastination Inventory (API) and Time Perspective Scale for Adolescent. Results: Firstly, there didn’t exist significant difference between different genders and grades; secondly, the scores of API were significantly correlated with length factor of Future Time Perspective (r=0.55, P

Objective To observe anti-sense phosphorothioate oligonucletide (ASPSODN) targeted directly to hTERT mRNA to its inhibiting effect on aimed gene and the influence on the telomerase activity, cellular proliferation, cell apoptosis of K562 cells. Methods Human leukemia cell line K562 was transfected with anti-sense oligonucleotide ASPSODN by liposome. The proliferation activity of K562 cell line was determined by using methyl thiazolyl tetrazolium assay, and telomerase activity was detected by TRAP-PCR-ELISA. Flow cytometry was adopted to examine apoptotic rate and cell cycle. RT-PCR was used to detect the expression of target gene hTERT mRNA. Results 0.6 μmol/L ASPSODN (0.42 ±0.16) was remarkably decreased the expression of hTERT mRNA, Telomerase relative activation was decreased by 52 %. According to 0.6 (μmol/L ASPSODN caused significant the inhibition of K562 cell growth. Apoptotic rate and cell cycle was examined by 0.6 μmol/L ASPSODN with flow cytometry. The cell apoptosis rate of 0.6μmol/L ASPSODN were 10.31 %. It showed the cells treated with 0.6 uU PSASODN arrested in G_1/C_0. The ratio of cells in G_2/M and S period was reduced. But there was no characteristic apoptosis peak. Conclusion ASPSODN targeted hTERT can inhibit the expression of target gene hTERT mRNA, and decrease the telomerase activity of K562 cells. ASPSODN can inhibit strongly the proliferation of K562 cell and induce cell apoptosis by decreasing telomerase activity.

Objective To investigate the expression of proliferating cell nuclear antigen(PCNA),vascular endothelial growth factor(VEGF) and microvessel density(MVD) in squamous cell carcinoma;and to explore the relationship between their expression and tumor biological behavior.Methods The expressions of PCNA,VEGF and MVD were analyzed by immunohistochemical method in 71 cases of esophageal squamous cell carcinoma.Results The intensity positive rate of PCNA indicated close relationship between cell differentiation and lymph node metastasis(P